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Q38924 (PPA12_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Fe(3+)-Zn(2+) purple acid phosphatase 12
Short name=PAP
EC=3.1.3.2
Alternative name(s):
Iron(III)-zinc(II) purple acid phosphatase 12
Gene names
Name:PAP12
Synonyms:At10, Ath1, PAP1
Ordered Locus Names:At2g27190
ORF Names:T22O13.4
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length469 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactor

Binds 1 iron ion per subunit By similarity.

Binds 1 zinc ion per subunit By similarity.

Subunit structure

Homodimer; disulfide-linked By similarity.

Subcellular location

Secreted.

Tissue specificity

Expressed in roots, stems, leaves, flowers and siliques. Ref.7

Induction

Sligthly by phosphate deprivation. Ref.1

Sequence similarities

Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2828 Potential
Chain29 – 469441Fe(3+)-Zn(2+) purple acid phosphatase 12
PRO_0000023993

Regions

Region356 – 3583Substrate binding By similarity

Sites

Active site3291Proton donor By similarity
Metal binding1681Iron By similarity
Metal binding1971Iron By similarity
Metal binding1971Zinc By similarity
Metal binding2001Iron By similarity
Metal binding2341Zinc By similarity
Metal binding3191Zinc By similarity
Metal binding3561Zinc By similarity
Metal binding3581Iron By similarity
Binding site2341Substrate By similarity

Amino acid modifications

Glycosylation1141N-linked (GlcNAc...) Potential
Glycosylation1761N-linked (GlcNAc...) Potential
Glycosylation3071N-linked (GlcNAc...) Potential
Glycosylation4291N-linked (GlcNAc...) Potential
Disulfide bond378Interchain By similarity

Experimental info

Sequence conflict571P → H in AAM15913. Ref.1
Sequence conflict571P → H in AAD26885. Ref.3
Sequence conflict911Q → R in AAA91803. Ref.2
Sequence conflict971E → K in AAA91803. Ref.2
Sequence conflict1511I → T in AAA91803. Ref.2
Sequence conflict2161G → R in CAA23388. Ref.6
Sequence conflict2641G → R in CAA23388. Ref.6

Sequences

Sequence LengthMass (Da)Tools
Q38924 [UniParc].

Last modified May 3, 2011. Version 3.
Checksum: 7B75A7A097DAEFAF

FASTA46954,123
        10         20         30         40         50         60 
MSSRSDLKIK RVSLIIFLLS VLVEFCYGGF TSEYVRGSDL PDDMPLDSDV FEVPPGPNSP 

        70         80         90        100        110        120 
QQVHVTQGNH EGNGVIISWV TPVKPGSKTV QYWCENEKSR KQAEATVNTY RFFNYTSGYI 

       130        140        150        160        170        180 
HHCLIDDLEF DTKYYYEIGS GKWSRRFWFF IPPKSGPDVP YTFGLIGDLG QTYDSNSTLS 

       190        200        210        220        230        240 
HYEMNPGKGQ AVLFVGDLSY ADRYPNHDNN RWDTWGRFVE RSVAYQPWIW TAGNHEIDFV 

       250        260        270        280        290        300 
PDIGEIEPFK PFMNRYHTPH KASGSISPLW YSIKRASAYI IVMSCYSSYG IYTPQYKWLE 

       310        320        330        340        350        360 
KELQGVNRTE TPWLIVLVHS PFYSSYVHHY MEGETLRVMY EQWFVKYKVD VVFAGHVHAY 

       370        380        390        400        410        420 
ERSERVSNIA YNIVNGLCEP ISDESAPIYI TIGDGGNSEG LLTDMMQPQP KYSAFREASF 

       430        440        450        460 
GHGLLEIKNR THAYFSWNRN QDGNAVAADS VWLLNRFWRA QKKTWLDAF

« Hide

References

« Hide 'large scale' references
[1]"Purple acid phosphatases of Arabidopsis thaliana. Comparative analysis and differential regulation by phosphate deprivation."
Li D., Zhu H., Liu K., Liu X., Leggewie G., Udvardi M., Wang D.
J. Biol. Chem. 277:27772-27781(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, GENE FAMILY, NOMENCLATURE.
Strain: cv. Col-1.
[2]Patel K.S., Lockless S.W., McKnight T.D.
Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Landsberg erecta.
[3]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"Further progress towards a catalogue of all Arabidopsis genes: analysis of a set of 5000 non-redundant ESTs."
Cooke R., Raynal M., Laudie M., Grellet F., Delseny M., Morris P.-C., Guerrier D., Giraudat J., Quigley F., Clabault G., Li Y.-F., Mache R., Krivitzky M., Gy I.J.-J., Kreis M., Lecharny A., Parmentier Y., Marbach J. expand/collapse author list , Fleck J., Clement B., Philipps G., Herve C., Bardet C., Tremousaygue D., Lescure B., Lacomme C., Roby D., Jourjon M.-F., Chabrier P., Charpenteau J.-L., Desprez T., Amselem J., Chiapello H., Hoefte H.
Plant J. 9:101-124(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 138-268.
Strain: cv. Columbia.
[7]"Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower."
Zhu H., Qian W., Lu X., Li D., Liu X., Liu K., Wang D.
Plant Mol. Biol. 59:581-594(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF492664 mRNA. Translation: AAM15913.1.
U48448 Genomic DNA. Translation: AAA91803.1.
AC007290 Genomic DNA. Translation: AAD26885.1.
CP002685 Genomic DNA. Translation: AEC07951.1.
AY065067 mRNA. Translation: AAL57700.1.
AY133599 mRNA. Translation: AAM91429.1.
F20043 mRNA. Translation: CAA23388.1.
IPIIPI00540349.
PIRH84669.
RefSeqNP_180287.2. NM_128277.5.
UniGeneAt.28647.

3D structure databases

ProteinModelPortalQ38924.
SMRQ38924. Positions 43-458.
ModBaseSearch...

Proteomic databases

PaxDbQ38924.
PRIDEQ38924.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G27190.1; AT2G27190.1; AT2G27190.
GeneID817261.
KEGGath:AT2G27190.

Organism-specific databases

TAIRAt2g27190.

Phylogenomic databases

eggNOGCOG1409.
HOGENOMHOG000238330.
InParanoidQ38924.
OMAYSAFREP.
ProtClustDBCLSN2925490.

Gene expression databases

ArrayExpressQ38924.
GenevestigatorQ38924.
GermOnlineAT2G27190. Arabidopsis thaliana.

Family and domain databases

Gene3D2.60.40.380. 1 hit.
InterProIPR003961. Fibronectin_type3.
IPR004843. Metallo_PEstase_dom.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
IPR025733. Purple_acid_PPase_C_dom.
[Graphical view]
PfamPF00149. Metallophos. 1 hit.
PF14008. Metallophos_C. 1 hit.
[Graphical view]
SMARTSM00060. FN3. 1 hit.
[Graphical view]
SUPFAMSSF49363. Purple_Pase_N. 1 hit.
ProtoNetSearch...

Entry information

Entry namePPA12_ARATH
AccessionPrimary (citable) accession number: Q38924
Secondary accession number(s): Q42349 expand/collapse secondary AC list , Q540Q7, Q8VZC6, Q9SHS9
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 3, 2011
Last modified: May 1, 2013
This is version 112 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents