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Protein

SNF1-related protein kinase catalytic subunit alpha KIN10

Gene

KIN10

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic subunit of the probable trimeric SNF1-related protein kinase (SnRK) complex, which may play a role in a signal transduction cascade regulating gene expression and carbohydrate metabolism in higher plants. The SnRK complex may also be involved in the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase and in assimilation of nitrogen by phosphorylating nitrate reductase. In vitro, KIN10 exhibits kinase activity on sucrose phosphate synthase and the kinase activity is inhibited by PRL1. May be a subunit of a SCF ubiquitin ligase complex and thus be involved in proteasomal ubiquitination. Phosphorylates GRIK1/SNAK2 and GRIK2/SNAK1 in vitro.3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by phosphorylation at Thr-198.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei71ATPPROSITE-ProRule annotation1
Active sitei165Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi48 – 56ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein serine/threonine kinase activity Source: TAIR

GO - Biological processi

  • abscisic acid-activated signaling pathway Source: TAIR
  • carbohydrate metabolic process Source: UniProtKB-KW
  • detection of nutrient Source: TAIR
  • developmental process involved in reproduction Source: TAIR
  • fatty acid biosynthetic process Source: UniProtKB-KW
  • intracellular signal transduction Source: GO_Central
  • leaf senescence Source: TAIR
  • nitrate assimilation Source: UniProtKB-KW
  • primary root development Source: TAIR
  • sugar mediated signaling pathway Source: TAIR
  • vegetative phase change Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Nitrate assimilation, Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 399.
ReactomeiR-ATH-163680. AMPK inhibits chREBP transcriptional activation activity.
R-ATH-200425. Import of palmitoyl-CoA into the mitochondrial matrix.
R-ATH-380972. Energy dependent regulation of mTOR by LKB1-AMPK.

Names & Taxonomyi

Protein namesi
Recommended name:
SNF1-related protein kinase catalytic subunit alpha KIN10 (EC:2.7.11.1)
Short name:
AKIN10
Alternative name(s):
AKIN alpha-2
Short name:
AKINalpha2
Gene namesi
Name:KIN10
Synonyms:AK21, SKIN10, SNR2, SNRK1.1
Ordered Locus Names:At3g01090
ORF Names:T4P13.22
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G01090.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi198T → A: Abolishes phosphorylation by GRIK1 or GRIK2 leading to inactivation of the protein. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000861281 – 535SNF1-related protein kinase catalytic subunit alpha KIN10Add BLAST535

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki43Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei187PhosphoserineBy similarity1
Modified residuei198Phosphothreonine; by GRIK1 or GRIK21 Publication1

Post-translational modificationi

Autophosphorylated. Phosphorylated at Thr-198 by GRIK1/SNAK2 and GRIK2/SNAK1.2 Publications

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ38997.
PRIDEiQ38997.

PTM databases

iPTMnetiQ38997.

Expressioni

Inductioni

Induced by sucrose.1 Publication

Gene expression databases

ExpressionAtlasiQ38997. baseline and differential.
GenevisibleiQ38997. AT.

Interactioni

Subunit structurei

Subunit of a probable heterotrimeric complex consisting of an alpha catalytic (KIN10 or KIN11) subunit, and a beta (KINB) and a gamma (KING or SNF4) non-catalytic regulatory subunits. Interacts with KINB2, KINB3, SNF4 and probably with KINB1 and KING1. Interacts with SKP1/ASK1, PAD1, the N-terminus of PRL1 and the WD40 domain of 5PTase13. Potential subunit of a SCF ubiquitin ligase complex consisting of a SNF1-related protein kinase, SKP1 and CUL1. The association of the SCF complex with the proteasome may be mediated by PAD1 and seems to be inhibited by the interaction with PRL1. Interacts with DSP4.6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
GRIK1Q93V582EBI-2107143,EBI-6399184
GRIK2Q5HZ382EBI-2107143,EBI-6399237
KINB2Q9SCY53EBI-2107143,EBI-2042436
PRL1Q423843EBI-2107143,EBI-1382964
SNF4Q944A62EBI-2107143,EBI-2360649

Protein-protein interaction databases

BioGridi6592. 36 interactors.
IntActiQ38997. 9 interactors.
STRINGi3702.AT3G01090.2.

Structurei

3D structure databases

ProteinModelPortaliQ38997.
SMRiQ38997.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini42 – 294Protein kinasePROSITE-ProRule annotationAdd BLAST253
Domaini315 – 355UBAPROSITE-ProRule annotationAdd BLAST41
Domaini486 – 534KA1PROSITE-ProRule annotationAdd BLAST49

Sequence similaritiesi

Contains 1 KA1 (kinase-associated) domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 UBA domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0583. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000233016.
InParanoidiQ38997.
KOiK07198.
OMAiEIREHVW.
OrthoDBiEOG0936066G.
PhylomeDBiQ38997.

Family and domain databases

Gene3Di3.30.310.80. 1 hit.
InterProiIPR028375. KA1/Ssp2_C.
IPR001772. KA1_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR015940. UBA.
IPR009060. UBA-like.
[Graphical view]
PfamiPF02149. KA1. 1 hit.
PF00069. Pkinase. 1 hit.
PF00627. UBA. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
SM00165. UBA. 1 hit.
[Graphical view]
SUPFAMiSSF103243. SSF103243. 1 hit.
SSF46934. SSF46934. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50032. KA1. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50030. UBA. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q38997-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFKRVDEFNL VSSTIDHRIF KSRMDGSGTG SRSGVESILP NYKLGRTLGI
60 70 80 90 100
GSFGRVKIAE HALTGHKVAI KILNRRKIKN MEMEEKVRRE IKILRLFMHP
110 120 130 140 150
HIIRLYEVIE TPTDIYLVME YVNSGELFDY IVEKGRLQED EARNFFQQII
160 170 180 190 200
SGVEYCHRNM VVHRDLKPEN LLLDSKCNVK IADFGLSNIM RDGHFLKTSC
210 220 230 240 250
GSPNYAAPEV ISGKLYAGPE VDVWSCGVIL YALLCGTLPF DDENIPNLFK
260 270 280 290 300
KIKGGIYTLP SHLSPGARDL IPRMLVVDPM KRVTIPEIRQ HPWFQAHLPR
310 320 330 340 350
YLAVPPPDTV QQAKKIDEEI LQEVINMGFD RNHLIESLRN RTQNDGTVTY
360 370 380 390 400
YLILDNRFRA SSGYLGAEFQ ETMEGTPRMH PAESVASPVS HRLPGLMEYQ
410 420 430 440 450
GVGLRSQYPV ERKWALGLQS RAHPREIMTE VLKALQDLNV CWKKIGHYNM
460 470 480 490 500
KCRWVPNSSA DGMLSNSMHD NNYFGDESSI IENEAAVKSP NVVKFEIQLY
510 520 530
KTRDDKYLLD LQRVQGPQFL FLDLCAAFLA QLRVL
Note: No experimental confirmation available.
Length:535
Mass (Da):61,182
Last modified:November 21, 2003 - v2
Checksum:iFFFC383223FD8317
GO
Isoform 2 (identifier: Q38997-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-23: Missing.

Show »
Length:512
Mass (Da):58,373
Checksum:i5A18655A0AA506DF
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0090011 – 23Missing in isoform 2. 2 PublicationsAdd BLAST23

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93023 Genomic DNA. Translation: AAA32736.1.
X94757 mRNA. Translation: CAA64384.1.
DQ778957 mRNA. Translation: ABH11527.1.
AC008261 Genomic DNA. Translation: AAF26165.1.
CP002686 Genomic DNA. Translation: AEE73607.1.
CP002686 Genomic DNA. Translation: AEE73608.1.
CP002686 Genomic DNA. Translation: AEE73609.1.
AY093170 mRNA. Translation: AAM13169.1.
BT010386 mRNA. Translation: AAQ56829.1.
X79707 Genomic DNA. Translation: CAA56146.1.
X86966 Genomic DNA. Translation: CAA60529.1.
PIRiJC1446.
RefSeqiNP_001118546.1. NM_001125074.2. [Q38997-2]
NP_566130.1. NM_110974.5. [Q38997-2]
NP_850488.1. NM_180157.1. [Q38997-1]
UniGeneiAt.22965.

