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Protein

tRNA N6-adenosine threonylcarbamoyltransferase

Gene

tsaD

Organism
Lactobacillus sakei subsp. sakei (strain 23K)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction.UniRule annotation

Catalytic activityi

L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N6-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

Fe2+UniRule annotationNote: Binds 1 Fe2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi116IronUniRule annotation1
Metal bindingi120IronUniRule annotation1
Binding sitei171SubstrateUniRule annotation1
Binding sitei184Substrate; via amide nitrogenUniRule annotation1
Binding sitei188SubstrateUniRule annotation1
Binding sitei277SubstrateUniRule annotation1
Metal bindingi307IronUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processtRNA processing
LigandIron, Metal-binding

Enzyme and pathway databases

BioCyciLSAK314315:G1GI3-335-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.3.1.234UniRule annotation)
Alternative name(s):
N6-L-threonylcarbamoyladenine synthaseUniRule annotation
Short name:
t(6)A synthaseUniRule annotation
t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation
tRNA threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation
Gene namesi
Name:tsaDUniRule annotation
Synonyms:gcp
Ordered Locus Names:LCA_0351
OrganismiLactobacillus sakei subsp. sakei (strain 23K)
Taxonomic identifieri314315 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
Proteomesi

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003033971 – 344tRNA N6-adenosine threonylcarbamoyltransferaseAdd BLAST344

Interactioni

Protein-protein interaction databases

STRINGi314315.LSA0351

Structurei

3D structure databases

ProteinModelPortaliQ38YS5
SMRiQ38YS5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni138 – 142Substrate bindingUniRule annotation5

Sequence similaritiesi

Belongs to the KAE1 / TsaD family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CPM Bacteria
COG0533 LUCA
KOiK01409
OMAiHLEGHIY
OrthoDBiPOG091H010B

Family and domain databases

HAMAPiMF_01445 TsaD, 1 hit
InterProiView protein in InterPro
IPR000905 Gcp-like_dom
IPR017861 KAE1/TsaD
IPR017860 Peptidase_M22_CS
IPR022450 TsaD
PANTHERiPTHR11735 PTHR11735, 1 hit
PTHR11735:SF6 PTHR11735:SF6, 1 hit
PfamiView protein in Pfam
PF00814 Peptidase_M22, 1 hit
PRINTSiPR00789 OSIALOPTASE
TIGRFAMsiTIGR00329 gcp_kae1, 1 hit
TIGR03723 T6A_TsaD_YgjD, 1 hit
PROSITEiView protein in PROSITE
PS01016 GLYCOPROTEASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q38YS5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEKKELILA FESSCDETSV AVIENGQRIL SNVIATQIKS HQRFGGVVPE
60 70 80 90 100
VASRHHVEQI TLCTQEALEQ AGVTYDDLTA VAVTYGPGLV GALLIGVTAA
110 120 130 140 150
KAIAYAHHLP LVPVNHMAGH IYAARFVKPL EYPLLALLVS GGHTELVYMP
160 170 180 190 200
AAGQFEIIGD TRDDAAGEAY DKIGRVLGVP YPAGKEIDRL AHLGQDTFNF
210 220 230 240 250
PRAMLKEDNL DFSFSGLKSA FINTVHHADQ IGETLDQADL AASFQASVVE
260 270 280 290 300
VLVTKTLRAA QSLKVKQLVV AGGVAANQGL REGLAAGIES AGLDLDLIMP
310 320 330 340
PLRLCGDNGA MIGAAAHIAL AQNTLADLDL NAIPSLDFPY QNEL
Length:344
Mass (Da):36,536
Last modified:November 22, 2005 - v1
Checksum:i092FB9919050EE2D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR936503 Genomic DNA Translation: CAI54652.1
RefSeqiWP_011374060.1, NC_007576.1

Genome annotation databases

EnsemblBacteriaiCAI54652; CAI54652; LCA_0351
GeneIDi29637973
KEGGilsa:LCA_0351

Similar proteinsi

Entry informationi

Entry nameiTSAD_LACSS
AccessioniPrimary (citable) accession number: Q38YS5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: November 22, 2005
Last modified: May 23, 2018
This is version 82 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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