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Q38944

- DET2_ARATH

UniProt

Q38944 - DET2_ARATH

Protein

Steroid 5-alpha-reductase DET2

Gene

DET2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 2 (16 Apr 2002)
      Previous versions | rss
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    Functioni

    Involved in a reduction step in the biosynthesis of the plant steroid, brassinolide; acts at the second step in brassinolide biosynthesis in the 5alpha-reduction of (24R)- 24-methylcholest-4-en-3-one, which is further modified to form campestanol. Can use progesterone, testosterone, androstenedione and campestenone as substrate.6 Publications

    Catalytic activityi

    A 3-oxo-5-alpha-steroid + NADP+ = a 3-oxo-Delta(4)-steroid + NADPH.3 Publications

    Enzyme regulationi

    Inhibited by the 4-azasteroids 4-MA.1 Publication

    Kineticsi

    1. KM=2.5 µM for testosterone1 Publication
    2. KM=0.4 µM for progesterone1 Publication

    Vmax=0.2 nmol/min/mg enzyme with testosterone as substrate1 Publication

    Vmax=0.5 nmol/min/mg enzyme with progesterone as substrate1 Publication

    pH dependencei

    Optimum pH is 6.8.1 Publication

    Pathwayi

    GO - Molecular functioni

    1. 3-oxo-5-alpha-steroid 4-dehydrogenase activity Source: InterPro
    2. cholestenone 5-alpha-reductase activity Source: UniProtKB-EC
    3. progesterone 5-alpha-reductase activity Source: UniProtKB
    4. sterol 5-alpha reductase activity Source: TAIR

    GO - Biological processi

    1. brassinosteroid biosynthetic process Source: TAIR
    2. brassinosteroid homeostasis Source: TAIR
    3. oxidation-reduction process Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Brassinosteroid biosynthesis, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BioCyciARA:AT2G38050-MONOMER.
    MetaCyc:AT2G38050-MONOMER.
    UniPathwayiUPA00381.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Steroid 5-alpha-reductase DET2 (EC:1.3.1.22)
    Short name:
    AtDET2
    Alternative name(s):
    Protein COMPACT ROSETTE 1
    Protein DEETIOLATED 2
    Gene namesi
    Name:DET2
    Synonyms:CRO1
    Ordered Locus Names:At2g38050
    ORF Names:T8P21.4
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G38050.

    Subcellular locationi

    Membrane By similarity; Multi-pass membrane protein By similarity

    GO - Cellular componenti

    1. cytoplasm Source: InterPro
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Brassinolide-reversible dark- and light-grown defects; small dark-green dwarf plants with a limited leaves expension due to a reduced number of cells per leaf blade.4 Publications

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi204 – 2041E → K in cro1-2 and det2-1; loss of activity, enhanced sensitivity to abscisic acid (ABA) during seed germination. 6 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 262262Steroid 5-alpha-reductase DET2PRO_0000213681Add
    BLAST

    Proteomic databases

    PaxDbiQ38944.
    PRIDEiQ38944.

    Expressioni

    Gene expression databases

    GenevestigatoriQ38944.

    Interactioni

    Protein-protein interaction databases

    BioGridi3727. 1 interaction.
    IntActiQ38944. 1 interaction.
    STRINGi3702.AT2G38050.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ38944.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei13 – 3321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei51 – 7121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei79 – 9921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei113 – 13321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei148 – 16821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei205 – 22521HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the steroid 5-alpha reductase family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG82739.
    HOGENOMiHOG000050133.
    InParanoidiQ38944.
    KOiK09591.
    OMAiVSAANYF.
    PhylomeDBiQ38944.

    Family and domain databases

    InterProiIPR016636. 3-oxo-5-alpha-steroid_4-DH.
    IPR001104. 3-oxo-5_a-steroid_4-DH_C.
    [Graphical view]
    PfamiPF02544. Steroid_dh. 1 hit.
    [Graphical view]
    PIRSFiPIRSF015596. 5_alpha-SR2. 1 hit.
    PROSITEiPS50244. S5A_REDUCTASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q38944-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEEIADKTFF RYCLLTLIFA GPPTAVLLKF LQAPYGKHNR TGWGPTVSPP    50
    IAWFVMESPT LWLTLLLFPF GRHALNPKSL LLFSPYLIHY FHRTIIYPLR 100
    LFRSSFPAGK NGFPITIAAL AFTFNLLNGY IQARWVSHYK DDYEDGNWFW 150
    WRFVIGMVVF ITGMYINITS DRTLVRLKKE NRGGYVIPRG GWFELVSCPN 200
    YFGEAIEWLG WAVMTWSWAG IGFFLYTCSN LFPRARASHK WYIAKFKEEY 250
    PKTRKAVIPF VY 262
    Length:262
    Mass (Da):30,635
    Last modified:April 16, 2002 - v2
    Checksum:i88291B8A8AF55664
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti198 – 1981C → R in AAC49264. (PubMed:8602526)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U53860 mRNA. Translation: AAC49264.1.
    CP002685 Genomic DNA. Translation: AEC09484.1.
    AY045926 mRNA. Translation: AAK76600.1.
    AY079337 mRNA. Translation: AAL85068.1.
    PIRiC84800.
    RefSeqiNP_181340.1. NM_129361.4.
    UniGeneiAt.22739.
    At.66437.
    At.74183.

    Genome annotation databases

    EnsemblPlantsiAT2G38050.1; AT2G38050.1; AT2G38050.
    GeneIDi818383.
    KEGGiath:AT2G38050.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U53860 mRNA. Translation: AAC49264.1 .
    CP002685 Genomic DNA. Translation: AEC09484.1 .
    AY045926 mRNA. Translation: AAK76600.1 .
    AY079337 mRNA. Translation: AAL85068.1 .
    PIRi C84800.
    RefSeqi NP_181340.1. NM_129361.4.
    UniGenei At.22739.
    At.66437.
    At.74183.

    3D structure databases

    ProteinModelPortali Q38944.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 3727. 1 interaction.
    IntActi Q38944. 1 interaction.
    STRINGi 3702.AT2G38050.1-P.

    Proteomic databases

    PaxDbi Q38944.
    PRIDEi Q38944.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G38050.1 ; AT2G38050.1 ; AT2G38050 .
    GeneIDi 818383.
    KEGGi ath:AT2G38050.

    Organism-specific databases

    TAIRi AT2G38050.

    Phylogenomic databases

    eggNOGi NOG82739.
    HOGENOMi HOG000050133.
    InParanoidi Q38944.
    KOi K09591.
    OMAi VSAANYF.
    PhylomeDBi Q38944.

    Enzyme and pathway databases

    UniPathwayi UPA00381 .
    BioCyci ARA:AT2G38050-MONOMER.
    MetaCyc:AT2G38050-MONOMER.

    Miscellaneous databases

    PROi Q38944.

    Gene expression databases

    Genevestigatori Q38944.

    Family and domain databases

    InterProi IPR016636. 3-oxo-5-alpha-steroid_4-DH.
    IPR001104. 3-oxo-5_a-steroid_4-DH_C.
    [Graphical view ]
    Pfami PF02544. Steroid_dh. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF015596. 5_alpha-SR2. 1 hit.
    PROSITEi PS50244. S5A_REDUCTASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A role for brassinosteroids in light-dependent development of Arabidopsis."
      Li J., Nagpal P., Vitart V., McMorris T.C., Chory J.
      Science 272:398-401(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE.
      Strain: cv. Columbia.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "The Arabidopsis deetiolated2 mutant is blocked early in brassinosteroid biosynthesis."
      Fujioka S., Li J., Choi Y.-H., Seto H., Takatsuto S., Noguchi T., Watanabe T., Kuriyama H., Yokota T., Chory J., Sakurai A.
      Plant Cell 9:1951-1962(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF GLU-204.
      Strain: cv. Columbia.
    6. "Conservation of function between mammalian and plant steroid 5alpha-reductases."
      Li J., Biswas M.G., Chao A., Russell D.W., Chory J.
      Proc. Natl. Acad. Sci. U.S.A. 94:3554-3559(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF GLU-204, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION.
      Strain: cv. Columbia.
    7. "Genetic analysis of leaf form mutants from the Arabidopsis information service collection."
      Serrano-Cartagena J., Robles P., Ponce M.R., Micol J.L.
      Mol. Gen. Genet. 261:725-739(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE, MUTAGENESIS OF GLU-204.
    8. "Arabidopsis det2 is defective in the conversion of (24R)-24-methylcholest-4-en-3-one to (24R)-24-methyl-5alpha-cholestan-3-one in brassinosteroid biosynthesis."
      Noguchi T., Fujioka S., Takatsuto S., Sakurai A., Yoshida S., Li J., Chory J.
      Plant Physiol. 120:833-840(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF GLU-204, CATALYTIC ACTIVITY.
      Strain: cv. Columbia.
    9. "A role for brassinosteroids in germination in Arabidopsis."
      Steber C.M., McCourt P.
      Plant Physiol. 125:763-769(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF GLU-204.
    10. "Brassinosteroids control the proliferation of leaf cells of Arabidopsis thaliana."
      Nakaya M., Tsukaya H., Murakami N., Kato M.
      Plant Cell Physiol. 43:239-244(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
      Strain: cv. Columbia.
    11. "5alpha-Reductase activity in Lycopersicon esculentum: cloning and functional characterization of LeDET2 and evidence of the presence of two isoenzymes."
      Rosati F., Bardazzi I., De Blasi P., Simi L., Scarpi D., Guarna A., Serio M., Racchi M.L., Danza G.
      J. Steroid Biochem. Mol. Biol. 96:287-299(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, MUTAGENESIS OF GLU-204.
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiDET2_ARATH
    AccessioniPrimary (citable) accession number: Q38944
    Secondary accession number(s): Q9SH83
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 16, 2002
    Last sequence update: April 16, 2002
    Last modified: October 1, 2014
    This is version 103 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3