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Q38946 (DHE2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate dehydrogenase 2

Short name=GDH 2
EC=1.4.1.3
Gene names
Name:GDH2
Ordered Locus Names:At5g07440
ORF Names:T2I1_150
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length411 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

L-glutamate + H2O + NAD(P)+ = 2-oxoglutarate + NH3 + NAD(P)H.

Subcellular location

Mitochondrion Ref.4 Ref.5.

Sequence similarities

Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q38946-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 411411Glutamate dehydrogenase 2
PRO_0000182746

Sites

Active site1021 By similarity

Experimental info

Sequence conflict2321V → I AA sequence Ref.4
Sequence conflict242 – 2432IR → LK AA sequence Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 91267768215A15CF

FASTA41144,699
        10         20         30         40         50         60 
MNALAATNRN FRHASRILGL DSKIERSLMI PFREIKVECT IPKDDGTLVS YIGFRVQHDN 

        70         80         90        100        110        120 
ARGPMKGGIR YHPEVDPDEV NALAQLMTWK TAVADIPYGG AKGGIGCSPR DLSLSELERL 

       130        140        150        160        170        180 
TRVFTQKIHD LIGIHTDVPA PDMGTNAQTM AWILDEYSKF HGHSPAVVTG KPIDLGGSLG 

       190        200        210        220        230        240 
REAATGRGVV FATEALLAEY GKSIQGLTFV IQGFGNVGTW AAKLIHEKGG KVVAVSDITG 

       250        260        270        280        290        300 
AIRNPEGIDI NALIKHKDAT GSLNDFNGGD AMNSDELLIH ECDVLIPCAL GGVLNKENAG 

       310        320        330        340        350        360 
DVKAKFIVEA ANHPTDPDAD EILSKKGVII LPDIYANAGG VTVSYFEWVQ NIQGFMWEEE 

       370        380        390        400        410 
KVNLELQKYM TRAFHNIKTM CHTHSCNLRM GAFTLGVNRV ARATQLRGWE A 

« Hide

References

« Hide 'large scale' references
[1]Turano F.J., Thakkar S.S., Weisemann J.M.
Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. expand/collapse author list , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Proteomic approach to identify novel mitochondrial proteins in Arabidopsis."
Kruft V., Eubel H., Jaensch L., Werhahn W., Braun H.-P.
Plant Physiol. 127:1694-1710(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 232-243 AND 391-399, SUBCELLULAR LOCATION.
Tissue: Leaf and Stem.
[5]"Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Strain: cv. Landsberg erecta.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U56635 mRNA. Translation: AAB01222.1.
AL163912 Genomic DNA. Translation: CAB87933.1.
CP002688 Genomic DNA. Translation: AED91157.1.
CP002688 Genomic DNA. Translation: AED91158.1.
PIRT49883.
RefSeqNP_001119183.1. NM_001125711.1.
NP_196361.1. NM_120826.2.
UniGeneAt.21712.

3D structure databases

ProteinModelPortalQ38946.
SMRQ38946. Positions 11-408.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbQ38946.
PRIDEQ38946.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G07440.1; AT5G07440.1; AT5G07440. [Q38946-1]
AT5G07440.2; AT5G07440.2; AT5G07440. [Q38946-1]
GeneID830635.
KEGGath:AT5G07440.

Organism-specific databases

GeneFarm2247. 178.
TAIRAT5G07440.

Phylogenomic databases

eggNOGCOG0334.
HOGENOMHOG000243801.
InParanoidQ38946.
KOK00261.
OMAHIKAMCK.
PhylomeDBQ38946.
ProtClustDBPLN02477.

Enzyme and pathway databases

BioCycARA:AT5G07440-MONOMER.
ARA:GQT-326-MONOMER.
ARA:GQT-327-MONOMER.
MetaCyc:AT5G07440-MONOMER.

Gene expression databases

GenevestigatorQ38946.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR006095. Glu/Leu/Phe/Val_DH.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFPIRSF000185. Glu_DH. 1 hit.
PRINTSPR00082. GLFDHDRGNASE.
SMARTSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
PROSITEPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ38946.

Entry information

Entry nameDHE2_ARATH
AccessionPrimary (citable) accession number: Q38946
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names