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Protein

Fe(3+)-Zn(2+) purple acid phosphatase 12

Gene

PAP12

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactori

Protein has several cofactor binding sites:
  • Fe cationBy similarityNote: Binds 1 Fe cation per subunit.By similarity
  • Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi168IronBy similarity1
Metal bindingi197IronBy similarity1
Metal bindingi197ZincBy similarity1
Metal bindingi200IronBy similarity1
Metal bindingi234ZincBy similarity1
Binding sitei234SubstrateBy similarity1
Metal bindingi319ZincBy similarity1
Active sitei329Proton donorBy similarity1
Metal bindingi356ZincBy similarity1
Metal bindingi358IronBy similarity1

GO - Molecular functioni

  • acid phosphatase activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • cellular response to phosphate starvation Source: TAIR

Keywordsi

Molecular functionHydrolase
LigandIron, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Fe(3+)-Zn(2+) purple acid phosphatase 12 (EC:3.1.3.2)
Short name:
PAP
Alternative name(s):
Iron(III)-zinc(II) purple acid phosphatase 12
Gene namesi
Name:PAP12
Synonyms:At10, Ath1, PAP1
Ordered Locus Names:At2g27190
ORF Names:T22O13.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G27190
TAIRilocus:2005533 AT2G27190

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 28Sequence analysisAdd BLAST28
ChainiPRO_000002399329 – 469Fe(3+)-Zn(2+) purple acid phosphatase 12Add BLAST441

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi114N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi176N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi307N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi378InterchainBy similarity
Glycosylationi429N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ38924
PRIDEiQ38924

Expressioni

Tissue specificityi

Expressed in roots, stems, leaves, flowers and siliques.1 Publication

Inductioni

Sligthly by phosphate deprivation.1 Publication

Gene expression databases

ExpressionAtlasiQ38924 baseline and differential
GenevisibleiQ38924 AT

Interactioni

Subunit structurei

Homodimer; disulfide-linked.By similarity

Protein-protein interaction databases

STRINGi3702.AT2G27190.1

Structurei

3D structure databases

ProteinModelPortaliQ38924
SMRiQ38924
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni356 – 358Substrate bindingBy similarity3

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1378 Eukaryota
COG1409 LUCA
HOGENOMiHOG000238330
InParanoidiQ38924
KOiK22390
OMAiGLATEMT
OrthoDBiEOG093609CL

Family and domain databases

CDDicd00063 FN3, 1 hit
Gene3Di2.60.40.380, 1 hit
3.60.21.10, 1 hit
InterProiView protein in InterPro
IPR004843 Calcineurin-like_PHP_ApaH
IPR003961 FN3_dom
IPR029052 Metallo-depent_PP-like
IPR008963 Purple_acid_Pase-like_N
IPR015914 Purple_acid_Pase_N
IPR025733 Purple_acid_PPase_C_dom
PfamiView protein in Pfam
PF00149 Metallophos, 1 hit
PF14008 Metallophos_C, 1 hit
PF16656 Pur_ac_phosph_N, 1 hit
SUPFAMiSSF49363 SSF49363, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q38924-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSRSDLKIK RVSLIIFLLS VLVEFCYGGF TSEYVRGSDL PDDMPLDSDV
60 70 80 90 100
FEVPPGPNSP QQVHVTQGNH EGNGVIISWV TPVKPGSKTV QYWCENEKSR
110 120 130 140 150
KQAEATVNTY RFFNYTSGYI HHCLIDDLEF DTKYYYEIGS GKWSRRFWFF
160 170 180 190 200
IPPKSGPDVP YTFGLIGDLG QTYDSNSTLS HYEMNPGKGQ AVLFVGDLSY
210 220 230 240 250
ADRYPNHDNN RWDTWGRFVE RSVAYQPWIW TAGNHEIDFV PDIGEIEPFK
260 270 280 290 300
PFMNRYHTPH KASGSISPLW YSIKRASAYI IVMSCYSSYG IYTPQYKWLE
310 320 330 340 350
KELQGVNRTE TPWLIVLVHS PFYSSYVHHY MEGETLRVMY EQWFVKYKVD
360 370 380 390 400
VVFAGHVHAY ERSERVSNIA YNIVNGLCEP ISDESAPIYI TIGDGGNSEG
410 420 430 440 450
LLTDMMQPQP KYSAFREASF GHGLLEIKNR THAYFSWNRN QDGNAVAADS
460
VWLLNRFWRA QKKTWLDAF
Length:469
Mass (Da):54,123
Last modified:May 3, 2011 - v3
Checksum:i7B75A7A097DAEFAF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti57P → H in AAM15913 (PubMed:12021284).Curated1
Sequence conflicti57P → H in AAD26885 (PubMed:10617197).Curated1
Sequence conflicti91Q → R in AAA91803 (Ref. 2) Curated1
Sequence conflicti97E → K in AAA91803 (Ref. 2) Curated1
Sequence conflicti151I → T in AAA91803 (Ref. 2) Curated1
Sequence conflicti216G → R in CAA23388 (PubMed:8580968).Curated1
Sequence conflicti264G → R in CAA23388 (PubMed:8580968).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF492664 mRNA Translation: AAM15913.1
U48448 Genomic DNA Translation: AAA91803.1
AC007290 Genomic DNA Translation: AAD26885.1
CP002685 Genomic DNA Translation: AEC07951.1
AY065067 mRNA Translation: AAL57700.1
AY133599 mRNA Translation: AAM91429.1
F20043 mRNA Translation: CAA23388.1
PIRiH84669
RefSeqiNP_180287.2, NM_128277.5
UniGeneiAt.28647

Genome annotation databases

EnsemblPlantsiAT2G27190.1; AT2G27190.1; AT2G27190
GeneIDi817261
GrameneiAT2G27190.1; AT2G27190.1; AT2G27190
KEGGiath:AT2G27190

Similar proteinsi

Entry informationi

Entry nameiPPA12_ARATH
AccessioniPrimary (citable) accession number: Q38924
Secondary accession number(s): Q42349
, Q540Q7, Q8VZC6, Q9SHS9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 3, 2011
Last modified: May 23, 2018
This is version 148 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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