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Protein

Probable xyloglucan endotransglucosylase/hydrolase protein 25

Gene

XTH25

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues (By similarity).By similarity

Catalytic activityi

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei104NucleophilePROSITE-ProRule annotation1
Active sitei108Proton donorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • cell wall organization Source: UniProtKB-KW
  • plant-type cell wall biogenesis Source: TAIR
  • xyloglucan metabolic process Source: InterPro

Keywordsi

Molecular functionGlycosidase, Hydrolase, Transferase
Biological processCell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciARA:AT5G57550-MONOMER.

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable xyloglucan endotransglucosylase/hydrolase protein 25 (EC:2.4.1.207)
Short name:
At-XTH25
Short name:
XTH-25
Gene namesi
Name:XTH25
Synonyms:EXGT-A5, XTR3
Ordered Locus Names:At5g57550
ORF Names:MUA2.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G57550.
TAIRilocus:2174597. AT5G57550.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Apoplast, Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
ChainiPRO_000001182528 – 284Probable xyloglucan endotransglucosylase/hydrolase protein 25Add BLAST257

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi112N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Contains at least one intrachain disulfide bond essential for its enzymatic activity.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ38907.

Expressioni

Tissue specificityi

Expressed in siliques. Not detected in other tested tissues.1 Publication

Inductioni

By auxin. Not induced following darkness.1 Publication

Gene expression databases

ExpressionAtlasiQ38907. baseline and differential.
GenevisibleiQ38907. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G57550.1.

Structurei

3D structure databases

ProteinModelPortaliQ38907.
SMRiQ38907.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 218GH16PROSITE-ProRule annotationAdd BLAST191

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410II9P. Eukaryota.
COG2273. LUCA.
HOGENOMiHOG000236368.
InParanoidiQ38907.
KOiK08235.
OMAiFNANICN.
OrthoDBiEOG09360GR5.
PhylomeDBiQ38907.

Family and domain databases

InterProiView protein in InterPro
IPR013320. ConA-like_dom_sf.
IPR000757. GH16.
IPR008263. GH16_AS.
IPR010713. XET_C.
IPR016455. XTH.
PfamiView protein in Pfam
PF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
PIRSFiPIRSF005604. XET. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiView protein in PROSITE
PS01034. GH16_1. 1 hit.
PS51762. GH16_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q38907-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDRSTFILSL LFTLTVSTTT LFSPVFAGTF DTEFDITWGD GRGKVLNNGE
60 70 80 90 100
LLTLSLDRAS GSGFQTKKEY LFGKIDMQLK LVPGNSAGTV TAYYLKSKGD
110 120 130 140 150
TWDEIDFEFL GNLTGDPYTM HTNVYTQGKG DREQQFHLWF DPTADFHTYS
160 170 180 190 200
VLWNPHHIVF MVDDIPVREF KNLQHMGIQY PKLQPMRLYS SLWNADQWAT
210 220 230 240 250
RGGLVKTDWS KAPFTASYRN FRADACVSSG GRSSCPAGSP RWFSQRLDLT
260 270 280
AEDKMRVVQR KYMIYNYCTD TKRFPQGFPK ECRH
Length:284
Mass (Da):32,573
Last modified:November 28, 2003 - v2
Checksum:i9ADC65F0B0213F76
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF163823 Genomic DNA. Translation: AAD45127.1.
AB011482 Genomic DNA. Translation: BAB08790.1.
CP002688 Genomic DNA. Translation: AED96914.1.
AY125495 mRNA. Translation: AAM78087.1.
AY143939 mRNA. Translation: AAN28878.1.
U43485 mRNA. Translation: AAB18364.1.
PIRiS71222.
RefSeqiNP_568859.2. NM_125136.4.
UniGeneiAt.7483.

Genome annotation databases

EnsemblPlantsiAT5G57550.1; AT5G57550.1; AT5G57550.
GeneIDi835859.
GrameneiAT5G57550.1; AT5G57550.1; AT5G57550.
KEGGiath:AT5G57550.

Similar proteinsi

Entry informationi

Entry nameiXTH25_ARATH
AccessioniPrimary (citable) accession number: Q38907
Secondary accession number(s): Q9SEB0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: November 28, 2003
Last modified: November 22, 2017
This is version 130 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families