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Protein

Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1

Gene

MTR1

Organism
Trypanosoma brucei brucei (strain 927/4 GUTat10.1)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

S-adenosyl-L-methionine-dependent methyltransferase that mediates RNA cap1 2'-O-ribose methylation to the 5'-cap structure of spliced leader and U1 small nuclear RNAs. Methylates the ribose of the first nucleotide of a m7GpppG-capped RNA to produce m7GpppNmp (cap1). Cap1 modification is linked to higher levels of translation. Recognizes a guanosine cap on RNA independent of its N7 methylation status.2 Publications

Catalytic activityi

S-adenosyl-L-methionine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(purine-ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-purine-ribonucleotide)-[mRNA].1 Publication

Kineticsi

  1. KM=0.8 µM for S-adenosyl-L-methionine1 Publication

    pH dependencei

    Optimum pH is 9.5.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei130 – 1301S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotation
    Binding sitei207 – 2071S-adenosyl-L-methionineCurated
    Active sitei248 – 2481Proton acceptorCurated

    GO - Molecular functioni

    GO - Biological processi

    • 7-methylguanosine mRNA capping Source: UniProtKB
    • cap1 mRNA methylation Source: UniProtKB
    • mRNA methylation Source: UniProtKB
    • RNA methylation Source: GeneDB
    • rRNA methylation Source: GOC
    Complete GO annotation...

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    mRNA capping, mRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1 (EC:2.1.1.57)
    Alternative name(s):
    Cap1 2'O-ribose methyltransferase 1
    Short name:
    MTr1
    Short name:
    TbMTr1
    Gene namesi
    Name:MTR1
    ORF Names:Tb10.6k15.2610
    OrganismiTrypanosoma brucei brucei (strain 927/4 GUTat10.1)
    Taxonomic identifieri185431 [NCBI]
    Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosoma
    Proteomesi
    • UP000008524 Componenti: Chromosome 10

    Organism-specific databases

    EuPathDBiTriTrypDB:Tb927.10.7940.

    Subcellular locationi

    GO - Cellular componenti

    • nucleus Source: UniProtKB
    Complete GO annotation...

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi95 – 951K → A: 98% reduction of activity. 1 Publication
    Mutagenesisi207 – 2071D → A: 96% reduction of activity. 1 Publication
    Mutagenesisi248 – 2481K → A: 98% reduction of activity. 1 Publication
    Mutagenesisi285 – 2851E → A: 93% reduction of activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 370370Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1PRO_0000399802Add
    BLAST

    Interactioni

    Subunit structurei

    Component of a complex composed of CBF5, GAR1, NHP2, MTR1, NOP10 and Tb11.01.8210.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ38AH0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini87 – 294208RrmJ-type SAM-dependent 2'-O-MTasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 RrmJ-type SAM-dependent 2'-O-MTase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    HOGENOMiHOG000130918.
    KOiK14589.
    OMAiNRAGHKL.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR025816. RrmJ-type_MeTrfase.
    IPR002877. rRNA_MeTrfase_FtsJ_dom.
    IPR029063. SAM-dependent_MTases.
    [Graphical view]
    PfamiPF01728. FtsJ. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS51613. SAM_MT_RRMJ. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q38AH0-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MPAVADCTVV FPLRHDPAST HPDVSGLVGK AFERVNWRDA FDTFLAEERS
    60 70 80 90 100
    ALWAAKTAFD NTDTSAYIAA RDALFPQAVS GVHGAVAFRN RAGHKLHETM
    110 120 130 140 150
    EAVGLWEYLK GGATRAKGTF TFVDVCGGPG AFSQALFAMG KEHKLRLRGF
    160 170 180 190 200
    GLTLRNVKGL DWYTDLPSRS FFPCYGIDGT GDVFKLENIE SLCSLTCKEN
    210 220 230 240 250
    VRLVVADGGF DVPTEVVNFQ ETISCRIVYG QWLSAVKLLR PGGCFVLKLF
    260 270 280 290 300
    DCFSPFTRAI LFLTTHLYES VQVVKPRHSR VVNSERYLVC IGFIGAPKQW
    310 320 330 340 350
    LEHFERCYQE GFVDNDNIPT VLPTSLFSGD KIFGADVERM SATIASNQVS
    360 370
    GLHAILEKLQ SKPAMEEVKS
    Length:370
    Mass (Da):40,896
    Last modified:November 22, 2005 - v1
    Checksum:i1BC008132428795F
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CM000208 Genomic DNA. Translation: EAN78200.1.
    RefSeqiXP_823028.1. XM_817935.1.

