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Q38998

- AKT1_ARATH

UniProt

Q38998 - AKT1_ARATH

Protein

Potassium channel AKT1

Gene

AKT1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 2 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Highly selective inward-rectifying potassium channel that mediate potassium uptake by plant roots in response to low K+ conditions, by a calcium-, CBL-, and CIPK-dependent pathway. Positively regulated by phosphorylation by CIPK23. Negatively regulated by a kinase-independent regulatory mechanism involving a competing direct binding of CBL10. Involved in the stomatal regulation by monitoring the turgor pressure in guard cells. Assuming opened or closed conformations in response to the voltage difference across the membrane, the channel is activated by hyperpolarization. May interact with the cytoskeleton or with regulatory proteins.5 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi372 – 493122cNMPAdd
    BLAST

    GO - Molecular functioni

    1. identical protein binding Source: IntAct
    2. inward rectifier potassium channel activity Source: TAIR
    3. protein binding Source: IntAct

    GO - Biological processi

    1. potassium ion import Source: TAIR
    2. potassium ion transport Source: TAIR
    3. regulation of stomatal closure Source: TAIR
    4. response to salt stress Source: TAIR
    5. response to water deprivation Source: TAIR
    6. root hair elongation Source: TAIR

    Keywords - Molecular functioni

    Ion channel, Potassium channel, Voltage-gated channel

    Keywords - Biological processi

    Ion transport, Potassium transport, Transport

    Keywords - Ligandi

    Potassium

    Enzyme and pathway databases

    BioCyciARA:AT2G26550-MONOMER.
    MetaCyc:MONOMER-14552.

    Protein family/group databases

    TCDBi1.A.1.4.1. the voltage-gated ion channel (vic) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Potassium channel AKT1
    Gene namesi
    Name:AKT1
    Ordered Locus Names:At2g26650
    ORF Names:F18A8.2
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G26650.

    Subcellular locationi

    Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 857857Potassium channel AKT1PRO_0000054121Add
    BLAST

    Post-translational modificationi

    Phosphorylated by CIPK proteins CIPK6, CIPK16 and CIPK23. The activation by phosphorylation is induced by low K+ conditions and stimulates K+ uptake and relocation. Dephosphorylation by AIP1 repressed the transport activity.3 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ38998.
    PRIDEiQ38998.

    Expressioni

    Tissue specificityi

    Preferentially expressed in the peripheral cell layers of root mature including root cortex and root hairs. Detected also, at a lower level, in the mesophyll of the leaves and at restricted sites corresponding to hydathodes and guard cells.2 Publications

    Inductioni

    In roots, strongly reduced after 2,4-dichlorophenoxyacetic acid (2,4-D) treatment and weakly reduced after benzyladenine (BA) treatment. In shoots, strongly reduced after abscisic acid (ABA) treatment and induced after benzyladenine (BA) treatment.1 Publication

    Gene expression databases

    GenevestigatoriQ38998.

    Interactioni

    Subunit structurei

    The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming subunits. Possible heteromultimer with AKT2 or KAT3. Part of a K+-channel calcium-sensing kinase/phosphatase complex composed by a calcium sensor CBL (CBL1, CBL2, CBL3 or CBL9), a kinase CIPK (CIPK6, CIPK16 or CIPK23), a phosphatase PP2C (AIP1) and a K+-channel (AKT1). Interacts directly with AIP1, CBL10, CIPK6, CIPK16 and CIPK23.6 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself7EBI-974289,EBI-974289
    AKT2Q388983EBI-974289,EBI-1552774
    CIPK23Q93VD34EBI-974289,EBI-974277

    Protein-protein interaction databases

    BioGridi2558. 12 interactions.
    DIPiDIP-36762N.
    IntActiQ38998. 7 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ38998.
    SMRiQ38998. Positions 190-299, 485-840.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 6161CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini83 – 908ExtracellularSequence Analysis
    Topological domaini112 – 13423CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini156 – 1583ExtracellularSequence Analysis
    Topological domaini180 – 19314CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini215 – 24127ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini262 – 2654ExtracellularSequence Analysis
    Topological domaini287 – 857571CytoplasmicSequence AnalysisAdd
    BLAST

    Intramembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Intramembranei242 – 26120Pore-forming; Name=Segment H5Sequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei62 – 8221Helical; Name=Segment S1Sequence AnalysisAdd
    BLAST
    Transmembranei91 – 11121Helical; Name=Segment S2Sequence AnalysisAdd
    BLAST
    Transmembranei135 – 15521Helical; Name=Segment S3Sequence AnalysisAdd
    BLAST
    Transmembranei159 – 17921Helical; Voltage-sensor; Name=Segment S4Sequence AnalysisAdd
    BLAST
    Transmembranei194 – 21421Helical; Name=Segment S5Sequence AnalysisAdd
    BLAST
    Transmembranei266 – 28621Helical; Name=Segment S6Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati515 – 54632ANK 1Add
    BLAST
    Repeati550 – 57930ANK 2Add
    BLAST
    Repeati583 – 61230ANK 3Add
    BLAST
    Repeati614 – 64330ANK 4Add
    BLAST
    Repeati647 – 67630ANK 5Add
    BLAST
    Repeati680 – 70930ANK 6Add
    BLAST
    Domaini790 – 85768KHAPROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids. The pore-forming region H5 is enclosed by the transmembrane segments S5 and S6 in the Shaker-type (1P/6TM) and contains the GYGD signature motif which seems to be involved in potassium selectivity.
    The KHA domain (rich in hydrophobic and acidic residues) present in the C-terminal part is likely to be important for tetramerization.

    Sequence similaritiesi

    Contains 6 ANK repeats.PROSITE-ProRule annotation
    Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation
    Contains 1 KHA domain.PROSITE-ProRule annotation

    Keywords - Domaini

    ANK repeat, Repeat, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0666.
    HOGENOMiHOG000238230.
    InParanoidiQ38998.
    OMAiAEQQGHE.
    PhylomeDBiQ38998.

    Family and domain databases

    Gene3Di1.25.40.20. 1 hit.
    2.60.120.10. 1 hit.
    InterProiIPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    IPR018490. cNMP-bd-like.
    IPR000595. cNMP-bd_dom.
    IPR005821. Ion_trans_dom.
    IPR021789. K_channel_plant.
    IPR003938. K_chnl_volt-dep_EAG/ELK/ERG.
    IPR014710. RmlC-like_jellyroll.
    [Graphical view]
    PfamiPF00023. Ank. 3 hits.
    PF00027. cNMP_binding. 1 hit.
    PF11834. DUF3354. 1 hit.
    PF00520. Ion_trans. 1 hit.
    [Graphical view]
    PRINTSiPR01415. ANKYRIN.
    PR01463. EAGCHANLFMLY.
    SMARTiSM00248. ANK. 5 hits.
    SM00100. cNMP. 1 hit.
    [Graphical view]
    SUPFAMiSSF48403. SSF48403. 1 hit.
    SSF51206. SSF51206. 1 hit.
    PROSITEiPS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 3 hits.
    PS50042. CNMP_BINDING_3. 1 hit.
    PS51490. KHA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q38998-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRGGALLCGQ VQDEIEQLSR ESSHFSLSTG ILPSLGARSN RRVKLRRFVV    50
    SPYDHKYRIW EAFLVVLVVY TAWVSPFEFG FLRKPRPPLS ITDNIVNAFF 100
    AIDIIMTFFV GYLDKSTYLI VDDRKQIAFK YLRSWFLLDL VSTIPSEAAM 150
    RISSQSYGLF NMLRLWRLRR VGALFARLEK DRNFNYFWVR CAKLVCVTLF 200
    AVHCAACFYY LIAARNSNPA KTWIGANVAN FLEESLWMRY VTSMYWSITT 250
    LTTVGYGDLH PVNTKEMIFD IFYMLFNLGL TAYLIGNMTN LVVHGTSRTR 300
    NFRDTIQAAS NFAHRNHLPP RLQDQMLAHL CLKYRTDSEG LQQQETLDAL 350
    PKAIRSSISH FLFYSLMDKV YLFRGVSNDL LFQLVSEMKA EYFPPKEDVI 400
    LQNEAPTDFY ILVNGTADLV DVDTGTESIV REVKAGDIIG EIGVLCYRPQ 450
    LFTVRTKRLC QLLRMNRTTF LNIIQANVGD GTIIMNNLLQ HLKEMNDPVM 500
    TNVLLEIENM LARGKMDLPL NLCFAAIRED DLLLHQLLKR GLDPNESDNN 550
    GRTPLHIAAS KGTLNCVLLL LEYHADPNCR DAEGSVPLWE AMVEGHEKVV 600
    KVLLEHGSTI DAGDVGHFAC TAAEQGNLKL LKEIVLHGGD VTRPRATGTS 650
    ALHTAVCEEN IEMVKYLLEQ GADVNKQDMH GWTPRDLAEQ QGHEDIKALF 700
    REKLHERRVH IETSSSVPIL KTGIRFLGRF TSEPNIRPAS REVSFRIRET 750
    RARRKTNNFD NSLFGILANQ SVPKNGLATV DEGRTGNPVR VTISCAEKDD 800
    IAGKLVLLPG SFKELLELGS NKFGIVATKV MNKDNNAEID DVDVIRDGDH 850
    LIFATDS 857
    Length:857
    Mass (Da):96,990
    Last modified:January 1, 1998 - v2
    Checksum:i28E1622BA3505F4C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X62907 mRNA. Translation: CAA44693.1.
    U06745 Genomic DNA. Translation: AAA96810.1.
    AC003105 Genomic DNA. Translation: AAB95299.1.
    CP002685 Genomic DNA. Translation: AEC07870.1.
    AK227601 mRNA. Translation: BAE99592.1.
    BT006442 mRNA. Translation: AAP21250.1.
    PIRiS23606.
    S62694.
    RefSeqiNP_180233.1. NM_128222.5.
    UniGeneiAt.48521.
    At.68413.

