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Q38998

- AKT1_ARATH

UniProt

Q38998 - AKT1_ARATH

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Protein

Potassium channel AKT1

Gene

AKT1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Highly selective inward-rectifying potassium channel that mediate potassium uptake by plant roots in response to low K+ conditions, by a calcium-, CBL-, and CIPK-dependent pathway. Positively regulated by phosphorylation by CIPK23. Negatively regulated by a kinase-independent regulatory mechanism involving a competing direct binding of CBL10. Involved in the stomatal regulation by monitoring the turgor pressure in guard cells. Assuming opened or closed conformations in response to the voltage difference across the membrane, the channel is activated by hyperpolarization. May interact with the cytoskeleton or with regulatory proteins.5 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi372 – 493122cNMPAdd
BLAST

GO - Molecular functioni

  1. identical protein binding Source: IntAct
  2. inward rectifier potassium channel activity Source: TAIR

GO - Biological processi

  1. potassium ion import Source: TAIR
  2. potassium ion transport Source: TAIR
  3. regulation of stomatal closure Source: TAIR
  4. response to salt stress Source: TAIR
  5. response to water deprivation Source: TAIR
  6. root hair elongation Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Potassium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

BioCyciARA:AT2G26550-MONOMER.
MetaCyc:MONOMER-14552.

Protein family/group databases

TCDBi1.A.1.4.1. the voltage-gated ion channel (vic) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium channel AKT1
Gene namesi
Name:AKT1
Ordered Locus Names:At2g26650
ORF Names:F18A8.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G26650.

Subcellular locationi

Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 6161CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei62 – 8221Helical; Name=Segment S1Sequence AnalysisAdd
BLAST
Topological domaini83 – 908ExtracellularSequence Analysis
Transmembranei91 – 11121Helical; Name=Segment S2Sequence AnalysisAdd
BLAST
Topological domaini112 – 13423CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei135 – 15521Helical; Name=Segment S3Sequence AnalysisAdd
BLAST
Topological domaini156 – 1583ExtracellularSequence Analysis
Transmembranei159 – 17921Helical; Voltage-sensor; Name=Segment S4Sequence AnalysisAdd
BLAST
Topological domaini180 – 19314CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei194 – 21421Helical; Name=Segment S5Sequence AnalysisAdd
BLAST
Topological domaini215 – 24127ExtracellularSequence AnalysisAdd
BLAST
Intramembranei242 – 26120Pore-forming; Name=Segment H5Sequence AnalysisAdd
BLAST
Topological domaini262 – 2654ExtracellularSequence Analysis
Transmembranei266 – 28621Helical; Name=Segment S6Sequence AnalysisAdd
BLAST
Topological domaini287 – 857571CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 857857Potassium channel AKT1PRO_0000054121Add
BLAST

Post-translational modificationi

Phosphorylated by CIPK proteins CIPK6, CIPK16 and CIPK23. The activation by phosphorylation is induced by low K+ conditions and stimulates K+ uptake and relocation. Dephosphorylation by AIP1 repressed the transport activity.3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ38998.
PRIDEiQ38998.

Expressioni

Tissue specificityi

Preferentially expressed in the peripheral cell layers of root mature including root cortex and root hairs. Detected also, at a lower level, in the mesophyll of the leaves and at restricted sites corresponding to hydathodes and guard cells.2 Publications

Inductioni

In roots, strongly reduced after 2,4-dichlorophenoxyacetic acid (2,4-D) treatment and weakly reduced after benzyladenine (BA) treatment. In shoots, strongly reduced after abscisic acid (ABA) treatment and induced after benzyladenine (BA) treatment.1 Publication

Gene expression databases

ExpressionAtlasiQ38998. baseline and differential.
GenevestigatoriQ38998.

Interactioni

Subunit structurei

The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming subunits. Possible heteromultimer with AKT2 or KAT3. Part of a K+-channel calcium-sensing kinase/phosphatase complex composed by a calcium sensor CBL (CBL1, CBL2, CBL3 or CBL9), a kinase CIPK (CIPK6, CIPK16 or CIPK23), a phosphatase PP2C (AIP1) and a K+-channel (AKT1). Interacts directly with AIP1, CBL10, CIPK6, CIPK16 and CIPK23.6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself7EBI-974289,EBI-974289
AKT2Q388983EBI-974289,EBI-1552774
CIPK23Q93VD34EBI-974289,EBI-974277

Protein-protein interaction databases

BioGridi2558. 12 interactions.
DIPiDIP-36762N.
IntActiQ38998. 7 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ38998.
SMRiQ38998. Positions 190-299, 381-702.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati515 – 54632ANK 1Add
BLAST
Repeati550 – 57930ANK 2Add
BLAST
Repeati583 – 61230ANK 3Add
BLAST
Repeati614 – 64330ANK 4Add
BLAST
Repeati647 – 67630ANK 5Add
BLAST
Repeati680 – 70930ANK 6Add
BLAST
Domaini790 – 85768KHAPROSITE-ProRule annotationAdd
BLAST

Domaini

The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids. The pore-forming region H5 is enclosed by the transmembrane segments S5 and S6 in the Shaker-type (1P/6TM) and contains the GYGD signature motif which seems to be involved in potassium selectivity.
The KHA domain (rich in hydrophobic and acidic residues) present in the C-terminal part is likely to be important for tetramerization.

