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Q38924

- PPA12_ARATH

UniProt

Q38924 - PPA12_ARATH

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Protein
Fe(3+)-Zn(2+) purple acid phosphatase 12
Gene
PAP12, At10, Ath1, PAP1, At2g27190, T22O13.4
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactori

Binds 1 iron ion per subunit By similarity.
Binds 1 zinc ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi168 – 1681Iron By similarity
Metal bindingi197 – 1971Iron By similarity
Metal bindingi197 – 1971Zinc By similarity
Metal bindingi200 – 2001Iron By similarity
Metal bindingi234 – 2341Zinc By similarity
Binding sitei234 – 2341Substrate By similarity
Metal bindingi319 – 3191Zinc By similarity
Active sitei329 – 3291Proton donor By similarity
Metal bindingi356 – 3561Zinc By similarity
Metal bindingi358 – 3581Iron By similarity

GO - Molecular functioni

  1. acid phosphatase activity Source: TAIR
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cellular response to phosphate starvation Source: TAIR
  2. dephosphorylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Iron, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Fe(3+)-Zn(2+) purple acid phosphatase 12 (EC:3.1.3.2)
Short name:
PAP
Alternative name(s):
Iron(III)-zinc(II) purple acid phosphatase 12
Gene namesi
Name:PAP12
Synonyms:At10, Ath1, PAP1
Ordered Locus Names:At2g27190
ORF Names:T22O13.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G27190.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: TAIR
  2. extracellular region Source: UniProtKB-SubCell
  3. plant-type cell wall Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2828 Reviewed prediction
Add
BLAST
Chaini29 – 469441Fe(3+)-Zn(2+) purple acid phosphatase 12
PRO_0000023993Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi114 – 1141N-linked (GlcNAc...) Reviewed prediction
Glycosylationi176 – 1761N-linked (GlcNAc...) Reviewed prediction
Glycosylationi307 – 3071N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi378 – 378Interchain By similarity
Glycosylationi429 – 4291N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ38924.
PRIDEiQ38924.

Expressioni

Tissue specificityi

Expressed in roots, stems, leaves, flowers and siliques.1 Publication

Inductioni

Sligthly by phosphate deprivation.1 Publication

Gene expression databases

ArrayExpressiQ38924.
GenevestigatoriQ38924.

Interactioni

Subunit structurei

Homodimer; disulfide-linked By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ38924.
SMRiQ38924. Positions 43-458.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni356 – 3583Substrate binding By similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1409.
HOGENOMiHOG000238330.
InParanoidiQ38924.
OMAiFVERNLA.

Family and domain databases

Gene3Di2.60.40.380. 1 hit.
3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR003961. Fibronectin_type3.
IPR029052. Metallo-depent_PP-like.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
IPR025733. Purple_acid_PPase_C_dom.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
PF14008. Metallophos_C. 1 hit.
[Graphical view]
SMARTiSM00060. FN3. 1 hit.
[Graphical view]
SUPFAMiSSF49363. SSF49363. 1 hit.
SSF56300. SSF56300. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q38924-1 [UniParc]FASTAAdd to Basket

