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Q38XM0

- SYI_LACSS

UniProt

Q38XM0 - SYI_LACSS

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Protein
Isoleucine--tRNA ligase
Gene
ileS, LCA_0756
Organism
Lactobacillus sakei subsp. sakei (strain 23K)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei552 – 5521Aminoacyl-adenylate By similarity
Binding sitei596 – 5961ATP By similarity
Metal bindingi887 – 8871Zinc By similarity
Metal bindingi890 – 8901Zinc By similarity
Metal bindingi907 – 9071Zinc By similarity
Metal bindingi910 – 9101Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:LCA_0756
OrganismiLactobacillus sakei subsp. sakei (strain 23K)
Taxonomic identifieri314315 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000002707: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 928928Isoleucine--tRNA ligaseUniRule annotation
PRO_1000022086Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi314315.LSA0756.

Structurei

3D structure databases

ProteinModelPortaliQ38XM0.
SMRiQ38XM0. Positions 1-915.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionUniRule annotation
Add
BLAST
Motifi593 – 5975"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q38XM0-1 [UniParc]FASTAAdd to Basket

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MRIKETLNLG KTAFPMRAGL PNREIDWQKG WADNNLYQQR QKLNEGKPSF    50
VLHDGPPFAN GNIHMGHALN KTSKDIIVRY KSMNGFRAPF VPGWDTHGLP 100
IEQALAKKGI KRKEMSLVDY RKLCYDYAME QVNTQRQDFK RLGISADWDN 150
PYITLTADFE AEEIRVFGEM AKKGYIYKGK KPVYWSPSSE STLAEAEIEY 200
KDIKSPSMYV AFNVVDGKDL LDADTKFIIW TTTPWTIPAN LGIAVNPAFD 250
YVQVLADGQK YVVAAERLNK MTDLLGWESV EILKTFKGAD MELMTARHPL 300
YDRESLVILG NHVTLETGTG LVHTAPGHGE DDYNAGTKYK LPVLSVVDSK 350
GIMTEDAPGF EGVYYDKANP MVTEALEKNG SLLKLDFFTH SYPHDWRTKK 400
PVIFRATAQW FASIDAFRDQ ILAQIEKVEF MPEWGKTRLY NMIRDRGDWV 450
ISRQRAWGVP LPIFYAEDGT EIITPETIER VAQLFAEHGS NVWFEWDAKD 500
LLPAGFTHPG SPNGEFTKEK DIMDVWFDSG SSHQAVLAAR DELTYPADLI 550
LEGSDQYRGW FNSSLITSVA VGEVSPYKAV ISQGFVLDGN GRKMSKSLGN 600
TILPEKIIKQ MGADIVRLWV ASVDASSDVK VTMENFQQVS EAYRKIRNTM 650
RFMIANTTDF DPAKDTVDYA ELGSVDKFML VRLNAIIESC KAAYDAYDFA 700
TVYKTINMFL TNELSAFYLD FAKDVVYIDG QNDAPRRNMQ TVFYAVAVAL 750
TKLLTPILPH TAEEIWSYLH EPEEFVQLAE MPEVAHFAGE EDLVATWNAF 800
MGIRDDVLKA LETARMDKVI GKSLEAAVTL YPNEANAALL ASLDADVKQL 850
LIVSQLTIAD QAVEAPAEAT QFDGVAVSVA HAEGDVCDRC RMIKTDVGSD 900
DKFPMLCARC AAIVTANYPE AVAEGLEK 928
Length:928
Mass (Da):104,085
Last modified:November 22, 2005 - v1
Checksum:iB603F49C457134AC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR936503 Genomic DNA. Translation: CAI55060.1.
RefSeqiYP_395370.1. NC_007576.1.

Genome annotation databases

EnsemblBacteriaiCAI55060; CAI55060; LCA_0756.
GeneIDi3778601.
KEGGilsa:LSA0756.
PATRICi22274332. VBILacSak116432_0733.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR936503 Genomic DNA. Translation: CAI55060.1 .
RefSeqi YP_395370.1. NC_007576.1.

3D structure databases

ProteinModelPortali Q38XM0.
SMRi Q38XM0. Positions 1-915.
ModBasei Search...

Protein-protein interaction databases

STRINGi 314315.LSA0756.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAI55060 ; CAI55060 ; LCA_0756 .
GeneIDi 3778601.
KEGGi lsa:LSA0756.
PATRICi 22274332. VBILacSak116432_0733.

Phylogenomic databases

eggNOGi COG0060.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 23K.

Entry informationi

Entry nameiSYI_LACSS
AccessioniPrimary (citable) accession number: Q38XM0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 22, 2005
Last modified: May 14, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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