Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q38WX8 (TYSY_LACSS) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thymidylate synthase

Short name=TS
Short name=TSase
EC=2.1.1.45
Gene names
Name:thyA
Ordered Locus Names:LCA_1001
OrganismLactobacillus sakei subsp. sakei (strain 23K) [Complete proteome] [HAMAP]
Taxonomic identifier314315 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length316 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP-Rule MF_00008

Catalytic activity

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP-Rule MF_00008

Pathway

Pyrimidine metabolism; dTTP biosynthesis. HAMAP-Rule MF_00008

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00008

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00008.

Sequence similarities

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
   Biological processNucleotide biosynthesis
   Cellular componentCytoplasm
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processdTMP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

dTTP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionthymidylate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 316316Thymidylate synthase HAMAP-Rule MF_00008
PRO_1000000619

Sites

Active site1981 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q38WX8 [UniParc].

Last modified November 22, 2005. Version 1.
Checksum: 3664C806C40ABFAC

FASTA31636,464
        10         20         30         40         50         60 
MSEQVYLDLA RTILEEGHYK GDRTNTGTYS LFGYQMRFNL QEGFPLLTTK KMPFGLIKSE 

        70         80         90        100        110        120 
LLWFLKGDSN IRYLLQHNNH IWDEWAFERF VKSTDYTGPD MTDFSHRAQD DADFKVVYQE 

       130        140        150        160        170        180 
QMRLFNDRIL ADEGFAKQYG ELGDIYGKQW RAWQTRSGET IDQIKNVIEM IKTNPDSRRL 

       190        200        210        220        230        240 
IVSAWNPEDV PSMALPPCHT MFQFYVNDGK LSCQLYQRSG DVFLGVPFNI ASYALLTHLI 

       250        260        270        280        290        300 
AHETGLEVGE FIHTLGDAHI YSNHVTQVKT QLARSMHAAP KLWLNPDKKS IFDFDVADIK 

       310 
VENYESEPAI KAPVAV 

« Hide

References

[1]"The complete genome sequence of the meat-borne lactic acid bacterium Lactobacillus sakei 23K."
Chaillou S., Champomier-Verges M.-C., Cornet M., Crutz-Le Coq A.-M., Dudez A.-M., Martin V., Beaufils S., Darbon-Rongere E., Bossy R., Loux V., Zagorec M.
Nat. Biotechnol. 23:1527-1533(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 23K.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR936503 Genomic DNA. Translation: CAI55303.1.
RefSeqYP_395612.1. NC_007576.1.

3D structure databases

ProteinModelPortalQ38WX8.
SMRQ38WX8. Positions 1-316.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING314315.LSA1001.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAI55303; CAI55303; LCA_1001.
GeneID3776935.
KEGGlsa:LSA1001.
PATRIC22274810. VBILacSak116432_0971.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0207.
KOK00560.
OMADVHLYSN.
OrthoDBEOG6K6V53.

Enzyme and pathway databases

UniPathwayUPA00575.

Family and domain databases

Gene3D3.30.572.10. 2 hits.
HAMAPMF_00008. Thymidy_synth_bact.
InterProIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSPR00108. THYMDSNTHASE.
SUPFAMSSF55831. SSF55831. 1 hit.
TIGRFAMsTIGR03284. thym_sym. 1 hit.
PROSITEPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTYSY_LACSS
AccessionPrimary (citable) accession number: Q38WX8
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 22, 2005
Last modified: May 14, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways