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Protein

Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1

Gene

MTR1

Organism
Trypanosoma brucei brucei (strain 927/4 GUTat10.1)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

S-adenosyl-L-methionine-dependent methyltransferase that mediates RNA cap1 2'-O-ribose methylation to the 5'-cap structure of spliced leader and U1 small nuclear RNAs. Methylates the ribose of the first nucleotide of a m7GpppG-capped RNA to produce m7GpppNmp (cap1). Cap1 modification is linked to higher levels of translation. Recognizes a guanosine cap on RNA independent of its N7 methylation status.2 Publications

Catalytic activityi

S-adenosyl-L-methionine + a 5'-(N7-methyl 5'-triphosphoguanosine)-(purine-ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-(2'-O-methyl-purine-ribonucleotide)-[mRNA].1 Publication

Kineticsi

  1. KM=0.8 µM for S-adenosyl-L-methionine1 Publication

    pH dependencei

    Optimum pH is 9.5.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei130S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotation1
    Binding sitei207S-adenosyl-L-methionineCurated1
    Active sitei248Proton acceptorCurated1

    GO - Molecular functioni

    GO - Biological processi

    • 7-methylguanosine mRNA capping Source: UniProtKB
    • cap1 mRNA methylation Source: UniProtKB
    • mRNA methylation Source: UniProtKB
    • RNA methylation Source: GeneDB

    Keywordsi

    Molecular functionMethyltransferase, Transferase
    Biological processmRNA capping, mRNA processing
    LigandS-adenosyl-L-methionine

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1 (EC:2.1.1.57)
    Alternative name(s):
    Cap1 2'O-ribose methyltransferase 1
    Short name:
    MTr1
    Short name:
    TbMTr1
    Gene namesi
    Name:MTR1
    ORF Names:Tb10.6k15.2610
    OrganismiTrypanosoma brucei brucei (strain 927/4 GUTat10.1)
    Taxonomic identifieri185431 [NCBI]
    Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosoma
    Proteomesi
    • UP000008524 Componenti: Chromosome 10

    Organism-specific databases

    EuPathDBiTriTrypDB:Tb927.10.7940

    Subcellular locationi

    GO - Cellular componenti

    • nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi95K → A: 98% reduction of activity. 1 Publication1
    Mutagenesisi207D → A: 96% reduction of activity. 1 Publication1
    Mutagenesisi248K → A: 98% reduction of activity. 1 Publication1
    Mutagenesisi285E → A: 93% reduction of activity. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00003998021 – 370Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1Add BLAST370

    Proteomic databases

    PaxDbiQ38AH0

    Interactioni

    Subunit structurei

    Component of a complex composed of CBF5, GAR1, NHP2, MTR1, NOP10 and Tb11.01.8210.1 Publication

    Protein-protein interaction databases

    STRINGi5691.EAN78200

    Structurei

    3D structure databases

    ProteinModelPortaliQ38AH0
    SMRiQ38AH0
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini87 – 294RrmJ-type SAM-dependent 2'-O-MTasePROSITE-ProRule annotationAdd BLAST208

    Phylogenomic databases

    HOGENOMiHOG000130918
    KOiK14589
    OMAiMESTGVW

    Family and domain databases

    InterProiView protein in InterPro
    IPR030376 Cap_mRNA_MeTrfase_1
    IPR025816 RrmJ-type_MeTrfase
    IPR002877 rRNA_MeTrfase_FtsJ_dom
    IPR029063 SAM-dependent_MTases
    PANTHERiPTHR16121:SF0 PTHR16121:SF0, 1 hit
    PfamiView protein in Pfam
    PF01728 FtsJ, 1 hit
    SUPFAMiSSF53335 SSF53335, 1 hit
    PROSITEiView protein in PROSITE
    PS51613 SAM_MT_RRMJ, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q38AH0-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MPAVADCTVV FPLRHDPAST HPDVSGLVGK AFERVNWRDA FDTFLAEERS
    60 70 80 90 100
    ALWAAKTAFD NTDTSAYIAA RDALFPQAVS GVHGAVAFRN RAGHKLHETM
    110 120 130 140 150
    EAVGLWEYLK GGATRAKGTF TFVDVCGGPG AFSQALFAMG KEHKLRLRGF
    160 170 180 190 200
    GLTLRNVKGL DWYTDLPSRS FFPCYGIDGT GDVFKLENIE SLCSLTCKEN
    210 220 230 240 250
    VRLVVADGGF DVPTEVVNFQ ETISCRIVYG QWLSAVKLLR PGGCFVLKLF
    260 270 280 290 300
    DCFSPFTRAI LFLTTHLYES VQVVKPRHSR VVNSERYLVC IGFIGAPKQW
    310 320 330 340 350
    LEHFERCYQE GFVDNDNIPT VLPTSLFSGD KIFGADVERM SATIASNQVS
    360 370
    GLHAILEKLQ SKPAMEEVKS
    Length:370
    Mass (Da):40,896
    Last modified:November 22, 2005 - v1
    Checksum:i1BC008132428795F
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CM000208 Genomic DNA Translation: EAN78200.1
    RefSeqiXP_823028.1, XM_817935.1

    Genome annotation databases

    GeneDBiTb927.10.7940:pep
    GeneIDi3662615
    KEGGitbr:Tb10.6k15.2610

    Similar proteinsi

    Entry informationi

    Entry nameiMTR1_TRYB2
    AccessioniPrimary (citable) accession number: Q38AH0
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 5, 2010
    Last sequence update: November 22, 2005
    Last modified: April 25, 2018
    This is version 66 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

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