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Q38YS5 (TSAD_LACSS) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA N6-adenosine threonylcarbamoyltransferase

EC=2.3.1.-
Alternative name(s):
t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaD
tRNA threonylcarbamoyladenosine biosynthesis protein TsaD
Gene names
Name:tsaD
Synonyms:gcp
Ordered Locus Names:LCA_0351
OrganismLactobacillus sakei subsp. sakei (strain 23K) [Complete proteome] [HAMAP]
Taxonomic identifier314315 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length344 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction By similarity. HAMAP-Rule MF_01445

Cofactor

Binds 1 Fe2+ ion per subunit By similarity. HAMAP-Rule MF_01445

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01445.

Sequence similarities

Belongs to the KAE1 / TsaD family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   LigandIron
Metal-binding
   Molecular functionAcyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processthreonylcarbamoyladenosine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioniron ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metalloendopeptidase activity

Inferred from electronic annotation. Source: InterPro

transferase activity, transferring acyl groups other than amino-acyl groups

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 344344tRNA N6-adenosine threonylcarbamoyltransferase HAMAP-Rule MF_01445
PRO_0000303397

Regions

Region138 – 1425Substrate binding By similarity

Sites

Metal binding1161Iron By similarity
Metal binding1201Iron By similarity
Metal binding3071Iron By similarity
Binding site1711Substrate By similarity
Binding site1841Substrate; via amide nitrogen By similarity
Binding site1881Substrate By similarity
Binding site2771Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q38YS5 [UniParc].

Last modified November 22, 2005. Version 1.
Checksum: 092FB9919050EE2D

FASTA34436,536
        10         20         30         40         50         60 
MTEKKELILA FESSCDETSV AVIENGQRIL SNVIATQIKS HQRFGGVVPE VASRHHVEQI 

        70         80         90        100        110        120 
TLCTQEALEQ AGVTYDDLTA VAVTYGPGLV GALLIGVTAA KAIAYAHHLP LVPVNHMAGH 

       130        140        150        160        170        180 
IYAARFVKPL EYPLLALLVS GGHTELVYMP AAGQFEIIGD TRDDAAGEAY DKIGRVLGVP 

       190        200        210        220        230        240 
YPAGKEIDRL AHLGQDTFNF PRAMLKEDNL DFSFSGLKSA FINTVHHADQ IGETLDQADL 

       250        260        270        280        290        300 
AASFQASVVE VLVTKTLRAA QSLKVKQLVV AGGVAANQGL REGLAAGIES AGLDLDLIMP 

       310        320        330        340 
PLRLCGDNGA MIGAAAHIAL AQNTLADLDL NAIPSLDFPY QNEL 

« Hide

References

[1]"The complete genome sequence of the meat-borne lactic acid bacterium Lactobacillus sakei 23K."
Chaillou S., Champomier-Verges M.-C., Cornet M., Crutz-Le Coq A.-M., Dudez A.-M., Martin V., Beaufils S., Darbon-Rongere E., Bossy R., Loux V., Zagorec M.
Nat. Biotechnol. 23:1527-1533(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 23K.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR936503 Genomic DNA. Translation: CAI54652.1.
RefSeqYP_394964.1. NC_007576.1.

3D structure databases

ProteinModelPortalQ38YS5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING314315.LSA0351.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAI54652; CAI54652; LCA_0351.
GeneID3778789.
KEGGlsa:LSA0351.
PATRIC22273470. VBILacSak116432_0343.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0533.
KOK01409.
OMAQLMRVDG.
OrthoDBEOG6K402S.
ProtClustDBPRK09604.

Family and domain databases

HAMAPMF_01445. TsaD.
InterProIPR000905. Gcp-like_dom.
IPR017861. KAE1/YgjD.
IPR017860. Peptidase_M22_CS.
IPR022450. TsaD.
[Graphical view]
PfamPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PRINTSPR00789. OSIALOPTASE.
TIGRFAMsTIGR00329. gcp_kae1. 1 hit.
TIGR03723. T6A_YgjD. 1 hit.
PROSITEPS01016. GLYCOPROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTSAD_LACSS
AccessionPrimary (citable) accession number: Q38YS5
Entry history
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: November 22, 2005
Last modified: March 19, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families