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Q38890 (GUN25_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endoglucanase 25

EC=3.2.1.4
Alternative name(s):
Cellulase homolog OR16pep
Endo-1,4-beta glucanase 25
Protein KORRIGAN
Protein RADIALLY SWOLLEN 2
Gene names
Name:KOR
Synonyms:DEC, KOR1, RSW2
Ordered Locus Names:At5g49720
ORF Names:K2I5.8
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length621 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for cellulose microfibrils formation. Involved in cell wall assembly during cell elongation and cell plate maturation in cytokinesis. Required for secondary cell wall formation in the developing xylem. May cycle through different intracellular compartments, including plasma membrane. Ref.2 Ref.3 Ref.9 Ref.13 Ref.14

Catalytic activity

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Subcellular location

Cell membrane; Single-pass type II membrane protein. Note: Cell plate. Ref.2

Tissue specificity

Highly expressed in roots and stems, at intermediate levels in leaves and flowers, and at lower levels in siliques. Expressed in xylem (at protein level). Ref.2 Ref.3 Ref.13

Developmental stage

Expressed during hypocotyl elongation in the dark. Ref.2

Post-translational modification

Glycosylated. N-glycosylation of KOR in the endoplasmic reticulum followed by N-glycan modifications in the Golgi are essential for catalytic activity. Ref.15

Disruption phenotype

Plants are extremely dwarf and show severe abnormal morphology with incomplete cell walls, aberrant cell plates and multinucleated cells. Ref.2

Sequence similarities

Belongs to the glycosyl hydrolase 9 (cellulase E) family.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Cell wall biogenesis/degradation
Cellulose degradation
Polysaccharide degradation
   Cellular componentCell membrane
Membrane
   DomainSignal-anchor
Transmembrane
Transmembrane helix
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellulose biosynthetic process

Inferred from mutant phenotype PubMed 18583534. Source: TAIR

cellulose catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

cortical microtubule organization

Inferred from mutant phenotype PubMed 18583534. Source: TAIR

hyperosmotic salinity response

Inferred from mutant phenotype Ref.15. Source: TAIR

response to cytokinin

Inferred from mutant phenotype PubMed 12060228. Source: TAIR

shoot system development

Inferred from mutant phenotype PubMed 12060228. Source: TAIR

unidimensional cell growth

Inferred from mutant phenotype PubMed 10987560. Source: TAIR

   Cellular_componentGolgi apparatus

Inferred from direct assay Ref.14PubMed 22430844PubMed 22923678. Source: TAIR

cell plate

Inferred from direct assay Ref.3. Source: TAIR

early endosome

Inferred from direct assay Ref.14. Source: TAIR

endosome

Inferred from direct assay PubMed 22923678. Source: TAIR

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from direct assay Ref.10PubMed 17317660. Source: TAIR

trans-Golgi network

Inferred from direct assay PubMed 22923678. Source: TAIR

   Molecular_functioncellulase activity

Inferred from sequence or structural similarity Ref.2. Source: TAIR

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 621621Endoglucanase 25
PRO_0000249277

Regions

Topological domain1 – 7070Cytoplasmic Potential
Transmembrane71 – 9121Helical; Signal-anchor for type II membrane protein; Potential
Topological domain92 – 621530Extracellular Potential
Region48 – 492Polarized targeting signal 1 (PTS1)
Region59 – 624Polarized targeting signal 2 (PTS2)

Sites

Active site5131 By similarity
Active site5611 By similarity
Active site5701 By similarity

Amino acid modifications

Glycosylation1081N-linked (GlcNAc...) Potential
Glycosylation1331N-linked (GlcNAc...) Potential
Glycosylation2161N-linked (GlcNAc...) Potential
Glycosylation3241N-linked (GlcNAc...) Potential
Glycosylation3451N-linked (GlcNAc...) Potential
Glycosylation4081N-linked (GlcNAc...) Potential
Glycosylation4251N-linked (GlcNAc...) Potential
Glycosylation5671N-linked (GlcNAc...) Potential

