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Protein

Steroid 5-alpha-reductase DET2

Gene

DET2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in a reduction step in the biosynthesis of the plant steroid, brassinolide; acts at the second step in brassinolide biosynthesis in the 5alpha-reduction of (24R)- 24-methylcholest-4-en-3-one, which is further modified to form campestanol. Can use progesterone, testosterone, androstenedione and campestenone as substrate.6 Publications

Catalytic activityi

A 3-oxo-5-alpha-steroid + NADP+ = a 3-oxo-Delta(4)-steroid + NADPH.3 Publications

Enzyme regulationi

Inhibited by the 4-azasteroids 4-MA.1 Publication

Kineticsi

  1. KM=2.5 µM for testosterone1 Publication
  2. KM=0.4 µM for progesterone1 Publication
  1. Vmax=0.2 nmol/min/mg enzyme with testosterone as substrate1 Publication
  2. Vmax=0.5 nmol/min/mg enzyme with progesterone as substrate1 Publication

pH dependencei

Optimum pH is 6.8.1 Publication

Pathway:ibrassinosteroid biosynthesis

This protein is involved in the pathway brassinosteroid biosynthesis, which is part of Plant hormone biosynthesis.
View all proteins of this organism that are known to be involved in the pathway brassinosteroid biosynthesis and in Plant hormone biosynthesis.

GO - Molecular functioni

GO - Biological processi

  • brassinosteroid biosynthetic process Source: TAIR
  • brassinosteroid homeostasis Source: TAIR
  • oxidation-reduction process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Brassinosteroid biosynthesis, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciARA:AT2G38050-MONOMER.
MetaCyc:AT2G38050-MONOMER.
UniPathwayiUPA00381.

Names & Taxonomyi

Protein namesi
Recommended name:
Steroid 5-alpha-reductase DET2 (EC:1.3.1.22)
Short name:
AtDET2
Alternative name(s):
Protein COMPACT ROSETTE 1
Protein DEETIOLATED 2
Gene namesi
Name:DET2
Synonyms:CRO1
Ordered Locus Names:At2g38050
ORF Names:T8P21.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G38050.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei13 – 3321HelicalSequence AnalysisAdd
BLAST
Transmembranei51 – 7121HelicalSequence AnalysisAdd
BLAST
Transmembranei79 – 9921HelicalSequence AnalysisAdd
BLAST
Transmembranei113 – 13321HelicalSequence AnalysisAdd
BLAST
Transmembranei148 – 16821HelicalSequence AnalysisAdd
BLAST
Transmembranei205 – 22521HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Brassinolide-reversible dark- and light-grown defects; small dark-green dwarf plants with a limited leaves expension due to a reduced number of cells per leaf blade.4 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi204 – 2041E → K in cro1-2 and det2-1; loss of activity, enhanced sensitivity to abscisic acid (ABA) during seed germination. 6 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 262262Steroid 5-alpha-reductase DET2PRO_0000213681Add
BLAST

Proteomic databases

PaxDbiQ38944.
PRIDEiQ38944.

Interactioni

Protein-protein interaction databases

BioGridi3727. 1 interaction.
IntActiQ38944. 1 interaction.
STRINGi3702.AT2G38050.1.

Structurei

3D structure databases

ProteinModelPortaliQ38944.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the steroid 5-alpha reductase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG82739.
HOGENOMiHOG000050133.
InParanoidiQ38944.
KOiK09591.
OMAiRGWFFPL.
PhylomeDBiQ38944.

Family and domain databases

InterProiIPR016636. 3-oxo-5-alpha-steroid_4-DH.
IPR001104. 3-oxo-5_a-steroid_4-DH_C.
[Graphical view]
PfamiPF02544. Steroid_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF015596. 5_alpha-SR2. 1 hit.
PROSITEiPS50244. S5A_REDUCTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q38944-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEIADKTFF RYCLLTLIFA GPPTAVLLKF LQAPYGKHNR TGWGPTVSPP
60 70 80 90 100
IAWFVMESPT LWLTLLLFPF GRHALNPKSL LLFSPYLIHY FHRTIIYPLR
110 120 130 140 150
LFRSSFPAGK NGFPITIAAL AFTFNLLNGY IQARWVSHYK DDYEDGNWFW
160 170 180 190 200
WRFVIGMVVF ITGMYINITS DRTLVRLKKE NRGGYVIPRG GWFELVSCPN
210 220 230 240 250
YFGEAIEWLG WAVMTWSWAG IGFFLYTCSN LFPRARASHK WYIAKFKEEY
260
PKTRKAVIPF VY
Length:262
Mass (Da):30,635
Last modified:April 16, 2002 - v2
Checksum:i88291B8A8AF55664
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti198 – 1981C → R in AAC49264 (PubMed:8602526).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53860 mRNA. Translation: AAC49264.1.
CP002685 Genomic DNA. Translation: AEC09484.1.
AY045926 mRNA. Translation: AAK76600.1.
AY079337 mRNA. Translation: AAL85068.1.
PIRiC84800.
RefSeqiNP_181340.1. NM_129361.4.
UniGeneiAt.22739.
At.66437.
At.74183.

Genome annotation databases

EnsemblPlantsiAT2G38050.1; AT2G38050.1; AT2G38050.
GeneIDi818383.
KEGGiath:AT2G38050.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53860 mRNA. Translation: AAC49264.1.
CP002685 Genomic DNA. Translation: AEC09484.1.
AY045926 mRNA. Translation: AAK76600.1.
AY079337 mRNA. Translation: AAL85068.1.
PIRiC84800.
RefSeqiNP_181340.1. NM_129361.4.
UniGeneiAt.22739.
At.66437.
At.74183.

3D structure databases

ProteinModelPortaliQ38944.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi3727. 1 interaction.
IntActiQ38944. 1 interaction.
STRINGi3702.AT2G38050.1.

Proteomic databases

PaxDbiQ38944.
PRIDEiQ38944.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G38050.1; AT2G38050.1; AT2G38050.
GeneIDi818383.
KEGGiath:AT2G38050.

Organism-specific databases

TAIRiAT2G38050.

Phylogenomic databases

eggNOGiNOG82739.
HOGENOMiHOG000050133.
InParanoidiQ38944.
KOiK09591.
OMAiRGWFFPL.
PhylomeDBiQ38944.

Enzyme and pathway databases

UniPathwayiUPA00381.
BioCyciARA:AT2G38050-MONOMER.
MetaCyc:AT2G38050-MONOMER.

Miscellaneous databases

PROiQ38944.

Family and domain databases

InterProiIPR016636. 3-oxo-5-alpha-steroid_4-DH.
IPR001104. 3-oxo-5_a-steroid_4-DH_C.
[Graphical view]
PfamiPF02544. Steroid_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF015596. 5_alpha-SR2. 1 hit.
PROSITEiPS50244. S5A_REDUCTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A role for brassinosteroids in light-dependent development of Arabidopsis."
    Li J., Nagpal P., Vitart V., McMorris T.C., Chory J.
    Science 272:398-401(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE.
    Strain: cv. Columbia.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "The Arabidopsis deetiolated2 mutant is blocked early in brassinosteroid biosynthesis."
    Fujioka S., Li J., Choi Y.-H., Seto H., Takatsuto S., Noguchi T., Watanabe T., Kuriyama H., Yokota T., Chory J., Sakurai A.
    Plant Cell 9:1951-1962(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF GLU-204.
    Strain: cv. Columbia.
  6. "Conservation of function between mammalian and plant steroid 5alpha-reductases."
    Li J., Biswas M.G., Chao A., Russell D.W., Chory J.
    Proc. Natl. Acad. Sci. U.S.A. 94:3554-3559(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF GLU-204, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION.
    Strain: cv. Columbia.
  7. "Genetic analysis of leaf form mutants from the Arabidopsis information service collection."
    Serrano-Cartagena J., Robles P., Ponce M.R., Micol J.L.
    Mol. Gen. Genet. 261:725-739(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, MUTAGENESIS OF GLU-204.
  8. "Arabidopsis det2 is defective in the conversion of (24R)-24-methylcholest-4-en-3-one to (24R)-24-methyl-5alpha-cholestan-3-one in brassinosteroid biosynthesis."
    Noguchi T., Fujioka S., Takatsuto S., Sakurai A., Yoshida S., Li J., Chory J.
    Plant Physiol. 120:833-840(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF GLU-204, CATALYTIC ACTIVITY.
    Strain: cv. Columbia.
  9. "A role for brassinosteroids in germination in Arabidopsis."
    Steber C.M., McCourt P.
    Plant Physiol. 125:763-769(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF GLU-204.
  10. "Brassinosteroids control the proliferation of leaf cells of Arabidopsis thaliana."
    Nakaya M., Tsukaya H., Murakami N., Kato M.
    Plant Cell Physiol. 43:239-244(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    Strain: cv. Columbia.
  11. "5alpha-Reductase activity in Lycopersicon esculentum: cloning and functional characterization of LeDET2 and evidence of the presence of two isoenzymes."
    Rosati F., Bardazzi I., De Blasi P., Simi L., Scarpi D., Guarna A., Serio M., Racchi M.L., Danza G.
    J. Steroid Biochem. Mol. Biol. 96:287-299(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, MUTAGENESIS OF GLU-204.
    Strain: cv. Columbia.

Entry informationi

Entry nameiDET2_ARATH
AccessioniPrimary (citable) accession number: Q38944
Secondary accession number(s): Q9SH83
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2002
Last sequence update: April 16, 2002
Last modified: July 22, 2015
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.