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Q38908

- XTH30_ARATH

UniProt

Q38908 - XTH30_ARATH

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Protein
Probable xyloglucan endotransglucosylase/hydrolase protein 30
Gene
XTH30, XTR4, At1g32170, F3C3.5
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues By similarity.

Catalytic activityi

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei109 – 1091Nucleophile By similarity
Active sitei113 – 1131Proton donor By similarity

GO - Molecular functioni

  1. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro
  2. xyloglucan:xyloglucosyl transferase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellular glucan metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Transferase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciARA:AT1G32170-MONOMER.

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable xyloglucan endotransglucosylase/hydrolase protein 30 (EC:2.4.1.207)
Short name:
At-XTH30
Short name:
XTH-30
Gene namesi
Name:XTH30
Synonyms:XTR4
Ordered Locus Names:At1g32170
ORF Names:F3C3.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G32170.

Subcellular locationi

Secretedcell wall Inferred. Secretedextracellular spaceapoplast Inferred

GO - Cellular componenti

  1. apoplast Source: UniProtKB-SubCell
  2. cell wall Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323 Reviewed prediction
Add
BLAST
Chaini24 – 343320Probable xyloglucan endotransglucosylase/hydrolase protein 30
PRO_0000011830Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi132 – 1321N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

Contains at least one intrachain disulfide bond essential for its enzymatic activity By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ38908.
PRIDEiQ38908.

Expressioni

Tissue specificityi

Predominantly expressed in green siliques.1 Publication

Gene expression databases

GenevestigatoriQ38908.

Structurei

3D structure databases

ProteinModelPortaliQ38908.
SMRiQ38908. Positions 25-293.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2273.
HOGENOMiHOG000236368.
InParanoidiQ38908.
KOiK08235.
OMAiFISSNMY.
PhylomeDBiQ38908.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000757. Glyco_hydro_16.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFiPIRSF005604. XET. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q38908-1 [UniParc]FASTAAdd to Basket

« Hide

MSKSSYNHIF ILILCLCLRS SSAFTNLNTL SFEESLSPLF GDANLVRSPD    50
DLSVRLLLDR YTGSGFISSN MYQHGFYSSM IKLPADYTAG VVVAFYTSNG 100
DVFEKTHDEL DIEFLGNIKG KPWRFQTNLY GNGSTHRGRE ERYRLWFDPS 150
KEFHRYSILW TPHKIIFWVD DVPIREVIRN DAMGADYPAK PMALYATIWD 200
ASDWATSGGK YKANYKFAPF VAEFKSFSLD GCSVDPIQEV PMDCSDSVDF 250
LESQDYSSIN SHQRAAMRRF RQRFMYYSYC YDTLRYPEPL PECVIVPAEK 300
DRFKETGRLK FGGTEARERR RNRRQQRRPE IEIESDPDDR KLL 343
Length:343
Mass (Da):39,888
Last modified:November 28, 2003 - v2
Checksum:iB4DD6A9CE8451CD2
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti202 – 2021S → F in AAM67311. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC084165 Genomic DNA. Translation: AAG23439.1.
CP002684 Genomic DNA. Translation: AEE31443.1.
AY062698 mRNA. Translation: AAL32776.1.
AY086104 mRNA. Translation: AAM67311.1.
U43486 mRNA. Translation: AAB18365.1.
PIRiB86446.
S71223.
RefSeqiNP_174496.1. NM_102950.3.
UniGeneiAt.10186.

Genome annotation databases

EnsemblPlantsiAT1G32170.1; AT1G32170.1; AT1G32170.
GeneIDi840109.
KEGGiath:AT1G32170.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC084165 Genomic DNA. Translation: AAG23439.1 .
CP002684 Genomic DNA. Translation: AEE31443.1 .
AY062698 mRNA. Translation: AAL32776.1 .
AY086104 mRNA. Translation: AAM67311.1 .
U43486 mRNA. Translation: AAB18365.1 .
PIRi B86446.
S71223.
RefSeqi NP_174496.1. NM_102950.3.
UniGenei At.10186.

3D structure databases

ProteinModelPortali Q38908.
SMRi Q38908. Positions 25-293.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH16. Glycoside Hydrolase Family 16.

Proteomic databases

PaxDbi Q38908.
PRIDEi Q38908.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G32170.1 ; AT1G32170.1 ; AT1G32170 .
GeneIDi 840109.
KEGGi ath:AT1G32170.

Organism-specific databases

TAIRi AT1G32170.

Phylogenomic databases

eggNOGi COG2273.
HOGENOMi HOG000236368.
InParanoidi Q38908.
KOi K08235.
OMAi FISSNMY.
PhylomeDBi Q38908.

Enzyme and pathway databases

BioCyci ARA:AT1G32170-MONOMER.

Gene expression databases

Genevestigatori Q38908.

Family and domain databases

Gene3Di 2.60.120.200. 1 hit.
InterProi IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000757. Glyco_hydro_16.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view ]
Pfami PF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view ]
PIRSFi PIRSF005604. XET. 1 hit.
SUPFAMi SSF49899. SSF49899. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "The Arabidopsis XET-related gene family: environmental and hormonal regulation of expression."
    Xu W., Campbell P., Vargheese A.K., Braam J.
    Plant J. 9:879-889(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-343.
    Strain: cv. Columbia.
  6. "A comprehensive expression analysis of all members of a gene family encoding cell-wall enzymes allowed us to predict cis-regulatory regions involved in cell-wall construction in specific organs of Arabidopsis."
    Yokoyama R., Nishitani K.
    Plant Cell Physiol. 42:1025-1033(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  7. "The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis: current perspectives and a new unifying nomenclature."
    Rose J.K.C., Braam J., Fry S.C., Nishitani K.
    Plant Cell Physiol. 43:1421-1435(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE.

Entry informationi

Entry nameiXTH30_ARATH
AccessioniPrimary (citable) accession number: Q38908
Secondary accession number(s): Q8LDA9, Q9FVR2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: November 28, 2003
Last modified: May 14, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

In contrast to group 1 and group 2 endotransglucosylase/hydrolase proteins, it may not contain the ligase activity, and may catalyze endohydrolysis xyloglucan polymers only.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi