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Q38909

- XTH28_ARATH

UniProt

Q38909 - XTH28_ARATH

Protein

Probable xyloglucan endotransglucosylase/hydrolase protein 28

Gene

XTH28

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues By similarity.By similarity

    Catalytic activityi

    Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei108 – 1081NucleophileBy similarity
    Active sitei112 – 1121Proton donorBy similarity

    GO - Molecular functioni

    1. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro
    2. xyloglucan:xyloglucosyl transferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellular glucan metabolic process Source: InterPro
    2. fruit development Source: TAIR
    3. stamen filament development Source: TAIR

    Keywords - Molecular functioni

    Glycosidase, Hydrolase, Transferase

    Keywords - Biological processi

    Cell wall biogenesis/degradation

    Enzyme and pathway databases

    BioCyciARA:AT1G14720-MONOMER.

    Protein family/group databases

    CAZyiGH16. Glycoside Hydrolase Family 16.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable xyloglucan endotransglucosylase/hydrolase protein 28 (EC:2.4.1.207)
    Short name:
    At-XTH28
    Short name:
    XTH-28
    Gene namesi
    Name:XTH28
    Synonyms:EXGT-A2, XTR2
    Ordered Locus Names:At1g14720
    ORF Names:F10B6.12, F10B6.29
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G14720.

    Subcellular locationi

    GO - Cellular componenti

    1. apoplast Source: UniProtKB-SubCell
    2. cell wall Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Apoplast, Cell wall, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222Sequence AnalysisAdd
    BLAST
    Chaini23 – 332310Probable xyloglucan endotransglucosylase/hydrolase protein 28PRO_0000011828Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi131 – 1311N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Contains at least one intrachain disulfide bond essential for its enzymatic activity.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiQ38909.

    Expressioni

    Tissue specificityi

    Expressed in 7 day old seedlings, roots, rosette leaves, internodes between nodes bearing axillary shoots, nodes bearing flowers, flower buds and siliques.1 Publication

    Gene expression databases

    ArrayExpressiQ38909.
    GenevestigatoriQ38909.

    Interactioni

    Protein-protein interaction databases

    MINTiMINT-8067472.
    STRINGi3702.AT1G14720.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ38909.
    SMRiQ38909. Positions 31-290.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG2273.
    HOGENOMiHOG000236368.
    InParanoidiQ38909.
    KOiK08235.
    OMAiSHIIFYV.
    PhylomeDBiQ38909.

    Family and domain databases

    Gene3Di2.60.120.200. 1 hit.
    InterProiIPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    IPR016455. XET.
    IPR010713. XET_C.
    [Graphical view]
    PfamiPF00722. Glyco_hydro_16. 1 hit.
    PF06955. XET_C. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005604. XET. 1 hit.
    SUPFAMiSSF49899. SSF49899. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q38909-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGFITRFLVF MSLFTSLVSG FALQKLPLIQ FDEGYTQLFG DQNLIVHRDG    50
    KSVRLTLDER TGSGFVSNDI YLHGFFSSSI KLPADYSAGV VIAFYLSNGD 100
    LYEKNHDEID FEFLGNIRGR EWRIQTNIYG NGSTHLGREE RYNLWFDPTE 150
    DFHQYSILWS LSHIIFYVDN VPIREVKRTA SMGGDFPAKP MSLYSTIWDG 200
    SKWATDGGKY GVNYKYAPYV SQFTDLILHG CAVDPTEKFP SCKDEAVQNL 250
    RLASEITESQ RNKMEIFRQK HMTYSYCYDH MRYKVVLSEC VVNPAEAKRL 300
    RVYDPVTFGG IPHGHRRGKH RSRSRLARTE SI 332
    Length:332
    Mass (Da):38,252
    Last modified:November 1, 1996 - v1
    Checksum:iAF8242902A0CA191
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U43487 mRNA. Translation: AAB18366.1.
    AF163820 Genomic DNA. Translation: AAD45124.1.
    D63510 mRNA. Translation: BAA20290.1.
    AC006917 Genomic DNA. Translation: AAF79246.1.
    CP002684 Genomic DNA. Translation: AEE29214.1.
    AF385714 mRNA. Translation: AAK60305.1.
    AY085855 mRNA. Translation: AAM63068.1.
    PIRiS71224.
    RefSeqiNP_172925.1. NM_101341.3.
    UniGeneiAt.279.

    Genome annotation databases

    EnsemblPlantsiAT1G14720.1; AT1G14720.1; AT1G14720.
    GeneIDi838037.
    KEGGiath:AT1G14720.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U43487 mRNA. Translation: AAB18366.1 .
    AF163820 Genomic DNA. Translation: AAD45124.1 .
    D63510 mRNA. Translation: BAA20290.1 .
    AC006917 Genomic DNA. Translation: AAF79246.1 .
    CP002684 Genomic DNA. Translation: AEE29214.1 .
    AF385714 mRNA. Translation: AAK60305.1 .
    AY085855 mRNA. Translation: AAM63068.1 .
    PIRi S71224.
    RefSeqi NP_172925.1. NM_101341.3.
    UniGenei At.279.

    3D structure databases

    ProteinModelPortali Q38909.
    SMRi Q38909. Positions 31-290.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-8067472.
    STRINGi 3702.AT1G14720.1-P.

    Protein family/group databases

    CAZyi GH16. Glycoside Hydrolase Family 16.

    Proteomic databases

    PRIDEi Q38909.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G14720.1 ; AT1G14720.1 ; AT1G14720 .
    GeneIDi 838037.
    KEGGi ath:AT1G14720.

    Organism-specific databases

    GeneFarmi 2644. 265.
    TAIRi AT1G14720.

    Phylogenomic databases

    eggNOGi COG2273.
    HOGENOMi HOG000236368.
    InParanoidi Q38909.
    KOi K08235.
    OMAi SHIIFYV.
    PhylomeDBi Q38909.

    Enzyme and pathway databases

    BioCyci ARA:AT1G14720-MONOMER.

    Gene expression databases

    ArrayExpressi Q38909.
    Genevestigatori Q38909.

    Family and domain databases

    Gene3Di 2.60.120.200. 1 hit.
    InterProi IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    IPR016455. XET.
    IPR010713. XET_C.
    [Graphical view ]
    Pfami PF00722. Glyco_hydro_16. 1 hit.
    PF06955. XET_C. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005604. XET. 1 hit.
    SUPFAMi SSF49899. SSF49899. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The Arabidopsis XET-related gene family: environmental and hormonal regulation of expression."
      Xu W., Campbell P., Vargheese A.K., Braam J.
      Plant J. 9:879-889(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Columbia.
    2. "Expression of endoxyloglucan transferase genes in acaulis mutants of Arabidopsis."
      Akamatsu T., Hanzawa Y., Ohtake Y., Takahashi T., Nishitani K., Komeda Y.
      Plant Physiol. 121:715-721(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
      Strain: cv. Columbia.
    3. Okamoto S., Kamimai T., Nishitani K.
      Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Columbia.
    4. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    5. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    6. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    7. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    8. "The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis: current perspectives and a new unifying nomenclature."
      Rose J.K.C., Braam J., Fry S.C., Nishitani K.
      Plant Cell Physiol. 43:1421-1435(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NOMENCLATURE.

    Entry informationi

    Entry nameiXTH28_ARATH
    AccessioniPrimary (citable) accession number: Q38909
    Secondary accession number(s): O04750
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2003
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 118 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In contrast to group 1 and group 2 endotransglucosylase/hydrolase proteins, it may not contain the ligase activity, and may catalyze endohydrolysis xyloglucan polymers only.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3