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Q38946

- DHE2_ARATH

UniProt

Q38946 - DHE2_ARATH

Protein

Glutamate dehydrogenase 2

Gene

GDH2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    L-glutamate + H2O + NAD(P)+ = 2-oxoglutarate + NH3 + NAD(P)H.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei102 – 1021PROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: TAIR
    2. cobalt ion binding Source: TAIR
    3. copper ion binding Source: TAIR
    4. glutamate dehydrogenase (NAD+) activity Source: TAIR
    5. glutamate dehydrogenase [NAD(P)+] activity Source: TAIR
    6. zinc ion binding Source: TAIR

    GO - Biological processi

    1. cellular amino acid metabolic process Source: InterPro
    2. response to cadmium ion Source: TAIR
    3. response to salt stress Source: TAIR

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciARA:AT5G07440-MONOMER.
    ARA:GQT-326-MONOMER.
    ARA:GQT-327-MONOMER.
    MetaCyc:AT5G07440-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate dehydrogenase 2 (EC:1.4.1.3)
    Short name:
    GDH 2
    Gene namesi
    Name:GDH2
    Ordered Locus Names:At5g07440
    ORF Names:T2I1_150
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G07440.

    Subcellular locationi

    Mitochondrion 2 Publications

    GO - Cellular componenti

    1. mitochondrion Source: TAIR
    2. vacuolar membrane Source: TAIR

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 411411Glutamate dehydrogenase 2PRO_0000182746Add
    BLAST

    Proteomic databases

    PaxDbiQ38946.
    PRIDEiQ38946.

    Expressioni

    Gene expression databases

    GenevestigatoriQ38946.

    Structurei

    3D structure databases

    ProteinModelPortaliQ38946.
    SMRiQ38946. Positions 11-408.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0334.
    HOGENOMiHOG000243801.
    InParanoidiQ38946.
    KOiK00261.
    OMAiFNFDRAK.
    PhylomeDBiQ38946.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR006095. Glu/Leu/Phe/Val_DH.
    IPR006096. Glu/Leu/Phe/Val_DH_C.
    IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
    IPR014362. Glu_DH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF00208. ELFV_dehydrog. 1 hit.
    PF02812. ELFV_dehydrog_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000185. Glu_DH. 1 hit.
    PRINTSiPR00082. GLFDHDRGNASE.
    SMARTiSM00839. ELFV_dehydrog. 1 hit.
    [Graphical view]
    PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: Q38946-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MNALAATNRN FRHASRILGL DSKIERSLMI PFREIKVECT IPKDDGTLVS    50
    YIGFRVQHDN ARGPMKGGIR YHPEVDPDEV NALAQLMTWK TAVADIPYGG 100
    AKGGIGCSPR DLSLSELERL TRVFTQKIHD LIGIHTDVPA PDMGTNAQTM 150
    AWILDEYSKF HGHSPAVVTG KPIDLGGSLG REAATGRGVV FATEALLAEY 200
    GKSIQGLTFV IQGFGNVGTW AAKLIHEKGG KVVAVSDITG AIRNPEGIDI 250
    NALIKHKDAT GSLNDFNGGD AMNSDELLIH ECDVLIPCAL GGVLNKENAG 300
    DVKAKFIVEA ANHPTDPDAD EILSKKGVII LPDIYANAGG VTVSYFEWVQ 350
    NIQGFMWEEE KVNLELQKYM TRAFHNIKTM CHTHSCNLRM GAFTLGVNRV 400
    ARATQLRGWE A 411
    Length:411
    Mass (Da):44,699
    Last modified:November 1, 1996 - v1
    Checksum:i91267768215A15CF
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti232 – 2321V → I AA sequence (PubMed:11743114)Curated
    Sequence conflicti242 – 2432IR → LK AA sequence (PubMed:11743114)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U56635 mRNA. Translation: AAB01222.1.
    AL163912 Genomic DNA. Translation: CAB87933.1.
    CP002688 Genomic DNA. Translation: AED91157.1.
    CP002688 Genomic DNA. Translation: AED91158.1.
    PIRiT49883.
    RefSeqiNP_001119183.1. NM_001125711.1. [Q38946-1]
    NP_196361.1. NM_120826.2. [Q38946-1]
    UniGeneiAt.21712.

    Genome annotation databases

    EnsemblPlantsiAT5G07440.1; AT5G07440.1; AT5G07440. [Q38946-1]
    AT5G07440.2; AT5G07440.2; AT5G07440. [Q38946-1]
    GeneIDi830635.
    KEGGiath:AT5G07440.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U56635 mRNA. Translation: AAB01222.1 .
    AL163912 Genomic DNA. Translation: CAB87933.1 .
    CP002688 Genomic DNA. Translation: AED91157.1 .
    CP002688 Genomic DNA. Translation: AED91158.1 .
    PIRi T49883.
    RefSeqi NP_001119183.1. NM_001125711.1. [Q38946-1 ]
    NP_196361.1. NM_120826.2. [Q38946-1 ]
    UniGenei At.21712.

    3D structure databases

    ProteinModelPortali Q38946.
    SMRi Q38946. Positions 11-408.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi Q38946.
    PRIDEi Q38946.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G07440.1 ; AT5G07440.1 ; AT5G07440 . [Q38946-1 ]
    AT5G07440.2 ; AT5G07440.2 ; AT5G07440 . [Q38946-1 ]
    GeneIDi 830635.
    KEGGi ath:AT5G07440.

    Organism-specific databases

    GeneFarmi 2247. 178.
    TAIRi AT5G07440.

    Phylogenomic databases

    eggNOGi COG0334.
    HOGENOMi HOG000243801.
    InParanoidi Q38946.
    KOi K00261.
    OMAi FNFDRAK.
    PhylomeDBi Q38946.

    Enzyme and pathway databases

    BioCyci ARA:AT5G07440-MONOMER.
    ARA:GQT-326-MONOMER.
    ARA:GQT-327-MONOMER.
    MetaCyc:AT5G07440-MONOMER.

    Miscellaneous databases

    PROi Q38946.

    Gene expression databases

    Genevestigatori Q38946.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR006095. Glu/Leu/Phe/Val_DH.
    IPR006096. Glu/Leu/Phe/Val_DH_C.
    IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
    IPR014362. Glu_DH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF00208. ELFV_dehydrog. 1 hit.
    PF02812. ELFV_dehydrog_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000185. Glu_DH. 1 hit.
    PRINTSi PR00082. GLFDHDRGNASE.
    SMARTi SM00839. ELFV_dehydrog. 1 hit.
    [Graphical view ]
    PROSITEi PS00074. GLFV_DEHYDROGENASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Turano F.J., Thakkar S.S., Weisemann J.M.
      Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
      Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
      , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
      Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Proteomic approach to identify novel mitochondrial proteins in Arabidopsis."
      Kruft V., Eubel H., Jaensch L., Werhahn W., Braun H.-P.
      Plant Physiol. 127:1694-1710(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 232-243 AND 391-399, SUBCELLULAR LOCATION.
      Tissue: Leaf and Stem.
    5. "Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
      Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
      Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Strain: cv. Landsberg erecta.

    Entry informationi

    Entry nameiDHE2_ARATH
    AccessioniPrimary (citable) accession number: Q38946
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3