Q38C91 (Q38C91_TRYB2) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Methionyl-tRNA synthetase, putative EMBL EAN77579.1
EC=6.1.1.10 EMBL EAN77579.1

Gene names

ORF Names:

Tb10.70.6470 EMBL EAN77579.1

Organism

Trypanosoma brucei brucei (strain 927/4 GUTat10.1) [Reference proteome]

Taxonomic identifier

999953 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Euglenozoa › Kinetoplastida › Trypanosomatidae › Trypanosoma › Trypanozoon › Trypanosoma brucei › Trypanosoma brucei brucei

Protein attributes

Sequence length	773 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Molecular function	Aminoacyl-tRNA synthetase EMBL EAN77579.1 Ligase
Technical term	3D-structure PDB 3TUN PDB 4EG1 PDB 4EG3 PDB 4EG4 PDB 4EG5 PDB 4EG6 PDB 4EG7 PDB 4EG8 PDB 4EGA PDB 3U1E PDB 3U1F PDB 3U1G PDB 3U1Z PDB 3U20 Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: InterPro methionine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q38C91 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: 8BE9FB1CFB52A1B1	FASTA	773	86,911
	10 20 30 40 50 60 MALKLLSEKA NSQALKVLLC SYYVKRPVEV SLSGAYATPI LHHPAFKQPI IAPNEMARVI 70 80 90 100 110 120 LFYSVEPTSN NGGAADSSNG DGTASPVAGL TNLTLEHETW LEWEATTFTR AVHPLYTQRR 130 140 150 160 170 180 QTAESLAVFS YLDKKISEND DRCVYSPAVE GKGAADPTDA VSTFFIDCIV WCAVLPALCE 190 200 210 220 230 240 SGVLRDSEKQ QLPHLVKWFN TFQKEQKTLI DNAFENLSVQ EAADFLRCPR VYKVSAKVEK 250 260 270 280 290 300 VFFVTSPIYY VNAAPHIGHV YSTLITDVIG RYHRVKGERV FALTGTDEHG QKVAEAAKQK 310 320 330 340 350 360 QVSPYDFTAA VAGEFKKCFE QMDYSIDYFI RTTNEQHKAV VKELWTKLEQ KGDIYLGRYE 370 380 390 400 410 420 GWYSISDESF LTPQNITDGV DKDGNPCKVS LESGHVVTWV SEENYMFRLS AFRERLLEWY 430 440 450 460 470 480 HANPGCIVPE FRRREVIRAV EKGLPDLSVS RKKETLHNWA IPVPGNPDHC VYVWLDALTN 490 500 510 520 530 540 YLTGSRLRVD ESGKEVSLAD DFSELERFPA DVHVIGKDIL KFHAIYWPAF LLSAGLPLPK 550 560 570 580 590 600 KIVAHGWWTK DRKKISKSLG NVFDPVEKAE EFGYDALKYF LLRESGFSDD GDYSDKNMIA 610 620 630 640 650 660 RLNGELADTL GNLVMRCTSA KINVNGEWPS PAAYTEEDES LIQLIKDLPG TADHYYLIPD 670 680 690 700 710 720 IQKAIIAVFD VLRAINAYVT DMAPWKLVKT DPERLRTVLY ITLEGVRVTT LLLSPILPRK 730 740 750 760 770 SVVIFDMLGV PEVHRKGIEN FEFGAVPPGT RLGPAVEGEV LFSKRSTENT KST

