Steroid 5-alpha-reductase DET2 - Arabidopsis thaliana (Mouse-ear cress)

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Q38944 (DET2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 95. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Steroid 5-alpha-reductase DET2
Short name=AtDET2
EC=1.3.1.22

Alternative name(s):
Protein COMPACT ROSETTE 1
Protein DEETIOLATED 2

Gene names

Name:	DET2
Synonyms:	CRO1
Ordered Locus Names:	At2g38050
ORF Names:	T8P21.4

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	262 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in a reduction step in the biosynthesis of the plant steroid, brassinolide; acts at the second step in brassinolide biosynthesis in the 5alpha-reduction of (24R)- 24-methylcholest-4-en-3-one, which is further modified to form campestanol. Can use progesterone, testosterone, androstenedione and campestenone as substrate. Ref.1 Ref.5 Ref.6 Ref.8 Ref.10 Ref.11
Catalytic activity	A 3-oxo-5-alpha-steroid + NADP⁺ = a 3-oxo-Delta(4)-steroid + NADPH. Ref.6 Ref.8 Ref.11
Enzyme regulation	Inhibited by the 4-azasteroids 4-MA. Ref.6
Pathway	Plant hormone biosynthesis; brassinosteroid biosynthesis.
Subcellular location	Membrane; Multi-pass membrane protein By similarity.
Disruption phenotype	Brassinolide-reversible dark- and light-grown defects; small dark-green dwarf plants with a limited leaves expension due to a reduced number of cells per leaf blade. Ref.1 Ref.5 Ref.7 Ref.10
Sequence similarities	Belongs to the steroid 5-alpha reductase family.
Biophysicochemical properties	Kinetic parameters: K_M=2.5 µM for testosterone Ref.6 K_M=0.4 µM for progesterone V_max=0.2 nmol/min/mg enzyme with testosterone as substrate V_max=0.5 nmol/min/mg enzyme with progesterone as substrate pH dependence: Optimum pH is 6.8.

Ontologies

Keywords
Biological process	Brassinosteroid biosynthesis Lipid biosynthesis Lipid metabolism Steroid biosynthesis
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	brassinosteroid biosynthetic process Inferred from direct assay Ref.8. Source: TAIR brassinosteroid homeostasis Inferred from expression pattern PubMed 15908602. Source: TAIR
Cellular_component	cytoplasm Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	3-oxo-5-alpha-steroid 4-dehydrogenase activity Inferred from electronic annotation. Source: InterPro cholestenone 5-alpha-reductase activity Inferred from electronic annotation. Source: EC progesterone 5-alpha-reductase activity Inferred from direct assay Ref.6. Source: UniProtKB sterol 5-alpha reductase activity Inferred from direct assay Ref.8. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 262

262

Steroid 5-alpha-reductase DET2

PRO_0000213681

Regions

Transmembrane

13 – 33

Helical; Potential

Transmembrane

51 – 71

Helical; Potential

Transmembrane

79 – 99

Helical; Potential

Transmembrane

113 – 133

Helical; Potential

Transmembrane

148 – 168

Helical; Potential

Transmembrane

205 – 225

Helical; Potential

Experimental info

Mutagenesis

204

E → K in cro1-2 and det2-1; loss of activity, enhanced sensitivity to abscisic acid (ABA) during seed germination. Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.11

Sequence conflict

198

C → R in AAC49264. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

Q38944 [UniParc].

Last modified April 16, 2002. Version 2.
Checksum: 88291B8A8AF55664
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FASTA

262

30,635

        10         20         30         40         50         60 
MEEIADKTFF RYCLLTLIFA GPPTAVLLKF LQAPYGKHNR TGWGPTVSPP IAWFVMESPT 

        70         80         90        100        110        120 
LWLTLLLFPF GRHALNPKSL LLFSPYLIHY FHRTIIYPLR LFRSSFPAGK NGFPITIAAL 

       130        140        150        160        170        180 
AFTFNLLNGY IQARWVSHYK DDYEDGNWFW WRFVIGMVVF ITGMYINITS DRTLVRLKKE 

       190        200        210        220        230        240 
NRGGYVIPRG GWFELVSCPN YFGEAIEWLG WAVMTWSWAG IGFFLYTCSN LFPRARASHK 

