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Q38VP5 (SYL_LACSS) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:LCA_1436
OrganismLactobacillus sakei subsp. sakei (strain 23K) [Complete proteome] [HAMAP]
Taxonomic identifier314315 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length807 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 807807Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009362

Regions

Motif40 – 5112"HIGH" region HAMAP-Rule MF_00049
Motif575 – 5795"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5781ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q38VP5 [UniParc].

Last modified November 22, 2005. Version 1.
Checksum: 38344640F2DCE7B3

FASTA80792,127
        10         20         30         40         50         60 
MTYDHRAVEQ KWQAYWQSHK SFKTTEDKDK KNFYALDMFP YPSGQGLHVG HPEGYTATDI 

        70         80         90        100        110        120 
LARMKRMQGF NVLHPMGWDA FGLPAEQYAL DTGNNPADFT QKNINTFKRQ INSLGFSYDW 

       130        140        150        160        170        180 
DREVNTTDPD FYKWTQWIFE KMYEKGLAYE AEVAVNWSPD LGTVVANEEV IDGKTERGGY 

       190        200        210        220        230        240 
PVYRKPMRQW MLKITAYADR LIDDLDLVDW PESVKDMQRN WIGRSKGAEV SFAVENHDAN 

       250        260        270        280        290        300 
IDVFTTRADT MFGVSYIVMA PEHKLVADIT TPEQKAAVDA YLKEIEHKSD LERTDLAKDK 

       310        320        330        340        350        360 
TGAFTGAYAI NPVNGERLPI WISDYVLASY GTGAVMAVPA HDPRDWEFAK KFGLPLKPVV 

       370        380        390        400        410        420 
SGGNPEEAVH TEPGVMINSD FLDGLDKQAA IDKMIPWLVE HKVGHEQISY KLRDWLFSRQ 

       430        440        450        460        470        480 
RYWGEPIPII HWEDGTTSVV PEDQLPLELP LTSDIKPSGT GESPLANLTD WLNVTDENGR 

       490        500        510        520        530        540 
KGRRETNTMP QWAGSSWYYL RYIDPKNPDK LADFDKLKDW LPVDMYIGGA EHAVLHLLYV 

       550        560        570        580        590        600 
RFWHKFLYDI GVVPTKEPFQ HLYNQGMILG DNHEKMSKSK GNVVNPDDVV DRFGADTLRL 

       610        620        630        640        650        660 
YEMFMGPLDA SISWSEKGLA GARKFLDRVW RLYTEEDTDE NDQLSSKIVA DNNDQLKKVY 

       670        680        690        700        710        720 
NETVKKVTED FESMHFNVAI SQLMVFINDA YKADTFPREY AEGFVKLLAP IAPHMMEELW 

       730        740        750        760        770        780 
AMLGHDDSIS YVDWPTFDPA ALIANEVEVI FQVNGKLKAK VTVAKDTPKE ELEAMAKANE 

       790        800 
KVAEFIADKT VRKVIAIPNK LVNIVAN 

« Hide

References

[1]"The complete genome sequence of the meat-borne lactic acid bacterium Lactobacillus sakei 23K."
Chaillou S., Champomier-Verges M.-C., Cornet M., Crutz-Le Coq A.-M., Dudez A.-M., Martin V., Beaufils S., Darbon-Rongere E., Bossy R., Loux V., Zagorec M.
Nat. Biotechnol. 23:1527-1533(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 23K.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR936503 Genomic DNA. Translation: CAI55738.1.
RefSeqYP_396045.1. NC_007576.1.

3D structure databases

ProteinModelPortalQ38VP5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING314315.LSA1436.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAI55738; CAI55738; LCA_1436.
GeneID3777995.
KEGGlsa:LSA1436.
PATRIC22275678. VBILacSak116432_1400.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_LACSS
AccessionPrimary (citable) accession number: Q38VP5
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 22, 2005
Last modified: April 16, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries