Q38944 (DET2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable steroid reductase DET2
EC=1.3.99.-

Gene names

Name:	DET2
Ordered Locus Names:	At2g38050
ORF Names:	T8P21.4

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	262 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Probably involved in a reduction step in the biosynthesis of the plant steroid, brassinolide. Ref.1
Pathway	Plant hormone biosynthesis; brassinosteroid biosynthesis.
Subcellular location	Membrane; Multi-pass membrane protein By similarity.
Disruption phenotype	Defects in light-regulated development. Ref.1
Sequence similarities	Belongs to the steroid 5-alpha reductase family.

Ontologies

Keywords
Biological process	Brassinosteroid biosynthesis Lipid synthesis Steroid biosynthesis
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	brassinosteroid biosynthetic process Inferred from direct assay. Source: TAIR brassinosteroid homeostasis Inferred from expression pattern. Source: TAIR
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-oxo-5-alpha-steroid 4-dehydrogenase activity Inferred from electronic annotation. Source: InterPro sterol 5-alpha reductase activity Inferred from direct assay. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 262	262	Probable steroid reductase DET2		PRO_0000213681
Regions
	Transmembrane	13 – 33	21	Helical; Potential
	Transmembrane	51 – 71	21	Helical; Potential
	Transmembrane	113 – 133	21	Helical; Potential
	Transmembrane	148 – 168	21	Helical; Potential
	Transmembrane	205 – 225	21	Helical; Potential
Experimental info
	Sequence conflict	198	1	C → R in AAC49264. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q38944 [UniParc]. Last modified April 16, 2002. Version 2. Checksum: 88291B8A8AF55664	FASTA	262	30,635
	10 20 30 40 50 60 MEEIADKTFF RYCLLTLIFA GPPTAVLLKF LQAPYGKHNR TGWGPTVSPP IAWFVMESPT 70 80 90 100 110 120 LWLTLLLFPF GRHALNPKSL LLFSPYLIHY FHRTIIYPLR LFRSSFPAGK NGFPITIAAL 130 140 150 160 170 180 AFTFNLLNGY IQARWVSHYK DDYEDGNWFW WRFVIGMVVF ITGMYINITS DRTLVRLKKE 190 200 210 220 230 240 NRGGYVIPRG GWFELVSCPN YFGEAIEWLG WAVMTWSWAG IGFFLYTCSN LFPRARASHK 250 260 WYIAKFKEEY PKTRKAVIPF VY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A role for brassinosteroids in light-dependent development of Arabidopsis." Li J., Nagpal P., Vitart V., McMorris T.C., Chory J. Science 272:398-401(1996) [PubMed: 8602526] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U53860 mRNA. Translation: AAC49264.1. CP002685 Genomic DNA. Translation: AEC09484.1. AY045926 mRNA. Translation: AAK76600.1. AY079337 mRNA. Translation: AAL85068.1.
IPI	IPI00544182.
PIR	C84800.
RefSeq	NP_181340.1. NM_129361.4.
UniGene	At.22739. At.66437. At.74183.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q38944. 1 interaction.
STRING	Q38944.
Proteomic databases
PRIDE	Q38944.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G38050.1; AT2G38050.1; AT2G38050.
GeneID	818383.
GenomeReviews	Gene locus AT2G38050 in contig CT485783_GR.
KEGG	ath:AT2G38050.
NMPDR	fig|3702.1.peg.10913.
Organism-specific databases
TAIR	At2g38050.
Phylogenomic databases
eggNOG	KOG1638.
GeneTree	EPGT00070000029335.
HOGENOM	HBG714186.
InParanoid	Q38944.
OMA	SAANYFG.
PhylomeDB	Q38944.
ProtClustDB	PLN02392.
Enzyme and pathway databases
BioCyc	ARA:AT2G38050-MONOMER. MetaCyc:AT2G38050-MONOMER.
Gene expression databases
ArrayExpress	Q38944.
Genevestigator	Q38944.
GermOnline	AT2G38050. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR016636. 3-oxo-5-alpha-steroid_4-DH. IPR001104. 3-oxo-5_a-steroid_4-DH_C. [Graphical view]
KO	K09591.
Pfam	PF02544. Steroid_dh. 1 hit. [Graphical view]
PIRSF	PIRSF015596. 5_alpha-SR2. 1 hit.
PROSITE	PS50244. S5A_REDUCTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DET2_ARATH

Accession

Primary (citable) accession number: Q38944
Secondary accession number(s): Q9SH83

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 16, 2002
Last sequence update:	April 16, 2002
Last modified:	December 14, 2011
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program