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Q38XM0

- SYI_LACSS

UniProt

Q38XM0 - SYI_LACSS

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Lactobacillus sakei subsp. sakei (strain 23K)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei552 – 5521Aminoacyl-adenylateUniRule annotation
Binding sitei596 – 5961ATPUniRule annotation
Metal bindingi887 – 8871ZincUniRule annotation
Metal bindingi890 – 8901ZincUniRule annotation
Metal bindingi907 – 9071ZincUniRule annotation
Metal bindingi910 – 9101ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:LCA_0756
OrganismiLactobacillus sakei subsp. sakei (strain 23K)
Taxonomic identifieri314315 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000002707: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 928928Isoleucine--tRNA ligasePRO_1000022086Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi314315.LSA0756.

Structurei

3D structure databases

ProteinModelPortaliQ38XM0.
SMRiQ38XM0. Positions 1-915.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi593 – 5975"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q38XM0-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MRIKETLNLG KTAFPMRAGL PNREIDWQKG WADNNLYQQR QKLNEGKPSF
60 70 80 90 100
VLHDGPPFAN GNIHMGHALN KTSKDIIVRY KSMNGFRAPF VPGWDTHGLP
110 120 130 140 150
IEQALAKKGI KRKEMSLVDY RKLCYDYAME QVNTQRQDFK RLGISADWDN
160 170 180 190 200
PYITLTADFE AEEIRVFGEM AKKGYIYKGK KPVYWSPSSE STLAEAEIEY
210 220 230 240 250
KDIKSPSMYV AFNVVDGKDL LDADTKFIIW TTTPWTIPAN LGIAVNPAFD
260 270 280 290 300
YVQVLADGQK YVVAAERLNK MTDLLGWESV EILKTFKGAD MELMTARHPL
310 320 330 340 350
YDRESLVILG NHVTLETGTG LVHTAPGHGE DDYNAGTKYK LPVLSVVDSK
360 370 380 390 400
GIMTEDAPGF EGVYYDKANP MVTEALEKNG SLLKLDFFTH SYPHDWRTKK
410 420 430 440 450
PVIFRATAQW FASIDAFRDQ ILAQIEKVEF MPEWGKTRLY NMIRDRGDWV
460 470 480 490 500
ISRQRAWGVP LPIFYAEDGT EIITPETIER VAQLFAEHGS NVWFEWDAKD
510 520 530 540 550
LLPAGFTHPG SPNGEFTKEK DIMDVWFDSG SSHQAVLAAR DELTYPADLI
560 570 580 590 600
LEGSDQYRGW FNSSLITSVA VGEVSPYKAV ISQGFVLDGN GRKMSKSLGN
610 620 630 640 650
TILPEKIIKQ MGADIVRLWV ASVDASSDVK VTMENFQQVS EAYRKIRNTM
660 670 680 690 700
RFMIANTTDF DPAKDTVDYA ELGSVDKFML VRLNAIIESC KAAYDAYDFA
710 720 730 740 750
TVYKTINMFL TNELSAFYLD FAKDVVYIDG QNDAPRRNMQ TVFYAVAVAL
760 770 780 790 800
TKLLTPILPH TAEEIWSYLH EPEEFVQLAE MPEVAHFAGE EDLVATWNAF
810 820 830 840 850
MGIRDDVLKA LETARMDKVI GKSLEAAVTL YPNEANAALL ASLDADVKQL
860 870 880 890 900
LIVSQLTIAD QAVEAPAEAT QFDGVAVSVA HAEGDVCDRC RMIKTDVGSD
910 920
DKFPMLCARC AAIVTANYPE AVAEGLEK
Length:928
Mass (Da):104,085
Last modified:November 22, 2005 - v1
Checksum:iB603F49C457134AC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR936503 Genomic DNA. Translation: CAI55060.1.
RefSeqiYP_395370.1. NC_007576.1.

Genome annotation databases

EnsemblBacteriaiCAI55060; CAI55060; LCA_0756.
GeneIDi3778601.
KEGGilsa:LSA0756.
PATRICi22274332. VBILacSak116432_0733.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR936503 Genomic DNA. Translation: CAI55060.1 .
RefSeqi YP_395370.1. NC_007576.1.

3D structure databases

ProteinModelPortali Q38XM0.
SMRi Q38XM0. Positions 1-915.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 314315.LSA0756.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAI55060 ; CAI55060 ; LCA_0756 .
GeneIDi 3778601.
KEGGi lsa:LSA0756.
PATRICi 22274332. VBILacSak116432_0733.

Phylogenomic databases

eggNOGi COG0060.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 23K.

Entry informationi

Entry nameiSYI_LACSS
AccessioniPrimary (citable) accession number: Q38XM0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 22, 2005
Last modified: November 26, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3