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Q38924

- PPA12_ARATH

UniProt

Q38924 - PPA12_ARATH

Protein

Fe(3+)-Zn(2+) purple acid phosphatase 12

Gene

PAP12

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 3 (03 May 2011)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    A phosphate monoester + H2O = an alcohol + phosphate.

    Cofactori

    Binds 1 iron ion per subunit.By similarity
    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi168 – 1681IronBy similarity
    Metal bindingi197 – 1971IronBy similarity
    Metal bindingi197 – 1971ZincBy similarity
    Metal bindingi200 – 2001IronBy similarity
    Metal bindingi234 – 2341ZincBy similarity
    Binding sitei234 – 2341SubstrateBy similarity
    Metal bindingi319 – 3191ZincBy similarity
    Active sitei329 – 3291Proton donorBy similarity
    Metal bindingi356 – 3561ZincBy similarity
    Metal bindingi358 – 3581IronBy similarity

    GO - Molecular functioni

    1. acid phosphatase activity Source: TAIR
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. cellular response to phosphate starvation Source: TAIR
    2. dephosphorylation Source: GOC

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Iron, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fe(3+)-Zn(2+) purple acid phosphatase 12 (EC:3.1.3.2)
    Short name:
    PAP
    Alternative name(s):
    Iron(III)-zinc(II) purple acid phosphatase 12
    Gene namesi
    Name:PAP12
    Synonyms:At10, Ath1, PAP1
    Ordered Locus Names:At2g27190
    ORF Names:T22O13.4
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G27190.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: TAIR
    2. extracellular region Source: UniProtKB-SubCell
    3. plant-type cell wall Source: TAIR

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2828Sequence AnalysisAdd
    BLAST
    Chaini29 – 469441Fe(3+)-Zn(2+) purple acid phosphatase 12PRO_0000023993Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi114 – 1141N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi176 – 1761N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi307 – 3071N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi378 – 378InterchainBy similarity
    Glycosylationi429 – 4291N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ38924.
    PRIDEiQ38924.

    Expressioni

    Tissue specificityi

    Expressed in roots, stems, leaves, flowers and siliques.1 Publication

    Inductioni

    Sligthly by phosphate deprivation.1 Publication

    Gene expression databases

    ArrayExpressiQ38924.
    GenevestigatoriQ38924.

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ38924.
    SMRiQ38924. Positions 43-458.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni356 – 3583Substrate bindingBy similarity

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG1409.
    HOGENOMiHOG000238330.
    InParanoidiQ38924.
    OMAiFVERNLA.

    Family and domain databases

    Gene3Di2.60.40.380. 1 hit.
    3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR003961. Fibronectin_type3.
    IPR029052. Metallo-depent_PP-like.
    IPR008963. Purple_acid_Pase-like_N.
    IPR015914. Purple_acid_Pase_N.
    IPR025733. Purple_acid_PPase_C_dom.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    PF14008. Metallophos_C. 1 hit.
    [Graphical view]
    SMARTiSM00060. FN3. 1 hit.
    [Graphical view]
    SUPFAMiSSF49363. SSF49363. 1 hit.
    SSF56300. SSF56300. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q38924-1 [UniParc]FASTAAdd to Basket

