Reviewed, UniProtKB/Swiss-Prot Q38945 (DPNPH_ARATH)

Names and origin

Protein names

Recommended name:
    PAP-specific phosphatase HAL2-like
Alternative name(s):
    3'(2'),5'-bisphosphate nucleotidase
    EC=3.1.3.7
    3'(2'),5-bisphosphonucleoside 3'(2')-phosphohydrolase
    DPNPase
    Halotolerance protein

Gene names

Name:	AHL
Ordered Locus Names:	At5g54390
ORF Names:	F24B18.1

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	373 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Converts adenosine 3'-phosphate 5'-phosphosulfate (PAPS) to adenosine 5'-phosphosulfate (APS) and 3'(2')-phosphoadenosine 5'- phosphate (PAP) to AMP. May regulate the flux of sulfur in the sulfur-activation pathway by converting PAPS to APS By similarity. Prevents both the toxicity of PAP on RNA processing enzymes as well as the product inhibition by PAP of sulfate conjugation.
Catalytic activity	Adenosine 3',5'-bisphosphate + H2O = adenosine 5'-phosphate + phosphate.
Cofactor	Magnesium By similarity.
Enzyme regulation	Inhibited by Li+ (IC50=10 millimolar), Na+ (IC50=50 millimolar) and Ca2+ (IC50=0.06 millimolar).
Tissue specificity	Expressed in roots, leaves, stems, flowers and siliques.
Miscellaneous	Substrate preference is 3'-phosphoadenosine 5'-phosphate (PAP) > adenosine 3'-phosphate 5'-phosphosulfate (PAPS). No activity observed against 3' or 5'-AMP, inositol mono and diphosphates and fructose-1,6-bisphosphate.
Sequence similarities	Belongs to the inositol monophosphatase family.

Ontologies

Keywords
Ligand	Calcium Lithium Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	sulfur metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	3'(2'),5'-bisphosphate nucleotidase activity Ref.3 Inferred from direct assay. Source: TAIR calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW inositol or phosphatidylinositol phosphatase activity Inferred from electronic annotation. Source: InterPro lithium ion binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 373

373

PAP-specific phosphatase HAL2-like

PRO_0000142534

Sites

Metal binding

77

1

Magnesium 1 By similarity

Metal binding

144

1

Magnesium 1 By similarity

Metal binding

144

1

Magnesium 2 By similarity

Metal binding

146

1

Magnesium 1; via carbonyl oxygen By similarity

Metal binding

147

1

Magnesium 2 By similarity

Metal binding

310

1

Magnesium 2 By similarity

Experimental info

Sequence conflict

107 – 108

2

QN → SK in AAB94051. Ref.2

Secondary structure

1

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373

Helix Strand Turn

Details...

Helix

7 – 32

26

Beta strand

39 – 41

3

Beta strand

47 – 49

3

Helix

50 – 63

14

Helix

64 – 66

3

Beta strand

76 – 79

4

Beta strand

82 – 86

5

Beta strand

99 – 102

4

Beta strand

110 – 113

4

Beta strand

132 – 137

6

Beta strand

141 – 146

6

Turn

147 – 149

3

Helix

150 – 154

5

Beta strand

161 – 166

6

Beta strand

169 – 174

6

Beta strand

179 – 181

3

Beta strand

212 – 216

5

Beta strand

218 – 220

3

Beta strand

231 – 235

5

Beta strand

242 – 244

3

Beta strand

255 – 258

4

Beta strand

264 – 266

3

Beta strand

272 – 275

4

Turn

289 – 292

4

Helix

311 – 320

10

Beta strand

330 – 332

3

Turn

366 – 368

3

Sequences

	Sequence		Length	Mass (Da)
	Q38945-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: CC0B3F3487859EC8	FASTA	373	39,374
	10 20 30 40 50 60 MAVDSLETEI DTAVRVVHLA SSLCVKVQEK LHLPNGGHVK SKDDDSPVTV ADFGVQAIVS 70 80 90 100 110 120 WVLAEVFGDQ NLSIVAEEDT ETLSEADSLG LLGAVSNAVN EALSEAQNYG LPKPVKPLGS 130 140 150 160 170 180 SEILKAISRC NSVGGPKGRH WVLDPVDGTL GFVRGDQYAV ALALIENGKV LLGVLGCPNY 190 200 210 220 230 240 PVKKECLSNG CNQAMKTKAV AGSVSKGCVM YAKRGSGQAW MQPLIVGGIP ESATLLKVSS 250 260 270 280 290 300 VDDPVLATVC EPVERANSNH LFTAGLANSM GVRKQPMRVY SMVKYAAIAR GDAEVFMKFA 310 320 330 340 350 360 QSSYKEKIWD HAAGVVIVEE AGGVVTDAGG RNLDFSKGVY LEGLDRGIIA CSGQVLHEKI 370 IGAVYASWES SSL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A cDNA clone encoding Arabidopsis HAL2-like (AHL) protein." Cheong J.-J., Kwon H.-B., Goodman H.M. Plant Gene Register PGR96-042 Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Arabidopsis Ahl encodes a PAP-specific phosphatase that is sensitive to toxic metal ions." Cheong J.-J., Kwon H.-B., Goodman H.M. Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Landsberg erecta.
[3]	"The Arabidopsis HAL2-like gene family includes a novel sodium-sensitive phosphatase." Gil-Mascarell R., Lopez-Coronado J.M., Belles J.M., Serrano R., Rodriguez P.L. Plant J. 17:373-383(1999) [PubMed: 10205895] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION. Strain: cv. Columbia.
[4]	"Structural analysis of Arabidopsis thaliana chromosome 5. XI." Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H., Tabata S. Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]	"Theoretical structure of Arabidopsis thaliana PAP-specific phosphatase (AHL)." Lim Y., Jung J. Submitted (AUG-2000) to the PDB data bank Cited for: 3D-STRUCTURE MODELING.

Cross-references

Sequence databases

EMBLGenBankDDBJ

U55205 mRNA. Translation: AAB52964.1.
AF016644 Genomic DNA. Translation: AAB94051.1.
AB026634 Genomic DNA. Translation: BAA97512.1.
AY045848 mRNA. Translation: AAK76522.1.
AY091377 mRNA. Translation: AAM14316.1.
AY086488 mRNA. Translation: AAM63490.1.

IPI

IPI00540815.

RefSeq

NP_200250.1.

UniGene

At.9004

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1FLF	model	-	A	1-373	[»]
1Q00	model	-	A	1-373	[»]

ModBase

Search...

Proteomic databases

PRIDE

Q38945.

Genome annotation databases

GeneID

835527.

GenomeReviews

Gene locus AT5G54390 in contig BA000015_GR.

KEGG

ath:AT5G54390.

NMPDR

fig|3702.1.peg.27347.

Organism-specific databases

GeneFarm

2322.

TAIR

At5g54390.

Phylogenomic databases

OMA

Q38945. IFMKFAR.

Enzyme and pathway databases

BRENDA

3.1.3.7. 302.

Gene expression databases

ArrayExpress

Q38945.

GermOnline

AT5G54390. Arabidopsis thaliana.

Family and domain databases

InterPro

IPR006239. Bisphos_HAL2.
IPR000760. Inositol_P.
[Graphical view]

PANTHER

PTHR20854. Inositol_P. 1 hit.

Pfam

PF00459. Inositol_P. 1 hit.
[Graphical view]

PRINTS

PR00378. INOSPHPHTASE.

TIGRFAMs

TIGR01330. bisphos_HAL2. 1 hit.

PROSITE

PS00629. IMP_1. 1 hit.
PS00630. IMP_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

DPNPH_ARATH

Accession

Primary (citable) accession number: Q38945
Secondary accession number(s): O48892

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 29, 2003
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)