    Genome annotation databases

    EnsemblPlantsiAT2G26650.1; AT2G26650.1; AT2G26650.
    GeneIDi817206.
    KEGGiath:AT2G26650.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X62907 mRNA. Translation: CAA44693.1 .
    U06745 Genomic DNA. Translation: AAA96810.1 .
    AC003105 Genomic DNA. Translation: AAB95299.1 .
    CP002685 Genomic DNA. Translation: AEC07870.1 .
    AK227601 mRNA. Translation: BAE99592.1 .
    BT006442 mRNA. Translation: AAP21250.1 .
    PIRi S23606.
    S62694.
    RefSeqi NP_180233.1. NM_128222.5.
    UniGenei At.48521.
    At.68413.

    3D structure databases

    ProteinModelPortali Q38998.
    SMRi Q38998. Positions 190-299, 485-840.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 2558. 12 interactions.
    DIPi DIP-36762N.
    IntActi Q38998. 7 interactions.

    Protein family/group databases

    TCDBi 1.A.1.4.1. the voltage-gated ion channel (vic) superfamily.

    Proteomic databases

    PaxDbi Q38998.
    PRIDEi Q38998.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G26650.1 ; AT2G26650.1 ; AT2G26650 .
    GeneIDi 817206.
    KEGGi ath:AT2G26650.

    Organism-specific databases

    GeneFarmi 2792. 266.
    TAIRi AT2G26650.

    Phylogenomic databases

    eggNOGi COG0666.
    HOGENOMi HOG000238230.
    InParanoidi Q38998.
    OMAi AEQQGHE.
    PhylomeDBi Q38998.

    Enzyme and pathway databases

    BioCyci ARA:AT2G26550-MONOMER.
    MetaCyc:MONOMER-14552.

    Gene expression databases

    Genevestigatori Q38998.

