Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q388M1 (GSK3B_TRYB2) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glycogen synthase kinase 3

Short name=GSK-3 short
EC=2.7.11.26
Gene names
Name:GSK3
ORF Names:Tb927.10.13780
OrganismTrypanosoma brucei brucei (strain 927/4 GUTat10.1) [Reference proteome]
Taxonomic identifier999953 [NCBI]
Taxonomic lineageEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosoma

Protein attributes

Sequence length352 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + [tau protein] = ADP + [tau protein] phosphate. Ref.2

Subunit structure

Inhibited by cyclin kinase 2 (CDK2) inhibitors, including GW8510. Ref.2

Miscellaneous

Was identified as a drug target for the treatment of African sleeping sickness. RNA interference leads to growth arrest and altered parasite morphology, demonstrating requirement for cell survival. Ref.2

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.

Contains 1 protein kinase domain.

Biophysicochemical properties

Kinetic parameters:

KM=4.5 µM for ATP Ref.2

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 352352Glycogen synthase kinase 3
PRO_0000393864

Regions

Domain20 – 310291Protein kinase
Nucleotide binding26 – 349ATP By similarity UniProtKB P28523

Sites

Active site1521Proton acceptor By similarity UniProtKB P28523
Binding site491ATP By similarity UniProtKB P28523

Sequences

Sequence LengthMass (Da)Tools
Q388M1 [UniParc].

Last modified November 22, 2005. Version 1.
Checksum: 7613B9C53B119A4B

FASTA35240,321
        10         20         30         40         50         60 
MSLNLTDAAD DRSYKEMEKY TVERVAGQGT FGTVQLARDK STGSLVAIKK VIQDPRFKNR 

        70         80         90        100        110        120 
ELQIMQHLAR LRHPNIVMLK NYFYTVGGEG RRNDVYLNVV MEFVPETLHR TCRNYYRRMT 

       130        140        150        160        170        180 
NPPLILVKVF MFQLLRSIAC LHIPVINICH RDIKPHNVLV DEQTGELKLC DFGSAKRLAA 

       190        200        210        220        230        240 
DEPNVAYICS RYYRAPELIF GNQFYTTAVD IWSVGCIFAE MLLGEPIFCG ENTSGQLREI 

       250        260        270        280        290        300 
VKILGKPTKE ELHKLNGSST EINANAKATP WENVFKQPLP AEVYDLCGKI FKYVPDQRIT 

       310        320        330        340        350 
PLDALCHPFF NELREPTTKL PSGNPLPAHL YQFTPDEVEA MTEAQREYLL KK 

« Hide

References

« Hide 'large scale' references
[1]"The genome of the African trypanosome Trypanosoma brucei."
Berriman M., Ghedin E., Hertz-Fowler C., Blandin G., Renauld H., Bartholomeu D.C., Lennard N.J., Caler E., Hamlin N.E., Haas B., Bohme U., Hannick L., Aslett M.A., Shallom J., Marcello L., Hou L., Wickstead B., Alsmark U.C.M. expand/collapse author list , Arrowsmith C., Atkin R.J., Barron A.J., Bringaud F., Brooks K., Carrington M., Cherevach I., Chillingworth T.J., Churcher C., Clark L.N., Corton C.H., Cronin A., Davies R.M., Doggett J., Djikeng A., Feldblyum T., Field M.C., Fraser A., Goodhead I., Hance Z., Harper D., Harris B.R., Hauser H., Hostetler J., Ivens A., Jagels K., Johnson D., Johnson J., Jones K., Kerhornou A.X., Koo H., Larke N., Landfear S., Larkin C., Leech V., Line A., Lord A., Macleod A., Mooney P.J., Moule S., Martin D.M., Morgan G.W., Mungall K., Norbertczak H., Ormond D., Pai G., Peacock C.S., Peterson J., Quail M.A., Rabbinowitsch E., Rajandream M.A., Reitter C., Salzberg S.L., Sanders M., Schobel S., Sharp S., Simmonds M., Simpson A.J., Tallon L., Turner C.M., Tait A., Tivey A.R., Van Aken S., Walker D., Wanless D., Wang S., White B., White O., Whitehead S., Woodward J., Wortman J., Adams M.D., Embley T.M., Gull K., Ullu E., Barry J.D., Fairlamb A.H., Opperdoes F., Barrell B.G., Donelson J.E., Hall N., Fraser C.M., Melville S.E., El-Sayed N.M.A.
Science 309:416-422(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 927/4 GUTat10.1.
[2]"Glycogen synthase kinase 3 is a potential drug target for African trypanosomiasis therapy."
Ojo K.K., Gillespie J.R., Riechers A.J., Napuli A.J., Verlinde C.L., Buckner F.S., Gelb M.H., Domostoj M.M., Wells S.J., Scheer A., Wells T.N., Van Voorhis W.C.
Antimicrob. Agents Chemother. 52:3710-3717(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, INHIBITION BY DRUGS, IDENTIFICATION AS A DRUG TARGET.
Strain: 427.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CM000208 Genomic DNA. Translation: EAN78749.1.
RefSeqXP_827861.1. XM_822768.1.

3D structure databases

ProteinModelPortalQ388M1.
SMRQ388M1. Positions 18-339.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5691.Tb10.61.3140.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsEAN78749; EAN78749; Tb10.61.3140.
GeneID3661993.
KEGGtbr:Tb10.61.3140.

Phylogenomic databases

eggNOGCOG0515.
KOK00870.
OMAFGNLKLP.
ProtClustDBCLSZ2444084.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGSK3B_TRYB2
AccessionPrimary (citable) accession number: Q388M1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 20, 2010
Last sequence update: November 22, 2005
Last modified: April 16, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families