Sequence similaritiesi

Contains 6 ANK repeats.PROSITE-ProRule annotation
Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation
Contains 1 KHA domain.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0666.
HOGENOMiHOG000238230.
InParanoidiQ38998.
OMAiAEQQGHE.
PhylomeDBiQ38998.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
2.60.120.10. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR021789. K_channel_plant.
IPR003938. K_chnl_volt-dep_EAG/ELK/ERG.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00023. Ank. 3 hits.
PF00027. cNMP_binding. 1 hit.
PF11834. DUF3354. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
PR01463. EAGCHANLFMLY.
SMARTiSM00248. ANK. 5 hits.
SM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF51206. SSF51206. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 3 hits.
PS50042. CNMP_BINDING_3. 1 hit.
PS51490. KHA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q38998-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRGGALLCGQ VQDEIEQLSR ESSHFSLSTG ILPSLGARSN RRVKLRRFVV
60 70 80 90 100
SPYDHKYRIW EAFLVVLVVY TAWVSPFEFG FLRKPRPPLS ITDNIVNAFF
110 120 130 140 150
AIDIIMTFFV GYLDKSTYLI VDDRKQIAFK YLRSWFLLDL VSTIPSEAAM
160 170 180 190 200
RISSQSYGLF NMLRLWRLRR VGALFARLEK DRNFNYFWVR CAKLVCVTLF
210 220 230 240 250
AVHCAACFYY LIAARNSNPA KTWIGANVAN FLEESLWMRY VTSMYWSITT
260 270 280 290 300
LTTVGYGDLH PVNTKEMIFD IFYMLFNLGL TAYLIGNMTN LVVHGTSRTR
310 320 330 340 350
NFRDTIQAAS NFAHRNHLPP RLQDQMLAHL CLKYRTDSEG LQQQETLDAL
360 370 380 390 400
PKAIRSSISH FLFYSLMDKV YLFRGVSNDL LFQLVSEMKA EYFPPKEDVI
410 420 430 440 450
LQNEAPTDFY ILVNGTADLV DVDTGTESIV REVKAGDIIG EIGVLCYRPQ
460 470 480 490 500
LFTVRTKRLC QLLRMNRTTF LNIIQANVGD GTIIMNNLLQ HLKEMNDPVM
510 520 530 540 550
TNVLLEIENM LARGKMDLPL NLCFAAIRED DLLLHQLLKR GLDPNESDNN
560 570 580 590 600
GRTPLHIAAS KGTLNCVLLL LEYHADPNCR DAEGSVPLWE AMVEGHEKVV
610 620 630 640 650
KVLLEHGSTI DAGDVGHFAC TAAEQGNLKL LKEIVLHGGD VTRPRATGTS
660 670 680 690 700
ALHTAVCEEN IEMVKYLLEQ GADVNKQDMH GWTPRDLAEQ QGHEDIKALF
710 720 730 740 750
REKLHERRVH IETSSSVPIL KTGIRFLGRF TSEPNIRPAS REVSFRIRET
760 770 780 790 800
RARRKTNNFD NSLFGILANQ SVPKNGLATV DEGRTGNPVR VTISCAEKDD
810 820 830 840 850
IAGKLVLLPG SFKELLELGS NKFGIVATKV MNKDNNAEID DVDVIRDGDH

LIFATDS
Length:857
Mass (Da):96,990
Last modified:January 1, 1998 - v2
Checksum:i28E1622BA3505F4C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62907 mRNA. Translation: CAA44693.1.
U06745 Genomic DNA. Translation: AAA96810.1.
AC003105 Genomic DNA. Translation: AAB95299.1.
CP002685 Genomic DNA. Translation: AEC07870.1.
AK227601 mRNA. Translation: BAE99592.1.
BT006442 mRNA. Translation: AAP21250.1.
PIRiS23606.
S62694.
RefSeqiNP_180233.1. NM_128222.5.
UniGeneiAt.48521.
At.68413.

Genome annotation databases

EnsemblPlantsiAT2G26650.1; AT2G26650.1; AT2G26650.
GeneIDi817206.
KEGGiath:AT2G26650.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62907 mRNA. Translation: CAA44693.1 .
U06745 Genomic DNA. Translation: AAA96810.1 .
AC003105 Genomic DNA. Translation: AAB95299.1 .
CP002685 Genomic DNA. Translation: AEC07870.1 .
AK227601 mRNA. Translation: BAE99592.1 .
BT006442 mRNA. Translation: AAP21250.1 .
PIRi S23606.
S62694.
RefSeqi NP_180233.1. NM_128222.5.
UniGenei At.48521.
At.68413.

3D structure databases

ProteinModelPortali Q38998.
SMRi Q38998. Positions 190-299, 381-702.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 2558. 12 interactions.
DIPi DIP-36762N.
IntActi Q38998. 7 interactions.

Protein family/group databases

TCDBi 1.A.1.4.1. the voltage-gated ion channel (vic) superfamily.

Proteomic databases

PaxDbi Q38998.
PRIDEi Q38998.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G26650.1 ; AT2G26650.1 ; AT2G26650 .
GeneIDi 817206.
KEGGi ath:AT2G26650.

Organism-specific databases

GeneFarmi 2792. 266.
TAIRi AT2G26650.

Phylogenomic databases

eggNOGi COG0666.
HOGENOMi HOG000238230.
InParanoidi Q38998.
OMAi AEQQGHE.
PhylomeDBi Q38998.

Enzyme and pathway databases

BioCyci ARA:AT2G26550-MONOMER.
MetaCyc:MONOMER-14552.

Gene expression databases

ExpressionAtlasi Q38998. baseline and differential.
Genevestigatori Q38998.

Family and domain databases

Gene3Di 1.25.40.20. 1 hit.
2.60.120.10. 1 hit.
InterProi IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR021789. K_channel_plant.
IPR003938. K_chnl_volt-dep_EAG/ELK/ERG.
IPR014710. RmlC-like_jellyroll.
[Graphical view ]
Pfami PF00023. Ank. 3 hits.
PF00027. cNMP_binding. 1 hit.
PF11834. DUF3354. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view ]
PRINTSi PR01415. ANKYRIN.
PR01463. EAGCHANLFMLY.
SMARTi SM00248. ANK. 5 hits.
SM00100. cNMP. 1 hit.
[Graphical view ]
SUPFAMi SSF48403. SSF48403. 1 hit.
SSF51206. SSF51206. 1 hit.
PROSITEi PS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 3 hits.
PS50042. CNMP_BINDING_3. 1 hit.
PS51490. KHA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and expression in yeast of a plant potassium ion transport system."
    Sentenac H., Bonneaud N., Minet M., Lacroute F., Salmon J.-M., Gaymard F., Grignon C.
    Science 256:663-665(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Landsberg erecta.
  2. Sentenac H.
    Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. "Organization and expression of the gene coding for the potassium transport system AKT1 of Arabidopsis thaliana."
    Basset M., Conejero G., Lepetit M., Fourcroy P., Sentenac H.
    Plant Mol. Biol. 29:947-958(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
    Strain: cv. Landsberg erecta.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  5. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  6. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 79-857.
    Strain: cv. Columbia.
  7. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 106-857.
    Strain: cv. Columbia.
  8. "Tissue-specific expression of Arabidopsis AKT1 gene is consistent with a role in K+ nutrition."
    Lagarde D., Basset M., Lepetit M., Conejero G., Gaymard F., Astruc S., Grignon C.
    Plant J. 9:195-203(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  9. "Tetramerization of the AKT1 plant potassium channel involves its C-terminal cytoplasmic domain."
    Daram P., Urbach S., Gaymard F., Sentenac H., Cherel I.
    EMBO J. 16:3455-3463(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
  10. "A role for the AKT1 potassium channel in plant nutrition."
    Hirsch R.E., Lewis B.D., Spalding E.P., Sussman M.R.
    Science 280:918-921(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. Cited for: GENE FAMILY, NOMENCLATURE.
  12. "Regulated expression of Arabidopsis shaker K(+) channel genes involved in K(+) uptake and distribution in the plant."
    Pilot G., Gaymard F., Mouline K., Cherel I., Sentenac H.
    Plant Mol. Biol. 51:773-787(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH AKT2 AND KAT3, INDUCTION.
  13. "A protein kinase, interacting with two calcineurin B-like proteins, regulates K+ transporter AKT1 in Arabidopsis."
    Xu J., Li H.-D., Chen L.-Q., Wang Y., Liu L.-L., He L., Wu W.-H.
    Cell 125:1347-1360(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CIPK23, PHOSPHORYLATION, SUBCELLULAR LOCATION.
  14. "A Ca(2)+ signaling pathway regulates a K(+) channel for low-K response in Arabidopsis."
    Li L., Kim B.-G., Cheong Y.H., Pandey G.K., Luan S.
    Proc. Natl. Acad. Sci. U.S.A. 103:12625-12630(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CIPK23, PHOSPHORYLATION.
  15. "A protein phosphorylation/dephosphorylation network regulates a plant potassium channel."
    Lee S.-C., Lan W.-Z., Kim B.-G., Li L., Cheong Y.H., Pandey G.K., Lu G., Buchanan B.B., Luan S.
    Proc. Natl. Acad. Sci. U.S.A. 104:15959-15964(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION, INTERACTION WITH AIP1; CIPK6; CIPK16 AND CIPK23.
  16. "Calcineurin B-like protein CBL10 directly interacts with AKT1 and modulates K+ homeostasis in Arabidopsis."
    Ren X.L., Qi G.N., Feng H.Q., Zhao S., Zhao S.S., Wang Y., Wu W.H.
    Plant J. 74:258-266(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CBL10.

Entry informationi

Entry nameiAKT1_ARATH
AccessioniPrimary (citable) accession number: Q38998
Secondary accession number(s): Q0WTF6, Q38797, Q84MA7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: January 1, 1998
Last modified: October 29, 2014
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3