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Protein

Probable xyloglucan endotransglucosylase/hydrolase protein 30

Gene

XTH30

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues (By similarity).By similarity

Catalytic activityi

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei109NucleophileBy similarity1
Active sitei113Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Transferase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciARA:AT1G32170-MONOMER.

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable xyloglucan endotransglucosylase/hydrolase protein 30 (EC:2.4.1.207)
Short name:
At-XTH30
Short name:
XTH-30
Gene namesi
Name:XTH30
Synonyms:XTR4
Ordered Locus Names:At1g32170
ORF Names:F3C3.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G32170.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000001183024 – 343Probable xyloglucan endotransglucosylase/hydrolase protein 30Add BLAST320

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi132N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Contains at least one intrachain disulfide bond essential for its enzymatic activity.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ38908.

Expressioni

Tissue specificityi

Predominantly expressed in green siliques.1 Publication

Gene expression databases

GenevisibleiQ38908. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G32170.1.

Structurei

3D structure databases

ProteinModelPortaliQ38908.
SMRiQ38908.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 224GH16PROSITE-ProRule annotationAdd BLAST201

Sequence similaritiesi

Contains 1 GH16 (glycosyl hydrolase family 16) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IITG. Eukaryota.
ENOG4111G24. LUCA.
HOGENOMiHOG000236368.
InParanoidiQ38908.
KOiK08235.
OMAiPFASEFS.
OrthoDBiEOG09360GX8.
PhylomeDBiQ38908.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR000757. GH16.
IPR010713. XET_C.
IPR016455. XTH.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFiPIRSF005604. XET. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS51762. GH16_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q38908-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKSSYNHIF ILILCLCLRS SSAFTNLNTL SFEESLSPLF GDANLVRSPD
60 70 80 90 100
DLSVRLLLDR YTGSGFISSN MYQHGFYSSM IKLPADYTAG VVVAFYTSNG
110 120 130 140 150
DVFEKTHDEL DIEFLGNIKG KPWRFQTNLY GNGSTHRGRE ERYRLWFDPS
160 170 180 190 200
KEFHRYSILW TPHKIIFWVD DVPIREVIRN DAMGADYPAK PMALYATIWD
210 220 230 240 250
ASDWATSGGK YKANYKFAPF VAEFKSFSLD GCSVDPIQEV PMDCSDSVDF
260 270 280 290 300
LESQDYSSIN SHQRAAMRRF RQRFMYYSYC YDTLRYPEPL PECVIVPAEK
310 320 330 340
DRFKETGRLK FGGTEARERR RNRRQQRRPE IEIESDPDDR KLL
Length:343
Mass (Da):39,888
Last modified:November 28, 2003 - v2
Checksum:iB4DD6A9CE8451CD2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti202S → F in AAM67311 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC084165 Genomic DNA. Translation: AAG23439.1.
CP002684 Genomic DNA. Translation: AEE31443.1.
AY062698 mRNA. Translation: AAL32776.1.
AY086104 mRNA. Translation: AAM67311.1.
U43486 mRNA. Translation: AAB18365.1.
PIRiB86446.
S71223.
RefSeqiNP_174496.1. NM_102950.4.
UniGeneiAt.10186.

Genome annotation databases

EnsemblPlantsiAT1G32170.1; AT1G32170.1; AT1G32170.
GeneIDi840109.
GrameneiAT1G32170.1; AT1G32170.1; AT1G32170.
KEGGiath:AT1G32170.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC084165 Genomic DNA. Translation: AAG23439.1.
CP002684 Genomic DNA. Translation: AEE31443.1.
AY062698 mRNA. Translation: AAL32776.1.
AY086104 mRNA. Translation: AAM67311.1.
U43486 mRNA. Translation: AAB18365.1.
PIRiB86446.
S71223.
RefSeqiNP_174496.1. NM_102950.4.
UniGeneiAt.10186.

3D structure databases

ProteinModelPortaliQ38908.
SMRiQ38908.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G32170.1.

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Proteomic databases

PaxDbiQ38908.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G32170.1; AT1G32170.1; AT1G32170.
GeneIDi840109.
GrameneiAT1G32170.1; AT1G32170.1; AT1G32170.
KEGGiath:AT1G32170.

Organism-specific databases

TAIRiAT1G32170.

Phylogenomic databases

eggNOGiENOG410IITG. Eukaryota.
ENOG4111G24. LUCA.
HOGENOMiHOG000236368.
InParanoidiQ38908.
KOiK08235.
OMAiPFASEFS.
OrthoDBiEOG09360GX8.
PhylomeDBiQ38908.

Enzyme and pathway databases

BioCyciARA:AT1G32170-MONOMER.

Miscellaneous databases

PROiQ38908.

Gene expression databases

GenevisibleiQ38908. AT.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR000757. GH16.
IPR010713. XET_C.
IPR016455. XTH.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFiPIRSF005604. XET. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS51762. GH16_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiXTH30_ARATH
AccessioniPrimary (citable) accession number: Q38908
Secondary accession number(s): Q8LDA9, Q9FVR2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: November 28, 2003
Last modified: November 30, 2016
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

In contrast to group 1 and group 2 endotransglucosylase/hydrolase proteins, it may not contain the ligase activity, and may catalyze endohydrolysis xyloglucan polymers only.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.