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Protein

AP2-like ethylene-responsive transcription factor ANT

Gene

ANT

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription activator that recognizes and binds to the DNA consensus sequence 5'-CAC[AG]N[AT]TNCCNANG-3'. Required for the initiation and growth of ovules integumenta, and for the development of female gametophyte. Plays a critical role in the development of gynoecium marginal tissues (e.g. stigma, style and septa), and in the fusion of carpels and of medial ridges leading to ovule primordia. Also involved in organs initiation and development, including floral organs. Maintains the meristematic competence of cells and consequently sustains expression of cell cycle regulators during organogenesis, thus controlling the final size of each organ by controlling their cell number. Regulates INO autoinduction and expression pattern. As ANT promotes petal cell identity and mediates down-regulation of AG in flower whorl 2, it functions as a class A homeotic gene.12 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi283 – 349AP2/ERF 1PROSITE-ProRule annotationAdd BLAST67
DNA bindingi385 – 443AP2/ERF 2PROSITE-ProRule annotationAdd BLAST59

GO - Molecular functioni

  • DNA binding Source: TAIR
  • sequence-specific DNA binding Source: TAIR
  • transcription factor activity, sequence-specific DNA binding Source: TAIR

GO - Biological processi

  • cell differentiation Source: UniProtKB-KW
  • flower development Source: UniProtKB-KW
  • glucosinolate metabolic process Source: TAIR
  • maintenance of shoot apical meristem identity Source: TAIR
  • regulation of cell proliferation Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Differentiation, Flowering, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
AP2-like ethylene-responsive transcription factor ANT
Alternative name(s):
Complementing a protein kinase C mutant protein 1
Protein AINTEGUMENTA
Protein DRAGON
Protein OVULE MUTANT
Gene namesi
Name:ANT
Synonyms:CKC1, DRG, OVM
Ordered Locus Names:At4g37750
ORF Names:T28I19.30
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G37750.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi281 – 357Missing : Loss of activation of transcription. 1 PublicationAdd BLAST77
Mutagenesisi285R → G: Reduced activation of transcription. 1 Publication1
Mutagenesisi287V → A: Loss of activation of transcription; when associated with D-303. 1 Publication1
Mutagenesisi287V → D: Loss of activation of transcription. 1 Publication1
Mutagenesisi288T → A: Reduced activation of transcription. 1 Publication1
Mutagenesisi290H → A: Reduced activation of transcription. 1 Publication1
Mutagenesisi301W → R: Reduced DNA-binding and loss of activation of transcription. 1 Publication1
Mutagenesisi303N → D: Loss of activation of transcription; when associated with A-287. 1 Publication1
Mutagenesisi314G → R: Loss of activation of transcription; when associated with C-353. 1 Publication1
Mutagenesisi317V → D: No effect on DNA-binding but reduced activation of transcription. 1 Publication1
Mutagenesisi318Y → C: Reduced DNA-binding and loss of activation of transcription. 1 Publication1
Mutagenesisi319L → P: Loss of activation of transcription. 1 Publication1
Mutagenesisi319L → Q: Reduced DNA-binding and loss of activation of transcription. 1 Publication1
Mutagenesisi322Y → F: Loss of activation of transcription; when associated with D-386. 1 Publication1
Mutagenesisi333D → E: Loss of activation of transcription; when associated with T-452. 1 Publication1
Mutagenesisi333D → G: Reduced DNA-binding and loss of activation of transcription. 1 Publication1
Mutagenesisi337L → P: Reduced DNA-binding and loss of activation of transcription. 1 Publication1
Mutagenesisi353Y → C: Loss of activation of transcription; when associated with R-314. 1 Publication1
Mutagenesisi358E → G: Loss of activation of transcription. 1 Publication1
Mutagenesisi371H → P: Loss of activation of transcription; when associated with A-389. 1 Publication1
Mutagenesisi379F → S: Loss of activation of transcription. 1 Publication1
Mutagenesisi380S → P: Reduced DNA-binding and reduced activation of transcription. 1 Publication1
Mutagenesisi382G → D in ant-2; female-sterile, loss of ovule integuments initiation, impaired female gametophyte development, abnormal floral organs, and loss of activation of transcription. 2 Publications1
Mutagenesisi383 – 451Missing : Loss of activation of transcription. 1 PublicationAdd BLAST69
Mutagenesisi384S → P: Loss of activation of transcription. 1 Publication1
Mutagenesisi386Y → D: Loss of activation of transcription; when associated with F-322. 1 Publication1
Mutagenesisi387R → G: Reduced DNA-binding and loss of activation of transcription. 1 Publication1
Mutagenesisi389V → A: Loss of activation of transcription; when associated with P-371. 1 Publication1
Mutagenesisi389V → D: Reduced activation of transcription. 1 Publication1
Mutagenesisi392H → A: Reduced activation of transcription. 1 Publication1
Mutagenesisi393H → A: Reduced activation of transcription. 1 Publication1
Mutagenesisi412Y → C: Reduced activation of transcription. 1 Publication1
Mutagenesisi413L → Q: Reduced activation of transcription. 1 Publication1
Mutagenesisi422A → T in ant-8; abnormal floral organs and ovule development. 2 Publications1
Mutagenesisi423A → T: Reduced DNA-binding and loss of activation of transcription. 1 Publication1
Mutagenesisi427D → G: Reduced DNA-binding and loss of activation of transcription. 1 Publication1
Mutagenesisi431I → S: Reduced DNA-binding and reduced activation of transcription. 1 Publication1
Mutagenesisi452 – 555Missing : No effect on activation of transcription. 1 PublicationAdd BLAST104
Mutagenesisi452I → T: Loss of activation of transcription; when associated with E-333. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001125241 – 555AP2-like ethylene-responsive transcription factor ANTAdd BLAST555

