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Q38907

- XTH25_ARATH

UniProt

Q38907 - XTH25_ARATH

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Protein

Probable xyloglucan endotransglucosylase/hydrolase protein 25

Gene

XTH25

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues (By similarity).By similarity

Catalytic activityi

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei104 – 1041NucleophilePROSITE-ProRule annotation
Active sitei108 – 1081Proton donorPROSITE-ProRule annotation

GO - Molecular functioni

  1. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro
  2. xyloglucan:xyloglucosyl transferase activity Source: TAIR

GO - Biological processi

  1. cellular glucan metabolic process Source: InterPro
  2. cell wall organization Source: UniProtKB-KW
  3. plant-type cell wall biogenesis Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Transferase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciARA:AT5G57550-MONOMER.

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable xyloglucan endotransglucosylase/hydrolase protein 25 (EC:2.4.1.207)
Short name:
At-XTH25
Short name:
XTH-25
Gene namesi
Name:XTH25
Synonyms:EXGT-A5, XTR3
Ordered Locus Names:At5g57550
ORF Names:MUA2.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G57550.

Subcellular locationi

GO - Cellular componenti

  1. apoplast Source: UniProtKB-KW
  2. cell wall Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Sequence AnalysisAdd
BLAST
Chaini28 – 284257Probable xyloglucan endotransglucosylase/hydrolase protein 25PRO_0000011825Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi112 – 1121N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Contains at least one intrachain disulfide bond essential for its enzymatic activity.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ38907.
PRIDEiQ38907.

Expressioni

Tissue specificityi

Expressed in siliques. Not detected in other tested tissues.1 Publication

Inductioni

By auxin. Not induced following darkness.1 Publication

Gene expression databases

GenevestigatoriQ38907.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G57550.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ38907.
SMRiQ38907. Positions 30-283.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2273.
HOGENOMiHOG000236368.
InParanoidiQ38907.
KOiK08235.
OMAiNFRADAC.
PhylomeDBiQ38907.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFiPIRSF005604. XET. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q38907-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDRSTFILSL LFTLTVSTTT LFSPVFAGTF DTEFDITWGD GRGKVLNNGE
60 70 80 90 100
LLTLSLDRAS GSGFQTKKEY LFGKIDMQLK LVPGNSAGTV TAYYLKSKGD
110 120 130 140 150
TWDEIDFEFL GNLTGDPYTM HTNVYTQGKG DREQQFHLWF DPTADFHTYS
160 170 180 190 200
VLWNPHHIVF MVDDIPVREF KNLQHMGIQY PKLQPMRLYS SLWNADQWAT
210 220 230 240 250
RGGLVKTDWS KAPFTASYRN FRADACVSSG GRSSCPAGSP RWFSQRLDLT
260 270 280
AEDKMRVVQR KYMIYNYCTD TKRFPQGFPK ECRH
Length:284
Mass (Da):32,573
Last modified:November 28, 2003 - v2
Checksum:i9ADC65F0B0213F76
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF163823 Genomic DNA. Translation: AAD45127.1.
AB011482 Genomic DNA. Translation: BAB08790.1.
CP002688 Genomic DNA. Translation: AED96914.1.
AY125495 mRNA. Translation: AAM78087.1.
AY143939 mRNA. Translation: AAN28878.1.
U43485 mRNA. Translation: AAB18364.1.
PIRiS71222.
RefSeqiNP_568859.2. NM_125136.3.
UniGeneiAt.7483.

Genome annotation databases

EnsemblPlantsiAT5G57550.1; AT5G57550.1; AT5G57550.
GeneIDi835859.
KEGGiath:AT5G57550.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF163823 Genomic DNA. Translation: AAD45127.1 .
AB011482 Genomic DNA. Translation: BAB08790.1 .
CP002688 Genomic DNA. Translation: AED96914.1 .
AY125495 mRNA. Translation: AAM78087.1 .
AY143939 mRNA. Translation: AAN28878.1 .
U43485 mRNA. Translation: AAB18364.1 .
PIRi S71222.
RefSeqi NP_568859.2. NM_125136.3.
UniGenei At.7483.

3D structure databases

ProteinModelPortali Q38907.
SMRi Q38907. Positions 30-283.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT5G57550.1-P.

Protein family/group databases

CAZyi GH16. Glycoside Hydrolase Family 16.

Proteomic databases

PaxDbi Q38907.
PRIDEi Q38907.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G57550.1 ; AT5G57550.1 ; AT5G57550 .
GeneIDi 835859.
KEGGi ath:AT5G57550.

Organism-specific databases

TAIRi AT5G57550.

Phylogenomic databases

eggNOGi COG2273.
HOGENOMi HOG000236368.
InParanoidi Q38907.
KOi K08235.
OMAi NFRADAC.
PhylomeDBi Q38907.

Enzyme and pathway databases

BioCyci ARA:AT5G57550-MONOMER.

Gene expression databases

Genevestigatori Q38907.

Family and domain databases

Gene3Di 2.60.120.200. 1 hit.
InterProi IPR013320. ConA-like_dom.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view ]
Pfami PF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view ]
PIRSFi PIRSF005604. XET. 1 hit.
SUPFAMi SSF49899. SSF49899. 1 hit.
PROSITEi PS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Expression of endoxyloglucan transferase genes in acaulis mutants of Arabidopsis."
    Akamatsu T., Hanzawa Y., Ohtake Y., Takahashi T., Nishitani K., Komeda Y.
    Plant Physiol. 121:715-721(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence features of the regions of 1,381,565 bp covered by twenty one physically assigned P1 and TAC clones."
    Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N., Tabata S.
    DNA Res. 5:131-145(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "The Arabidopsis XET-related gene family: environmental and hormonal regulation of expression."
    Xu W., Campbell P., Vargheese A.K., Braam J.
    Plant J. 9:879-889(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 8-284, INDUCTION.
    Strain: cv. Columbia.
  6. "The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis: current perspectives and a new unifying nomenclature."
    Rose J.K.C., Braam J., Fry S.C., Nishitani K.
    Plant Cell Physiol. 43:1421-1435(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE.

Entry informationi

Entry nameiXTH25_ARATH
AccessioniPrimary (citable) accession number: Q38907
Secondary accession number(s): Q9SEB0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: November 28, 2003
Last modified: October 29, 2014
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3