Probable xyloglucan endotransglucosylase/hydrolase protein 25 precursor

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Q38907 (XTH25_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 103. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Probable xyloglucan endotransglucosylase/hydrolase protein 25
Short name=At-XTH25
Short name=XTH-25
EC=2.4.1.207

Gene names

Name:	XTH25
Synonyms:	EXGT-A5, XTR3
Ordered Locus Names:	At5g57550
ORF Names:	MUA2.12

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues By similarity.
Catalytic activity	Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.
Subcellular location	Secreted › cell wall Probable. Secreted › extracellular space › apoplast Probable.
Tissue specificity	Expressed in siliques. Not detected in other tested tissues. Ref.1
Induction	By auxin. Not induced following darkness. Ref.5
Post-translational modification	Contains at least one intrachain disulfide bond essential for its enzymatic activity By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 16 family. XTH group 2 subfamily.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Apoplast Cell wall Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase Transferase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular glucan metabolic process Inferred from electronic annotation. Source: InterPro plant-type cell wall biogenesis Inferred from sequence or structural similarity PubMed 11673616. Source: TAIR
Cellular_component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro xyloglucan:xyloglucosyl transferase activity Inferred from sequence or structural similarity PubMed 11673616. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 27

Potential

Chain

28 – 284

257

Probable xyloglucan endotransglucosylase/hydrolase protein 25

PRO_0000011825

Sites

Active site

104

Nucleophile By similarity

Active site

108

Proton donor By similarity

Amino acid modifications

Glycosylation

112

N-linked (GlcNAc...) Potential

Sequences

Sequence

Length

Mass (Da)

Tools

Q38907 [UniParc].

Last modified November 28, 2003. Version 2.
Checksum: 9ADC65F0B0213F76
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FASTA

284

32,573

        10         20         30         40         50         60 
MDRSTFILSL LFTLTVSTTT LFSPVFAGTF DTEFDITWGD GRGKVLNNGE LLTLSLDRAS 

        70         80         90        100        110        120 
GSGFQTKKEY LFGKIDMQLK LVPGNSAGTV TAYYLKSKGD TWDEIDFEFL GNLTGDPYTM 

       130        140        150        160        170        180 
HTNVYTQGKG DREQQFHLWF DPTADFHTYS VLWNPHHIVF MVDDIPVREF KNLQHMGIQY 

       190        200        210        220        230        240 
PKLQPMRLYS SLWNADQWAT RGGLVKTDWS KAPFTASYRN FRADACVSSG GRSSCPAGSP 

       250        260        270        280 
RWFSQRLDLT AEDKMRVVQR KYMIYNYCTD TKRFPQGFPK ECRH

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression of endoxyloglucan transferase genes in acaulis mutants of Arabidopsis." Akamatsu T., Hanzawa Y., Ohtake Y., Takahashi T., Nishitani K., Komeda Y. Plant Physiol. 121:715-721(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY. Strain: cv. Columbia.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence features of the regions of 1,381,565 bp covered by twenty one physically assigned P1 and TAC clones." Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N., Tabata S. DNA Res. 5:131-145(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"The Arabidopsis XET-related gene family: environmental and hormonal regulation of expression." Xu W., Campbell P., Vargheese A.K., Braam J. Plant J. 9:879-889(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 8-284, INDUCTION. Strain: cv. Columbia.
[6]	"The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis: current perspectives and a new unifying nomenclature." Rose J.K.C., Braam J., Fry S.C., Nishitani K. Plant Cell Physiol. 43:1421-1435(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE.
+	Additional computationally mapped references.

Web resources

XTH-World

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF163823 Genomic DNA. Translation: AAD45127.1. AB011482 Genomic DNA. Translation: BAB08790.1. CP002688 Genomic DNA. Translation: AED96914.1. AY125495 mRNA. Translation: AAM78087.1. AY143939 mRNA. Translation: AAN28878.1. U43485 mRNA. Translation: AAB18364.1.
IPI	IPI00547635.
PIR	S71222.
RefSeq	NP_568859.2. NM_125136.3.
UniGene	At.7483.
3D structure databases
ProteinModelPortal	Q38907.
SMR	Q38907. Positions 30-283.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT5G57550.1-P.
Protein family/group databases
CAZy	GH16. Glycoside Hydrolase Family 16.
Proteomic databases
PaxDb	Q38907.
PRIDE	Q38907.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G57550.1; AT5G57550.1; AT5G57550.
GeneID	835859.
KEGG	ath:AT5G57550.
Organism-specific databases
TAIR	At5g57550.
Phylogenomic databases
eggNOG	COG2273.
HOGENOM	HOG000236368.
InParanoid	Q38907.
KO	K08235.
OMA	NFRADAC.
PhylomeDB	Q38907.
ProtClustDB	CLSN2917879.
Gene expression databases
Genevestigator	Q38907.
GermOnline	AT5G57550. Arabidopsis thaliana.
Family and domain databases
Gene3D	2.60.120.200. 1 hit.
InterPro	IPR008985. ConA-like_lec_gl_sf. IPR013320. ConA-like_subgrp. IPR000757. Glyco_hydro_16. IPR008263. Glycoside_hydrolase_16_AS. IPR016455. XET. IPR010713. XET_C. [Graphical view]
Pfam	PF00722. Glyco_hydro_16. 1 hit. PF06955. XET_C. 1 hit. [Graphical view]
PIRSF	PIRSF005604. XET. 1 hit.
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit.
PROSITE	PS01034. GLYCOSYL_HYDROL_F16. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XTH25_ARATH

Accession

Primary (citable) accession number: Q38907
Secondary accession number(s): Q9SEB0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	November 28, 2003
Last modified:	May 1, 2013
This is version 103 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Amino acid modifications

Sequences

References

Web resources

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents