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Protein

Rac-like GTP-binding protein ARAC5

Gene

ARAC5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in cell polarity control during the actin-dependent tip growth of root hairs.1 Publication
Inactive GDP-bound Rho GTPases reside in the cytosol, are found in a complex with Rho GDP-dissociation inhibitors (Rho GDIs), and are released from the GDI protein in order to translocate to membranes upon activation.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi16 – 21GTP6
Nucleotide bindingi119 – 121GTP3
Nucleotide bindingi159 – 161GTP3

GO - Molecular functioni

  • GTPase activity Source: TAIR
  • GTP binding Source: UniProtKB
  • protein kinase binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-194840. Rho GTPase cycle.
R-ATH-198203. PI3K/AKT activation.
R-ATH-392451. G beta:gamma signalling through PI3Kgamma.
R-ATH-5666185. RHO GTPases Activate Rhotekin and Rhophilins.
R-ATH-5689896. Ovarian tumor domain proteases.
R-ATH-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Rac-like GTP-binding protein ARAC5
Alternative name(s):
GTPase protein ROP4
Gene namesi
Name:ARAC5
Synonyms:ATGP3, ROP4
Ordered Locus Names:At1g75840
ORF Names:T4O12.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G75840.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • nucleolus Source: TAIR
  • nucleus Source: TAIR
  • phragmoplast Source: TAIR
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi65E → A: Strongly impairs GEF-dependent nucleotide exchange. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001989191 – 193Rac-like GTP-binding protein ARAC5Add BLAST193
PropeptideiPRO_0000227584194 – 196Removed in mature formSequence analysis3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei193Cysteine methyl esterSequence analysis1
Lipidationi193S-geranylgeranyl cysteineSequence analysis1

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Proteomic databases

PaxDbiQ38937.

Expressioni

Tissue specificityi

Ubiquitous. Preferentially expressed at the tip of root hairs.2 Publications

Gene expression databases

GenevisibleiQ38937. AT.

Interactioni

Subunit structurei

Interacts with GDI1 and ROPGEF8 homodimer (PubMed:10798620, PubMed:17218277, PubMed:19335195). Binds to SPK1 (PubMed:18308939).4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
GDI1Q9SFC62EBI-1751308,EBI-1751328

GO - Molecular functioni

  • protein kinase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi29136. 8 interactors.
DIPiDIP-29820N.
IntActiQ38937. 1 interactor.
STRINGi3702.AT1G75840.1.

Structurei

Secondary structure

1196
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 13Combined sources8
Helixi19 – 28Combined sources10
Beta strandi43 – 49Combined sources7
Beta strandi52 – 59Combined sources8
Turni62 – 65Combined sources4
Beta strandi79 – 86Combined sources8
Helixi90 – 98Combined sources9
Helixi100 – 107Combined sources8
Beta strandi113 – 118Combined sources6
Helixi120 – 122Combined sources3
Helixi126 – 131Combined sources6
Helixi140 – 149Combined sources10
Beta strandi155 – 157Combined sources3
Turni160 – 162Combined sources3
Helixi166 – 177Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2NTYX-ray3.10C/D1-180[»]
ProteinModelPortaliQ38937.
SMRiQ38937.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ38937.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi35 – 43Effector regionSequence analysis9

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi182 – 190Poly-Lys9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rho family.Curated

Phylogenomic databases

eggNOGiKOG0393. Eukaryota.
COG1100. LUCA.
HOGENOMiHOG000233974.
InParanoidiQ38937.
KOiK04392.
OMAiSSGGCNI.
OrthoDBiEOG09360LB1.
PhylomeDBiQ38937.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51420. RHO. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q38937-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSASRFIKCV TVGDGAVGKT CMLISYTSNT FPTDYVPTVF DNFSANVVVD
60 70 80 90 100
GNTVNLGLWD TAGQEDYNRL RPLSYRGADV FILAFSLISK ASYENVAKKW
110 120 130 140 150
IPELRHYAPG VPIILVGTKL DLRDDKQFFI DHPGAVPITT NQGEELKKLI
160 170 180 190
GSPIYIECSS KTQQNVKAVF DAAIKVVLQP PKQKKKKKNK NRCVFL
Length:196
Mass (Da):21,777
Last modified:November 1, 1997 - v1
Checksum:i631317F3DA441A35
GO

Sequence cautioni

The sequence AAF26755 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti22M → I in AAC78242 (PubMed:9765526).Curated1
Sequence conflicti88 – 89IS → YC in AAD00114 (PubMed:8843944).Curated2
Sequence conflicti95N → H in AAC78242 (PubMed:9765526).Curated1
Sequence conflicti189 – 195NKNRCVF → TKAQKACSI in AAD00114 (PubMed:8843944).Curated7

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U64920 mRNA. Translation: AAD00114.1.
U52350 mRNA. Translation: AAC49855.1.
AF031428 mRNA. Translation: AAC78242.1.
AF115472 Genomic DNA. Translation: AAF40244.1.
AC007396 Genomic DNA. Translation: AAF26755.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE35764.1.
AY062800 mRNA. Translation: AAL32878.1.
AY081600 mRNA. Translation: AAM10162.1.
PIRiT48865.
RefSeqiNP_177712.1. NM_106234.3.
UniGeneiAt.25499.
At.49319.

Genome annotation databases

EnsemblPlantsiAT1G75840.1; AT1G75840.1; AT1G75840.
GeneIDi843917.
GrameneiAT1G75840.1; AT1G75840.1; AT1G75840.
KEGGiath:AT1G75840.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U64920 mRNA. Translation: AAD00114.1.
U52350 mRNA. Translation: AAC49855.1.
AF031428 mRNA. Translation: AAC78242.1.
AF115472 Genomic DNA. Translation: AAF40244.1.
AC007396 Genomic DNA. Translation: AAF26755.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE35764.1.
AY062800 mRNA. Translation: AAL32878.1.
AY081600 mRNA. Translation: AAM10162.1.
PIRiT48865.
RefSeqiNP_177712.1. NM_106234.3.
UniGeneiAt.25499.
At.49319.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2NTYX-ray3.10C/D1-180[»]
ProteinModelPortaliQ38937.
SMRiQ38937.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi29136. 8 interactors.
DIPiDIP-29820N.
IntActiQ38937. 1 interactor.
STRINGi3702.AT1G75840.1.

Proteomic databases

PaxDbiQ38937.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G75840.1; AT1G75840.1; AT1G75840.
GeneIDi843917.
GrameneiAT1G75840.1; AT1G75840.1; AT1G75840.
KEGGiath:AT1G75840.

Organism-specific databases

TAIRiAT1G75840.

Phylogenomic databases

eggNOGiKOG0393. Eukaryota.
COG1100. LUCA.
HOGENOMiHOG000233974.
InParanoidiQ38937.
KOiK04392.
OMAiSSGGCNI.
OrthoDBiEOG09360LB1.
PhylomeDBiQ38937.

Enzyme and pathway databases

ReactomeiR-ATH-194840. Rho GTPase cycle.
R-ATH-198203. PI3K/AKT activation.
R-ATH-392451. G beta:gamma signalling through PI3Kgamma.
R-ATH-5666185. RHO GTPases Activate Rhotekin and Rhophilins.
R-ATH-5689896. Ovarian tumor domain proteases.
R-ATH-6798695. Neutrophil degranulation.

Miscellaneous databases

EvolutionaryTraceiQ38937.
PROiQ38937.

Gene expression databases

GenevisibleiQ38937. AT.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51420. RHO. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRAC5_ARATH
AccessioniPrimary (citable) accession number: Q38937
Secondary accession number(s): O48545, Q9LQT0, Q9ZRD5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 30, 2016
This is version 144 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.