Experimental info

Mutagenesis48 – 492LL → AA: Abolishes polarized targeting to cell plate.
Mutagenesis591Y → A: Abolishes polarized targeting to cell plate. Ref.3
Mutagenesis1831S → N in rsw2-3; decrease in cellulose production. Ref.9
Mutagenesis2501P → L in irx2-1; decrease in cellulose production. Ref.13
Mutagenesis3441G → R in rsw2-4; decrease in cellulose production. Ref.9
Mutagenesis4291G → R in rsw2-1 and rsw2-2; decrease in cellulose production. Ref.9
Mutagenesis5531P → L in irx2-2; decrease in cellulose production. Ref.13
Sequence conflict71W → S in AAM63370. Ref.7
Sequence conflict591Y → D in AAK59818. Ref.6
Sequence conflict3381W → R in AAM63370. Ref.7
Sequence conflict3971Y → C in AAK59818. Ref.6
Sequence conflict3971Y → C in AAN72232. Ref.6
Sequence conflict6051V → A in BAD94393. Ref.8

Sequences

Sequence LengthMass (Da)Tools
Q38890 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 798D8B8CC5DEBF42

FASTA62169,191
        10         20         30         40         50         60 
MYGRDPWGGP LEINTADSAT DDDRSRNLND LDRAALSRPL DETQQSWLLG PTEQKKKKYV 

        70         80         90        100        110        120 
DLGCIIVSRK IFVWTVGTLV AAALLAGFIT LIVKTVPRHH PKTPPPDNYT IALHKALKFF 

       130        140        150        160        170        180 
NAQKSGKLPK HNNVSWRGNS GLQDGKGETG SFYKDLVGGY YDAGDAIKFN FPMAYAMTML 

       190        200        210        220        230        240 
SWSVIEYSAK YEAAGELTHV KELIKWGTDY FLKTFNSTAD SIDDLVSQVG SGNTDDGNTD 

       250        260        270        280        290        300 
PNDHYCWMRP EDMDYKRPVT TCNGGCSDLA AEMAAALASA SIVFKDNKEY SKKLVHGAKV 

       310        320        330        340        350        360 
VYQFGRTRRG RYSAGTAESS KFYNSSMYWD EFIWGGAWMY YATGNVTYLN LITQPTMAKH 

       370        380        390        400        410        420 
AGAFWGGPYY GVFSWDNKLA GAQLLLSRLR LFLSPGYPYE EILRTFHNQT SIVMCSYLPI 

       430        440        450        460        470        480 
FNKFNRTNGG LIELNHGAPQ PLQYSVNAAF LATLYSDYLD AADTPGWYCG PNFYSTSVLR 

       490        500        510        520        530        540 
DFARSQIDYI LGKNPRKMSY VVGFGTKYPR HVHHRGASIP KNKVKYNCKG GWKWRDSKKP 

       550        560        570        580        590        600 
NPNTIEGAMV AGPDKRDGYR DVRMNYNYTE PTLAGNAGLV AALVALSGEE EATGKIDKNT 

       610        620 
IFSAVPPLFP TPPPPPAPWK P 

« Hide

References

« Hide 'large scale' references
[1]"An Arabidopsis thaliana cDNA homologous to cellulase."
Loebler M.
DNA Seq. 8:253-256(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
Tissue: Leaf.
[2]"A plasma membrane-bound putative endo-1,4-beta-D-glucanase is required for normal wall assembly and cell elongation in Arabidopsis."
Nicol F., His I., Jauneau A., Vernhettes S., Canut H., Hoefte H.
EMBO J. 17:5563-5576(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
Strain: cv. Columbia.
[3]"KORRIGAN, an Arabidopsis endo-1,4-beta-glucanase, localizes to the cell plate by polarized targeting and is essential for cytokinesis."
Zuo J., Niu Q.-W., Nishizawa N., Wu Y., Kost B., Chua N.-H.
Plant Cell 12:1137-1152(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF 48-LYS-LYS-49 AND TYR-59.
Strain: cv. Landsberg erecta.
[4]"Structural analysis of Arabidopsis thaliana chromosome 5. XI."
Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H., Tabata S.
Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[5]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[6]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[7]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[8]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 366-621.
Strain: cv. Columbia.
[9]"Temperature-sensitive alleles of RSW2 link the KORRIGAN endo-1,4-beta-glucanase to cellulose synthesis and cytokinesis in Arabidopsis."
Lane D.R., Wiedemeier A., Peng L., Hoefte H., Vernhettes S., Desprez T., Hocart C.H., Birch R.J., Baskin T.I., Burn J.E., Arioli T., Betzner A.S., Williamson R.E.
Plant Physiol. 126:278-288(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF SER-183; GLY-344 AND GLY-429.
[10]"Large-scale analysis of in vivo phosphorylated membrane proteins by immobilized metal ion affinity chromatography and mass spectrometry."
Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.
Mol. Cell. Proteomics 2:1234-1243(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: cv. La-0.
[11]"Phylogenetic analysis of the plant endo-beta-1,4-glucanase gene family."
Libertini E., Li Y., McQueen-Mason S.J.
J. Mol. Evol. 58:506-515(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY.
[12]"Phosphoproteomics of the Arabidopsis plasma membrane and a new phosphorylation site database."
Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.
Plant Cell 16:2394-2405(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]"The irregular xylem 2 mutant is an allele of korrigan that affects the secondary cell wall of Arabidopsis thaliana."
Szyjanowicz P.M., McKinnon I., Taylor N.G., Gardiner J., Jarvis M.C., Turner S.R.
Plant J. 37:730-740(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF PRO-250 AND PRO-553.
[14]"An Arabidopsis endo-1,4-beta-D-glucanase involved in cellulose synthesis undergoes regulated intracellular cycling."
Robert S., Bichet A., Grandjean O., Kierzkowski D., Satiat-Jeunemaitre B., Pelletier S., Hauser M.-T., Hoefte H., Vernhettes S.
Plant Cell 17:3378-3389(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[15]"Salt tolerance of Arabidopsis thaliana requires maturation of N-glycosylated proteins in the Golgi apparatus."
Kang J.S., Frank J., Kang C.H., Kajiura H., Vikram M., Ueda A., Kim S., Bahk J.D., Triplett B., Fujiyama K., Lee S.Y., von Schaewen A., Koiwa H.
Proc. Natl. Acad. Sci. U.S.A. 105:5933-5938(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U37702 mRNA. Translation: AAB60304.1.
AF073875 Genomic DNA. Translation: AAC83240.1.
AF074092 Genomic DNA. Translation: AAC33467.1.
AF074375 mRNA. Translation: AAC35344.1.
AB025613 Genomic DNA. Translation: BAA98160.1.
CP002688 Genomic DNA. Translation: AED95850.1.
AY037218 mRNA. Translation: AAK59818.1.
BT002221 mRNA. Translation: AAN72232.1.
AY086165 mRNA. Translation: AAM63370.1.
AK221941 mRNA. Translation: BAD94393.1.
AK222193 mRNA. Translation: BAD95336.1.
PIRS71215.
RefSeqNP_199783.1. NM_124350.2.
UniGeneAt.21098.
At.23416.

3D structure databases

ProteinModelPortalQ38890.
SMRQ38890. Positions 105-618.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid20281. 2 interactions.

Protein family/group databases

CAZyGH9. Glycoside Hydrolase Family 9.

Proteomic databases

PaxDbQ38890.
PRIDEQ38890.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G49720.1; AT5G49720.1; AT5G49720.
GeneID835035.
KEGGath:AT5G49720.

Organism-specific databases

TAIRAT5G49720.

Phylogenomic databases

eggNOGNOG257354.
HOGENOMHOG000021033.
InParanoidQ38890.
OMADRKIFMW.
PhylomeDBQ38890.
ProtClustDBCLSN2679649.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-2367.

Gene expression databases

GenevestigatorQ38890.

Family and domain databases

Gene3D1.50.10.10. 1 hit.
InterProIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
[Graphical view]
PfamPF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SUPFAMSSF48208. SSF48208. 1 hit.
PROSITEPS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGUN25_ARATH
AccessionPrimary (citable) accession number: Q38890
Secondary accession number(s): Q56W54 expand/collapse secondary AC list , Q56WU0, Q8H0S4, Q8LD74, Q94C24
Entry history
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names