Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glycogen synthase kinase 3

Gene

GSK3

Organism
Trypanosoma brucei brucei (strain 927/4 GUTat10.1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + [tau protein] = ADP + [tau protein] phosphate.1 Publication

Kineticsi

  1. KM=4.5 µM for ATP1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei49 – 491ATPPROSITE-ProRule annotationBy similarity
    Active sitei152 – 1521Proton acceptorPROSITE-ProRule annotationBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi26 – 349ATPPROSITE-ProRule annotationBy similarity

    GO - Molecular functioni

    GO - Biological processi

    • growth Source: GeneDB
    • protein phosphorylation Source: UniProtKB
    Complete GO annotation...

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycogen synthase kinase 31 Publication (EC:2.7.11.26)
    Short name:
    GSK-3 short1 Publication
    Gene namesi
    Name:GSK31 Publication
    ORF Names:Tb927.10.13780
    OrganismiTrypanosoma brucei brucei (strain 927/4 GUTat10.1)
    Taxonomic identifieri185431 [NCBI]
    Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosoma
    ProteomesiUP000008524 Componenti: Chromosome 10

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 352352Glycogen synthase kinase 3PRO_0000393864Add
    BLAST

    Interactioni

    Subunit structurei

    Inhibited by cyclin kinase 2 (CDK2) inhibitors, including GW8510.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ388M1.
    SMRiQ388M1. Positions 18-339.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini20 – 310291Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    InParanoidiQ388M1.
    KOiK00870.
    OMAiNAGERVQ.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR002290. Ser/Thr_dual-sp_kinase.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q388M1-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSLNLTDAAD DRSYKEMEKY TVERVAGQGT FGTVQLARDK STGSLVAIKK
    60 70 80 90 100
    VIQDPRFKNR ELQIMQHLAR LRHPNIVMLK NYFYTVGGEG RRNDVYLNVV
    110 120 130 140 150
    MEFVPETLHR TCRNYYRRMT NPPLILVKVF MFQLLRSIAC LHIPVINICH
    160 170 180 190 200
    RDIKPHNVLV DEQTGELKLC DFGSAKRLAA DEPNVAYICS RYYRAPELIF
    210 220 230 240 250
    GNQFYTTAVD IWSVGCIFAE MLLGEPIFCG ENTSGQLREI VKILGKPTKE
    260 270 280 290 300
    ELHKLNGSST EINANAKATP WENVFKQPLP AEVYDLCGKI FKYVPDQRIT
    310 320 330 340 350
    PLDALCHPFF NELREPTTKL PSGNPLPAHL YQFTPDEVEA MTEAQREYLL

    KK
    Length:352
    Mass (Da):40,321
    Last modified:November 22, 2005 - v1
    Checksum:i7613B9C53B119A4B
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CM000208 Genomic DNA. Translation: EAN78749.1.
    RefSeqiXP_827861.1. XM_822768.1.

    Genome annotation databases

    GeneIDi3661993.
    KEGGitbr:Tb10.61.3140.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CM000208 Genomic DNA. Translation: EAN78749.1.
    RefSeqiXP_827861.1. XM_822768.1.

    3D structure databases

    ProteinModelPortaliQ388M1.
    SMRiQ388M1. Positions 18-339.
    ModBaseiSearch...
    MobiDBiSearch...

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    GeneIDi3661993.
    KEGGitbr:Tb10.61.3140.

    Phylogenomic databases

    eggNOGiCOG0515.
    InParanoidiQ388M1.
    KOiK00870.
    OMAiNAGERVQ.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR002290. Ser/Thr_dual-sp_kinase.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "The genome of the African trypanosome Trypanosoma brucei."
      Berriman M., Ghedin E., Hertz-Fowler C., Blandin G., Renauld H., Bartholomeu D.C., Lennard N.J., Caler E., Hamlin N.E., Haas B., Bohme U., Hannick L., Aslett M.A., Shallom J., Marcello L., Hou L., Wickstead B., Alsmark U.C.M.
      , Arrowsmith C., Atkin R.J., Barron A.J., Bringaud F., Brooks K., Carrington M., Cherevach I., Chillingworth T.J., Churcher C., Clark L.N., Corton C.H., Cronin A., Davies R.M., Doggett J., Djikeng A., Feldblyum T., Field M.C., Fraser A., Goodhead I., Hance Z., Harper D., Harris B.R., Hauser H., Hostetler J., Ivens A., Jagels K., Johnson D., Johnson J., Jones K., Kerhornou A.X., Koo H., Larke N., Landfear S., Larkin C., Leech V., Line A., Lord A., Macleod A., Mooney P.J., Moule S., Martin D.M., Morgan G.W., Mungall K., Norbertczak H., Ormond D., Pai G., Peacock C.S., Peterson J., Quail M.A., Rabbinowitsch E., Rajandream M.A., Reitter C., Salzberg S.L., Sanders M., Schobel S., Sharp S., Simmonds M., Simpson A.J., Tallon L., Turner C.M., Tait A., Tivey A.R., Van Aken S., Walker D., Wanless D., Wang S., White B., White O., Whitehead S., Woodward J., Wortman J., Adams M.D., Embley T.M., Gull K., Ullu E., Barry J.D., Fairlamb A.H., Opperdoes F., Barrell B.G., Donelson J.E., Hall N., Fraser C.M., Melville S.E., El-Sayed N.M.A.
      Science 309:416-422(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 927/4 GUTat10.1Imported.
    2. Cited for: CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, INHIBITION BY DRUGS, IDENTIFICATION AS A DRUG TARGET.
      Strain: 427.

    Entry informationi

    Entry nameiGSK3B_TRYB2
    AccessioniPrimary (citable) accession number: Q388M1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 20, 2010
    Last sequence update: November 22, 2005
    Last modified: July 22, 2015
    This is version 66 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Miscellaneous

    Was identified as a drug target for the treatment of African sleeping sickness. RNA interference leads to growth arrest and altered parasite morphology, demonstrating requirement for cell survival.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.