Q38920 (FNTB_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein farnesyltransferase subunit beta
Short name=FTase-beta
EC=2.5.1.58

Alternative name(s):
CAAX farnesyltransferase subunit beta
Enhanced response to abscisic acid 1
Ras proteins prenyltransferase subunit beta

Gene names

Name:	FTB
Synonyms:	ERA1, WIGGUM
Ordered Locus Names:	At5g40280
ORF Names:	MSN9.20

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	482 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the transfer of a farnesyl moiety from farnesyl pyrophosphate to a cysteine at the fourth position from the C-terminus of several proteins. The beta subunit is responsible for peptide-binding By similarity.
Catalytic activity	Farnesyl diphosphate + protein-cysteine = S-farnesyl protein + diphosphate.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Heterodimer of an alpha and a beta subunit By similarity.
Disruption phenotype	Plants show an increase in floral organ number, particularly in the sepals and petals, correlating with an increase in the width of young floral meristems. Ref.1
Sequence similarities	Belongs to the protein prenyltransferase subunit beta family. Contains 5 PFTB repeats.
Sequence caution	The sequence BAB10909.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Prenyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	negative regulation of abscisic acid mediated signaling pathway Inferred from mutant phenotype. Source: TAIR protein prenylation Inferred from mutant phenotype. Source: TAIR regulation of meristem structural organization Inferred from mutant phenotype. Source: TAIR response to abscisic acid stimulus Inferred from mutant phenotype. Source: TAIR response to water deprivation Inferred from mutant phenotype. Source: TAIR
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct protein farnesyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
FTA	Q9LX33	4	EBI-1553332,EBI-1553317

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 482	482	Protein farnesyltransferase subunit beta		PRO_0000119764
Regions
	Repeat	131 – 172	42	PFTB 1
	Repeat	182 – 223	42	PFTB 2
	Repeat	230 – 271	42	PFTB 3
	Repeat	278 – 319	42	PFTB 4
	Repeat	391 – 433	43	PFTB 5
Sites
	Metal binding	304	1	Zinc By similarity
	Metal binding	306	1	Zinc By similarity
	Metal binding	421	1	Zinc By similarity
Experimental info
	Sequence conflict	207	1	M → I in AAA86658. Ref.5
	Sequence conflict	207	1	M → I in AAA87585. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q38920 [UniParc]. Last modified May 31, 2011. Version 3. Checksum: 4A557EB53567C83B	FASTA	482	54,216
	10 20 30 40 50 60 MPVVTRLIRL KCVGLRLDRS GLNRRICHGG HGESTRRRVM EELSSLTVSQ REQFLVENDV 70 80 90 100 110 120 FGIYNYFDAS DVSTQKYMME IQRDKQLDYL MKGLRQLGPQ FSSLDANRPW LCYWILHSIA 130 140 150 160 170 180 LLGETVDDEL ESNAIDFLGR CQGSEGGYGG GPGQLPHLAT TYAAVNALVT LGGDKALSSI 190 200 210 220 230 240 NREKMSCFLR RMKDTSGGFR MHDMGEMDVR ACYTAISVAS ILNIMDDELT QGLGDYILSC 250 260 270 280 290 300 QTYEGGIGGE PGSEAHGGYT YCGLAAMILI NEVDRLNLDS LMNWAVHRQG VEMGFQGRTN 310 320 330 340 350 360 KLVDGCYTFW QAAPCVLLQR LYSTNDHDVH GSSHISEGTN EEHHAHDEDD LEDSDDDDDS 370 380 390 400 410 420 DEDNDEDSVN GHRIHHTSTY INRRMQLVFD SLGLQRYVLL CSKIPDGGFR DKPRKPRDFY 430 440 450 460 470 480 HTCYCLSGLS VAQHAWLKDE DTPPLTRDIM GGYSNLLEPV QLLHNIVMDQ YNEAIEFFFK AA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of the Arabidopsis WIGGUM gene identifies a role for farnesylation in meristem development." Ziegelhoffer E.C., Medrano L.J., Meyerowitz E.M. Proc. Natl. Acad. Sci. U.S.A. 97:7633-7638(2000) [PubMed: 10840062] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], DISRUPTION PHENOTYPE. Strain: cv. Landsberg erecta.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence features of the regions of 1,381,565 bp covered by twenty one physically assigned P1 and TAC clones." Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N., Tabata S. DNA Res. 5:131-145(1998) [PubMed: 9679202] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Arabidopsis ORF clones." Bautista-Mercan V.R., Kim C.J., Chen H., Wu S.Y., De Los Reyes C., Ecker J.R. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	Cutler S.R., Ghassemian M., Bonetta D., Cooney S.E., Mccourt P. Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] OF 79-482.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF214106 mRNA. Translation: AAF74564.1. AB010699 Genomic DNA. Translation: BAB10909.1. Sequence problems. CP002688 Genomic DNA. Translation: AED94529.1. BT030457 mRNA. Translation: ABP88111.1. U46574 Genomic DNA. Translation: AAA87585.1. U44849 mRNA. Translation: AAA86658.1.
IPI	IPI00543310.
RefSeq	NP_198844.1. NM_123392.1.
UniGene	At.9205.
3D structure databases
ProteinModelPortal	Q38920.
SMR	Q38920. Positions 33-481.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q38920. 1 interaction.
STRING	Q38920.
Proteomic databases
PRIDE	Q38920.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G40280.1; AT5G40280.1; AT5G40280.
GeneID	834026.
GenomeReviews	Gene locus AT5G40280 in contig BA000015_GR.
KEGG	ath:AT5G40280.
NMPDR	fig|3702.1.peg.25728.
Organism-specific databases
TAIR	At5g40280.
Phylogenomic databases
InParanoid	Q38920.
ProtClustDB	PLN02710.
Enzyme and pathway databases
BioCyc	ARA:AT5G40280-MONOMER. MetaCyc:AT5G40280-MONOMER.
Gene expression databases
ArrayExpress	Q38920.
Genevestigator	Q38920.
GermOnline	AT5G40280. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR001330. Prenyltrans. IPR008930. Terpenoid_cyclase/PrenylTrfase. [Graphical view]
KO	K05954.
Pfam	PF00432. Prenyltrans. 4 hits. [Graphical view]
SUPFAM	SSF48239. Terp_cyc_toroid. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FNTB_ARATH

Accession

Primary (citable) accession number: Q38920
Secondary accession number(s): A4VCL6, Q38916, Q9LLE9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	May 31, 2011
Last modified:	December 14, 2011
This is version 87 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program