Reviewed, UniProtKB/Swiss-Prot Q38924 (PPA12_ARATH)

Names and origin

Protein names

Recommended name:
    Fe(3+)-Zn(2+) purple acid phosphatase 12
      Short name=PAP
    EC=3.1.3.2
Alternative name(s):
    Iron(III)-zinc(II) purple acid phosphatase 12

Gene names

Name:	PAP12
Synonyms:	At10, Ath1, PAP1
Ordered Locus Names:	At2g27190
ORF Names:	T22O13.4

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	469 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	A phosphate monoester + H2O = an alcohol + phosphate.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer; disulfide-linked By similarity.
Subcellular location	Secreted.
Tissue specificity	Expressed in roots, stems, leaves, flowers and siliques. Ref.6
Induction	Sligthly by phosphate deprivation. Ref.1
Sequence similarities	Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Iron Metal-binding Zinc
Molecular function	Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular response to phosphate starvation Inferred from expression pattern. Source: TAIR
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell plant-type cell wall Inferred from direct assay. Source: TAIR
Molecular function	acid phosphatase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	Potential
	Chain	29 – 469	441	Fe(3+)-Zn(2+) purple acid phosphatase 12		PRO_0000023993
Regions
	Region	356 – 358	3	Substrate binding By similarity
Sites
	Active site	329	1	Proton donor By similarity
	Metal binding	168	1	Iron By similarity
	Metal binding	197	1	Iron By similarity
	Metal binding	197	1	Zinc By similarity
	Metal binding	200	1	Iron By similarity
	Metal binding	234	1	Zinc By similarity
	Metal binding	319	1	Zinc By similarity
	Metal binding	356	1	Zinc By similarity
	Metal binding	358	1	Iron By similarity
	Binding site	234	1	Substrate By similarity
Amino acid modifications
	Glycosylation	114	1	N-linked (GlcNAc...) Potential
	Glycosylation	176	1	N-linked (GlcNAc...) Potential
	Glycosylation	307	1	N-linked (GlcNAc...) Potential
	Glycosylation	429	1	N-linked (GlcNAc...) Potential
	Disulfide bond	378		Interchain By similarity
Experimental info
	Sequence conflict	57	1	H → P in AAA91803. Ref.2
	Sequence conflict	57	1	H → P in AAL57700. Ref.4
	Sequence conflict	57	1	H → P in AAM91429. Ref.4
	Sequence conflict	91	1	Q → R in AAA91803. Ref.2
	Sequence conflict	97	1	E → K in AAA91803. Ref.2
	Sequence conflict	151	1	I → T in AAA91803. Ref.2
	Sequence conflict	216	1	G → R in CAA23388. Ref.5
	Sequence conflict	264	1	G → R in CAA23388. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q38924-1 [UniParc]. Last modified June 6, 2002. Version 2. Checksum: 21BFB941002B931B	FASTA	469	54,163
	10 20 30 40 50 60 MSSRSDLKIK RVSLIIFLLS VLVEFCYGGF TSEYVRGSDL PDDMPLDSDV FEVPPGHNSP 70 80 90 100 110 120 QQVHVTQGNH EGNGVIISWV TPVKPGSKTV QYWCENEKSR KQAEATVNTY RFFNYTSGYI 130 140 150 160 170 180 HHCLIDDLEF DTKYYYEIGS GKWSRRFWFF IPPKSGPDVP YTFGLIGDLG QTYDSNSTLS 190 200 210 220 230 240 HYEMNPGKGQ AVLFVGDLSY ADRYPNHDNN RWDTWGRFVE RSVAYQPWIW TAGNHEIDFV 250 260 270 280 290 300 PDIGEIEPFK PFMNRYHTPH KASGSISPLW YSIKRASAYI IVMSCYSSYG IYTPQYKWLE 310 320 330 340 350 360 KELQGVNRTE TPWLIVLVHS PFYSSYVHHY MEGETLRVMY EQWFVKYKVD VVFAGHVHAY 370 380 390 400 410 420 ERSERVSNIA YNIVNGLCEP ISDESAPIYI TIGDGGNSEG LLTDMMQPQP KYSAFREASF 430 440 450 460 GHGLLEIKNR THAYFSWNRN QDGNAVAADS VWLLNRFWRA QKKTWLDAF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Purple acid phosphatases of Arabidopsis thaliana. Comparative analysis and differential regulation by phosphate deprivation." Li D., Zhu H., Liu K., Liu X., Leggewie G., Udvardi M., Wang D. J. Biol. Chem. 277:27772-27781(2002) [PubMed: 12021284] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, GENE FAMILY, NOMENCLATURE. Strain: cv. Col-1.
[2]	Patel K.S., Lockless S.W., McKnight T.D. Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Landsberg erecta.
[3]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Further progress towards a catalogue of all Arabidopsis genes: analysis of a set of 5000 non-redundant ESTs." Cooke R., Raynal M., Laudie M., Grellet F., Delseny M., Morris P.-C., Guerrier D., Giraudat J., Quigley F., Clabault G., Li Y.-F., Mache R., Krivitzky M., Gy I.J.-J., Kreis M., Lecharny A., Parmentier Y., Marbach J. , Fleck J., Clement B., Philipps G., Herve C., Bardet C., Tremousaygue D., Lescure B., Lacomme C., Roby D., Jourjon M.-F., Chabrier P., Charpenteau J.-L., Desprez T., Amselem J., Chiapello H., Hoefte H. Plant J. 9:101-124(1996) [PubMed: 8580968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 138-268. Strain: cv. Columbia.
[6]	"Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower." Zhu H., Qian W., Lu X., Li D., Liu X., Liu K., Wang D. Plant Mol. Biol. 59:581-594(2005) [PubMed: 16244908] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF492664 mRNA. Translation: AAM15913.1. U48448 Genomic DNA. Translation: AAA91803.1. AC007290 Genomic DNA. Translation: AAD26885.1. AY065067 mRNA. Translation: AAL57700.1. AY133599 mRNA. Translation: AAM91429.1. F20043 mRNA. Translation: CAA23388.1.
IPI	IPI00540349.
PIR	H84669.
RefSeq	NP_180287.2.
UniGene	At.28647 Rsa.6200
3D structure databases
SMR	Q38924. Positions 43-458.
ModBase	Search...
Proteomic databases
PRIDE	Q38924.
Genome annotation databases
GeneID	817261.
GenomeReviews	Gene locus AT2G27190 in contig CT485783_GR.
KEGG	ath:AT2G27190.
NMPDR	fig|3702.1.peg.9750.
Organism-specific databases
TAIR	At2g27190.
Phylogenomic databases
eggNOG	KOG1378.
HOGENOM	HBG316723.
InParanoid	Q38924.
OMA	ISDESAP.
PhylomeDB	Q38924.
Enzyme and pathway databases
BRENDA	3.1.3.2. 302.
Gene expression databases
ArrayExpress	Q38924.
Genevestigator	Q38924.
GermOnline	AT2G27190. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR004843. M-pesterase. IPR008963. Purple_acid_Pase-like_N. IPR015914. Purple_acid_Pase_N. [Graphical view]
Gene3D	G3DSA:2.60.40.380. Purple_acid_Pase_N. 1 hit.
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PPA12_ARATH

Accession

Primary (citable) accession number: Q38924
Secondary accession number(s): Q42349 , Q540Q7, Q8VZC6, Q9SHS9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	June 6, 2002
Last modified:	February 9, 2010
This is version 88 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)