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Q38907 (XTH25_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable xyloglucan endotransglucosylase/hydrolase protein 25

Short name=At-XTH25
Short name=XTH-25
EC=2.4.1.207
Gene names
Name:XTH25
Synonyms:EXGT-A5, XTR3
Ordered Locus Names:At5g57550
ORF Names:MUA2.12
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length284 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues By similarity.

Catalytic activity

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.

Subcellular location

Secretedcell wall Probable. Secretedextracellular spaceapoplast Probable.

Tissue specificity

Expressed in siliques. Not detected in other tested tissues. Ref.1

Induction

By auxin. Not induced following darkness. Ref.5

Post-translational modification

Contains at least one intrachain disulfide bond essential for its enzymatic activity By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 16 family. XTH group 2 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Potential
Chain28 – 284257Probable xyloglucan endotransglucosylase/hydrolase protein 25
PRO_0000011825

Sites

Active site1041Nucleophile By similarity
Active site1081Proton donor By similarity

Amino acid modifications

Glycosylation1121N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q38907 [UniParc].

Last modified November 28, 2003. Version 2.
Checksum: 9ADC65F0B0213F76

FASTA28432,573
        10         20         30         40         50         60 
MDRSTFILSL LFTLTVSTTT LFSPVFAGTF DTEFDITWGD GRGKVLNNGE LLTLSLDRAS 

        70         80         90        100        110        120 
GSGFQTKKEY LFGKIDMQLK LVPGNSAGTV TAYYLKSKGD TWDEIDFEFL GNLTGDPYTM 

       130        140        150        160        170        180 
HTNVYTQGKG DREQQFHLWF DPTADFHTYS VLWNPHHIVF MVDDIPVREF KNLQHMGIQY 

       190        200        210        220        230        240 
PKLQPMRLYS SLWNADQWAT RGGLVKTDWS KAPFTASYRN FRADACVSSG GRSSCPAGSP 

       250        260        270        280 
RWFSQRLDLT AEDKMRVVQR KYMIYNYCTD TKRFPQGFPK ECRH 

« Hide

References

« Hide 'large scale' references
[1]"Expression of endoxyloglucan transferase genes in acaulis mutants of Arabidopsis."
Akamatsu T., Hanzawa Y., Ohtake Y., Takahashi T., Nishitani K., Komeda Y.
Plant Physiol. 121:715-721(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
Strain: cv. Columbia.
[2]"Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence features of the regions of 1,381,565 bp covered by twenty one physically assigned P1 and TAC clones."
Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N., Tabata S.
DNA Res. 5:131-145(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"The Arabidopsis XET-related gene family: environmental and hormonal regulation of expression."
Xu W., Campbell P., Vargheese A.K., Braam J.
Plant J. 9:879-889(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 8-284, INDUCTION.
Strain: cv. Columbia.
[6]"The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis: current perspectives and a new unifying nomenclature."
Rose J.K.C., Braam J., Fry S.C., Nishitani K.
Plant Cell Physiol. 43:1421-1435(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF163823 Genomic DNA. Translation: AAD45127.1.
AB011482 Genomic DNA. Translation: BAB08790.1.
CP002688 Genomic DNA. Translation: AED96914.1.
AY125495 mRNA. Translation: AAM78087.1.
AY143939 mRNA. Translation: AAN28878.1.
U43485 mRNA. Translation: AAB18364.1.
PIRS71222.
RefSeqNP_568859.2. NM_125136.3.
UniGeneAt.7483.

3D structure databases

ProteinModelPortalQ38907.
SMRQ38907. Positions 30-283.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT5G57550.1-P.

Protein family/group databases

CAZyGH16. Glycoside Hydrolase Family 16.

Proteomic databases

PaxDbQ38907.
PRIDEQ38907.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G57550.1; AT5G57550.1; AT5G57550.
GeneID835859.
KEGGath:AT5G57550.

Organism-specific databases

TAIRAT5G57550.

Phylogenomic databases

eggNOGCOG2273.
HOGENOMHOG000236368.
InParanoidQ38907.
KOK08235.
OMANFRADAC.
PhylomeDBQ38907.

Enzyme and pathway databases

BioCycARA:AT5G57550-MONOMER.

Gene expression databases

GenevestigatorQ38907.

Family and domain databases

Gene3D2.60.120.200. 1 hit.
InterProIPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view]
PfamPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFPIRSF005604. XET. 1 hit.
SUPFAMSSF49899. SSF49899. 1 hit.
PROSITEPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameXTH25_ARATH
AccessionPrimary (citable) accession number: Q38907
Secondary accession number(s): Q9SEB0
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: November 28, 2003
Last modified: May 14, 2014
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names