Reviewed, UniProtKB/Swiss-Prot Q38YF0 (GUAC_LACSS)

Names and origin

Protein names

Recommended name:
    GMP reductase
    EC=1.7.1.7
Alternative name(s):
    Guanosine 5'-monophosphate oxidoreductase
      Short name=Guanosine monophosphate reductase

Gene names

Name:	guaC
Ordered Locus Names:	LCA_0476

Organism

Lactobacillus sakei subsp. sakei (strain 23K) [Complete proteome] [HAMAP]

Taxonomic identifier

314315 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides By similarity. HAMAP MF_01511
Catalytic activity	Inosine 5'-phosphate + NH3 + NADP+ = guanosine 5'-phosphate + NADPH. HAMAP MF_01511
Sequence similarities	Belongs to the IMPDH/GMPR family. GuaC type 2 subfamily.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW purine nucleotide metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	GMP reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 325	325	GMP reductase HAMAP MF_01511		PRO_0000292052
Regions
	Nucleotide binding	203 – 226	24	NADP Potential
Sites
	Active site	174	1	Thioimidate intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q38YF0-1 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: FC050439A34AA543	FASTA	325	36,067
	10 20 30 40 50 60 MDVFDYEDVQ LIPNKCIVKS RSEIDTTVRF GSETFKIPVV PANMQTIIDE PLAIWLAENH 70 80 90 100 110 120 YFYVMHRFQP EKRPAFIKMM HERNLFASIS VGVKDDEFDF INQLAQDNLI PEYITIDIAH 130 140 150 160 170 180 GHSQVVIDMI QHIKKVLPKS FVIAGNVGTP EAVRDLERAG ADATKVGIGP GKVCITKIKT 190 200 210 220 230 240 GFGTGGWQLG ALRWCAKAAT KPIIADGGIR TNGDIAKSIR FGANMVMIGS LFAGHTESPG 250 260 270 280 290 300 ELVEEDGQQF KEYFGSASEF QKGTHQNVEG KRILVPYKGS ISDTLTEMRQ DLQSSISYAG 310 320 GKRLSALRKV DYVLVRHSIF NGDML

References

[1]

"The complete genome sequence of the meat-borne lactic acid bacterium Lactobacillus sakei 23K." Chaillou S., Champomier-Verges M.-C., Cornet M., Crutz-Le Coq A.-M., Dudez A.-M., Martin V., Beaufils S., Darbon-Rongere E., Bossy R., Loux V., Zagorec M. Nat. Biotechnol. 23:1527-1533(2005) [PubMed: 16273110] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR936503 Genomic DNA. Translation: CAI54777.1.
RefSeq	YP_395089.1.
3D structure databases
HSSP	HSSP built from PDB template 1YPF based on UniProtKB Q81JJ9.
SMR	Q38YF0. Positions 2-320.
ModBase	Search...
Protein-protein interaction databases
STRING	Q38YF0.
Genome annotation databases
GeneID	3777651.
GenomeReviews	Gene locus LCA_0476 in contig CR936503_GR.
KEGG	lsa:LSA0476.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0516.
HOGENOM	HBG298985.
OMA	FAGHEES.
PhylomeDB	Q38YF0.
Enzyme and pathway databases
BioCyc	LSAK314315:LSA0476-MONOMER.
Family and domain databases
HAMAP	MF_01511. GMP_reduct_type2. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR005994. GMP_reduct2. IPR015875. IMP_DH/GMP_Rdtase_CS. IPR001093. IMP_DH_GMPRt. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00478. IMPDH. 1 hit. [Graphical view]
PIRSF	PIRSF036500. GMP_red_Firmic. 1 hit.
TIGRFAMs	TIGR01306. GMP_reduct_2. 1 hit.
PROSITE	PS00487. IMP_DH_GMP_RED. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAC_LACSS

Accession

Primary (citable) accession number: Q38YF0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
Last sequence update:	November 22, 2005
Last modified:	February 9, 2010
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)