Genome annotation databases

EnsemblPlantsiAT3G01090.2; AT3G01090.2; AT3G01090. [Q38997-1]
GeneIDi821259.
GrameneiAT3G01090.2; AT3G01090.2; AT3G01090.
KEGGiath:AT3G01090.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

PlantP kinase Classification PPC

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93023 Genomic DNA. Translation: AAA32736.1.
X94757 mRNA. Translation: CAA64384.1.
DQ778957 mRNA. Translation: ABH11527.1.
AC008261 Genomic DNA. Translation: AAF26165.1.
CP002686 Genomic DNA. Translation: AEE73607.1.
CP002686 Genomic DNA. Translation: AEE73608.1.
CP002686 Genomic DNA. Translation: AEE73609.1.
AY093170 mRNA. Translation: AAM13169.1.
BT010386 mRNA. Translation: AAQ56829.1.
X79707 Genomic DNA. Translation: CAA56146.1.
X86966 Genomic DNA. Translation: CAA60529.1.
PIRiJC1446.
RefSeqiNP_001118546.1. NM_001125074.2. [Q38997-2]
NP_566130.1. NM_110974.5. [Q38997-2]
NP_850488.1. NM_180157.1. [Q38997-1]
UniGeneiAt.22965.

3D structure databases

ProteinModelPortaliQ38997.
SMRiQ38997.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi6592. 36 interactors.
IntActiQ38997. 9 interactors.
STRINGi3702.AT3G01090.2.

PTM databases

iPTMnetiQ38997.

Proteomic databases

PaxDbiQ38997.
PRIDEiQ38997.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G01090.2; AT3G01090.2; AT3G01090. [Q38997-1]
GeneIDi821259.
GrameneiAT3G01090.2; AT3G01090.2; AT3G01090.
KEGGiath:AT3G01090.

Organism-specific databases

TAIRiAT3G01090.

Phylogenomic databases

eggNOGiKOG0583. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000233016.
InParanoidiQ38997.
KOiK07198.
OMAiEIREHVW.
OrthoDBiEOG0936066G.
PhylomeDBiQ38997.

Enzyme and pathway databases

BRENDAi2.7.11.1. 399.
ReactomeiR-ATH-163680. AMPK inhibits chREBP transcriptional activation activity.
R-ATH-200425. Import of palmitoyl-CoA into the mitochondrial matrix.
R-ATH-380972. Energy dependent regulation of mTOR by LKB1-AMPK.

Miscellaneous databases

PROiQ38997.

Gene expression databases

ExpressionAtlasiQ38997. baseline and differential.
GenevisibleiQ38997. AT.

Family and domain databases

Gene3Di3.30.310.80. 1 hit.
InterProiIPR028375. KA1/Ssp2_C.
IPR001772. KA1_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR015940. UBA.
IPR009060. UBA-like.
[Graphical view]
PfamiPF02149. KA1. 1 hit.
PF00069. Pkinase. 1 hit.
PF00627. UBA. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
SM00165. UBA. 1 hit.
[Graphical view]
SUPFAMiSSF103243. SSF103243. 1 hit.
SSF46934. SSF46934. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50032. KA1. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50030. UBA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKIN10_ARATH
AccessioniPrimary (citable) accession number: Q38997
Secondary accession number(s): A6XGR0
, O04728, Q38987, Q39076, Q8RWD2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 21, 2003
Last modified: November 30, 2016
This is version 162 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.