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Q38909 (XTH28_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable xyloglucan endotransglucosylase/hydrolase protein 28

Short name=At-XTH28
Short name=XTH-28
EC=2.4.1.207
Gene names
Name:XTH28
Synonyms:EXGT-A2, XTR2
Ordered Locus Names:At1g14720
ORF Names:F10B6.12, F10B6.29
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length332 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues By similarity.

Catalytic activity

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.

Subcellular location

Secretedcell wall Probable. Secretedextracellular spaceapoplast Probable.

Tissue specificity

Expressed in 7 day old seedlings, roots, rosette leaves, internodes between nodes bearing axillary shoots, nodes bearing flowers, flower buds and siliques. Ref.2

Post-translational modification

Contains at least one intrachain disulfide bond essential for its enzymatic activity By similarity.

Miscellaneous

In contrast to group 1 and group 2 endotransglucosylase/hydrolase proteins, it may not contain the ligase activity, and may catalyze endohydrolysis xyloglucan polymers only.

Sequence similarities

Belongs to the glycosyl hydrolase 16 family. XTH group 3 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Chain23 – 332310Probable xyloglucan endotransglucosylase/hydrolase protein 28
PRO_0000011828

Sites

Active site1081Nucleophile By similarity
Active site1121Proton donor By similarity

Amino acid modifications

Glycosylation1311N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q38909 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: AF8242902A0CA191

FASTA33238,252
        10         20         30         40         50         60 
MGFITRFLVF MSLFTSLVSG FALQKLPLIQ FDEGYTQLFG DQNLIVHRDG KSVRLTLDER 

        70         80         90        100        110        120 
TGSGFVSNDI YLHGFFSSSI KLPADYSAGV VIAFYLSNGD LYEKNHDEID FEFLGNIRGR 

       130        140        150        160        170        180 
EWRIQTNIYG NGSTHLGREE RYNLWFDPTE DFHQYSILWS LSHIIFYVDN VPIREVKRTA 

       190        200        210        220        230        240 
SMGGDFPAKP MSLYSTIWDG SKWATDGGKY GVNYKYAPYV SQFTDLILHG CAVDPTEKFP 

       250        260        270        280        290        300 
SCKDEAVQNL RLASEITESQ RNKMEIFRQK HMTYSYCYDH MRYKVVLSEC VVNPAEAKRL 

       310        320        330 
RVYDPVTFGG IPHGHRRGKH RSRSRLARTE SI 

« Hide

References

« Hide 'large scale' references
[1]"The Arabidopsis XET-related gene family: environmental and hormonal regulation of expression."
Xu W., Campbell P., Vargheese A.K., Braam J.
Plant J. 9:879-889(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
[2]"Expression of endoxyloglucan transferase genes in acaulis mutants of Arabidopsis."
Akamatsu T., Hanzawa Y., Ohtake Y., Takahashi T., Nishitani K., Komeda Y.
Plant Physiol. 121:715-721(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
Strain: cv. Columbia.
[3]Okamoto S., Kamimai T., Nishitani K.
Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
[4]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[5]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[6]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[7]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[8]"The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis: current perspectives and a new unifying nomenclature."
Rose J.K.C., Braam J., Fry S.C., Nishitani K.
Plant Cell Physiol. 43:1421-1435(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U43487 mRNA. Translation: AAB18366.1.
AF163820 Genomic DNA. Translation: AAD45124.1.
D63510 mRNA. Translation: BAA20290.1.
AC006917 Genomic DNA. Translation: AAF79246.1.
CP002684 Genomic DNA. Translation: AEE29214.1.
AF385714 mRNA. Translation: AAK60305.1.
AY085855 mRNA. Translation: AAM63068.1.
PIRS71224.
RefSeqNP_172925.1. NM_101341.3.
UniGeneAt.279.

3D structure databases

ProteinModelPortalQ38909.
SMRQ38909. Positions 31-290.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-8067472.
STRING3702.AT1G14720.1-P.

Protein family/group databases

CAZyGH16. Glycoside Hydrolase Family 16.

Proteomic databases

PRIDEQ38909.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G14720.1; AT1G14720.1; AT1G14720.
GeneID838037.
KEGGath:AT1G14720.

Organism-specific databases

GeneFarm2644. 265.
TAIRAT1G14720.

Phylogenomic databases

eggNOGCOG2273.
HOGENOMHOG000236368.
InParanoidQ38909.
KOK08235.
OMASHIIFYV.
PhylomeDBQ38909.

Enzyme and pathway databases

BioCycARA:AT1G14720-MONOMER.

Gene expression databases

ArrayExpressQ38909.
GenevestigatorQ38909.

Family and domain databases

Gene3D2.60.120.200. 1 hit.
InterProIPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000757. Glyco_hydro_16.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view]
PfamPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFPIRSF005604. XET. 1 hit.
SUPFAMSSF49899. SSF49899. 1 hit.
ProtoNetSearch...

Entry information

Entry nameXTH28_ARATH
AccessionPrimary (citable) accession number: Q38909
Secondary accession number(s): O04750
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: November 1, 1996
Last modified: May 14, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names