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Q38YS5

- TSAD_LACSS

UniProt

Q38YS5 - TSAD_LACSS

Protein

tRNA N6-adenosine threonylcarbamoyltransferase

Gene

tsaD

Organism
Lactobacillus sakei subsp. sakei (strain 23K)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 61 (01 Oct 2014)
      Sequence version 1 (22 Nov 2005)
      Previous versions | rss
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    Functioni

    Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction.UniRule annotation

    Catalytic activityi

    L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

    Cofactori

    Binds 1 Fe2+ ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi116 – 1161IronUniRule annotation
    Metal bindingi120 – 1201IronUniRule annotation
    Binding sitei171 – 1711SubstrateUniRule annotation
    Binding sitei184 – 1841Substrate; via amide nitrogenUniRule annotation
    Binding sitei188 – 1881SubstrateUniRule annotation
    Binding sitei277 – 2771SubstrateUniRule annotation
    Metal bindingi307 – 3071IronUniRule annotation

    GO - Molecular functioni

    1. iron ion binding Source: UniProtKB-HAMAP
    2. metalloendopeptidase activity Source: InterPro
    3. transferase activity, transferring acyl groups other than amino-acyl groups Source: UniProtKB-HAMAP

    GO - Biological processi

    1. threonylcarbamoyladenosine biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    Iron, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.6.99.4UniRule annotation)
    Alternative name(s):
    N6-L-threonylcarbamoyladenine synthaseUniRule annotation
    Short name:
    t(6)A synthaseUniRule annotation
    t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation
    tRNA threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation
    Gene namesi
    Name:tsaDUniRule annotation
    Synonyms:gcp
    Ordered Locus Names:LCA_0351
    OrganismiLactobacillus sakei subsp. sakei (strain 23K)
    Taxonomic identifieri314315 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
    ProteomesiUP000002707: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 344344tRNA N6-adenosine threonylcarbamoyltransferasePRO_0000303397Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi314315.LSA0351.

    Structurei

    3D structure databases

    ProteinModelPortaliQ38YS5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni138 – 1425Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the KAE1 / TsaD family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0533.
    KOiK01409.
    OMAiFQASVVE.
    OrthoDBiEOG6K402S.

    Family and domain databases

    HAMAPiMF_01445. TsaD.
    InterProiIPR000905. Gcp-like_dom.
    IPR017861. KAE1/YgjD.
    IPR017860. Peptidase_M22_CS.
    IPR022450. TsaD.
    [Graphical view]
    PfamiPF00814. Peptidase_M22. 1 hit.
    [Graphical view]
    PRINTSiPR00789. OSIALOPTASE.
    TIGRFAMsiTIGR00329. gcp_kae1. 1 hit.
    TIGR03723. T6A_YgjD. 1 hit.
    PROSITEiPS01016. GLYCOPROTEASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q38YS5-1 [UniParc]FASTAAdd to Basket

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    MTEKKELILA FESSCDETSV AVIENGQRIL SNVIATQIKS HQRFGGVVPE    50
    VASRHHVEQI TLCTQEALEQ AGVTYDDLTA VAVTYGPGLV GALLIGVTAA 100
    KAIAYAHHLP LVPVNHMAGH IYAARFVKPL EYPLLALLVS GGHTELVYMP 150
    AAGQFEIIGD TRDDAAGEAY DKIGRVLGVP YPAGKEIDRL AHLGQDTFNF 200
    PRAMLKEDNL DFSFSGLKSA FINTVHHADQ IGETLDQADL AASFQASVVE 250
    VLVTKTLRAA QSLKVKQLVV AGGVAANQGL REGLAAGIES AGLDLDLIMP 300
    PLRLCGDNGA MIGAAAHIAL AQNTLADLDL NAIPSLDFPY QNEL 344
    Length:344
    Mass (Da):36,536
    Last modified:November 22, 2005 - v1
    Checksum:i092FB9919050EE2D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR936503 Genomic DNA. Translation: CAI54652.1.
    RefSeqiWP_011374060.1. NC_007576.1.
    YP_394964.1. NC_007576.1.

    Genome annotation databases

    EnsemblBacteriaiCAI54652; CAI54652; LCA_0351.
    GeneIDi3778789.
    KEGGilsa:LSA0351.
    PATRICi22273470. VBILacSak116432_0343.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR936503 Genomic DNA. Translation: CAI54652.1 .
    RefSeqi WP_011374060.1. NC_007576.1.
    YP_394964.1. NC_007576.1.

    3D structure databases

    ProteinModelPortali Q38YS5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 314315.LSA0351.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAI54652 ; CAI54652 ; LCA_0351 .
    GeneIDi 3778789.
    KEGGi lsa:LSA0351.
    PATRICi 22273470. VBILacSak116432_0343.

    Phylogenomic databases

    eggNOGi COG0533.
    KOi K01409.
    OMAi FQASVVE.
    OrthoDBi EOG6K402S.

    Family and domain databases

    HAMAPi MF_01445. TsaD.
    InterProi IPR000905. Gcp-like_dom.
    IPR017861. KAE1/YgjD.
    IPR017860. Peptidase_M22_CS.
    IPR022450. TsaD.
    [Graphical view ]
    Pfami PF00814. Peptidase_M22. 1 hit.
    [Graphical view ]
    PRINTSi PR00789. OSIALOPTASE.
    TIGRFAMsi TIGR00329. gcp_kae1. 1 hit.
    TIGR03723. T6A_YgjD. 1 hit.
    PROSITEi PS01016. GLYCOPROTEASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 23K.

    Entry informationi

    Entry nameiTSAD_LACSS
    AccessioniPrimary (citable) accession number: Q38YS5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 11, 2007
    Last sequence update: November 22, 2005
    Last modified: October 1, 2014
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3