    Family and domain databases

    Gene3Di 1.25.40.20. 1 hit.
    2.60.120.10. 1 hit.
    InterProi IPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    IPR018490. cNMP-bd-like.
    IPR000595. cNMP-bd_dom.
    IPR005821. Ion_trans_dom.
    IPR021789. K_channel_plant.
    IPR003938. K_chnl_volt-dep_EAG/ELK/ERG.
    IPR014710. RmlC-like_jellyroll.
    [Graphical view ]
    Pfami PF00023. Ank. 3 hits.
    PF00027. cNMP_binding. 1 hit.
    PF11834. DUF3354. 1 hit.
    PF00520. Ion_trans. 1 hit.
    [Graphical view ]
    PRINTSi PR01415. ANKYRIN.
    PR01463. EAGCHANLFMLY.
    SMARTi SM00248. ANK. 5 hits.
    SM00100. cNMP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48403. SSF48403. 1 hit.
    SSF51206. SSF51206. 1 hit.
    PROSITEi PS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 3 hits.
    PS50042. CNMP_BINDING_3. 1 hit.
    PS51490. KHA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression in yeast of a plant potassium ion transport system."
      Sentenac H., Bonneaud N., Minet M., Lacroute F., Salmon J.-M., Gaymard F., Grignon C.
      Science 256:663-665(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Landsberg erecta.
    2. Sentenac H.
      Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION.
    3. "Organization and expression of the gene coding for the potassium transport system AKT1 of Arabidopsis thaliana."
      Basset M., Conejero G., Lepetit M., Fourcroy P., Sentenac H.
      Plant Mol. Biol. 29:947-958(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
      Strain: cv. Landsberg erecta.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    5. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    6. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 79-857.
      Strain: cv. Columbia.
    7. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 106-857.
      Strain: cv. Columbia.
    8. "Tissue-specific expression of Arabidopsis AKT1 gene is consistent with a role in K+ nutrition."
      Lagarde D., Basset M., Lepetit M., Conejero G., Gaymard F., Astruc S., Grignon C.
      Plant J. 9:195-203(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    9. "Tetramerization of the AKT1 plant potassium channel involves its C-terminal cytoplasmic domain."
      Daram P., Urbach S., Gaymard F., Sentenac H., Cherel I.
      EMBO J. 16:3455-3463(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT.
    10. "A role for the AKT1 potassium channel in plant nutrition."
      Hirsch R.E., Lewis B.D., Spalding E.P., Sussman M.R.
      Science 280:918-921(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    11. Cited for: GENE FAMILY, NOMENCLATURE.
    12. "Regulated expression of Arabidopsis shaker K(+) channel genes involved in K(+) uptake and distribution in the plant."
      Pilot G., Gaymard F., Mouline K., Cherel I., Sentenac H.
      Plant Mol. Biol. 51:773-787(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH AKT2 AND KAT3, INDUCTION.
    13. "A protein kinase, interacting with two calcineurin B-like proteins, regulates K+ transporter AKT1 in Arabidopsis."
      Xu J., Li H.-D., Chen L.-Q., Wang Y., Liu L.-L., He L., Wu W.-H.
      Cell 125:1347-1360(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH CIPK23, PHOSPHORYLATION, SUBCELLULAR LOCATION.
    14. "A Ca(2)+ signaling pathway regulates a K(+) channel for low-K response in Arabidopsis."
      Li L., Kim B.-G., Cheong Y.H., Pandey G.K., Luan S.
      Proc. Natl. Acad. Sci. U.S.A. 103:12625-12630(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH CIPK23, PHOSPHORYLATION.
    15. "A protein phosphorylation/dephosphorylation network regulates a plant potassium channel."
      Lee S.-C., Lan W.-Z., Kim B.-G., Li L., Cheong Y.H., Pandey G.K., Lu G., Buchanan B.B., Luan S.
      Proc. Natl. Acad. Sci. U.S.A. 104:15959-15964(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, PHOSPHORYLATION, INTERACTION WITH AIP1; CIPK6; CIPK16 AND CIPK23.
    16. "Calcineurin B-like protein CBL10 directly interacts with AKT1 and modulates K+ homeostasis in Arabidopsis."
      Ren X.L., Qi G.N., Feng H.Q., Zhao S., Zhao S.S., Wang Y., Wu W.H.
      Plant J. 74:258-266(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CBL10.

    Entry informationi

    Entry nameiAKT1_ARATH
    AccessioniPrimary (citable) accession number: Q38998
    Secondary accession number(s): Q0WTF6, Q38797, Q84MA7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 2004
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 121 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3