Reviewed, UniProtKB/Swiss-Prot Q38WK8 (MURA1_LACSS)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylglucosamine 1-carboxyvinyltransferase 1
    EC=2.5.1.7
Alternative name(s):
    Enoylpyruvate transferase 1
    UDP-N-acetylglucosamine enolpyruvyl transferase 1
      Short name=EPT 1

Gene names

Name:	murA1
Ordered Locus Names:	LSA1123

Organism

Lactobacillus sakei subsp. sakei (strain 23K) [Complete proteome] [HAMAP]

Taxonomic identifier

314315 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	433 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine By similarity.
Catalytic activity	Phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine. HAMAP MF_00111
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00111
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the EPSP synthase family. MurA subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	UDP-N-acetylgalactosamine biosynthetic process Inferred from electronic annotation. Source: InterPro cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 433	433	UDP-N-acetylglucosamine 1-carboxyvinyltransferase 1 HAMAP MF_00111		PRO_0000231217
Sites
	Active site	119	1	Proton donor By similarity
	Binding site	119	1	Phosphoenolpyruvate (covalent) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q38WK8-1 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: 22BAD50996169CE7	FASTA	433	46,811
	10 20 30 40 50 60 MEKLIIQGGQ PLVGDVHIEG AKNAVLPIMA AALLASKGPV ELTNVPILSD VFMMQDVLKS 70 80 90 100 110 120 LDARVKFDEQ RNYLMIDANQ PLNFEAAFEY VSKMRASIVV MGPLLARLGH ARVAMPGGCA 130 140 150 160 170 180 IGSRPVDLHL KGFEALGATI TQSHGYIEAK ADQLVGANIY LDFPSVGATQ NIMMAATLAK 190 200 210 220 230 240 GTTVIENVAR EPEIVDLANV LNKMGAKVFG AGTEEIRIEG VTELKGTEHS IVQDRIEAGT 250 260 270 280 290 300 FMIAAAATKG NVLVEEAISE HNKPLLSKLA EMGAQVIEEE NGIRIIGPDE LKPSNIKTLP 310 320 330 340 350 360 YPGFPTDMQA QMTALQLMAH GTSVMTETVF ENRFMHLEEL RRMNADYQIE GQSVILYGPP 370 380 390 400 410 420 ELTGAEVAAS DLRAAAALVI AGLVANGETL VTNLQYMDRG YYHFHQKLRA LGAHISRADF 430 DEAGHRKTTQ KLA

References

[1]

"The complete genome sequence of the meat-borne lactic acid bacterium Lactobacillus sakei 23K." Chaillou S., Champomier-Verges M.-C., Cornet M., Crutz-Le Coq A.-M., Dudez A.-M., Martin V., Beaufils S., Darbon-Rongere E., Bossy R., Loux V., Zagorec M. Nat. Biotechnol. 23:1527-1533(2005) [PubMed: 16273110] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR936503 Genomic DNA. Translation: CAI55424.1.
RefSeq	YP_395732.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q38WK8.
Genome annotation databases
GeneID	3777642.
GenomeReviews	Gene locus LSA1123 in contig CR936503_GR.
KEGG	lsa:LSA1123.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q38WK8.
OMA	MPGGCSI.
Enzyme and pathway databases
BioCyc	LSAK314315:LSA1123-MON.
Family and domain databases
HAMAP	MF_00111. [Tree]
InterPro	IPR001986. EPSP_synthase_core. IPR005750. UDP_GlcNAc_COvinyl_MurA. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 1 hit.
PANTHER	PTHR21090:SF4. AcGlu_Tran_MurA. 1 hit.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
ProDom	PD001867. EPSP_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01072. murA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURA1_LACSS

Accession

Primary (citable) accession number: Q38WK8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	November 22, 2005
Last modified:	November 3, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)