ID ANT_ARATH Reviewed; 555 AA. AC Q38914; Q42462; DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot. DT 22-NOV-2005, sequence version 2. DT 27-MAR-2024, entry version 152. DE RecName: Full=AP2-like ethylene-responsive transcription factor ANT {ECO:0000303|PubMed:8742706, ECO:0000303|PubMed:8742707}; DE AltName: Full=Complementing a protein kinase C mutant protein 1; DE AltName: Full=Protein AINTEGUMENTA {ECO:0000303|PubMed:8742706, ECO:0000303|PubMed:8742707}; DE AltName: Full=Protein DRAGON; DE AltName: Full=Protein OVULE MUTANT {ECO:0000303|PubMed:12271029}; GN Name=ANT {ECO:0000303|PubMed:8742706, ECO:0000303|PubMed:8742707}; GN Synonyms=CKC1, DRG, OVM {ECO:0000303|PubMed:12271029}; GN OrderedLocusNames=At4g37750 {ECO:0000312|Araport:AT4G37750}; GN ORFNames=T28I19.30 {ECO:0000312|EMBL:CAB38923.1}; OS Arabidopsis thaliana (Mouse-ear cress). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis. OX NCBI_TaxID=3702; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND MUTAGENESIS RP OF GLY-382. RC STRAIN=cv. Landsberg erecta; RX PubMed=8742706; DOI=10.1105/tpc.8.2.137; RA Klucher K.M., Chow H., Reiser L., Fischer R.L.; RT "The AINTEGUMENTA gene of Arabidopsis required for ovule and female RT gametophyte development is related to the floral homeotic gene APETALA2."; RL Plant Cell 8:137-153(1996). RN [2] RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL RP STAGE. RC STRAIN=cv. Landsberg erecta; TISSUE=Flower; RX PubMed=8742707; DOI=10.1105/tpc.8.2.155; RA Elliott R.C., Betzner A.S., Huttner E., Oakes M.P., Tucker W.Q.J., RA Gerentes D., Perez P., Smyth D.R.; RT "AINTEGUMENTA, an APETALA2-like gene of Arabidopsis with pleiotropic roles RT in ovule development and floral organ growth."; RL Plant Cell 8:155-168(1996). RN [3] RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION. RX PubMed=9001406; DOI=10.1016/s0014-5793(96)01399-3; RA Vergani P., Morandini P., Soave C.; RT "Complementation of a yeast delta pkc1 mutant by the Arabidopsis protein RT ANT."; RL FEBS Lett. 400:243-246(1997). RN [4] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=cv. Wassilewskija; RA Fischer R.L., Mizukami Y.; RT "Methods for altering mass and fertility in plants."; RL Patent number US6559357, 06-MAY-2003. RN [5] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=cv. Columbia; RX PubMed=10617198; DOI=10.1038/47134; RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M., RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., RA Martienssen R., McCombie W.R.; RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."; RL Nature 402:769-777(1999). RN [6] RP GENOME REANNOTATION. RC STRAIN=cv. Columbia; RX PubMed=27862469; DOI=10.1111/tpj.13415; RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S., RA Town C.D.; RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference RT genome."; RL Plant J. 89:789-804(2017). RN [7] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=cv. Columbia; RX PubMed=14593172; DOI=10.1126/science.1088305; RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L., RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., RA Ecker J.R.; RT "Empirical analysis of transcriptional activity in the Arabidopsis RT genome."; RL Science 302:842-846(2003). RN [8] RP FUNCTION, AND REVIEW. RX PubMed=12271029; DOI=10.1105/tpc.5.10.1291; RA