Q38908 (XTH30_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable xyloglucan endotransglucosylase/hydrolase protein 30
Short name=At-XTH30
Short name=XTH-30
EC=2.4.1.207

Gene names

Name:	XTH30
Synonyms:	XTR4
Ordered Locus Names:	At1g32170
ORF Names:	F3C3.5

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues By similarity.
Catalytic activity	Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.
Subcellular location	Secreted › cell wall Probable. Secreted › extracellular space › apoplast Probable.
Tissue specificity	Predominantly expressed in green siliques. Ref.6
Post-translational modification	Contains at least one intrachain disulfide bond essential for its enzymatic activity By similarity.
Miscellaneous	In contrast to group 1 and group 2 endotransglucosylase/hydrolase proteins, it may not contain the ligase activity, and may catalyze endohydrolysis xyloglucan polymers only.
Sequence similarities	Belongs to the glycosyl hydrolase 16 family. XTH group 3 subfamily.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Apoplast Cell wall Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase Transferase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular glucan metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro xyloglucan:xyloglucosyl transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 343	320	Probable xyloglucan endotransglucosylase/hydrolase protein 30		PRO_0000011830
Sites
	Active site	109	1	Nucleophile By similarity
	Active site	113	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	132	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	202	1	S → F in AAM67311. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q38908 [UniParc]. Last modified November 28, 2003. Version 2. Checksum: B4DD6A9CE8451CD2	FASTA	343	39,888
	10 20 30 40 50 60 MSKSSYNHIF ILILCLCLRS SSAFTNLNTL SFEESLSPLF GDANLVRSPD DLSVRLLLDR 70 80 90 100 110 120 YTGSGFISSN MYQHGFYSSM IKLPADYTAG VVVAFYTSNG DVFEKTHDEL DIEFLGNIKG 130 140 150 160 170 180 KPWRFQTNLY GNGSTHRGRE ERYRLWFDPS KEFHRYSILW TPHKIIFWVD DVPIREVIRN 190 200 210 220 230 240 DAMGADYPAK PMALYATIWD ASDWATSGGK YKANYKFAPF VAEFKSFSLD GCSVDPIQEV 250 260 270 280 290 300 PMDCSDSVDF LESQDYSSIN SHQRAAMRRF RQRFMYYSYC YDTLRYPEPL PECVIVPAEK 310 320 330 340 DRFKETGRLK FGGTEARERR RNRRQQRRPE IEIESDPDDR KLL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"The Arabidopsis XET-related gene family: environmental and hormonal regulation of expression." Xu W., Campbell P., Vargheese A.K., Braam J. Plant J. 9:879-889(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-343. Strain: cv. Columbia.
[6]	"A comprehensive expression analysis of all members of a gene family encoding cell-wall enzymes allowed us to predict cis-regulatory regions involved in cell-wall construction in specific organs of Arabidopsis." Yokoyama R., Nishitani K. Plant Cell Physiol. 42:1025-1033(2001) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[7]	"The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis: current perspectives and a new unifying nomenclature." Rose J.K.C., Braam J., Fry S.C., Nishitani K. Plant Cell Physiol. 43:1421-1435(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE.
+	Additional computationally mapped references.

Web resources

XTH-World

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC084165 Genomic DNA. Translation: AAG23439.1. CP002684 Genomic DNA. Translation: AEE31443.1. AY062698 mRNA. Translation: AAL32776.1. AY086104 mRNA. Translation: AAM67311.1. U43486 mRNA. Translation: AAB18365.1.
IPI	IPI00519069.
PIR	B86446. S71223.
RefSeq	NP_174496.1. NM_102950.3.
UniGene	At.10186.
3D structure databases
ProteinModelPortal	Q38908.
SMR	Q38908. Positions 25-293.
ModBase	Search...
Protein family/group databases
CAZy	GH16. Glycoside Hydrolase Family 16.
Proteomic databases
PaxDb	Q38908.
PRIDE	Q38908.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G32170.1; AT1G32170.1; AT1G32170.
GeneID	840109.
KEGG	ath:AT1G32170.
Organism-specific databases
TAIR	At1g32170.
Phylogenomic databases
eggNOG	COG2273.
HOGENOM	HOG000236368.
InParanoid	Q38908.
KO	K08235.
OMA	DASTWAT.
PhylomeDB	Q38908.
ProtClustDB	CLSN2913586.
Gene expression databases
Genevestigator	Q38908.
GermOnline	AT1G32170. Arabidopsis thaliana.
Family and domain databases
Gene3D	2.60.120.200. 1 hit.
InterPro	IPR008985. ConA-like_lec_gl_sf. IPR013320. ConA-like_subgrp. IPR000757. Glyco_hydro_16. IPR016455. XET. IPR010713. XET_C. [Graphical view]
Pfam	PF00722. Glyco_hydro_16. 1 hit. PF06955. XET_C. 1 hit. [Graphical view]
PIRSF	PIRSF005604. XET. 1 hit.
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit.
PROSITE	PS01034. GLYCOSYL_HYDROL_F16. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XTH30_ARATH

Accession

Primary (citable) accession number: Q38908
Secondary accession number(s): Q8LDA9, Q9FVR2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	November 28, 2003
Last modified:	May 1, 2013
This is version 102 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program