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Q38YS5

- TSAD_LACSS

UniProt

Q38YS5 - TSAD_LACSS

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Protein
tRNA N6-adenosine threonylcarbamoyltransferase
Gene
tsaD, gcp, LCA_0351
Organism
Lactobacillus sakei subsp. sakei (strain 23K)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction By similarity.UniRule annotation

Catalytic activityi

L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

Binds 1 Fe2+ ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi116 – 1161Iron By similarity
Metal bindingi120 – 1201Iron By similarity
Binding sitei171 – 1711Substrate By similarity
Binding sitei184 – 1841Substrate; via amide nitrogen By similarity
Binding sitei188 – 1881Substrate By similarity
Binding sitei277 – 2771Substrate By similarity
Metal bindingi307 – 3071Iron By similarity

GO - Molecular functioni

  1. iron ion binding Source: UniProtKB-HAMAP
  2. metalloendopeptidase activity Source: InterPro
  3. transferase activity, transferring acyl groups other than amino-acyl groups Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. threonylcarbamoyladenosine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA N6-adenosine threonylcarbamoyltransferase (EC:2.6.99.4)
Alternative name(s):
N6-L-threonylcarbamoyladenine synthase
Short name:
t(6)A synthase
t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaD
tRNA threonylcarbamoyladenosine biosynthesis protein TsaD
Gene namesi
Name:tsaD
Synonyms:gcp
Ordered Locus Names:LCA_0351
OrganismiLactobacillus sakei subsp. sakei (strain 23K)
Taxonomic identifieri314315 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000002707: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 344344tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation
PRO_0000303397Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi314315.LSA0351.

Structurei

3D structure databases

ProteinModelPortaliQ38YS5.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni138 – 1425Substrate binding By similarity

Sequence similaritiesi

Belongs to the KAE1 / TsaD family.

Phylogenomic databases

eggNOGiCOG0533.
KOiK01409.
OMAiFQASVVE.
OrthoDBiEOG6K402S.

Family and domain databases

HAMAPiMF_01445. TsaD.
InterProiIPR000905. Gcp-like_dom.
IPR017861. KAE1/YgjD.
IPR017860. Peptidase_M22_CS.
IPR022450. TsaD.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PRINTSiPR00789. OSIALOPTASE.
TIGRFAMsiTIGR00329. gcp_kae1. 1 hit.
TIGR03723. T6A_YgjD. 1 hit.
PROSITEiPS01016. GLYCOPROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q38YS5-1 [UniParc]FASTAAdd to Basket

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MTEKKELILA FESSCDETSV AVIENGQRIL SNVIATQIKS HQRFGGVVPE    50
VASRHHVEQI TLCTQEALEQ AGVTYDDLTA VAVTYGPGLV GALLIGVTAA 100
KAIAYAHHLP LVPVNHMAGH IYAARFVKPL EYPLLALLVS GGHTELVYMP 150
AAGQFEIIGD TRDDAAGEAY DKIGRVLGVP YPAGKEIDRL AHLGQDTFNF 200
PRAMLKEDNL DFSFSGLKSA FINTVHHADQ IGETLDQADL AASFQASVVE 250
VLVTKTLRAA QSLKVKQLVV AGGVAANQGL REGLAAGIES AGLDLDLIMP 300
PLRLCGDNGA MIGAAAHIAL AQNTLADLDL NAIPSLDFPY QNEL 344
Length:344
Mass (Da):36,536
Last modified:November 22, 2005 - v1
Checksum:i092FB9919050EE2D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR936503 Genomic DNA. Translation: CAI54652.1.
RefSeqiWP_011374060.1. NC_007576.1.
YP_394964.1. NC_007576.1.

Genome annotation databases

EnsemblBacteriaiCAI54652; CAI54652; LCA_0351.
GeneIDi3778789.
KEGGilsa:LSA0351.
PATRICi22273470. VBILacSak116432_0343.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR936503 Genomic DNA. Translation: CAI54652.1 .
RefSeqi WP_011374060.1. NC_007576.1.
YP_394964.1. NC_007576.1.

3D structure databases

ProteinModelPortali Q38YS5.
ModBasei Search...

Protein-protein interaction databases

STRINGi 314315.LSA0351.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAI54652 ; CAI54652 ; LCA_0351 .
GeneIDi 3778789.
KEGGi lsa:LSA0351.
PATRICi 22273470. VBILacSak116432_0343.

Phylogenomic databases

eggNOGi COG0533.
KOi K01409.
OMAi FQASVVE.
OrthoDBi EOG6K402S.

Family and domain databases

HAMAPi MF_01445. TsaD.
InterProi IPR000905. Gcp-like_dom.
IPR017861. KAE1/YgjD.
IPR017860. Peptidase_M22_CS.
IPR022450. TsaD.
[Graphical view ]
Pfami PF00814. Peptidase_M22. 1 hit.
[Graphical view ]
PRINTSi PR00789. OSIALOPTASE.
TIGRFAMsi TIGR00329. gcp_kae1. 1 hit.
TIGR03723. T6A_YgjD. 1 hit.
PROSITEi PS01016. GLYCOPROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 23K.

Entry informationi

Entry nameiTSAD_LACSS
AccessioniPrimary (citable) accession number: Q38YS5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: November 22, 2005
Last modified: September 3, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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