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Q38944 (DET2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Steroid 5-alpha-reductase DET2

Short name=AtDET2
EC=1.3.1.22
Alternative name(s):
Protein COMPACT ROSETTE 1
Protein DEETIOLATED 2
Gene names
Name:DET2
Synonyms:CRO1
Ordered Locus Names:At2g38050
ORF Names:T8P21.4
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length262 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in a reduction step in the biosynthesis of the plant steroid, brassinolide; acts at the second step in brassinolide biosynthesis in the 5alpha-reduction of (24R)- 24-methylcholest-4-en-3-one, which is further modified to form campestanol. Can use progesterone, testosterone, androstenedione and campestenone as substrate. Ref.1 Ref.5 Ref.6 Ref.8 Ref.10 Ref.11

Catalytic activity

A 3-oxo-5-alpha-steroid + NADP+ = a 3-oxo-Delta(4)-steroid + NADPH. Ref.6 Ref.8 Ref.11

Enzyme regulation

Inhibited by the 4-azasteroids 4-MA. Ref.6

Pathway

Plant hormone biosynthesis; brassinosteroid biosynthesis.

Subcellular location

Membrane; Multi-pass membrane protein By similarity.

Disruption phenotype

Brassinolide-reversible dark- and light-grown defects; small dark-green dwarf plants with a limited leaves expension due to a reduced number of cells per leaf blade. Ref.1 Ref.5 Ref.7 Ref.10

Sequence similarities

Belongs to the steroid 5-alpha reductase family.

Biophysicochemical properties

Kinetic parameters:

KM=2.5 µM for testosterone Ref.6

KM=0.4 µM for progesterone

Vmax=0.2 nmol/min/mg enzyme with testosterone as substrate

Vmax=0.5 nmol/min/mg enzyme with progesterone as substrate

pH dependence:

Optimum pH is 6.8.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 262262Steroid 5-alpha-reductase DET2
PRO_0000213681

Regions

Transmembrane13 – 3321Helical; Potential
Transmembrane51 – 7121Helical; Potential
Transmembrane79 – 9921Helical; Potential
Transmembrane113 – 13321Helical; Potential
Transmembrane148 – 16821Helical; Potential
Transmembrane205 – 22521Helical; Potential

Experimental info

Mutagenesis2041E → K in cro1-2 and det2-1; loss of activity, enhanced sensitivity to abscisic acid (ABA) during seed germination. Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.11
Sequence conflict1981C → R in AAC49264. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q38944 [UniParc].

Last modified April 16, 2002. Version 2.
Checksum: 88291B8A8AF55664

FASTA26230,635
        10         20         30         40         50         60 
MEEIADKTFF RYCLLTLIFA GPPTAVLLKF LQAPYGKHNR TGWGPTVSPP IAWFVMESPT 

        70         80         90        100        110        120 
LWLTLLLFPF GRHALNPKSL LLFSPYLIHY FHRTIIYPLR LFRSSFPAGK NGFPITIAAL 

       130        140        150        160        170        180 
AFTFNLLNGY IQARWVSHYK DDYEDGNWFW WRFVIGMVVF ITGMYINITS DRTLVRLKKE 

       190        200        210        220        230        240 
NRGGYVIPRG GWFELVSCPN YFGEAIEWLG WAVMTWSWAG IGFFLYTCSN LFPRARASHK 