    Genome annotation databases

    GeneDBiTb927.10.7940:pep.
    GeneIDi3662615.
    KEGGitbr:Tb10.6k15.2610.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CM000208 Genomic DNA. Translation: EAN78200.1.
    RefSeqiXP_823028.1. XM_817935.1.

    3D structure databases

    ProteinModelPortaliQ38AH0.
    ModBaseiSearch...
    MobiDBiSearch...

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    GeneDBiTb927.10.7940:pep.
    GeneIDi3662615.
    KEGGitbr:Tb10.6k15.2610.

    Organism-specific databases

    EuPathDBiTriTrypDB:Tb927.10.7940.

    Phylogenomic databases

    HOGENOMiHOG000130918.
    KOiK14589.
    OMAiNRAGHKL.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR025816. RrmJ-type_MeTrfase.
    IPR002877. rRNA_MeTrfase_FtsJ_dom.
    IPR029063. SAM-dependent_MTases.
    [Graphical view]
    PfamiPF01728. FtsJ. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS51613. SAM_MT_RRMJ. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "The genome of the African trypanosome Trypanosoma brucei."
      Berriman M., Ghedin E., Hertz-Fowler C., Blandin G., Renauld H., Bartholomeu D.C., Lennard N.J., Caler E., Hamlin N.E., Haas B., Bohme U., Hannick L., Aslett M.A., Shallom J., Marcello L., Hou L., Wickstead B., Alsmark U.C.M.
      , Arrowsmith C., Atkin R.J., Barron A.J., Bringaud F., Brooks K., Carrington M., Cherevach I., Chillingworth T.J., Churcher C., Clark L.N., Corton C.H., Cronin A., Davies R.M., Doggett J., Djikeng A., Feldblyum T., Field M.C., Fraser A., Goodhead I., Hance Z., Harper D., Harris B.R., Hauser H., Hostetler J., Ivens A., Jagels K., Johnson D., Johnson J., Jones K., Kerhornou A.X., Koo H., Larke N., Landfear S., Larkin C., Leech V., Line A., Lord A., Macleod A., Mooney P.J., Moule S., Martin D.M., Morgan G.W., Mungall K., Norbertczak H., Ormond D., Pai G., Peacock C.S., Peterson J., Quail M.A., Rabbinowitsch E., Rajandream M.A., Reitter C., Salzberg S.L., Sanders M., Schobel S., Sharp S., Simmonds M., Simpson A.J., Tallon L., Turner C.M., Tait A., Tivey A.R., Van Aken S., Walker D., Wanless D., Wang S., White B., White O., Whitehead S., Woodward J., Wortman J., Adams M.D., Embley T.M., Gull K., Ullu E., Barry J.D., Fairlamb A.H., Opperdoes F., Barrell B.G., Donelson J.E., Hall N., Fraser C.M., Melville S.E., El-Sayed N.M.A.
      Science 309:416-422(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 927/4 GUTat10.1Imported.
    2. "The 2'-O-ribose methyltransferase for cap 1 of spliced leader RNA and U1 small nuclear RNA in Trypanosoma brucei."
      Zamudio J.R., Mittra B., Foldynova-Trantirkova S., Zeiner G.M., Lukes J., Bujnicki J.M., Sturm N.R., Campbell D.A.
      Mol. Cell. Biol. 27:6084-6092(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION.
    3. "The TbMTr1 spliced leader RNA cap 1 2'-O-ribose methyltransferase from Trypanosoma brucei acts with substrate specificity."
      Mittra B., Zamudio J.R., Bujnicki J.M., Stepinski J., Darzynkiewicz E., Campbell D.A., Sturm N.R.
      J. Biol. Chem. 283:3161-3172(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF LYS-95; ASP-207; LYS-248 AND GLU-285.
      Strain: YTAT.
    4. "Trypanosoma brucei spliced leader RNA maturation by the cap 1 2'-O-ribose methyltransferase and SLA1 H/ACA snoRNA pseudouridine synthase complex."
      Zamudio J.R., Mittra B., Chattopadhyay A., Wohlschlegel J.A., Sturm N.R., Campbell D.A.
      Mol. Cell. Biol. 29:1202-1211(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT.
      Strain: YTAT.

    Entry informationi

    Entry nameiMTR1_TRYB2
    AccessioniPrimary (citable) accession number: Q38AH0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 5, 2010
    Last sequence update: November 22, 2005
    Last modified: March 16, 2016
    This is version 54 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.