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome of the African trypanosome Trypanosoma brucei." Berriman M., Ghedin E., Hertz-Fowler C., Blandin G., Renauld H., Bartholomeu D.C., Lennard N.J., Caler E., Hamlin N.E., Haas B., Bohme U., Hannick L., Aslett M.A., Shallom J., Marcello L., Hou L., Wickstead B., Alsmark U.C.M. , Arrowsmith C., Atkin R.J., Barron A.J., Bringaud F., Brooks K., Carrington M., Cherevach I., Chillingworth T.J., Churcher C., Clark L.N., Corton C.H., Cronin A., Davies R.M., Doggett J., Djikeng A., Feldblyum T., Field M.C., Fraser A., Goodhead I., Hance Z., Harper D., Harris B.R., Hauser H., Hostetler J., Ivens A., Jagels K., Johnson D., Johnson J., Jones K., Kerhornou A.X., Koo H., Larke N., Landfear S., Larkin C., Leech V., Line A., Lord A., Macleod A., Mooney P.J., Moule S., Martin D.M., Morgan G.W., Mungall K., Norbertczak H., Ormond D., Pai G., Peacock C.S., Peterson J., Quail M.A., Rabbinowitsch E., Rajandream M.A., Reitter C., Salzberg S.L., Sanders M., Schobel S., Sharp S., Simmonds M., Simpson A.J., Tallon L., Turner C.M., Tait A., Tivey A.R., Van Aken S., Walker D., Wanless D., Wang S., White B., White O., Whitehead S., Woodward J., Wortman J., Adams M.D., Embley T.M., Gull K., Ullu E., Barry J.D., Fairlamb A.H., Opperdoes F., Barrell B.G., Donelson J.E., Hall N., Fraser C.M., Melville S.E., El-Sayed N.M.A. Science 309:416-422(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 927/4 GUTat10.1 EMBL EAN77579.1.
[2]	"Urea-based Inhibitors of Trypanosoma brucei Methionyl-tRNA Synthetase Show Promising Selectivity and Bioavailability." Shibata S., Gillespie J.R., Pefley R.M., Koh C.Y., Kim J.E., Laydbak J.U., Hulverson M.A., Zucker F., Hol W.G.J., Verlinde C.L.M.J., Buckner F.S., Fan E. Submitted (SEP-2011) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.31 ANGSTROMS) OF 237-773.
[3]	"Urea-based inhibitors of Trypanosoma brucei methionyl-tRNA synthetase: selectivity and in vivo characterization." Shibata S., Gillespie J.R., Ranade R.M., Koh C.Y., Kim J.E., Laydbak J.U., Zucker F.H., Hol W.G., Verlinde C.L., Buckner F.S., Fan E. J. Med. Chem. 55:6342-6351(2012) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.55 ANGSTROMS) OF 237-773.
[4]	"Distinct states of methionyl-tRNA synthetase indicate inhibitor binding by conformational selection." Koh C.Y., Kim J.E., Shibata S., Ranade R.M., Yu M., Liu J., Gillespie J.R., Buckner F.S., Verlinde C.L., Fan E., Hol W.G. Structure 20:1681-1691(2012) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS) OF 237-773.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

CM000208 Genomic DNA. Translation: EAN77579.1.

RefSeq

XP_822407.1. XM_817314.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3TUN	X-ray	2.55	A/B	237-773	[»]
3U1E	X-ray	2.31	A/B	237-773	[»]
3U1F	X-ray	2.20	A/B	237-773	[»]
3U1G	X-ray	2.35	A/B	237-773	[»]
3U1Z	X-ray	2.90	A/B	237-773	[»]
3U20	X-ray	2.49	A/B	237-773	[»]
4EG1	X-ray	2.90	A/B	237-773	[»]
4EG3	X-ray	2.94	A/B	237-773	[»]
4EG4	X-ray	3.15	A/B	237-773	[»]
4EG5	X-ray	3.10	A/B	237-773	[»]
4EG6	X-ray	2.90	A/B	237-773	[»]
4EG7	X-ray	2.75	A/B	237-773	[»]
4EG8	X-ray	2.60	A/B	237-773	[»]
4EGA	X-ray	2.70	A/B	237-773	[»]

ProteinModelPortal

Q38C91.

ModBase

Search...

Protein-protein interaction databases

STRING

5691.Tb10.70.6470.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblProtists

EAN77579; EAN77579; Tb10.70.6470.

GeneID

3662741.

KEGG

tbr:Tb10.70.6470.

Phylogenomic databases

eggNOG

COG0143.

HOGENOM

HOG000200401.

KO

K01874.

OMA

IMAKEIS.

ProtClustDB

CLSZ2440184.

Family and domain databases

Gene3D

3.40.50.620. 2 hits.

InterPro

IPR014758. Met-tRNA_synth.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]

Pfam

PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]

PRINTS

PR01041. TRNASYNTHMET.

SUPFAM

SSF47323. tRNAsyn_1a_bind. 1 hit.

TIGRFAMs

TIGR00398. metG. 1 hit.

ProtoNet

Search...

Entry information

Entry name

Q38C91_TRYB2

Accession

Primary (citable) accession number: Q38C91

Entry history

Integrated into UniProtKB/TrEMBL:	November 22, 2005
Last sequence update:	November 22, 2005
Last modified:	May 1, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)