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Protein

Hydroxyethylthiazole kinase

Gene

thiM

Organism
Lactobacillus sakei subsp. sakei (strain 23K)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Hydroxyethylthiazole kinase (thiM)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei41Substrate; via amide nitrogenUniRule annotation1
Binding sitei117ATPUniRule annotation1
Binding sitei163ATPUniRule annotation1
Binding sitei190Substrate; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processThiamine biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLSAK314315:LCA_RS00285-MONOMER
UniPathwayiUPA00060; UER00139

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyethylthiazole kinaseUniRule annotation (EC:2.7.1.50UniRule annotation)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinaseUniRule annotation
Short name:
TH kinaseUniRule annotation
Short name:
Thz kinaseUniRule annotation
Gene namesi
Name:thiMUniRule annotation
Ordered Locus Names:LCA_0059
OrganismiLactobacillus sakei subsp. sakei (strain 23K)
Taxonomic identifieri314315 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
Proteomesi
  • UP000002707 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000215151 – 269Hydroxyethylthiazole kinaseAdd BLAST269

Interactioni

Protein-protein interaction databases

STRINGi314315.LSA0059

Structurei

3D structure databases

ProteinModelPortaliQ38ZL9
SMRiQ38ZL9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C4U Bacteria
COG2145 LUCA
KOiK00878
OMAiATGQKDF
OrthoDBiPOG091H004S

Family and domain databases

CDDicd01170 THZ_kinase, 1 hit
Gene3Di3.40.1190.20, 1 hit
HAMAPiMF_00228 Thz_kinase, 1 hit
InterProiView protein in InterPro
IPR000417 Hyethyz_kinase
IPR029056 Ribokinase-like
PANTHERiPTHR44423 PTHR44423, 1 hit
PfamiView protein in Pfam
PF02110 HK, 1 hit
PIRSFiPIRSF000513 Thz_kinase, 1 hit
PRINTSiPR01099 HYETHTZKNASE
SUPFAMiSSF53613 SSF53613, 1 hit

Sequencei

Sequence statusi: Complete.

Q38ZL9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKTLLDQIK QANPIITNVA NSVTVDQVAN VQNIIGASPI MSSDPEEAPE
60 70 80 90 100
MVAIAQALSI NIGTLSTEPI HQMKTLMAEA YRQAKPVVID PVAVSSIHYR
110 120 130 140 150
QKIIDELLAL GTPQIIRGNA GEIAYLAGLD WQANGIDAGS GEIDLVKVAQ
160 170 180 190 200
TAAKKQQTTI LLSGPTDIIT DGHHTTKVAN GTPLFQVHVG SGDMLTGLCA
210 220 230 240 250
AFVAVSPDNP YQAAIDAATT FAVAGQLVAE AMPTPLPGSF YPQLLDCLFN
260
ITAADVQTHA QVTEVLTHE
Length:269
Mass (Da):28,312
Last modified:November 22, 2005 - v1
Checksum:i1448AA99CB9480FB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR936503 Genomic DNA Translation: CAI54358.1
RefSeqiWP_011373773.1, NC_007576.1

Genome annotation databases

EnsemblBacteriaiCAI54358; CAI54358; LCA_0059
GeneIDi29636518
KEGGilsa:LCA_0059

Similar proteinsi

Entry informationi

Entry nameiTHIM_LACSS
AccessioniPrimary (citable) accession number: Q38ZL9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 22, 2005
Last modified: June 20, 2018
This is version 75 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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