       250        260 
WYIAKFKEEY PKTRKAVIPF VY

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A role for brassinosteroids in light-dependent development of Arabidopsis." Li J., Nagpal P., Vitart V., McMorris T.C., Chory J. Science 272:398-401(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"The Arabidopsis deetiolated2 mutant is blocked early in brassinosteroid biosynthesis." Fujioka S., Li J., Choi Y.-H., Seto H., Takatsuto S., Noguchi T., Watanabe T., Kuriyama H., Yokota T., Chory J., Sakurai A. Plant Cell 9:1951-1962(1997) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF GLU-204. Strain: cv. Columbia.
[6]	"Conservation of function between mammalian and plant steroid 5alpha-reductases." Li J., Biswas M.G., Chao A., Russell D.W., Chory J. Proc. Natl. Acad. Sci. U.S.A. 94:3554-3559(1997) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF GLU-204, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION. Strain: cv. Columbia.
[7]	"Genetic analysis of leaf form mutants from the Arabidopsis information service collection." Serrano-Cartagena J., Robles P., Ponce M.R., Micol J.L. Mol. Gen. Genet. 261:725-739(1999) [PubMed] [Europe PMC] [Abstract] Cited for: DISRUPTION PHENOTYPE, MUTAGENESIS OF GLU-204.
[8]	"Arabidopsis det2 is defective in the conversion of (24R)-24-methylcholest-4-en-3-one to (24R)-24-methyl-5alpha-cholestan-3-one in brassinosteroid biosynthesis." Noguchi T., Fujioka S., Takatsuto S., Sakurai A., Yoshida S., Li J., Chory J. Plant Physiol. 120:833-840(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF GLU-204, CATALYTIC ACTIVITY. Strain: cv. Columbia.
[9]	"A role for brassinosteroids in germination in Arabidopsis." Steber C.M., McCourt P. Plant Physiol. 125:763-769(2001) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS OF GLU-204.
[10]	"Brassinosteroids control the proliferation of leaf cells of Arabidopsis thaliana." Nakaya M., Tsukaya H., Murakami N., Kato M. Plant Cell Physiol. 43:239-244(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE. Strain: cv. Columbia.
[11]	"5alpha-Reductase activity in Lycopersicon esculentum: cloning and functional characterization of LeDET2 and evidence of the presence of two isoenzymes." Rosati F., Bardazzi I., De Blasi P., Simi L., Scarpi D., Guarna A., Serio M., Racchi M.L., Danza G. J. Steroid Biochem. Mol. Biol. 96:287-299(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, MUTAGENESIS OF GLU-204. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U53860 mRNA. Translation: AAC49264.1. CP002685 Genomic DNA. Translation: AEC09484.1. AY045926 mRNA. Translation: AAK76600.1. AY079337 mRNA. Translation: AAL85068.1.
IPI	IPI00544182.
PIR	C84800.
RefSeq	NP_181340.1. NM_129361.4.
UniGene	At.22739. At.66437. At.74183.
3D structure databases
ProteinModelPortal	Q38944.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q38944. 1 interaction.
STRING	3702.AT2G38050.1-P.
Proteomic databases
PaxDb	Q38944.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G38050.1; AT2G38050.1; AT2G38050.
GeneID	818383.
KEGG	ath:AT2G38050.
Organism-specific databases
TAIR	At2g38050.
Phylogenomic databases
eggNOG	NOG82739.
HOGENOM	HOG000050133.
InParanoid	Q38944.
KO	K09591.
OMA	SAANYFG.
PhylomeDB	Q38944.
ProtClustDB	PLN02392.
Enzyme and pathway databases
BioCyc	ARA:AT2G38050-MONOMER. MetaCyc:AT2G38050-MONOMER.
UniPathway	UPA00381.
Gene expression databases
Genevestigator	Q38944.
GermOnline	AT2G38050. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR016636. 3-oxo-5-alpha-steroid_4-DH. IPR001104. 3-oxo-5_a-steroid_4-DH_C. [Graphical view]
Pfam	PF02544. Steroid_dh. 1 hit. [Graphical view]
PIRSF	PIRSF015596. 5_alpha-SR2. 1 hit.
PROSITE	PS50244. S5A_REDUCTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DET2_ARATH

Accession

Primary (citable) accession number: Q38944
Secondary accession number(s): Q9SH83

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 16, 2002
Last sequence update:	April 16, 2002
Last modified:	May 1, 2013
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents