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Q38909

- XTH28_ARATH

UniProt

Q38909 - XTH28_ARATH

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Protein

Probable xyloglucan endotransglucosylase/hydrolase protein 28

Gene
XTH28, EXGT-A2, XTR2, At1g14720, F10B6.12, F10B6.29
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues By similarity.

Catalytic activityi

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei108 – 1081Nucleophile By similarity
Active sitei112 – 1121Proton donor By similarity

GO - Molecular functioni

  1. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro
  2. xyloglucan:xyloglucosyl transferase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellular glucan metabolic process Source: InterPro
  2. fruit development Source: TAIR
  3. stamen filament development Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Transferase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciARA:AT1G14720-MONOMER.

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable xyloglucan endotransglucosylase/hydrolase protein 28 (EC:2.4.1.207)
Short name:
At-XTH28
Short name:
XTH-28
Gene namesi
Name:XTH28
Synonyms:EXGT-A2, XTR2
Ordered Locus Names:At1g14720
ORF Names:F10B6.12, F10B6.29
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G14720.

Subcellular locationi

Secretedcell wall Inferred. Secretedextracellular spaceapoplast Inferred

GO - Cellular componenti

  1. apoplast Source: UniProtKB-SubCell
  2. cell wall Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222 Reviewed predictionAdd
BLAST
Chaini23 – 332310Probable xyloglucan endotransglucosylase/hydrolase protein 28PRO_0000011828Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi131 – 1311N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

Contains at least one intrachain disulfide bond essential for its enzymatic activity By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ38909.

Expressioni

Tissue specificityi

Expressed in 7 day old seedlings, roots, rosette leaves, internodes between nodes bearing axillary shoots, nodes bearing flowers, flower buds and siliques.1 Publication

Gene expression databases

ArrayExpressiQ38909.
GenevestigatoriQ38909.

Interactioni

Protein-protein interaction databases

MINTiMINT-8067472.
STRINGi3702.AT1G14720.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ38909.
SMRiQ38909. Positions 31-290.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2273.
HOGENOMiHOG000236368.
InParanoidiQ38909.
KOiK08235.
OMAiSHIIFYV.
PhylomeDBiQ38909.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000757. Glyco_hydro_16.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFiPIRSF005604. XET. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q38909-1 [UniParc]FASTAAdd to Basket

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MGFITRFLVF MSLFTSLVSG FALQKLPLIQ FDEGYTQLFG DQNLIVHRDG    50
KSVRLTLDER TGSGFVSNDI YLHGFFSSSI KLPADYSAGV VIAFYLSNGD 100
LYEKNHDEID FEFLGNIRGR EWRIQTNIYG NGSTHLGREE RYNLWFDPTE 150
DFHQYSILWS LSHIIFYVDN VPIREVKRTA SMGGDFPAKP MSLYSTIWDG 200
SKWATDGGKY GVNYKYAPYV SQFTDLILHG CAVDPTEKFP SCKDEAVQNL 250
RLASEITESQ RNKMEIFRQK HMTYSYCYDH MRYKVVLSEC VVNPAEAKRL 300
RVYDPVTFGG IPHGHRRGKH RSRSRLARTE SI 332
Length:332
Mass (Da):38,252
Last modified:November 1, 1996 - v1
Checksum:iAF8242902A0CA191
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U43487 mRNA. Translation: AAB18366.1.
AF163820 Genomic DNA. Translation: AAD45124.1.
D63510 mRNA. Translation: BAA20290.1.
AC006917 Genomic DNA. Translation: AAF79246.1.
CP002684 Genomic DNA. Translation: AEE29214.1.
AF385714 mRNA. Translation: AAK60305.1.
AY085855 mRNA. Translation: AAM63068.1.
PIRiS71224.
RefSeqiNP_172925.1. NM_101341.3.
UniGeneiAt.279.

Genome annotation databases

EnsemblPlantsiAT1G14720.1; AT1G14720.1; AT1G14720.
GeneIDi838037.
KEGGiath:AT1G14720.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U43487 mRNA. Translation: AAB18366.1 .
AF163820 Genomic DNA. Translation: AAD45124.1 .
D63510 mRNA. Translation: BAA20290.1 .
AC006917 Genomic DNA. Translation: AAF79246.1 .
CP002684 Genomic DNA. Translation: AEE29214.1 .
AF385714 mRNA. Translation: AAK60305.1 .
AY085855 mRNA. Translation: AAM63068.1 .
PIRi S71224.
RefSeqi NP_172925.1. NM_101341.3.
UniGenei At.279.

3D structure databases

ProteinModelPortali Q38909.
SMRi Q38909. Positions 31-290.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-8067472.
STRINGi 3702.AT1G14720.1-P.

Protein family/group databases

CAZyi GH16. Glycoside Hydrolase Family 16.

Proteomic databases

PRIDEi Q38909.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G14720.1 ; AT1G14720.1 ; AT1G14720 .
GeneIDi 838037.
KEGGi ath:AT1G14720.

Organism-specific databases

GeneFarmi 2644. 265.
TAIRi AT1G14720.

Phylogenomic databases

eggNOGi COG2273.
HOGENOMi HOG000236368.
InParanoidi Q38909.
KOi K08235.
OMAi SHIIFYV.
PhylomeDBi Q38909.

Enzyme and pathway databases

BioCyci ARA:AT1G14720-MONOMER.

Gene expression databases

ArrayExpressi Q38909.
Genevestigatori Q38909.

Family and domain databases

Gene3Di 2.60.120.200. 1 hit.
InterProi IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000757. Glyco_hydro_16.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view ]
Pfami PF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view ]
PIRSFi PIRSF005604. XET. 1 hit.
SUPFAMi SSF49899. SSF49899. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The Arabidopsis XET-related gene family: environmental and hormonal regulation of expression."
    Xu W., Campbell P., Vargheese A.K., Braam J.
    Plant J. 9:879-889(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. "Expression of endoxyloglucan transferase genes in acaulis mutants of Arabidopsis."
    Akamatsu T., Hanzawa Y., Ohtake Y., Takahashi T., Nishitani K., Komeda Y.
    Plant Physiol. 121:715-721(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  3. Okamoto S., Kamimai T., Nishitani K.
    Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  4. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  5. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  6. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  7. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  8. "The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis: current perspectives and a new unifying nomenclature."
    Rose J.K.C., Braam J., Fry S.C., Nishitani K.
    Plant Cell Physiol. 43:1421-1435(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE.

Entry informationi

Entry nameiXTH28_ARATH
AccessioniPrimary (citable) accession number: Q38909
Secondary accession number(s): O04750
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: November 1, 1996
Last modified: May 14, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

In contrast to group 1 and group 2 endotransglucosylase/hydrolase proteins, it may not contain the ligase activity, and may catalyze endohydrolysis xyloglucan polymers only.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi