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Protein

Potassium channel AKT2/3

Gene

AKT2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Highly selective and weak inward-rectifying potassium channel. Plays a role in both loading and unloading potassium into/from the phloem sap. Seems to control sugar loading into phloem via a voltage-dependent process. Blocked by physiological concentrations of external calcium and by external acidification. May interact with the cytoskeleton or with regulatory proteins. Dephosphorylation by PP2CA not only leads to the inhibition of potassium currents but also to an increase of the voltage-dependence of the channel. Regulated by the CBL4/CIPK6 calcium sensor/protein kinase complex via a kinase interaction-dependent but phosphorylation-independent translocation of the channel to the plasma membrane.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi394 – 513cNMPAdd BLAST120

GO - Molecular functioni

GO - Biological processi

  • regulation of ion transmembrane transport Source: UniProtKB-KW
  • regulation of membrane potential Source: TAIR
  • response to abscisic acid Source: TAIR

Keywordsi

Molecular functionIon channel, Potassium channel, Voltage-gated channel
Biological processIon transport, Potassium transport, Transport
LigandPotassium

Protein family/group databases

TCDBi1.A.1.4.6 the voltage-gated ion channel (vic) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium channel AKT2/3
Gene namesi
Name:AKT2
Synonyms:AKT3
Ordered Locus Names:At4g22200
ORF Names:T10I14.30
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G22200
TAIRilocus:2132065 AT4G22200

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 79CytoplasmicSequence analysisAdd BLAST79
Transmembranei80 – 100Helical; Name=Segment S1Sequence analysisAdd BLAST21
Topological domaini101 – 109ExtracellularSequence analysis9
Transmembranei110 – 130Helical; Name=Segment S2Sequence analysisAdd BLAST21
Topological domaini131 – 153CytoplasmicSequence analysisAdd BLAST23
Transmembranei154 – 174Helical; Name=Segment S3Sequence analysisAdd BLAST21
Topological domaini175 – 183ExtracellularSequence analysis9
Transmembranei184 – 204Helical; Voltage-sensor; Name=Segment S4Sequence analysisAdd BLAST21
Topological domaini205 – 218CytoplasmicSequence analysisAdd BLAST14
Transmembranei219 – 239Helical; Name=Segment S5Sequence analysisAdd BLAST21
Topological domaini240 – 265ExtracellularSequence analysisAdd BLAST26
Intramembranei266 – 285Pore-forming; Name=Segment H5Sequence analysisAdd BLAST20
Topological domaini286 – 288ExtracellularSequence analysis3
Transmembranei289 – 309Helical; Name=Segment S6Sequence analysisAdd BLAST21
Topological domaini310 – 802CytoplasmicSequence analysisAdd BLAST493

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Delayed development and flowering.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi243H → D: Abolishes the proton sensitivity; lack of susceptibility to extracellular potassium; when associated with E-286. 1 Publication1
Mutagenesisi286S → E: Abolishes the proton and the potassium sensitivity; lack of susceptibility to extracellular potassium; when associated with D-243. 1 Publication1
Mutagenesisi289I → R: No change in proton sensitivity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000541221 – 802Potassium channel AKT2/3Add BLAST802

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi179N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi254N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Dephosphorylated by PP2CA.

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ38898
PRIDEiQ38898

Expressioni

Tissue specificityi

Expressed mainly in the phloem tissues throughout the plant but also, at a lower level, in leaf epiderm, mesophyll and guard cells.4 Publications

Inductioni

In shoots, strongly induced by abscisic acid (ABA) treatment and reduced after NaCl treatment or potassium starvation.1 Publication

Gene expression databases

ExpressionAtlasiQ38898 baseline and differential
GenevisibleiQ38898 AT

Interactioni

Subunit structurei

The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming subunits. Interacts with the phosphatase PPC2A and the kinase CIPK6. May interact with AKT1, KAT1 and KAT3. Interacts with SLAC1 (PubMed:27002025).6 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi13600, 14 interactors
IntActiQ38898, 6 interactors
STRINGi3702.AT4G22200.1

Structurei

3D structure databases

ProteinModelPortaliQ38898
SMRiQ38898
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati540 – 569ANK 1Add BLAST30
Repeati573 – 602ANK 2Add BLAST30
Repeati606 – 636ANK 3Add BLAST31
Repeati637 – 666ANK 4Add BLAST30
Repeati670 – 699ANK 5Add BLAST30
Domaini725 – 802KHAPROSITE-ProRule annotationAdd BLAST78

Domaini

The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids. The pore-forming region H5 is enclosed by the transmembrane segments S5 and S6 in the Shaker-type (1P/6TM) and contains the GYGD signature motif which seems to be involved in potassium selectivity.
The KHA domain (rich in hydrophobic and acidic residues) present in the C-terminal part is likely to be important for tetramerization.

Sequence similaritiesi

Keywords - Domaini

ANK repeat, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0498 Eukaryota
ENOG410XPSE LUCA
HOGENOMiHOG000238230
InParanoidiQ38898
KOiK21867
OMAiQMDMVNL
OrthoDBiEOG0936023D
PhylomeDBiQ38898

Family and domain databases

CDDicd00204 ANK, 1 hit
cd00038 CAP_ED, 1 hit
Gene3Di1.25.40.20, 1 hit
2.60.120.10, 1 hit
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR018490 cNMP-bd-like
IPR000595 cNMP-bd_dom
IPR005821 Ion_trans_dom
IPR003938 K_chnl_volt-dep_EAG/ELK/ERG
IPR021789 KHA_dom
IPR014710 RmlC-like_jellyroll
PfamiView protein in Pfam
PF12796 Ank_2, 1 hit
PF00027 cNMP_binding, 1 hit
PF00520 Ion_trans, 1 hit
PF11834 KHA, 1 hit
PRINTSiPR01415 ANKYRIN
PR01463 EAGCHANLFMLY
SMARTiView protein in SMART
SM00248 ANK, 5 hits
SM00100 cNMP, 1 hit
SUPFAMiSSF48403 SSF48403, 1 hit
SSF51206 SSF51206, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 2 hits
PS50042 CNMP_BINDING_3, 1 hit
PS51490 KHA, 1 hit

Sequencei

Sequence statusi: Complete.

Q38898-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLKYSASHC NLSSDMKLRR FHQHRGKGRE EEYDASSLSL NNLSKLILPP
60 70 80 90 100
LGVASYNQNH IRSSGWIISP MDSRYRCWEF YMVLLVAYSA WVYPFEVAFL
110 120 130 140 150
NSSPKRNLCI ADNIVDLFFA VDIVLTFFVA YIDERTQLLV REPKQIAVRY
160 170 180 190 200
LSTWFLMDVA STIPFDAIGY LITGTSTLNI TCNLLGLLRF WRLRRVKHLF
210 220 230 240 250
TRLEKDIRYS YFWIRCFRLL SVTLFLVHCA GCSYYLIADR YPHQGKTWTD
260 270 280 290 300
AIPNFTETSL SIRYIAAIYW SITTMTTVGY GDLHASNTIE MVFITVYMLF
310 320 330 340 350
NLGLTAYLIG NMTNLVVEGT RRTMEFRNSI EAASNFVNRN RLPPRLKDQI
360 370 380 390 400
LAYMCLRFKA ESLNQQHLID QLPKSIYKSI CQHLFLPSVE KVYLFKGVSR
410 420 430 440 450
EILLLLVSKM KAEYIPPRED VIMQNEAPDD VYIIVSGEVE IIDSEMERES
460 470 480 490 500
VLGTLRCGDI FGEVGALCCR PQSYTFQTKS LSQLLRLKTS FLIETMQIKQ
510 520 530 540 550
QDNATMLKNF LQHHKKLSNL DIGDLKAQQN GENTDVVPPN IASNLIAVVT
560 570 580 590 600
TGNAALLDEL LKAKLSPDIT DSKGKTPLHV AASRGYEDCV LVLLKHGCNI
610 620 630 640 650
HIRDVNGNSA LWEAIISKHY EIFRILYHFA AISDPHIAGD LLCEAAKQNN
660 670 680 690 700
VEVMKALLKQ GLNVDTEDHH GVTALQVAMA EDQMDMVNLL ATNGADVVCV
710 720 730 740 750
NTHNEFTPLE KLRVVEEEEE EERGRVSIYR GHPLERRERS CNEAGKLILL
760 770 780 790 800
PPSLDDLKKI AGEKFGFDGS ETMVTNEDGA EIDSIEVIRD NDKLYFVVNK

II
Length:802
Mass (Da):91,308
Last modified:November 1, 1996 - v1
Checksum:i6F138F9149ED3CD7
GO

Sequence cautioni

The sequence AAA96153 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAA96154 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAA16770 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAB79175 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40154 mRNA Translation: AAA97865.1
U44744 Genomic DNA Translation: AAA96153.1 Different initiation.
U44745 mRNA Translation: AAA96154.1 Different initiation.
AL021712 Genomic DNA Translation: CAA16770.1 Different initiation.
AL161556 Genomic DNA Translation: CAB79175.1 Different initiation.
CP002687 Genomic DNA Translation: AEE84571.1
AK229735 mRNA Translation: BAF01572.1
PIRiS68699
RefSeqiNP_567651.1, NM_118342.3
UniGeneiAt.1970

Genome annotation databases

EnsemblPlantsiAT4G22200.1; AT4G22200.1; AT4G22200
GeneIDi828311
GrameneiAT4G22200.1; AT4G22200.1; AT4G22200
KEGGiath:AT4G22200

Similar proteinsi

Entry informationi

Entry nameiAKT2_ARATH
AccessioniPrimary (citable) accession number: Q38898
Secondary accession number(s): Q0WMS8, Q42408, Q9M0L6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 155 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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