Q38893 (ZDS_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Zeta-carotene desaturase, chloroplastic/chromoplastic
EC=1.3.5.6

Alternative name(s):
9,9'-di-cis-zeta-carotene desaturase
Carotene 7,8-desaturase

Gene names

Name:	ZDS1
Synonyms:	ZDS
Ordered Locus Names:	At3g04870
ORF Names:	T9J14.18

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	558 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the conversion of zeta-carotene to lycopene via the intermediary of neurosporene. It carries out two consecutive desaturations (introduction of double bonds) at positions C-7 and C-7'. Shows stereoselectivity toward trans C15-C15'zeta-carotene double bond. The zeta-carotene produced by the phytoene desaturase PDS has a C15-C15' double bond in the cis configuration and it requires isomerization before being recognized as substrate by ZDS. The main product is 7,9,7',9'-tetra-cis-lycopene (pro-lycopene). Ref.6
Catalytic activity	9,9'-di-cis-zeta-carotene + 2 quinone = 7,9,7',9'-tetra-cis-lycopene + 2 quinol. Ref.6
Cofactor	Lipophilic quinones such as decyl-plastoquinone or decyl-ubiquinone By similarity.
Pathway	Carotenoid biosynthesis; lycopene biosynthesis.
Subcellular location	Plastid › chloroplast. Plastid › chromoplast.
Sequence similarities	Belongs to the zeta carotene desaturase family.

Ontologies

Keywords
Biological process	Carotenoid biosynthesis
Cellular component	Chloroplast Chromoplast Plastid
Domain	Transit peptide
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	9,9'-di-cis-zeta-carotene desaturation to 7,9,7',9'-tetra-cis-lycopene Inferred from electronic annotation. Source: EC carotenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	chloroplast envelope Inferred from direct assay PubMed 12938931PubMed 20061580. Source: TAIR chromoplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	7,9,9'-tricis-neurosporene:quinone oxidoreductase activity Inferred from electronic annotation. Source: EC 9,9'-dicis-carotene:quinone oxidoreductase activity Inferred from electronic annotation. Source: EC carotene 7,8-desaturase activity Inferred from electronic annotation. Source: InterPro nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 27	27	Chloroplast and chromoplast Potential
	Chain	28 – 558	531	Zeta-carotene desaturase, chloroplastic/chromoplastic		PRO_0000041605
Experimental info
	Sequence conflict	9	1	A → P in AAA91161. Ref.1
	Sequence conflict	93	1	G → A in AAA91161. Ref.1
	Sequence conflict	172	1	N → S in AAA91161. Ref.1
	Sequence conflict	370	1	V → G in AAA91161. Ref.1
	Sequence conflict	370	1	V → G in AAF85796. Ref.2
	Sequence conflict	463	1	S → P in AAA91161. Ref.1
	Sequence conflict	470	1	W → C in AAA91161. Ref.1
	Sequence conflict	470	1	W → C in AAF85796. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q38893 [UniParc]. Last modified May 29, 2007. Version 3. Checksum: 120E0B0614FE0CB7	FASTA	558	61,634
	10 20 30 40 50 60 MASSVVFAAT GSLSVPPLKS RRFYVNSSLD SDVSDMSVNA PKGLFPPEPV PYKGPKLKVA 70 80 90 100 110 120 IIGAGLAGMS TAVELLDQGH EVDIYDSRTF IGGKVGSFVD RRGNHIEMGL HVFFGCYNNL 130 140 150 160 170 180 FRLMKKVGAE KNLLVKDHTH TFINKDGTIG ELDFRFPVGA PIHGIRAFLV TNQLKPYDKL 190 200 210 220 230 240 RNSLALALSP VVKALVDPDG AMRDIRNLDS ISFSDWFLSK GGTRASIQRM WDPVAYALGF 250 260 270 280 290 300 IDCDNMSARC MLTIFSLFAT KTEASLLRML KGSPDVYLSG PIKQYITDRG GRIHLRWGCR 310 320 330 340 350 360 EILYDKSADG ETYVTGLAIS KATNKKIVKA DVYVAACDVP GIKRLLPKEW RESRFFNDIY 370 380 390 400 410 420 ELEGVPVVTV QLRYNGWVTE LQDIELARQL KRAVGLDNLL YTPDADFSCF ADLALASPAD 430 440 450 460 470 480 YYIEGQGTLL QCVLTPGDPY MRMPNDKIIE KVAMQVTELF PSSRGLEVTW SSVVKIAQSL 490 500 510 520 530 540 YREAPGKDPF RPDQKTPIKN FFLAGSYTKQ DYIDSMEGAT LSGRQASSYI CDAGEELAEL 550 NKKLSSSATA VPDELSLV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of zeta-carotene desaturase from Arabidopsis." Scolnik P.A., Bartley G.E. Plant Gene Register PGR95-111 Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Gene structure and regulation of the carotenoid biosynthetic pathway in Arabidopsis." Giuliano G., Rosati C., Santangelo G. Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Two Arabidopsis thaliana carotene desaturases, phytoene desaturase and zeta-carotene desaturase, expressed in Escherichia coli, catalyze a poly-cis pathway to yield pro-lycopene." Bartley G.E., Scolnik P.A., Beyer P. Eur. J. Biochem. 259:396-403(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U38550 mRNA. Translation: AAA91161.1. AF121947 Genomic DNA. Translation: AAF85796.1. AC009465 Genomic DNA. Translation: AAG51402.1. CP002686 Genomic DNA. Translation: AEE74148.1. CP002686 Genomic DNA. Translation: AEE74149.1. AY059920 mRNA. Translation: AAL24402.1. AY072096 mRNA. Translation: AAL59918.1. AY096583 mRNA. Translation: AAM20233.1.
IPI	IPI00541621.
RefSeq	NP_187138.1. NM_111359.3. NP_974222.1. NM_202493.2.
UniGene	At.24092. At.71000.
3D structure databases
ProteinModelPortal	Q38893.
SMR	Q38893. Positions 55-132, 493-536.
ModBase	Search...
Proteomic databases
PaxDb	Q38893.
PRIDE	Q38893.
Protocols and materials databases
DNASU	819647.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G04870.1; AT3G04870.1; AT3G04870. AT3G04870.2; AT3G04870.2; AT3G04870.
GeneID	819647.
KEGG	ath:AT3G04870.
Organism-specific databases
TAIR	At3g04870.
Phylogenomic databases
eggNOG	COG3349.
HOGENOM	HOG000150110.
InParanoid	Q38893.
KO	K00514.
OMA	DGNHIEM.
PhylomeDB	Q38893.
ProtClustDB	PLN02487.
Enzyme and pathway databases
BioCyc	ARA:AT3G04870-MONOMER. MetaCyc:AT3G04870-MONOMER.
UniPathway	UPA00803.
Gene expression databases
ArrayExpress	Q38893.
Genevestigator	Q38893.
Family and domain databases
Gene3D	3.40.50.720. 3 hits.
InterPro	IPR002937. Amino_oxidase. IPR016040. NAD(P)-bd_dom. IPR014103. Zeta_caro_desat. [Graphical view]
Pfam	PF01593. Amino_oxidase. 1 hit. [Graphical view]
TIGRFAMs	TIGR02732. zeta_caro_desat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ZDS_ARATH

Accession

Primary (citable) accession number: Q38893
Secondary accession number(s): Q93YN2, Q9CAV3, Q9LLY1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 2, 2001
Last sequence update:	May 29, 2007
Last modified:	May 1, 2013
This is version 100 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program