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Q38890

- GUN25_ARATH

UniProt

Q38890 - GUN25_ARATH

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Protein

Endoglucanase 25

Gene

KOR

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Required for cellulose microfibrils formation. Involved in cell wall assembly during cell elongation and cell plate maturation in cytokinesis. Required for secondary cell wall formation in the developing xylem. May cycle through different intracellular compartments, including plasma membrane.5 Publications

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei513 – 5131By similarity
Active sitei561 – 5611By similarity
Active sitei570 – 5701By similarity

GO - Molecular functioni

  1. cellulase activity Source: TAIR

GO - Biological processi

  1. cellulose biosynthetic process Source: TAIR
  2. cellulose catabolic process Source: UniProtKB-KW
  3. cell wall organization Source: UniProtKB-KW
  4. cortical microtubule organization Source: TAIR
  5. hyperosmotic salinity response Source: TAIR
  6. response to cytokinin Source: TAIR
  7. shoot system development Source: TAIR
  8. unidimensional cell growth Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cell wall biogenesis/degradation, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-2367.

Protein family/group databases

CAZyiGH9. Glycoside Hydrolase Family 9.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase 25 (EC:3.2.1.4)
Alternative name(s):
Cellulase homolog OR16pep
Endo-1,4-beta glucanase 25
Protein KORRIGAN
Protein RADIALLY SWOLLEN 2
Gene namesi
Name:KOR
Synonyms:DEC, KOR1, RSW2
Ordered Locus Names:At5g49720
ORF Names:K2I5.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G49720.

Subcellular locationi

Cell membrane 1 Publication; Single-pass type II membrane protein 1 Publication
Note: Cell plate.

GO - Cellular componenti

  1. cell plate Source: TAIR
  2. early endosome Source: TAIR
  3. endosome Source: TAIR
  4. Golgi apparatus Source: TAIR
  5. integral component of membrane Source: UniProtKB-KW
  6. plasma membrane Source: TAIR
  7. trans-Golgi network Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Plants are extremely dwarf and show severe abnormal morphology with incomplete cell walls, aberrant cell plates and multinucleated cells.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi48 – 492LL → AA: Abolishes polarized targeting to cell plate. 1 Publication
Mutagenesisi59 – 591Y → A: Abolishes polarized targeting to cell plate. 1 Publication
Mutagenesisi183 – 1831S → N in rsw2-3; decrease in cellulose production. 1 Publication
Mutagenesisi250 – 2501P → L in irx2-1; decrease in cellulose production. 1 Publication
Mutagenesisi344 – 3441G → R in rsw2-4; decrease in cellulose production. 1 Publication
Mutagenesisi429 – 4291G → R in rsw2-1 and rsw2-2; decrease in cellulose production. 1 Publication
Mutagenesisi553 – 5531P → L in irx2-2; decrease in cellulose production. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 621621Endoglucanase 25PRO_0000249277Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi108 – 1081N-linked (GlcNAc...)Sequence Analysis
Glycosylationi133 – 1331N-linked (GlcNAc...)Sequence Analysis
Glycosylationi216 – 2161N-linked (GlcNAc...)Sequence Analysis
Glycosylationi324 – 3241N-linked (GlcNAc...)Sequence Analysis
Glycosylationi345 – 3451N-linked (GlcNAc...)Sequence Analysis
Glycosylationi408 – 4081N-linked (GlcNAc...)Sequence Analysis
Glycosylationi425 – 4251N-linked (GlcNAc...)Sequence Analysis
Glycosylationi567 – 5671N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Glycosylated. N-glycosylation of KOR in the endoplasmic reticulum followed by N-glycan modifications in the Golgi are essential for catalytic activity.1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ38890.
PRIDEiQ38890.

Expressioni

Tissue specificityi

Highly expressed in roots and stems, at intermediate levels in leaves and flowers, and at lower levels in siliques. Expressed in xylem (at protein level).3 Publications

Developmental stagei

Expressed during hypocotyl elongation in the dark.1 Publication

Gene expression databases

GenevestigatoriQ38890.

Interactioni

Protein-protein interaction databases

BioGridi20281. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ38890.
SMRiQ38890. Positions 105-618.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 7070CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini92 – 621530ExtracellularSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei71 – 9121Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni48 – 492Polarized targeting signal 1 (PTS1)
Regioni59 – 624Polarized targeting signal 2 (PTS2)

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG257354.
HOGENOMiHOG000021033.
InParanoidiQ38890.
OMAiDRKIFMW.
PhylomeDBiQ38890.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
[Graphical view]
PfamiPF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q38890 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MYGRDPWGGP LEINTADSAT DDDRSRNLND LDRAALSRPL DETQQSWLLG
60 70 80 90 100
PTEQKKKKYV DLGCIIVSRK IFVWTVGTLV AAALLAGFIT LIVKTVPRHH
110 120 130 140 150
PKTPPPDNYT IALHKALKFF NAQKSGKLPK HNNVSWRGNS GLQDGKGETG
160 170 180 190 200
SFYKDLVGGY YDAGDAIKFN FPMAYAMTML SWSVIEYSAK YEAAGELTHV
210 220 230 240 250
KELIKWGTDY FLKTFNSTAD SIDDLVSQVG SGNTDDGNTD PNDHYCWMRP
260 270 280 290 300
EDMDYKRPVT TCNGGCSDLA AEMAAALASA SIVFKDNKEY SKKLVHGAKV
310 320 330 340 350
VYQFGRTRRG RYSAGTAESS KFYNSSMYWD EFIWGGAWMY YATGNVTYLN
360 370 380 390 400
LITQPTMAKH AGAFWGGPYY GVFSWDNKLA GAQLLLSRLR LFLSPGYPYE
410 420 430 440 450
EILRTFHNQT SIVMCSYLPI FNKFNRTNGG LIELNHGAPQ PLQYSVNAAF
460 470 480 490 500
LATLYSDYLD AADTPGWYCG PNFYSTSVLR DFARSQIDYI LGKNPRKMSY
510 520 530 540 550
VVGFGTKYPR HVHHRGASIP KNKVKYNCKG GWKWRDSKKP NPNTIEGAMV
560 570 580 590 600
AGPDKRDGYR DVRMNYNYTE PTLAGNAGLV AALVALSGEE EATGKIDKNT
610 620
IFSAVPPLFP TPPPPPAPWK P
Length:621
Mass (Da):69,191
Last modified:November 1, 1996 - v1
Checksum:i798D8B8CC5DEBF42
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti7 – 71W → S in AAM63370. 1 PublicationCurated
Sequence conflicti59 – 591Y → D in AAK59818. (PubMed:14593172)Curated
Sequence conflicti338 – 3381W → R in AAM63370. 1 PublicationCurated
Sequence conflicti397 – 3971Y → C in AAK59818. (PubMed:14593172)Curated
Sequence conflicti397 – 3971Y → C in AAN72232. (PubMed:14593172)Curated
Sequence conflicti605 – 6051V → A in BAD94393. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U37702 mRNA. Translation: AAB60304.1.
AF073875 Genomic DNA. Translation: AAC83240.1.
AF074092 Genomic DNA. Translation: AAC33467.1.
AF074375 mRNA. Translation: AAC35344.1.
AB025613 Genomic DNA. Translation: BAA98160.1.
CP002688 Genomic DNA. Translation: AED95850.1.
AY037218 mRNA. Translation: AAK59818.1.
BT002221 mRNA. Translation: AAN72232.1.
AY086165 mRNA. Translation: AAM63370.1.
AK221941 mRNA. Translation: BAD94393.1.
AK222193 mRNA. Translation: BAD95336.1.
PIRiS71215.
RefSeqiNP_199783.1. NM_124350.2.
UniGeneiAt.21098.
At.23416.