       250        260 
WYIAKFKEEY PKTRKAVIPF VY 

« Hide

References

« Hide 'large scale' references
[1]"A role for brassinosteroids in light-dependent development of Arabidopsis."
Li J., Nagpal P., Vitart V., McMorris T.C., Chory J.
Science 272:398-401(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE.
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"The Arabidopsis deetiolated2 mutant is blocked early in brassinosteroid biosynthesis."
Fujioka S., Li J., Choi Y.-H., Seto H., Takatsuto S., Noguchi T., Watanabe T., Kuriyama H., Yokota T., Chory J., Sakurai A.
Plant Cell 9:1951-1962(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF GLU-204.
Strain: cv. Columbia.
[6]"Conservation of function between mammalian and plant steroid 5alpha-reductases."
Li J., Biswas M.G., Chao A., Russell D.W., Chory J.
Proc. Natl. Acad. Sci. U.S.A. 94:3554-3559(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF GLU-204, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION.
Strain: cv. Columbia.
[7]"Genetic analysis of leaf form mutants from the Arabidopsis information service collection."
Serrano-Cartagena J., Robles P., Ponce M.R., Micol J.L.
Mol. Gen. Genet. 261:725-739(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE, MUTAGENESIS OF GLU-204.
[8]"Arabidopsis det2 is defective in the conversion of (24R)-24-methylcholest-4-en-3-one to (24R)-24-methyl-5alpha-cholestan-3-one in brassinosteroid biosynthesis."
Noguchi T., Fujioka S., Takatsuto S., Sakurai A., Yoshida S., Li J., Chory J.
Plant Physiol. 120:833-840(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF GLU-204, CATALYTIC ACTIVITY.
Strain: cv. Columbia.
[9]"A role for brassinosteroids in germination in Arabidopsis."
Steber C.M., McCourt P.
Plant Physiol. 125:763-769(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF GLU-204.
[10]"Brassinosteroids control the proliferation of leaf cells of Arabidopsis thaliana."
Nakaya M., Tsukaya H., Murakami N., Kato M.
Plant Cell Physiol. 43:239-244(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
Strain: cv. Columbia.
[11]"5alpha-Reductase activity in Lycopersicon esculentum: cloning and functional characterization of LeDET2 and evidence of the presence of two isoenzymes."
Rosati F., Bardazzi I., De Blasi P., Simi L., Scarpi D., Guarna A., Serio M., Racchi M.L., Danza G.
J. Steroid Biochem. Mol. Biol. 96:287-299(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, MUTAGENESIS OF GLU-204.
Strain: cv. Columbia.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U53860 mRNA. Translation: AAC49264.1.
CP002685 Genomic DNA. Translation: AEC09484.1.
AY045926 mRNA. Translation: AAK76600.1.
AY079337 mRNA. Translation: AAL85068.1.
PIRC84800.
RefSeqNP_181340.1. NM_129361.4.
UniGeneAt.22739.
At.66437.
At.74183.

3D structure databases

ProteinModelPortalQ38944.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid3727. 1 interaction.
IntActQ38944. 1 interaction.
STRING3702.AT2G38050.1-P.

Proteomic databases

PaxDbQ38944.
PRIDEQ38944.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G38050.1; AT2G38050.1; AT2G38050.
GeneID818383.
KEGGath:AT2G38050.

Organism-specific databases

TAIRAT2G38050.

Phylogenomic databases

eggNOGNOG82739.
HOGENOMHOG000050133.
InParanoidQ38944.
KOK09591.
OMAVSAANYF.
PhylomeDBQ38944.
ProtClustDBPLN02392.

Enzyme and pathway databases

BioCycARA:AT2G38050-MONOMER.
MetaCyc:AT2G38050-MONOMER.
UniPathwayUPA00381.

Gene expression databases

GenevestigatorQ38944.

Family and domain databases

InterProIPR016636. 3-oxo-5-alpha-steroid_4-DH.
IPR001104. 3-oxo-5_a-steroid_4-DH_C.
[Graphical view]
PfamPF02544. Steroid_dh. 1 hit.
[Graphical view]
PIRSFPIRSF015596. 5_alpha-SR2. 1 hit.
PROSITEPS50244. S5A_REDUCTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ38944.

Entry information

Entry nameDET2_ARATH
AccessionPrimary (citable) accession number: Q38944
Secondary accession number(s): Q9SH83
Entry history
Integrated into UniProtKB/Swiss-Prot: April 16, 2002
Last sequence update: April 16, 2002
Last modified: April 16, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names