Q38VA7 (NADE_LACSS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	LCA_1569

Organism

Lactobacillus sakei subsp. sakei (strain 23K) [Complete proteome] [HAMAP]

Taxonomic identifier

314315 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	275 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 275	275	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_1000077571
Regions
	Nucleotide binding	47 – 54	8	ATP By similarity
Sites
	Active site	49	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q38VA7 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: 5698345FD6DD0FEF	FASTA	275	30,282
	10 20 30 40 50 60 MNALQAEIIA ALKTQPTIDP AKEIRRSVDF MKAYLKKNTF LKSYVLGISG GQDSTLVGAL 70 80 90 100 110 120 TEKAMQEMRA ETGDESYQFI AVRLPYGEQA DESDAMAAIE FMAADQVKRV NIKGSVDAMV 130 140 150 160 170 180 QSLAETGVTV SDFNKGNIKA RVRMIAQYGI AGENSGAVLG TDHSAESITG FYTKFGDGGA 190 200 210 220 230 240 DLVPIFRLNK RQGKAMLAEL GAPKHLYEKV PTADLEEDRP ALPDELALGV TYDQIDDYLE 250 260 270 GRQVSEEAAT KIENWHKKTA HKRHLPITIY DTFWQ

References

[1]

"The complete genome sequence of the meat-borne lactic acid bacterium Lactobacillus sakei 23K." Chaillou S., Champomier-Verges M.-C., Cornet M., Crutz-Le Coq A.-M., Dudez A.-M., Martin V., Beaufils S., Darbon-Rongere E., Bossy R., Loux V., Zagorec M. Nat. Biotechnol. 23:1527-1533(2005) [PubMed: 16273110] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 23K.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR936503 Genomic DNA. Translation: CAI55876.1.
RefSeq	YP_396183.1. NC_007576.1.
3D structure databases
HSSP	HSSP built from PDB template 1WXH based on UniProtKB P18843.
ProteinModelPortal	Q38VA7.
SMR	Q38VA7. Positions 3-275.
ModBase	Search...
Protein-protein interaction databases
STRING	Q38VA7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3778149.
GenomeReviews	Gene locus LCA_1569 in contig CR936503_GR.
KEGG	lsa:LSA1569.
PATRIC	22275956. VBILacSak116432_1537.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0171.
HOGENOM	HBG351567.
OMA	IAQYEIA.
PhylomeDB	Q38VA7.
ProtClustDB	PRK00768.
Enzyme and pathway databases
BioCyc	LSAK314315:LSA1569-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_LACSS

Accession

Primary (citable) accession number: Q38VA7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	November 22, 2005
Last modified:	January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program