« Hide

MSSRSDLKIK RVSLIIFLLS VLVEFCYGGF TSEYVRGSDL PDDMPLDSDV    50
FEVPPGPNSP QQVHVTQGNH EGNGVIISWV TPVKPGSKTV QYWCENEKSR 100
KQAEATVNTY RFFNYTSGYI HHCLIDDLEF DTKYYYEIGS GKWSRRFWFF 150
IPPKSGPDVP YTFGLIGDLG QTYDSNSTLS HYEMNPGKGQ AVLFVGDLSY 200
ADRYPNHDNN RWDTWGRFVE RSVAYQPWIW TAGNHEIDFV PDIGEIEPFK 250
PFMNRYHTPH KASGSISPLW YSIKRASAYI IVMSCYSSYG IYTPQYKWLE 300
KELQGVNRTE TPWLIVLVHS PFYSSYVHHY MEGETLRVMY EQWFVKYKVD 350
VVFAGHVHAY ERSERVSNIA YNIVNGLCEP ISDESAPIYI TIGDGGNSEG 400
LLTDMMQPQP KYSAFREASF GHGLLEIKNR THAYFSWNRN QDGNAVAADS 450
VWLLNRFWRA QKKTWLDAF 469
Length:469
Mass (Da):54,123
Last modified:May 3, 2011 - v3
Checksum:i7B75A7A097DAEFAF
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti57 – 571P → H in AAM15913. 1 Publication
Sequence conflicti57 – 571P → H in AAD26885. 1 Publication
Sequence conflicti91 – 911Q → R in AAA91803. 1 Publication
Sequence conflicti97 – 971E → K in AAA91803. 1 Publication
Sequence conflicti151 – 1511I → T in AAA91803. 1 Publication
Sequence conflicti216 – 2161G → R in CAA23388. 1 Publication
Sequence conflicti264 – 2641G → R in CAA23388. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF492664 mRNA. Translation: AAM15913.1.
U48448 Genomic DNA. Translation: AAA91803.1.
AC007290 Genomic DNA. Translation: AAD26885.1.
CP002685 Genomic DNA. Translation: AEC07951.1.
AY065067 mRNA. Translation: AAL57700.1.
AY133599 mRNA. Translation: AAM91429.1.
F20043 mRNA. Translation: CAA23388.1.
PIRiH84669.
RefSeqiNP_180287.2. NM_128277.5.
UniGeneiAt.28647.

Genome annotation databases

EnsemblPlantsiAT2G27190.1; AT2G27190.1; AT2G27190.
GeneIDi817261.
KEGGiath:AT2G27190.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF492664 mRNA. Translation: AAM15913.1 .
U48448 Genomic DNA. Translation: AAA91803.1 .
AC007290 Genomic DNA. Translation: AAD26885.1 .
CP002685 Genomic DNA. Translation: AEC07951.1 .
AY065067 mRNA. Translation: AAL57700.1 .
AY133599 mRNA. Translation: AAM91429.1 .
F20043 mRNA. Translation: CAA23388.1 .
PIRi H84669.
RefSeqi NP_180287.2. NM_128277.5.
UniGenei At.28647.

3D structure databases

ProteinModelPortali Q38924.
SMRi Q38924. Positions 43-458.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi Q38924.
PRIDEi Q38924.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G27190.1 ; AT2G27190.1 ; AT2G27190 .
GeneIDi 817261.
KEGGi ath:AT2G27190.

Organism-specific databases

TAIRi AT2G27190.

Phylogenomic databases

eggNOGi COG1409.
HOGENOMi HOG000238330.
InParanoidi Q38924.
OMAi FVERNLA.

Gene expression databases

ArrayExpressi Q38924.
Genevestigatori Q38924.

Family and domain databases

Gene3Di 2.60.40.380. 1 hit.
3.60.21.10. 1 hit.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR003961. Fibronectin_type3.
IPR029052. Metallo-depent_PP-like.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
IPR025733. Purple_acid_PPase_C_dom.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
PF14008. Metallophos_C. 1 hit.
[Graphical view ]
SMARTi SM00060. FN3. 1 hit.
[Graphical view ]
SUPFAMi SSF49363. SSF49363. 1 hit.
SSF56300. SSF56300. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Purple acid phosphatases of Arabidopsis thaliana. Comparative analysis and differential regulation by phosphate deprivation."
    Li D., Zhu H., Liu K., Liu X., Leggewie G., Udvardi M., Wang D.
    J. Biol. Chem. 277:27772-27781(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, GENE FAMILY, NOMENCLATURE.
    Strain: cv. Col-1.
  2. Patel K.S., Lockless S.W., McKnight T.D.
    Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Landsberg erecta.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 138-268.
    Strain: cv. Columbia.
  7. "Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower."
    Zhu H., Qian W., Lu X., Li D., Liu X., Liu K., Wang D.
    Plant Mol. Biol. 59:581-594(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiPPA12_ARATH
AccessioniPrimary (citable) accession number: Q38924
Secondary accession number(s): Q42349
, Q540Q7, Q8VZC6, Q9SHS9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 3, 2011
Last modified: June 11, 2014
This is version 121 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi