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Protein

Transcriptional regulator SUPERMAN

Gene

SUP

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable transcriptional regulator considered as cadastral protein that acts indirectly to prevent the B class homeotic proteins APETALA3 and perhaps PISTILLATA from acting in the gynoecial whorl. Principal function is to balance cell proliferation in the third and fourth whorls of developing flowers thereby maintaining the boundary between stamens and carpels. May fulfill this role by repressing genes implicated in cell division. Plays equally a role in the determinacy of the floral meristem. Is also required for normal ovule development.5 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri47 – 6923C2H2-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: TAIR
  • metal ion binding Source: UniProtKB-KW
  • transcription factor activity, sequence-specific DNA binding Source: TAIR

GO - Biological processi

  • cell differentiation Source: UniProtKB-KW
  • flower development Source: TAIR
  • formation of organ boundary Source: TAIR
  • hormone-mediated signaling pathway Source: TAIR
  • regulation of cell proliferation Source: TAIR
  • regulation of cell size Source: TAIR
  • specification of floral organ identity Source: CACAO
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Repressor

Keywords - Biological processi

Differentiation, Flowering, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional regulator SUPERMAN
Gene namesi
Name:SUP
Synonyms:FLO10
Ordered Locus Names:At3g23130
ORF Names:K14B15.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G23130.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi63 – 631G → D in sup-3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 204204Transcriptional regulator SUPERMANPRO_0000047839Add
BLAST

Proteomic databases

PaxDbiQ38895.
PRIDEiQ38895.

Expressioni

Tissue specificityi

Expressed early in the third whorl, and later becomes localized at the boundary between the stamen and carpel whorls. Also detected in developing ovule primordia.

Inductioni

Repressed by KRYPTONITE/SUVH4, member of the histone H3-K9 methyltransferase family that contributes with other factors to the CpNpG methylation of the SUP gene resulting in its silencing. The alternative epigenetic states of the SUPERMAN locus have been called Clark Kent alleles. Positively regulated at an early stage of development by LEAFY and by B class homeotic proteins APETALA3 and PISTILLATA. Later expression is maintained by both the B class homeotic proteins and the C class homeotic protein AGAMOUS. These two steps of regulation require the intervention of additional factors.1 Publication

Gene expression databases

ExpressionAtlasiQ38895. differential.
GenevisibleiQ38895. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G23130.1.

Structurei

Secondary structure

1
204
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi44 – 485Combined sources
Turni50 – 523Combined sources
Beta strandi55 – 584Combined sources
Helixi59 – 679Combined sources
Turni74 – 774Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NJQNMR-A42-78[»]
2L1ONMR-A42-78[»]
ProteinModelPortaliQ38895.
SMRiQ38895. Positions 42-78.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ38895.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni153 – 20452Leucine-zipper-likeAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi195 – 1995EAR-like (transcriptional repression)

Domaini

Contains two overlapping leucine-zipper-like motifs at the C-terminal region, which might serve as a site for protein-protein interaction. In this domain, a slightly degenerated ERF-associated amphiphilic repression (EAR) motif seems to be involved in the activity of transcriptional repression.

Sequence similaritiesi

Contains 1 C2H2-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri47 – 6923C2H2-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG410J435. Eukaryota.
ENOG410Z0H6. LUCA.
HOGENOMiHOG000240643.
InParanoidiQ38895.
OMAiSHEMKNG.
PhylomeDBiQ38895.

Family and domain databases

InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]
PfamiPF13912. zf-C2H2_6. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 1 hit.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 1 hit.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q38895-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERSNSIELR NSFYGRARTS PWSYGDYDNC QQDHDYLLGF SWPPRSYTCS
60 70 80 90 100
FCKREFRSAQ ALGGHMNVHR RDRARLRLQQ SPSSSSTPSP PYPNPNYSYS
110 120 130 140 150
TMANSPPPHH SPLTLFPTLS PPSSPRYRAG LIRSLSPKSK HTPENACKTK
160 170 180 190 200
KSSLLVEAGE ATRFTSKDAC KILRNDEIIS LELEIGLINE SEQDLDLELR

LGFA
Length:204
Mass (Da):23,084
Last modified:November 1, 1996 - v1
Checksum:i2CF36E29A821F76E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38946 Genomic DNA. Translation: AAC49116.1.
AB025608 Genomic DNA. Translation: BAA95724.1.
CP002686 Genomic DNA. Translation: AEE76721.1.
DQ056605 mRNA. Translation: AAY78753.1.
PIRiS60325.
RefSeqiNP_188954.1. NM_113214.1.
UniGeneiAt.53448.

Genome annotation databases

EnsemblPlantsiAT3G23130.1; AT3G23130.1; AT3G23130.
GeneIDi821888.
GrameneiAT3G23130.1; AT3G23130.1; AT3G23130.
KEGGiath:AT3G23130.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38946 Genomic DNA. Translation: AAC49116.1.
AB025608 Genomic DNA. Translation: BAA95724.1.
CP002686 Genomic DNA. Translation: AEE76721.1.
DQ056605 mRNA. Translation: AAY78753.1.
PIRiS60325.
RefSeqiNP_188954.1. NM_113214.1.
UniGeneiAt.53448.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NJQNMR-A42-78[»]
2L1ONMR-A42-78[»]
ProteinModelPortaliQ38895.
SMRiQ38895. Positions 42-78.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G23130.1.

Proteomic databases

PaxDbiQ38895.
PRIDEiQ38895.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G23130.1; AT3G23130.1; AT3G23130.
GeneIDi821888.
GrameneiAT3G23130.1; AT3G23130.1; AT3G23130.
KEGGiath:AT3G23130.

Organism-specific databases

TAIRiAT3G23130.

Phylogenomic databases

eggNOGiENOG410J435. Eukaryota.
ENOG410Z0H6. LUCA.
HOGENOMiHOG000240643.
InParanoidiQ38895.
OMAiSHEMKNG.
PhylomeDBiQ38895.

Miscellaneous databases

EvolutionaryTraceiQ38895.
PROiQ38895.

Gene expression databases

ExpressionAtlasiQ38895. differential.
GenevisibleiQ38895. AT.

Family and domain databases

InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]
PfamiPF13912. zf-C2H2_6. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 1 hit.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 1 hit.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Role of SUPERMAN in maintaining Arabidopsis floral whorl boundaries."
    Sakai H., Medrano L.J., Meyerowitz E.M.
    Nature 378:199-203(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, MUTANT SUP-3.
    Strain: cv. Landsberg erecta.
  2. "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
    DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W., Redman J.C., Wu H.C., Utterback T., Town C.D.
    Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "The FLO10 gene product regulates the expression domain of homeotic genes AP3 and PI in Arabidopsis flowers."
    Schultz E.A., Pickett F.B., Haughn G.W.
    Plant Cell 3:1221-1237(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTANT FLO-10.
    Strain: cv. Columbia.
  6. "SUPERMAN, a regulator of floral homeotic genes in Arabidopsis."
    Bowman J.L., Sakai H., Jack T., Weigel D., Mayer U., Meyerowitz E.M.
    Development 114:599-615(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTANT SUP-1.
    Strain: cv. Landsberg erecta.
  7. "Regulation of SUP expression identifies multiple regulators involved in Arabidopsis floral meristem development."
    Sakai H., Krizek B.A., Jacobsen S.E., Meyerowitz E.M.
    Plant Cell 12:1607-1618(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, GENETIC REGULATION.
  8. "The SUPERMAN protein is an active repressor whose carboxy-terminal repression domain is required for the development of normal flowers."
    Hiratsu K., Ohta M., Matsui K., Ohme-Takagi M.
    FEBS Lett. 514:351-354(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION OF THE CARBOXY-TERMINAL REPRESSION DOMAIN.
  9. "Control of CpNpG DNA methylation by the KRYPTONITE histone H3 methyltransferase."
    Jackson J.P., Lindroth A.M., Cao X., Jacobsen S.E.
    Nature 416:556-560(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  10. "NMR structure of the single QALGGH zinc finger domain from the Arabidopsis thaliana SUPERMAN protein."
    Isernia C., Bucci E., Leone M., Zaccaro L., Di Lello P., Digilio G., Esposito S., Saviano M., Di Blasio B., Pedone C., Pedone P.V., Fattorusso R.
    ChemBioChem 4:171-180(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 41-79.

Entry informationi

Entry nameiSUP_ARATH
AccessioniPrimary (citable) accession number: Q38895
Secondary accession number(s): Q4PSN1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 9, 2003
Last sequence update: November 1, 1996
Last modified: February 17, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Mutations in SUP cause the ectopic expression of APETALA3 leading to the development of extra stamens at the expense of the central carpels of the flower.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.