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Protein

Fe(3+)-Zn(2+) purple acid phosphatase 12

Gene

PAP12

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactori

Protein has several cofactor binding sites:
  • Fe cationBy similarityNote: Binds 1 Fe cation per subunit.By similarity
  • Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi168 – 1681IronBy similarity
Metal bindingi197 – 1971IronBy similarity
Metal bindingi197 – 1971ZincBy similarity
Metal bindingi200 – 2001IronBy similarity
Metal bindingi234 – 2341ZincBy similarity
Binding sitei234 – 2341SubstrateBy similarity
Metal bindingi319 – 3191ZincBy similarity
Active sitei329 – 3291Proton donorBy similarity
Metal bindingi356 – 3561ZincBy similarity
Metal bindingi358 – 3581IronBy similarity

GO - Molecular functioni

  • acid phosphatase activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • cellular response to phosphate starvation Source: TAIR
  • dephosphorylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Iron, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Fe(3+)-Zn(2+) purple acid phosphatase 12 (EC:3.1.3.2)
Short name:
PAP
Alternative name(s):
Iron(III)-zinc(II) purple acid phosphatase 12
Gene namesi
Name:PAP12
Synonyms:At10, Ath1, PAP1
Ordered Locus Names:At2g27190
ORF Names:T22O13.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G27190.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: TAIR
  • extracellular region Source: UniProtKB-SubCell
  • plant-type cell wall Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2828Sequence AnalysisAdd
BLAST
Chaini29 – 469441Fe(3+)-Zn(2+) purple acid phosphatase 12PRO_0000023993Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi114 – 1141N-linked (GlcNAc...)Sequence Analysis
Glycosylationi176 – 1761N-linked (GlcNAc...)Sequence Analysis
Glycosylationi307 – 3071N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi378 – 378InterchainBy similarity
Glycosylationi429 – 4291N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ38924.
PRIDEiQ38924.

Expressioni

Tissue specificityi

Expressed in roots, stems, leaves, flowers and siliques.1 Publication

Inductioni

Sligthly by phosphate deprivation.1 Publication

Interactioni

Subunit structurei

Homodimer; disulfide-linked.By similarity

Protein-protein interaction databases

STRINGi3702.AT2G27190.1.

Structurei

3D structure databases

ProteinModelPortaliQ38924.
SMRiQ38924. Positions 43-458.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni356 – 3583Substrate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1409.
HOGENOMiHOG000238330.
InParanoidiQ38924.
OMAiFMNRYHT.

Family and domain databases

Gene3Di2.60.40.380. 1 hit.
3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR003961. FN3_dom.
IPR029052. Metallo-depent_PP-like.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
IPR025733. Purple_acid_PPase_C_dom.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
PF14008. Metallophos_C. 1 hit.
[Graphical view]
SMARTiSM00060. FN3. 1 hit.
[Graphical view]
SUPFAMiSSF49363. SSF49363. 1 hit.
SSF56300. SSF56300. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q38924-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSRSDLKIK RVSLIIFLLS VLVEFCYGGF TSEYVRGSDL PDDMPLDSDV
60 70 80 90 100
FEVPPGPNSP QQVHVTQGNH EGNGVIISWV TPVKPGSKTV QYWCENEKSR
110 120 130 140 150
KQAEATVNTY RFFNYTSGYI HHCLIDDLEF DTKYYYEIGS GKWSRRFWFF
160 170 180 190 200
IPPKSGPDVP YTFGLIGDLG QTYDSNSTLS HYEMNPGKGQ AVLFVGDLSY
210 220 230 240 250
ADRYPNHDNN RWDTWGRFVE RSVAYQPWIW TAGNHEIDFV PDIGEIEPFK
260 270 280 290 300
PFMNRYHTPH KASGSISPLW YSIKRASAYI IVMSCYSSYG IYTPQYKWLE
310 320 330 340 350
KELQGVNRTE TPWLIVLVHS PFYSSYVHHY MEGETLRVMY EQWFVKYKVD
360 370 380 390 400
VVFAGHVHAY ERSERVSNIA YNIVNGLCEP ISDESAPIYI TIGDGGNSEG
410 420 430 440 450
LLTDMMQPQP KYSAFREASF GHGLLEIKNR THAYFSWNRN QDGNAVAADS
460
VWLLNRFWRA QKKTWLDAF
Length:469
Mass (Da):54,123
Last modified:May 3, 2011 - v3
Checksum:i7B75A7A097DAEFAF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti57 – 571P → H in AAM15913 (PubMed:12021284).Curated
Sequence conflicti57 – 571P → H in AAD26885 (PubMed:10617197).Curated
Sequence conflicti91 – 911Q → R in AAA91803 (Ref. 2) Curated
Sequence conflicti97 – 971E → K in AAA91803 (Ref. 2) Curated
Sequence conflicti151 – 1511I → T in AAA91803 (Ref. 2) Curated
Sequence conflicti216 – 2161G → R in CAA23388 (PubMed:8580968).Curated
Sequence conflicti264 – 2641G → R in CAA23388 (PubMed:8580968).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF492664 mRNA. Translation: AAM15913.1.
U48448 Genomic DNA. Translation: AAA91803.1.
AC007290 Genomic DNA. Translation: AAD26885.1.
CP002685 Genomic DNA. Translation: AEC07951.1.
AY065067 mRNA. Translation: AAL57700.1.
AY133599 mRNA. Translation: AAM91429.1.
F20043 mRNA. Translation: CAA23388.1.
PIRiH84669.
RefSeqiNP_180287.2. NM_128277.5.
UniGeneiAt.28647.

Genome annotation databases

EnsemblPlantsiAT2G27190.1; AT2G27190.1; AT2G27190.
GeneIDi817261.
KEGGiath:AT2G27190.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF492664 mRNA. Translation: AAM15913.1.
U48448 Genomic DNA. Translation: AAA91803.1.
AC007290 Genomic DNA. Translation: AAD26885.1.
CP002685 Genomic DNA. Translation: AEC07951.1.
AY065067 mRNA. Translation: AAL57700.1.
AY133599 mRNA. Translation: AAM91429.1.
F20043 mRNA. Translation: CAA23388.1.
PIRiH84669.
RefSeqiNP_180287.2. NM_128277.5.
UniGeneiAt.28647.

3D structure databases

ProteinModelPortaliQ38924.
SMRiQ38924. Positions 43-458.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G27190.1.

Proteomic databases

PaxDbiQ38924.
PRIDEiQ38924.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G27190.1; AT2G27190.1; AT2G27190.
GeneIDi817261.
KEGGiath:AT2G27190.

Organism-specific databases

TAIRiAT2G27190.

Phylogenomic databases

eggNOGiCOG1409.
HOGENOMiHOG000238330.
InParanoidiQ38924.
OMAiFMNRYHT.

Miscellaneous databases

PROiQ38924.

Family and domain databases

Gene3Di2.60.40.380. 1 hit.
3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR003961. FN3_dom.
IPR029052. Metallo-depent_PP-like.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
IPR025733. Purple_acid_PPase_C_dom.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
PF14008. Metallophos_C. 1 hit.
[Graphical view]
SMARTiSM00060. FN3. 1 hit.
[Graphical view]
SUPFAMiSSF49363. SSF49363. 1 hit.
SSF56300. SSF56300. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Purple acid phosphatases of Arabidopsis thaliana. Comparative analysis and differential regulation by phosphate deprivation."
    Li D., Zhu H., Liu K., Liu X., Leggewie G., Udvardi M., Wang D.
    J. Biol. Chem. 277:27772-27781(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, GENE FAMILY, NOMENCLATURE.
    Strain: cv. Col-1.
  2. Patel K.S., Lockless S.W., McKnight T.D.
    Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Landsberg erecta.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 138-268.
    Strain: cv. Columbia.
  7. "Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower."
    Zhu H., Qian W., Lu X., Li D., Liu X., Liu K., Wang D.
    Plant Mol. Biol. 59:581-594(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiPPA12_ARATH
AccessioniPrimary (citable) accession number: Q38924
Secondary accession number(s): Q42349
, Q540Q7, Q8VZC6, Q9SHS9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 3, 2011
Last modified: July 22, 2015
This is version 129 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.