Reiser L., Fischer R.L.; RT "The ovule and the embryo sac."; RL Plant Cell 5:1291-1301(1993). RN [9] RP FUNCTION. RX PubMed=9118807; DOI=10.1242/dev.124.7.1367; RA Schneitz K., Huelskamp M., Kopczak S.D., Pruitt R.E.; RT "Dissection of sexual organ ontogenesis: a genetic analysis of ovule RT development in Arabidopsis thaliana."; RL Development 124:1367-1376(1997). RN [10] RP FUNCTION. RX PubMed=9093862; DOI=10.1093/genetics/145.4.1109; RA Baker S.C., Robinson-Beers K., Villanueva J.M., Gaiser J.C., Gasser C.S.; RT "Interactions among genes regulating ovule development in Arabidopsis RT thaliana."; RL Genetics 145:1109-1124(1997). RN [11] RP DEVELOPMENTAL STAGE. RX PubMed=9671577; DOI=10.1242/dev.125.16.3027; RA Long J.A., Barton M.K.; RT "The development of apical embryonic pattern in Arabidopsis."; RL Development 125:3027-3035(1998). RN [12] RP FUNCTION, AND MUTAGENESIS OF ALA-422. RX PubMed=10528263; RX DOI=10.1002/(sici)1520-6408(1999)25:3<224::aid-dvg5>3.0.co;2-y; RA Krizek B.A.; RT "Ectopic expression of AINTEGUMENTA in Arabidopsis plants results in RT increased growth of floral organs."; RL Dev. Genet. 25:224-236(1999). RN [13] RP DEVELOPMENTAL STAGE. RX PubMed=10656774; DOI=10.1006/dbio.1999.9572; RA Long J., Barton M.K.; RT "Initiation of axillary and floral meristems in Arabidopsis."; RL Dev. Biol. 218:341-353(2000). RN [14] RP DNA-BINDING. RX PubMed=11058102; DOI=10.1093/nar/28.21.4076; RA Nole-Wilson S., Krizek B.A.; RT "DNA binding properties of the Arabidopsis floral development protein RT AINTEGUMENTA."; RL Nucleic Acids Res. 28:4076-4082(2000). RN [15] RP FUNCTION. RX PubMed=10948255; DOI=10.1105/tpc.12.8.1357; RA Krizek B.A., Prost V., Macias A.; RT "AINTEGUMENTA promotes petal identity and acts as a negative regulator of RT AGAMOUS."; RL Plant Cell 12:1357-1366(2000). RN [16] RP FUNCTION. RX PubMed=11041883; DOI=10.1105/tpc.12.10.1879; RA Liu Z., Franks R.G., Klink V.P.; RT "Regulation of gynoecium marginal tissue formation by LEUNIG and RT AINTEGUMENTA."; RL Plant Cell 12:1879-1891(2000). RN [17] RP FUNCTION. RX PubMed=10639184; DOI=10.1073/pnas.97.2.942; RA Mizukami Y., Fischer R.L.; RT "Plant organ size control: AINTEGUMENTA regulates growth and cell numbers RT during organogenesis."; RL Proc. Natl. Acad. Sci. U.S.A. 97:942-947(2000). RN [18] RP FUNCTION. RX PubMed=12183381; DOI=10.1242/dev.129.18.4291; RA Balasubramanian S., Schneitz K.; RT "NOZZLE links proximal-distal and adaxial-abaxial pattern formation during RT ovule development in Arabidopsis thaliana."; RL Development 129:4291-4300(2002). RN [19] RP FUNCTION, AND MUTAGENESIS OF ARG-285; VAL-287; THR-288; HIS-290; TRP-301; RP ASN-303; GLY-314; VAL-317; TYR-318; LEU-319; TYR-322; ASP-333; LEU-337; RP TYR-353; GLU-358; HIS-371; PHE-379; SER-380; GLY-382; SER-384; TYR-386; RP ARG-387; VAL-389; HIS-392; HIS-393; TYR-412; LEU-413; ALA-422; ALA-423; RP ASP-427; ILE-431; ILE-452; 281-THR--ILE-357; 383-ALA--ARG-451 AND RP 452-ILE--SER-555. RX PubMed=12655002; DOI=10.1093/nar/gkg292; RA Krizek B.A.; RT "AINTEGUMENTA utilizes a mode of DNA recognition distinct from that used by RT proteins containing a single AP2 domain."; RL Nucleic Acids Res. 31:1859-1868(2003). RN [20] RP TISSUE SPECIFICITY. RX PubMed=15988559; DOI=10.1007/s11103-005-0955-6; RA Nole-Wilson S., Tranby T.L., Krizek B.A.; RT "AINTEGUMENTA-like (AIL) genes are expressed in young tissues and may RT specify meristematic or division-competent states."; RL Plant Mol. Biol. 57:613-628(2005). RN [21] RP GENE FAMILY, AND NOMENCLATURE. RX PubMed=16407444; DOI=10.1104/pp.105.073783; RA Nakano T., Suzuki K., Fujimura T., Shinshi H.; RT "Genome-wide analysis of the ERF gene family in Arabidopsis and rice."; RL Plant Physiol. 140:411-432(2006). RN [22] RP INTERACTION WITH ANL2; HDG2; HDG10; GL2; HDG3; HDG8; ATML1 AND PDF2. RC STRAIN=cv. Columbia; RX PubMed=25564655; DOI=10.1242/dev.117168; RA Horstman A., Fukuoka H., Muino J.M., Nitsch L., Guo C., Passarinho P., RA Sanchez-Perez G., Immink R., Angenent G., Boutilier K.; RT "AIL and HDG proteins act antagonistically to control cell proliferation."; RL Development 142:454-464(2015). CC -!- FUNCTION: Transcription activator that recognizes and binds to the DNA CC consensus sequence 5'-CAC[AG]N[AT]TNCCNANG-3'. Required for the CC initiation and growth of ovules integumenta, and for the development of CC female gametophyte. Plays a critical role in the development of CC gynoecium marginal tissues (e.g. stigma, style and septa), and in the CC fusion of carpels and of medial ridges leading to ovule primordia. Also CC involved in organs initiation and development, including floral organs. CC Maintains the meristematic competence of cells and consequently CC sustains expression of cell cycle regulators during organogenesis, thus CC controlling the final size of each organ by controlling their cell CC number. Regulates INO autoinduction and expression pattern. As ANT CC promotes petal cell identity and mediates down-regulation of AG in CC flower whorl 2, it functions as a class A homeotic gene. CC {ECO:0000269|PubMed:10528263, ECO:0000269|PubMed:10639184, CC ECO:0000269|PubMed:10948255, ECO:0000269|PubMed:11041883, CC ECO:0000269|PubMed:12183381, ECO:0000269|PubMed:12271029, CC ECO:0000269|PubMed:12655002, ECO:0000269|PubMed:8742706, CC ECO:0000269|PubMed:8742707, ECO:0000269|PubMed:9001406, CC ECO:0000269|PubMed:9093862, ECO:0000269|PubMed:9118807}. CC -!- SUBUNIT: Interacts with ANL2, HDG2 and HDG10, and possibly with GL2, CC HDG3, HDG8, ATML1 and PDF2. {ECO:0000269|PubMed:25564655}. CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}. CC -!- TISSUE SPECIFICITY: Mostly expressed in developing flowers. Also CC present in mature flowers, siliques and seedlings, but not in mature CC roots, leaves and stems. Expressed in ovules and in vegetative and CC floral primordia. {ECO:0000269|PubMed:15988559, CC ECO:0000269|PubMed:8742706, ECO:0000269|PubMed:8742707}. CC -!- DEVELOPMENTAL STAGE: Expressed in floral primordia, in STM-negative CC region, then in sepal primordia. As sepal develops, progressively CC confined to a basal core before disappearing. Present in stamen CC primordia, then confined to a central region as they become stalked and CC develop locules. Later reduced to procambial cells as stamen mature. CC From petal primordia, expressed on the lateral edges of developing CC petals and finally confined to petal epidermis before disappearing. CC Present in carpel primordia, then in inner side of carpels especially CC in the placenta. Strong levels in ovules primordia and young ovules, CC then localized in integuments initiation zone before being confined to CC inner integument cells that will differentiate into the endothelium. CC Expressed in the distal half of the funiculus throughout ovule CC development and later extends into the chalaza. After fertilization, CC expression shift to the embryo. First on the apical part at the CC globular stage, then in cotyledons primordia, and later in cotyledons CC during the torpedo stage. As cotyledons grow out, expression becomes CC limited to a plane separating adaxial and abaxial parts. Excluded from CC the embryonic central region (ECR). In seedlings, found in leaf CC primordia then in central and lateral actively developing regions of CC extending leaves. {ECO:0000269|PubMed:10656774, CC ECO:0000269|PubMed:8742707, ECO:0000269|PubMed:9671577}. CC -!- SIMILARITY: Belongs to the AP2/ERF transcription factor family. AP2 CC subfamily. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; U40256; AAA91040.1; -; mRNA. DR EMBL; U41339; AAB17364.1; -; mRNA. DR EMBL; U44028; AAA86281.1; -; mRNA. DR EMBL; AR316367; -; NOT_ANNOTATED_CDS; Genomic_DNA. DR EMBL; AL035709; CAB38923.1; -; Genomic_DNA. DR EMBL; AL161592; CAB80440.1; -; Genomic_DNA. DR EMBL; CP002687; AEE86834.1; -; Genomic_DNA. DR EMBL; AY080706; AAL85024.1; -; mRNA. DR EMBL; AY117207; AAM51282.1; -; mRNA. DR PIR; S71365; S71365. DR RefSeq; NP_195489.1; NM_119937.3. DR AlphaFoldDB; Q38914; -. DR SMR; Q38914; -. DR IntAct; Q38914; 4. DR STRING; 3702.Q38914; -. DR PaxDb; 3702-AT4G37750-1; -. DR ProteomicsDB; 240880; -. DR EnsemblPlants; AT4G37750.1; AT4G37750.1; AT4G37750. DR GeneID; 829931; -. DR Gramene; AT4G37750.1; AT4G37750.1; AT4G37750. DR KEGG; ath:AT4G37750; -. DR Araport; AT4G37750; -. DR TAIR; AT4G37750; ANT. DR eggNOG; ENOG502QQ82; Eukaryota. DR HOGENOM; CLU_013549_4_0_1; -. DR InParanoid; Q38914; -. DR OMA; MPHMPVF; -. DR OrthoDB; 397053at2759; -. DR PhylomeDB; Q38914; -. DR PRO; PR:Q38914; -. DR Proteomes; UP000006548; Chromosome 4. DR ExpressionAtlas; Q38914; baseline and differential. DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell. DR GO; GO:0003677; F:DNA binding; IDA:TAIR. DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR. DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR. DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW. DR GO; GO:0051301; P:cell division; IMP:TAIR. DR GO; GO:0009908; P:flower development; IEA:UniProtKB-KW. DR GO; GO:0019760; P:glucosinolate metabolic process; IMP:TAIR. DR GO; GO:0010492; P:maintenance of shoot apical meristem identity; IGI:TAIR. DR GO; GO:0010601; P:positive regulation of auxin biosynthetic process; IPI:TAIR. DR GO; GO:0042127; P:regulation of cell population proliferation; IMP:TAIR. DR CDD; cd00018; AP2; 2. DR Gene3D; 3.30.730.10; AP2/ERF domain; 2. DR InterPro; IPR001471; AP2/ERF_dom. DR InterPro; IPR036955; AP2/ERF_dom_sf. DR InterPro; IPR016177; DNA-bd_dom_sf. DR PANTHER; PTHR32467; AP2-LIKE ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR; 1. DR PANTHER; PTHR32467:SF264; AP2-LIKE ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR ANT; 1. DR Pfam; PF00847; AP2; 2. DR PRINTS; PR00367; ETHRSPELEMNT. DR SMART; SM00380; AP2; 2. DR SUPFAM; SSF54171; DNA-binding domain; 2. DR PROSITE; PS51032; AP2_ERF; 2. DR Genevisible; Q38914; AT. PE 1: Evidence at protein level; KW Activator; Developmental protein; Differentiation; DNA-binding; Flowering; KW Nucleus; Reference proteome; Repeat; Transcription; KW Transcription regulation. FT CHAIN 1..555 FT /note="AP2-like ethylene-responsive transcription factor FT ANT" FT /id="PRO_0000112524" FT DNA_BIND 283..349 FT /note="AP2/ERF 1" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00366" FT DNA_BIND 385..443 FT /note="AP2/ERF 2" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00366" FT REGION 34..56 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 199..231 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 38..56 FT /note="Polar residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT MUTAGEN 281..357 FT /note="Missing: Loss of activation of transcription." FT /evidence="ECO:0000269|PubMed:12655002" FT MUTAGEN 285 FT /note="R->G: Reduced activation of transcription." FT /evidence="ECO:0000269|PubMed:12655002" FT MUTAGEN 287 FT /note="V->A: Loss of activation of transcription; when FT associated with D-303." FT /evidence="ECO:0000269|PubMed:12655002" FT MUTAGEN 287 FT /note="V->D: Loss of activation of transcription." FT /evidence="ECO:0000269|PubMed:12655002" FT MUTAGEN 288 FT /note="T->A: Reduced activation of transcription." FT /evidence="ECO:0000269|PubMed:12655002" FT MUTAGEN 290 FT /note="H->A: Reduced activation of transcription." FT /evidence="ECO:0000269|PubMed:12655002" FT MUTAGEN 301 FT /note="W->R: Reduced DNA-binding and loss of activation of FT transcription." FT /evidence="ECO:0000269|PubMed:12655002" FT MUTAGEN 303 FT /note="N->D: Loss of activation of transcription; when FT associated with A-287." FT /evidence="ECO:0000269|PubMed:12655002" FT MUTAGEN 314 FT /note="G->R: Loss of activation of transcription; when FT associated with C-353." FT /evidence="ECO:0000269|PubMed:12655002" FT MUTAGEN 317 FT /note="V->D: No effect on DNA-binding but reduced FT activation of transcription." FT /evidence="ECO:0000269|PubMed:12655002" FT MUTAGEN 318 FT /note="Y->C: Reduced DNA-binding and loss of activation of FT transcription." FT /evidence="ECO:0000269|PubMed:12655002" FT MUTAGEN 319 FT /note="L->P: Loss of activation of transcription." FT /evidence="ECO:0000269|PubMed:12655002" FT MUTAGEN 319 FT /note="L->Q: Reduced DNA-binding and loss of activation of FT transcription." FT /evidence="ECO:0000269|PubMed:12655002" FT MUTAGEN 322 FT /note="Y->F: Loss of activation of transcription; when FT associated with D-386." FT /evidence="ECO:0000269|PubMed:12655002" FT MUTAGEN 333 FT /note="D->E: Loss of activation of transcription; when FT associated with T-452." FT /evidence="ECO:0000269|PubMed:12655002" FT MUTAGEN 333 FT /note="D->G: Reduced DNA-binding and loss of activation of FT transcription." FT /evidence="ECO:0000269|PubMed:12655002" FT MUTAGEN 337 FT /note="L->P: Reduced DNA-binding and loss of activation of FT transcription." FT /evidence="ECO:0000269|PubMed:12655002" FT MUTAGEN 353 FT /note="Y->C: Loss of activation of transcription; when FT associated with R-314." FT /evidence="ECO:0000269|PubMed:12655002" FT MUTAGEN 358 FT /note="E->G: Loss of activation of transcription." FT /evidence="ECO:0000269|PubMed:12655002" FT MUTAGEN 371 FT /note="H->P: Loss of activation of transcription; when FT associated with A-389." FT /evidence="ECO:0000269|PubMed:12655002" FT MUTAGEN 379 FT /note="F->S: Loss of activation of transcription." FT /evidence="ECO:0000269|PubMed:12655002" FT MUTAGEN 380 FT /note="S->P: Reduced DNA-binding and reduced activation of FT transcription." FT /evidence="ECO:0000269|PubMed:12655002" FT MUTAGEN 382 FT /note="G->D: In ant-2; female-sterile, loss of ovule FT integuments initiation, impaired female gametophyte FT development, abnormal floral organs, and loss of activation FT of transcription." FT /evidence="ECO:0000269|PubMed:12655002, FT ECO:0000269|PubMed:8742706" FT MUTAGEN 383..451 FT /note="Missing: Loss of activation of transcription." FT /evidence="ECO:0000269|PubMed:12655002" FT MUTAGEN 384 FT /note="S->P: Loss of activation of transcription." FT /evidence="ECO:0000269|PubMed:12655002" FT MUTAGEN 386 FT /note="Y->D: Loss of activation of transcription; when FT associated with F-322." FT /evidence="ECO:0000269|PubMed:12655002" FT MUTAGEN 387 FT /note="R->G: Reduced DNA-binding and loss of activation of FT transcription." FT /evidence="ECO:0000269|PubMed:12655002" FT MUTAGEN 389 FT /note="V->A: Loss of activation of transcription; when FT associated with P-371." FT /evidence="ECO:0000269|PubMed:12655002" FT MUTAGEN 389 FT /note="V->D: Reduced activation of transcription." FT /evidence="ECO:0000269|PubMed:12655002" FT MUTAGEN 392 FT /note="H->A: Reduced activation of transcription." FT /evidence="ECO:0000269|PubMed:12655002" FT MUTAGEN 393 FT /note="H->A: Reduced activation of transcription." FT /evidence="ECO:0000269|PubMed:12655002" FT MUTAGEN 412 FT /note="Y->C: Reduced activation of transcription." FT /evidence="ECO:0000269|PubMed:12655002" FT MUTAGEN 413 FT /note="L->Q: Reduced activation of transcription." FT /evidence="ECO:0000269|PubMed:12655002" FT MUTAGEN 422 FT /note="A->T: In ant-8; abnormal floral organs and ovule FT development." FT /evidence="ECO:0000269|PubMed:10528263, FT ECO:0000269|PubMed:12655002" FT MUTAGEN 423 FT /note="A->T: Reduced DNA-binding and loss of activation of FT transcription." FT /evidence="ECO:0000269|PubMed:12655002" FT MUTAGEN 427 FT /note="D->G: Reduced DNA-binding and loss of activation of FT transcription." FT /evidence="ECO:0000269|PubMed:12655002" FT MUTAGEN 431 FT /note="I->S: Reduced DNA-binding and reduced activation of FT transcription." FT /evidence="ECO:0000269|PubMed:12655002" FT MUTAGEN 452..555 FT /note="Missing: No effect on activation of transcription." FT /evidence="ECO:0000269|PubMed:12655002" FT MUTAGEN 452 FT /note="I->T: Loss of activation of transcription; when FT associated with E-333." FT /evidence="ECO:0000269|PubMed:12655002" FT CONFLICT 45 FT /note="S -> C (in Ref. 3; AAA86281)" FT /evidence="ECO:0000305" SQ SEQUENCE 555 AA; 61725 MW; BD275D2F082B519B CRC64; MKSFCDNDDN NHSNTTNLLG FSLSSNMMKM GGRGGREAIY SSSTSSAATS SSSVPPQLVV GDNTSNFGVC YGSNPNGGIY SHMSVMPLRS DGSLCLMEAL NRSSHSNHHQ DSSPKVEDFF GTHHNNTSHK EAMDLSLDSL FYNTTHEPNT TTNFQEFFSF PQTRNHEEET RNYGNDPSLT HGGSFNVGVY GEFQQSLSLS MSPGSQSSCI TGSHHHQQNQ NQNHQSQNHQ QISEALVETS VGFETTTMAA AKKKRGQEDV VVVGQKQIVH RKSIDTFGQR TSQYRGVTRH RWTGRYEAHL WDNSFKKEGH SRKGRQVYLG GYDMEEKAAR AYDLAALKYW GPSTHTNFSA ENYQKEIEDM KNMTRQEYVA HLRRKSSGFS RGASIYRGVT RHHQHGRWQA RIGRVAGNKD LYLGTFGTQE EAAEAYDVAA IKFRGTNAVT NFDITRYDVD RIMSSNTLLS GELARRNNNS IVVRNTEDQT ALNAVVEGGS NKEVSTPERL LSFPAIFALP QVNQKMFGSN MGGNMSPWTS NPNAELKTVA LTLPQMPVFA AWADS //