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    MSSRSDLKIK RVSLIIFLLS VLVEFCYGGF TSEYVRGSDL PDDMPLDSDV    50
    FEVPPGPNSP QQVHVTQGNH EGNGVIISWV TPVKPGSKTV QYWCENEKSR 100
    KQAEATVNTY RFFNYTSGYI HHCLIDDLEF DTKYYYEIGS GKWSRRFWFF 150
    IPPKSGPDVP YTFGLIGDLG QTYDSNSTLS HYEMNPGKGQ AVLFVGDLSY 200
    ADRYPNHDNN RWDTWGRFVE RSVAYQPWIW TAGNHEIDFV PDIGEIEPFK 250
    PFMNRYHTPH KASGSISPLW YSIKRASAYI IVMSCYSSYG IYTPQYKWLE 300
    KELQGVNRTE TPWLIVLVHS PFYSSYVHHY MEGETLRVMY EQWFVKYKVD 350
    VVFAGHVHAY ERSERVSNIA YNIVNGLCEP ISDESAPIYI TIGDGGNSEG 400
    LLTDMMQPQP KYSAFREASF GHGLLEIKNR THAYFSWNRN QDGNAVAADS 450
    VWLLNRFWRA QKKTWLDAF 469
    Length:469
    Mass (Da):54,123
    Last modified:May 3, 2011 - v3
    Checksum:i7B75A7A097DAEFAF
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti57 – 571P → H in AAM15913. (PubMed:12021284)Curated
    Sequence conflicti57 – 571P → H in AAD26885. (PubMed:10617197)Curated
    Sequence conflicti91 – 911Q → R in AAA91803. 1 PublicationCurated
    Sequence conflicti97 – 971E → K in AAA91803. 1 PublicationCurated
    Sequence conflicti151 – 1511I → T in AAA91803. 1 PublicationCurated
    Sequence conflicti216 – 2161G → R in CAA23388. (PubMed:8580968)Curated
    Sequence conflicti264 – 2641G → R in CAA23388. (PubMed:8580968)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF492664 mRNA. Translation: AAM15913.1.
    U48448 Genomic DNA. Translation: AAA91803.1.
    AC007290 Genomic DNA. Translation: AAD26885.1.
    CP002685 Genomic DNA. Translation: AEC07951.1.
    AY065067 mRNA. Translation: AAL57700.1.
    AY133599 mRNA. Translation: AAM91429.1.
    F20043 mRNA. Translation: CAA23388.1.
    PIRiH84669.
    RefSeqiNP_180287.2. NM_128277.5.
    UniGeneiAt.28647.

    Genome annotation databases

    EnsemblPlantsiAT2G27190.1; AT2G27190.1; AT2G27190.
    GeneIDi817261.
    KEGGiath:AT2G27190.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF492664 mRNA. Translation: AAM15913.1 .
    U48448 Genomic DNA. Translation: AAA91803.1 .
    AC007290 Genomic DNA. Translation: AAD26885.1 .
    CP002685 Genomic DNA. Translation: AEC07951.1 .
    AY065067 mRNA. Translation: AAL57700.1 .
    AY133599 mRNA. Translation: AAM91429.1 .
    F20043 mRNA. Translation: CAA23388.1 .
    PIRi H84669.
    RefSeqi NP_180287.2. NM_128277.5.
    UniGenei At.28647.

    3D structure databases

    ProteinModelPortali Q38924.
    SMRi Q38924. Positions 43-458.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi Q38924.
    PRIDEi Q38924.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G27190.1 ; AT2G27190.1 ; AT2G27190 .
    GeneIDi 817261.
    KEGGi ath:AT2G27190.

    Organism-specific databases

    TAIRi AT2G27190.

    Phylogenomic databases

    eggNOGi COG1409.
    HOGENOMi HOG000238330.
    InParanoidi Q38924.
    OMAi FVERNLA.

    Gene expression databases

    ArrayExpressi Q38924.
    Genevestigatori Q38924.

    Family and domain databases

    Gene3Di 2.60.40.380. 1 hit.
    3.60.21.10. 1 hit.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR003961. Fibronectin_type3.
    IPR029052. Metallo-depent_PP-like.
    IPR008963. Purple_acid_Pase-like_N.
    IPR015914. Purple_acid_Pase_N.
    IPR025733. Purple_acid_PPase_C_dom.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    PF14008. Metallophos_C. 1 hit.
    [Graphical view ]
    SMARTi SM00060. FN3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49363. SSF49363. 1 hit.
    SSF56300. SSF56300. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Purple acid phosphatases of Arabidopsis thaliana. Comparative analysis and differential regulation by phosphate deprivation."
      Li D., Zhu H., Liu K., Liu X., Leggewie G., Udvardi M., Wang D.
      J. Biol. Chem. 277:27772-27781(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, GENE FAMILY, NOMENCLATURE.
      Strain: cv. Col-1.
    2. Patel K.S., Lockless S.W., McKnight T.D.
      Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Landsberg erecta.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 138-268.
      Strain: cv. Columbia.
    7. "Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower."
      Zhu H., Qian W., Lu X., Li D., Liu X., Liu K., Wang D.
      Plant Mol. Biol. 59:581-594(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiPPA12_ARATH
    AccessioniPrimary (citable) accession number: Q38924
    Secondary accession number(s): Q42349
    , Q540Q7, Q8VZC6, Q9SHS9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: May 3, 2011
    Last modified: October 1, 2014
    This is version 122 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3