Q38X32 (FTHS_LACSS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Formate--tetrahydrofolate ligase
EC=6.3.4.3

Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS

Gene names

Name:	fhs
Ordered Locus Names:	LCA_0947

Organism

Lactobacillus sakei subsp. sakei (strain 23K) [Complete proteome] [HAMAP]

Taxonomic identifier

314315 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Lactobacillaceae › Lactobacillus › Lactobacillus sakei › Lactobacillus sakei subsp. sakei

Protein attributes

Sequence length	557 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP-Rule MF_01543
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_01543
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	folic acid-containing compound biosynthetic process Inferred from electronic annotation. Source: InterPro tetrahydrofolate interconversion Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 557	557	Formate--tetrahydrofolate ligase HAMAP-Rule MF_01543		PRO_0000293042
Regions
	Nucleotide binding	67 – 74	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q38X32 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: 215009D86ECF2C63	FASTA	557	59,070
	10 20 30 40 50 60 MSDIQIAQAN EATEMKSITA IAEQIGLQAT DIEQYGPYKA KLNFQAINRL KEKEDGKLVL 70 80 90 100 110 120 VTSINPTPAG EGKSTVTVGL GDALRQLDQS AVIALREPSL GPVMGMKGGA TGGGYAQVVP 130 140 150 160 170 180 MADINLHFTG DMHALTATVN TLAALIDNHL QQGNVLNIDP RRIIWKRALD INDRALRQVV 190 200 210 220 230 240 IGLGGPVQGM PRQDGFDITV ASELMAILCL ATDITDLKNR ISKIVIGYNY DREPVTVGDL 250 260 270 280 290 300 EVTGAIAMLL KDALKPNMVQ TLEHTPALVH GGPFANIAHG CNSILATQTA LKLGDIAITE 310 320 330 340 350 360 AGFGADLGAE KFLDIKVPQL GKTPDTIVIV ATIRALKYNG GVALADLTTE NLDALKAGFS 370 380 390 400 410 420 NLAKHIANMQ RYGVPVVVSV NEFTSDTAAE VQLLQDLCQA MKVTAVPTSV WANGGQGGIE 430 440 450 460 470 480 LAKAVLAALQ QPKAFKPLYD PQADIKSKLT TVVTEIYGGR DVVFEGKAIN QLKQIEKNGW 490 500 510 520 530 540 AHLPVCIAKT QYSLSDDPKA LGAPSDFTIH VRELIPKLGA GFIVAMTGAV LTMPGLPKKP 550 AALNMDVTAD GQISGLF

References

[1]

"The complete genome sequence of the meat-borne lactic acid bacterium Lactobacillus sakei 23K." Chaillou S., Champomier-Verges M.-C., Cornet M., Crutz-Le Coq A.-M., Dudez A.-M., Martin V., Beaufils S., Darbon-Rongere E., Bossy R., Loux V., Zagorec M. Nat. Biotechnol. 23:1527-1533(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 23K.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR936503 Genomic DNA. Translation: CAI55249.1.
RefSeq	YP_395558.1. NC_007576.1.
3D structure databases
ProteinModelPortal	Q38X32.
SMR	Q38X32. Positions 3-555.
ModBase	Search...
Protein-protein interaction databases
STRING	314315.LSA0947.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAI55249; CAI55249; LCA_0947.
GeneID	3777631.
KEGG	lsa:LSA0947.
PATRIC	22274702. VBILacSak116432_0917.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2759.
KO	K01938.
OMA	EIMAVLC.
ProtClustDB	CLSK770880.
Enzyme and pathway databases
UniPathway	UPA00193.
Family and domain databases
HAMAP	MF_01543. FTHFS.
InterPro	IPR000559. Formate_THF_ligase. IPR020628. Formate_THF_ligase_CS. [Graphical view]
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
PROSITE	PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS_LACSS

Accession

Primary (citable) accession number: Q38X32

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	November 22, 2005
Last modified:	May 1, 2013
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program