Q388M1 (GSK3B_TRYB2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycogen synthase kinase 3
Short name=GSK-3 short
EC=2.7.11.26

Gene names

Name:	GSK3
ORF Names:	Tb927.10.13780

Organism

Trypanosoma brucei brucei (strain 927/4 GUTat10.1) [Reference proteome]

Taxonomic identifier

999953 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Euglenozoa › Kinetoplastida › Trypanosomatidae › Trypanosoma › Trypanozoon › Trypanosoma brucei › Trypanosoma brucei brucei

Protein attributes

Sequence length	352 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + [tau protein] = ADP + [tau protein] phosphate. Ref.2
Subunit structure	Inhibited by cyclin kinase 2 (CDK2) inhibitors, including GW8510. Ref.2
Miscellaneous	Was identified as a drug target for the treatment of African sleeping sickness. RNA interference leads to growth arrest and altered parasite morphology, demonstrating requirement for cell survival. Ref.2
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily. Contains 1 protein kinase domain.
Biophysicochemical properties	Kinetic parameters: KM=4.5 µM for ATP Ref.2

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW tau-protein kinase activity Inferred from direct assay Ref.2. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 352	352	Glycogen synthase kinase 3		PRO_0000393864
Regions
	Domain	20 – 310	291	Protein kinase
	Nucleotide binding	26 – 34	9	ATP By similarity UniProtKB P28523
Sites
	Active site	152	1	Proton acceptor By similarity UniProtKB P28523
	Binding site	49	1	ATP By similarity UniProtKB P28523

Sequences

	Sequence		Length	Mass (Da)
	Q388M1 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: 7613B9C53B119A4B	FASTA	352	40,321
	10 20 30 40 50 60 MSLNLTDAAD DRSYKEMEKY TVERVAGQGT FGTVQLARDK STGSLVAIKK VIQDPRFKNR 70 80 90 100 110 120 ELQIMQHLAR LRHPNIVMLK NYFYTVGGEG RRNDVYLNVV MEFVPETLHR TCRNYYRRMT 130 140 150 160 170 180 NPPLILVKVF MFQLLRSIAC LHIPVINICH RDIKPHNVLV DEQTGELKLC DFGSAKRLAA 190 200 210 220 230 240 DEPNVAYICS RYYRAPELIF GNQFYTTAVD IWSVGCIFAE MLLGEPIFCG ENTSGQLREI 250 260 270 280 290 300 VKILGKPTKE ELHKLNGSST EINANAKATP WENVFKQPLP AEVYDLCGKI FKYVPDQRIT 310 320 330 340 350 PLDALCHPFF NELREPTTKL PSGNPLPAHL YQFTPDEVEA MTEAQREYLL KK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome of the African trypanosome Trypanosoma brucei." Berriman M., Ghedin E., Hertz-Fowler C., Blandin G., Renauld H., Bartholomeu D.C., Lennard N.J., Caler E., Hamlin N.E., Haas B., Bohme U., Hannick L., Aslett M.A., Shallom J., Marcello L., Hou L., Wickstead B., Alsmark U.C.M. , Arrowsmith C., Atkin R.J., Barron A.J., Bringaud F., Brooks K., Carrington M., Cherevach I., Chillingworth T.J., Churcher C., Clark L.N., Corton C.H., Cronin A., Davies R.M., Doggett J., Djikeng A., Feldblyum T., Field M.C., Fraser A., Goodhead I., Hance Z., Harper D., Harris B.R., Hauser H., Hostetler J., Ivens A., Jagels K., Johnson D., Johnson J., Jones K., Kerhornou A.X., Koo H., Larke N., Landfear S., Larkin C., Leech V., Line A., Lord A., Macleod A., Mooney P.J., Moule S., Martin D.M., Morgan G.W., Mungall K., Norbertczak H., Ormond D., Pai G., Peacock C.S., Peterson J., Quail M.A., Rabbinowitsch E., Rajandream M.A., Reitter C., Salzberg S.L., Sanders M., Schobel S., Sharp S., Simmonds M., Simpson A.J., Tallon L., Turner C.M., Tait A., Tivey A.R., Van Aken S., Walker D., Wanless D., Wang S., White B., White O., Whitehead S., Woodward J., Wortman J., Adams M.D., Embley T.M., Gull K., Ullu E., Barry J.D., Fairlamb A.H., Opperdoes F., Barrell B.G., Donelson J.E., Hall N., Fraser C.M., Melville S.E., El-Sayed N.M.A. Science 309:416-422(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 927/4 GUTat10.1.
[2]	"Glycogen synthase kinase 3 is a potential drug target for African trypanosomiasis therapy." Ojo K.K., Gillespie J.R., Riechers A.J., Napuli A.J., Verlinde C.L., Buckner F.S., Gelb M.H., Domostoj M.M., Wells S.J., Scheer A., Wells T.N., Van Voorhis W.C. Antimicrob. Agents Chemother. 52:3710-3717(2008) [PubMed] [Europe PMC] [Abstract] Cited for: CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, INHIBITION BY DRUGS, IDENTIFICATION AS A DRUG TARGET. Strain: 427.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CM000208 Genomic DNA. Translation: EAN78749.1.
RefSeq	XP_827861.1. XM_822768.1.
3D structure databases
ProteinModelPortal	Q388M1.
SMR	Q388M1. Positions 18-339.
ModBase	Search...
Protein-protein interaction databases
STRING	5691.Tb10.61.3140.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	EAN78749; EAN78749; Tb10.61.3140.
GeneID	3661993.
KEGG	tbr:Tb10.61.3140.
Phylogenomic databases
eggNOG	COG0515.
KO	K00870.
OMA	FGNLKLP.
ProtClustDB	CLSZ2444084.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSK3B_TRYB2

Accession

Primary (citable) accession number: Q388M1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 20, 2010
Last sequence update:	November 22, 2005
Last modified:	May 1, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)