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Q38XM0 (SYI_LACSS) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase

EC=6.1.1.5
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS
Gene names
Name:ileS
Ordered Locus Names:LCA_0756
OrganismLactobacillus sakei subsp. sakei (strain 23K) [Complete proteome] [HAMAP]
Taxonomic identifier314315 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length928 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP-Rule MF_02002

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02002

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_02002

Subunit structure

Monomer By similarity. HAMAP-Rule MF_02002

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_02002.

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP-Rule MF_02002

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Metal-binding
Nucleotide-binding
Zinc
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processisoleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

isoleucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 928928Isoleucine--tRNA ligase HAMAP-Rule MF_02002
PRO_1000022086

Regions

Motif57 – 6711"HIGH" region HAMAP-Rule MF_02002
Motif593 – 5975"KMSKS" region HAMAP-Rule MF_02002

Sites

Metal binding8871Zinc By similarity
Metal binding8901Zinc By similarity
Metal binding9071Zinc By similarity
Metal binding9101Zinc By similarity
Binding site5521Aminoacyl-adenylate By similarity
Binding site5961ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q38XM0 [UniParc].

Last modified November 22, 2005. Version 1.
Checksum: B603F49C457134AC

FASTA928104,085
        10         20         30         40         50         60 
MRIKETLNLG KTAFPMRAGL PNREIDWQKG WADNNLYQQR QKLNEGKPSF VLHDGPPFAN 

        70         80         90        100        110        120 
GNIHMGHALN KTSKDIIVRY KSMNGFRAPF VPGWDTHGLP IEQALAKKGI KRKEMSLVDY 

       130        140        150        160        170        180 
RKLCYDYAME QVNTQRQDFK RLGISADWDN PYITLTADFE AEEIRVFGEM AKKGYIYKGK 

       190        200        210        220        230        240 
KPVYWSPSSE STLAEAEIEY KDIKSPSMYV AFNVVDGKDL LDADTKFIIW TTTPWTIPAN 

       250        260        270        280        290        300 
LGIAVNPAFD YVQVLADGQK YVVAAERLNK MTDLLGWESV EILKTFKGAD MELMTARHPL 

       310        320        330        340        350        360 
YDRESLVILG NHVTLETGTG LVHTAPGHGE DDYNAGTKYK LPVLSVVDSK GIMTEDAPGF 

       370        380        390        400        410        420 
EGVYYDKANP MVTEALEKNG SLLKLDFFTH SYPHDWRTKK PVIFRATAQW FASIDAFRDQ 

       430        440        450        460        470        480 
ILAQIEKVEF MPEWGKTRLY NMIRDRGDWV ISRQRAWGVP LPIFYAEDGT EIITPETIER 

       490        500        510        520        530        540 
VAQLFAEHGS NVWFEWDAKD LLPAGFTHPG SPNGEFTKEK DIMDVWFDSG SSHQAVLAAR 

       550        560        570        580        590        600 
DELTYPADLI LEGSDQYRGW FNSSLITSVA VGEVSPYKAV ISQGFVLDGN GRKMSKSLGN 

       610        620        630        640        650        660 
TILPEKIIKQ MGADIVRLWV ASVDASSDVK VTMENFQQVS EAYRKIRNTM RFMIANTTDF 

       670        680        690        700        710        720 
DPAKDTVDYA ELGSVDKFML VRLNAIIESC KAAYDAYDFA TVYKTINMFL TNELSAFYLD 

       730        740        750        760        770        780 
FAKDVVYIDG QNDAPRRNMQ TVFYAVAVAL TKLLTPILPH TAEEIWSYLH EPEEFVQLAE 

       790        800        810        820        830        840 
MPEVAHFAGE EDLVATWNAF MGIRDDVLKA LETARMDKVI GKSLEAAVTL YPNEANAALL 

       850        860        870        880        890        900 
ASLDADVKQL LIVSQLTIAD QAVEAPAEAT QFDGVAVSVA HAEGDVCDRC RMIKTDVGSD 

       910        920 
DKFPMLCARC AAIVTANYPE AVAEGLEK 

« Hide

References

[1]"The complete genome sequence of the meat-borne lactic acid bacterium Lactobacillus sakei 23K."
Chaillou S., Champomier-Verges M.-C., Cornet M., Crutz-Le Coq A.-M., Dudez A.-M., Martin V., Beaufils S., Darbon-Rongere E., Bossy R., Loux V., Zagorec M.
Nat. Biotechnol. 23:1527-1533(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 23K.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR936503 Genomic DNA. Translation: CAI55060.1.
RefSeqYP_395370.1. NC_007576.1.

3D structure databases

ProteinModelPortalQ38XM0.
SMRQ38XM0. Positions 1-915.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING314315.LSA0756.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAI55060; CAI55060; LCA_0756.
GeneID3778601.
KEGGlsa:LSA0756.
PATRIC22274332. VBILacSak116432_0733.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0060.
KOK01870.
OMAKPVHWCL.
OrthoDBEOG644ZM1.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_02002. Ile_tRNA_synth_type1.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00392. ileS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYI_LACSS
AccessionPrimary (citable) accession number: Q38XM0
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 22, 2005
Last modified: May 14, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries