Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q38YS5

- TSAD_LACSS

UniProt

Q38YS5 - TSAD_LACSS

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

tRNA N6-adenosine threonylcarbamoyltransferase

Gene

tsaD

Organism
Lactobacillus sakei subsp. sakei (strain 23K)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction.UniRule annotation

Catalytic activityi

L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

Binds 1 Fe2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi116 – 1161IronUniRule annotation
Metal bindingi120 – 1201IronUniRule annotation
Binding sitei171 – 1711SubstrateUniRule annotation
Binding sitei184 – 1841Substrate; via amide nitrogenUniRule annotation
Binding sitei188 – 1881SubstrateUniRule annotation
Binding sitei277 – 2771SubstrateUniRule annotation
Metal bindingi307 – 3071IronUniRule annotation

GO - Molecular functioni

  1. iron ion binding Source: UniProtKB-HAMAP
  2. metalloendopeptidase activity Source: InterPro
  3. transferase activity, transferring acyl groups other than amino-acyl groups Source: UniProtKB-HAMAP

GO - Biological processi

  1. threonylcarbamoyladenosine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.6.99.4UniRule annotation)
Alternative name(s):
N6-L-threonylcarbamoyladenine synthaseUniRule annotation
Short name:
t(6)A synthaseUniRule annotation
t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation
tRNA threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation
Gene namesi
Name:tsaDUniRule annotation
Synonyms:gcp
Ordered Locus Names:LCA_0351
OrganismiLactobacillus sakei subsp. sakei (strain 23K)
Taxonomic identifieri314315 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000002707: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 344344tRNA N6-adenosine threonylcarbamoyltransferasePRO_0000303397Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi314315.LSA0351.

Structurei

3D structure databases

ProteinModelPortaliQ38YS5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni138 – 1425Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the KAE1 / TsaD family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0533.
KOiK01409.
OMAiFQASVVE.
OrthoDBiEOG6K402S.

Family and domain databases

HAMAPiMF_01445. TsaD.
InterProiIPR000905. Gcp-like_dom.
IPR017861. KAE1/YgjD.
IPR017860. Peptidase_M22_CS.
IPR022450. TsaD.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PRINTSiPR00789. OSIALOPTASE.
TIGRFAMsiTIGR00329. gcp_kae1. 1 hit.
TIGR03723. T6A_YgjD. 1 hit.
PROSITEiPS01016. GLYCOPROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q38YS5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTEKKELILA FESSCDETSV AVIENGQRIL SNVIATQIKS HQRFGGVVPE
60 70 80 90 100
VASRHHVEQI TLCTQEALEQ AGVTYDDLTA VAVTYGPGLV GALLIGVTAA
110 120 130 140 150
KAIAYAHHLP LVPVNHMAGH IYAARFVKPL EYPLLALLVS GGHTELVYMP
160 170 180 190 200
AAGQFEIIGD TRDDAAGEAY DKIGRVLGVP YPAGKEIDRL AHLGQDTFNF
210 220 230 240 250
PRAMLKEDNL DFSFSGLKSA FINTVHHADQ IGETLDQADL AASFQASVVE
260 270 280 290 300
VLVTKTLRAA QSLKVKQLVV AGGVAANQGL REGLAAGIES AGLDLDLIMP
310 320 330 340
PLRLCGDNGA MIGAAAHIAL AQNTLADLDL NAIPSLDFPY QNEL
Length:344
Mass (Da):36,536
Last modified:November 22, 2005 - v1
Checksum:i092FB9919050EE2D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR936503 Genomic DNA. Translation: CAI54652.1.
RefSeqiYP_394964.1. NC_007576.1.

Genome annotation databases

EnsemblBacteriaiCAI54652; CAI54652; LCA_0351.
GeneIDi3778789.
KEGGilsa:LSA0351.
PATRICi22273470. VBILacSak116432_0343.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR936503 Genomic DNA. Translation: CAI54652.1 .
RefSeqi YP_394964.1. NC_007576.1.

3D structure databases

ProteinModelPortali Q38YS5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 314315.LSA0351.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAI54652 ; CAI54652 ; LCA_0351 .
GeneIDi 3778789.
KEGGi lsa:LSA0351.
PATRICi 22273470. VBILacSak116432_0343.

Phylogenomic databases

eggNOGi COG0533.
KOi K01409.
OMAi FQASVVE.
OrthoDBi EOG6K402S.

Family and domain databases

HAMAPi MF_01445. TsaD.
InterProi IPR000905. Gcp-like_dom.
IPR017861. KAE1/YgjD.
IPR017860. Peptidase_M22_CS.
IPR022450. TsaD.
[Graphical view ]
Pfami PF00814. Peptidase_M22. 1 hit.
[Graphical view ]
PRINTSi PR00789. OSIALOPTASE.
TIGRFAMsi TIGR00329. gcp_kae1. 1 hit.
TIGR03723. T6A_YgjD. 1 hit.
PROSITEi PS01016. GLYCOPROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 23K.

Entry informationi

Entry nameiTSAD_LACSS
AccessioniPrimary (citable) accession number: Q38YS5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: November 22, 2005
Last modified: October 29, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3