UniProtKB - Q388A7 (LIPA_TRYB2)
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Protein
Lipoyl synthase, mitochondrial
Gene
Tb10.61.1530
Organism
Trypanosoma brucei brucei (strain 927/4 GUTat10.1)
Status
Functioni
Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation
Miscellaneous
This protein may be expected to contain an N-terminal transit peptide but none has been predicted.UniRule annotation
Catalytic activityi
Protein N6-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = protein N6-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotation
Cofactori
[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
: protein lipoylation via endogenous pathway Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].UniRule annotationProteins known to be involved in the 2 steps of the subpathway in this organism are:
- no protein annotated in this organism
- Lipoyl synthase, mitochondrial (Tb10.61.1530)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 125 | Iron-sulfur 1 (4Fe-4S)UniRule annotation | 1 | |
Metal bindingi | 130 | Iron-sulfur 1 (4Fe-4S)UniRule annotation | 1 | |
Metal bindingi | 136 | Iron-sulfur 1 (4Fe-4S)UniRule annotation | 1 | |
Metal bindingi | 159 | Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation | 1 | |
Metal bindingi | 163 | Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation | 1 | |
Metal bindingi | 166 | Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation | 1 |
GO - Molecular functioni
- 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
- iron ion binding Source: GeneDB
- lipoate synthase activity Source: GeneDB
GO - Biological processi
- lipoate biosynthetic process Source: GeneDB
- protein lipoylation Source: GO_Central
Keywordsi
Molecular function | Transferase |
Ligand | 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine |
Enzyme and pathway databases
UniPathwayi | UPA00538; UER00593 |
Names & Taxonomyi
Protein namesi | Recommended name: Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)Alternative name(s): Lipoate synthaseUniRule annotation Short name: LSUniRule annotation Short name: Lip-synUniRule annotation Lipoic acid synthaseUniRule annotation |
Gene namesi | ORF Names:Tb10.61.1530 |
Organismi | Trypanosoma brucei brucei (strain 927/4 GUTat10.1) |
Taxonomic identifieri | 185431 [NCBI] |
Taxonomic lineagei | Eukaryota › Euglenozoa › Kinetoplastida › Trypanosomatidae › Trypanosoma › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | TriTrypDB:Tb927.10.15010 |
Subcellular locationi
- Mitochondrion UniRule annotation
GO - Cellular componenti
- mitochondrion Source: GeneDB
Keywords - Cellular componenti
MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000398239 | 1 – 409 | Lipoyl synthase, mitochondrialAdd BLAST | 409 |
Proteomic databases
PaxDbi | Q388A7 |
Structurei
3D structure databases
ProteinModelPortali | Q388A7 |
SMRi | Q388A7 |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
HOGENOMi | HOG000235998 |
InParanoidi | Q388A7 |
KOi | K03644 |
OMAi | PYCDIDF |
Family and domain databases
Gene3Di | 3.20.20.701 hit |
HAMAPi | MF_00206 Lipoyl_synth, 1 hit |
InterProi | View protein in InterPro IPR013785 Aldolase_TIM IPR006638 Elp3/MiaB/NifB IPR031691 LIAS_N IPR003698 Lipoyl_synth IPR007197 rSAM |
Pfami | View protein in Pfam PF16881 LIAS_N, 1 hit PF04055 Radical_SAM, 1 hit |
PIRSFi | PIRSF005963 Lipoyl_synth, 1 hit |
SFLDi | SFLDG01058 lipoyl_synthase_like, 1 hit SFLDS00029 Radical_SAM, 1 hit |
SMARTi | View protein in SMART SM00729 Elp3, 1 hit |
TIGRFAMsi | TIGR00510 lipA, 1 hit |
i Sequence
Sequence statusi: Complete.
Q388A7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFQRWSFALC RPIVAAAQVS QQQVPPSEEP RNESGAANPP LVKEEFLRQF
60 70 80 90 100
RERLANDKTG RNSLEGFLDL PENLPPTAAS IGPLKRGKEP LPPWLKLKVP
110 120 130 140 150
MGASRQPRFN KIRRNMREKR LATVCEEAKC PNIGECWGGG DEEGDGTATA
160 170 180 190 200
TIMVMGAHCT RGCRFCSVMT SRTPPPLDPE EPRKTADAVA DMGVEYIVMT
210 220 230 240 250
MVDRDDLADG GAAHVVRCVT AVKERNPGLL LEALVGDFHG DLKLVEMVAG
260 270 280 290 300
SPLNVYAHNI ECVERITPNV RDRRASYRQS LKVLEHVNNF TKGAMLTKSS
310 320 330 340 350
IMLGLGEKEE EVRQTLRDLR TAGVSAVTLG QYLQPSRTRL KVSRYAHPKE
360 370 380 390 400
FEMWEKEALD MGFLYCASGP MVRSSYRAGE YYIKNILKQR ETVEAPSVSD
GGNEPKDSE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CM000208 Genomic DNA Translation: EAN78865.1 |
RefSeqi | XP_827977.1, XM_822884.1 |
Genome annotation databases
GeneDBi | Tb927.10.15010:pep |
GeneIDi | 3661775 |
KEGGi | tbr:Tb10.61.1530 |
Similar proteinsi
Entry informationi
Entry namei | LIPA_TRYB2 | |
Accessioni | Q388A7Primary (citable) accession number: Q388A7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 5, 2010 |
Last sequence update: | November 22, 2005 | |
Last modified: | April 25, 2018 | |
This is version 80 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) |