Proteomic databases

PaxDbiQ38914.

Expressioni

Tissue specificityi

Mostly expressed in developing flowers. Also present in mature flowers, siliques and seedlings, but not in mature roots, leaves and stems. Expressed in ovules and in vegetative and floral primordia.3 Publications

Developmental stagei

Expressed in floral primordia, in STM-negative region, then in sepal primordia. As sepal develops, progressively confined to a basal core before disappearing. Present in stamen primordia, then confined to a central region as they become stalked and develop locules. Later reduced to procambial cells as stamen mature. From petal primordia, expressed on the lateral edges of developing petals and finally confined to petal epidermis before disappearing. Present in carpel primordia, then in inner side of carpels especially in the placenta. Strong levels in ovules primordia and young ovules, then localized in integuments initiation zone before being confined to inner integument cells that will differentiate into the endothelium. Expressed in the distal half of the funiculus throughout ovule development and later extends into the chalaza. After fertilization, expression shift to the embryo. First on the apical part at the globular stage, then in cotyledons primordia, and later in cotyledons during the torpedo stage. As cotyledons grow out, expression becomes limited to a plane separating adaxial and abaxial parts. Excluded from the embryonic central region (ECR). In seedlings, found in leaf primordia then in central and lateral actively developing regions of extending leaves.3 Publications

Gene expression databases

GenevisibleiQ38914. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G37750.1.

Structurei

3D structure databases

ProteinModelPortaliQ38914.
SMRiQ38914.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi41 – 53Ser-richAdd BLAST13
Compositional biasi217 – 231Gln-richAdd BLAST15

Sequence similaritiesi

Contains 2 AP2/ERF DNA-binding domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IEVJ. Eukaryota.
ENOG410Y9X6. LUCA.
HOGENOMiHOG000239258.
InParanoidiQ38914.
KOiK09285.
OMAiTSHKEAM.
OrthoDBiEOG09360BG2.
PhylomeDBiQ38914.

Family and domain databases

CDDicd00018. AP2. 2 hits.
Gene3Di3.30.730.10. 2 hits.
InterProiIPR031112. AP2-like_transcript_factor.
IPR001471. AP2/ERF_dom.
IPR016177. DNA-bd_dom.
[Graphical view]
PANTHERiPTHR32467. PTHR32467. 1 hit.
PfamiPF00847. AP2. 2 hits.
[Graphical view]
PRINTSiPR00367. ETHRSPELEMNT.
SMARTiSM00380. AP2. 2 hits.
[Graphical view]
SUPFAMiSSF54171. SSF54171. 2 hits.
PROSITEiPS51032. AP2_ERF. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q38914-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSFCDNDDN NHSNTTNLLG FSLSSNMMKM GGRGGREAIY SSSTSSAATS
60 70 80 90 100
SSSVPPQLVV GDNTSNFGVC YGSNPNGGIY SHMSVMPLRS DGSLCLMEAL
110 120 130 140 150
NRSSHSNHHQ DSSPKVEDFF GTHHNNTSHK EAMDLSLDSL FYNTTHEPNT
160 170 180 190 200
TTNFQEFFSF PQTRNHEEET RNYGNDPSLT HGGSFNVGVY GEFQQSLSLS
210 220 230 240 250
MSPGSQSSCI TGSHHHQQNQ NQNHQSQNHQ QISEALVETS VGFETTTMAA
260 270 280 290 300
AKKKRGQEDV VVVGQKQIVH RKSIDTFGQR TSQYRGVTRH RWTGRYEAHL
310 320 330 340 350
WDNSFKKEGH SRKGRQVYLG GYDMEEKAAR AYDLAALKYW GPSTHTNFSA
360 370 380 390 400
ENYQKEIEDM KNMTRQEYVA HLRRKSSGFS RGASIYRGVT RHHQHGRWQA
410 420 430 440 450
RIGRVAGNKD LYLGTFGTQE EAAEAYDVAA IKFRGTNAVT NFDITRYDVD
460 470 480 490 500
RIMSSNTLLS GELARRNNNS IVVRNTEDQT ALNAVVEGGS NKEVSTPERL
510 520 530 540 550
LSFPAIFALP QVNQKMFGSN MGGNMSPWTS NPNAELKTVA LTLPQMPVFA

AWADS
Length:555
Mass (Da):61,725
Last modified:November 22, 2005 - v2
Checksum:iBD275D2F082B519B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti45S → C in AAA86281 (PubMed:9001406).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40256 mRNA. Translation: AAA91040.1.
U41339 mRNA. Translation: AAB17364.1.
U44028 mRNA. Translation: AAA86281.1.
AR316367 Genomic DNA. No translation available.
AL035709 Genomic DNA. Translation: CAB38923.1.
AL161592 Genomic DNA. Translation: CAB80440.1.
CP002687 Genomic DNA. Translation: AEE86834.1.
AY080706 mRNA. Translation: AAL85024.1.
AY117207 mRNA. Translation: AAM51282.1.
PIRiS71365.
RefSeqiNP_195489.1. NM_119937.3.
UniGeneiAt.1556.

Genome annotation databases

EnsemblPlantsiAT4G37750.1; AT4G37750.1; AT4G37750.
GeneIDi829931.
GrameneiAT4G37750.1; AT4G37750.1; AT4G37750.
KEGGiath:AT4G37750.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40256 mRNA. Translation: AAA91040.1.
U41339 mRNA. Translation: AAB17364.1.
U44028 mRNA. Translation: AAA86281.1.
AR316367 Genomic DNA. No translation available.
AL035709 Genomic DNA. Translation: CAB38923.1.
AL161592 Genomic DNA. Translation: CAB80440.1.
CP002687 Genomic DNA. Translation: AEE86834.1.
AY080706 mRNA. Translation: AAL85024.1.
AY117207 mRNA. Translation: AAM51282.1.
PIRiS71365.
RefSeqiNP_195489.1. NM_119937.3.
UniGeneiAt.1556.

3D structure databases

ProteinModelPortaliQ38914.
SMRiQ38914.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT4G37750.1.

Proteomic databases

PaxDbiQ38914.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G37750.1; AT4G37750.1; AT4G37750.
GeneIDi829931.
GrameneiAT4G37750.1; AT4G37750.1; AT4G37750.
KEGGiath:AT4G37750.

Organism-specific databases

TAIRiAT4G37750.

Phylogenomic databases

eggNOGiENOG410IEVJ. Eukaryota.
ENOG410Y9X6. LUCA.
HOGENOMiHOG000239258.
InParanoidiQ38914.
KOiK09285.
OMAiTSHKEAM.
OrthoDBiEOG09360BG2.
PhylomeDBiQ38914.

Miscellaneous databases

PROiQ38914.

Gene expression databases

GenevisibleiQ38914. AT.

Family and domain databases

CDDicd00018. AP2. 2 hits.
Gene3Di3.30.730.10. 2 hits.
InterProiIPR031112. AP2-like_transcript_factor.
IPR001471. AP2/ERF_dom.
IPR016177. DNA-bd_dom.
[Graphical view]
PANTHERiPTHR32467. PTHR32467. 1 hit.
PfamiPF00847. AP2. 2 hits.
[Graphical view]
PRINTSiPR00367. ETHRSPELEMNT.
SMARTiSM00380. AP2. 2 hits.
[Graphical view]
SUPFAMiSSF54171. SSF54171. 2 hits.
PROSITEiPS51032. AP2_ERF. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiANT_ARATH
AccessioniPrimary (citable) accession number: Q38914
Secondary accession number(s): Q42462
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 22, 2005
Last modified: November 30, 2016
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.