Genome annotation databases

EnsemblPlantsiAT5G49720.1; AT5G49720.1; AT5G49720.
GeneIDi835035.
KEGGiath:AT5G49720.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U37702 mRNA. Translation: AAB60304.1 .
AF073875 Genomic DNA. Translation: AAC83240.1 .
AF074092 Genomic DNA. Translation: AAC33467.1 .
AF074375 mRNA. Translation: AAC35344.1 .
AB025613 Genomic DNA. Translation: BAA98160.1 .
CP002688 Genomic DNA. Translation: AED95850.1 .
AY037218 mRNA. Translation: AAK59818.1 .
BT002221 mRNA. Translation: AAN72232.1 .
AY086165 mRNA. Translation: AAM63370.1 .
AK221941 mRNA. Translation: BAD94393.1 .
AK222193 mRNA. Translation: BAD95336.1 .
PIRi S71215.
RefSeqi NP_199783.1. NM_124350.2.
UniGenei At.21098.
At.23416.

3D structure databases

ProteinModelPortali Q38890.
SMRi Q38890. Positions 105-618.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 20281. 2 interactions.

Protein family/group databases

CAZyi GH9. Glycoside Hydrolase Family 9.

Proteomic databases

PaxDbi Q38890.
PRIDEi Q38890.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G49720.1 ; AT5G49720.1 ; AT5G49720 .
GeneIDi 835035.
KEGGi ath:AT5G49720.

Organism-specific databases

TAIRi AT5G49720.

Phylogenomic databases

eggNOGi NOG257354.
HOGENOMi HOG000021033.
InParanoidi Q38890.
OMAi DRKIFMW.
PhylomeDBi Q38890.

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-2367.

Gene expression databases

Genevestigatori Q38890.

Family and domain databases

Gene3Di 1.50.10.10. 1 hit.
InterProi IPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
[Graphical view ]
Pfami PF00759. Glyco_hydro_9. 1 hit.
[Graphical view ]
SUPFAMi SSF48208. SSF48208. 1 hit.
PROSITEi PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "An Arabidopsis thaliana cDNA homologous to cellulase."
    Loebler M.
    DNA Seq. 8:253-256(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
    Tissue: Leaf.
  2. "A plasma membrane-bound putative endo-1,4-beta-D-glucanase is required for normal wall assembly and cell elongation in Arabidopsis."
    Nicol F., His I., Jauneau A., Vernhettes S., Canut H., Hoefte H.
    EMBO J. 17:5563-5576(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
    Strain: cv. Columbia.
  3. "KORRIGAN, an Arabidopsis endo-1,4-beta-glucanase, localizes to the cell plate by polarized targeting and is essential for cytokinesis."
    Zuo J., Niu Q.-W., Nishizawa N., Wu Y., Kost B., Chua N.-H.
    Plant Cell 12:1137-1152(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF 48-LYS-LYS-49 AND TYR-59.
    Strain: cv. Landsberg erecta.
  4. "Structural analysis of Arabidopsis thaliana chromosome 5. XI."
    Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H., Tabata S.
    Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  5. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  6. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  7. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  8. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 366-621.
    Strain: cv. Columbia.
  9. "Temperature-sensitive alleles of RSW2 link the KORRIGAN endo-1,4-beta-glucanase to cellulose synthesis and cytokinesis in Arabidopsis."
    Lane D.R., Wiedemeier A., Peng L., Hoefte H., Vernhettes S., Desprez T., Hocart C.H., Birch R.J., Baskin T.I., Burn J.E., Arioli T., Betzner A.S., Williamson R.E.
    Plant Physiol. 126:278-288(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF SER-183; GLY-344 AND GLY-429.
  10. "Large-scale analysis of in vivo phosphorylated membrane proteins by immobilized metal ion affinity chromatography and mass spectrometry."
    Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.
    Mol. Cell. Proteomics 2:1234-1243(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: cv. La-0.
  11. "Phylogenetic analysis of the plant endo-beta-1,4-glucanase gene family."
    Libertini E., Li Y., McQueen-Mason S.J.
    J. Mol. Evol. 58:506-515(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY.
  12. "Phosphoproteomics of the Arabidopsis plasma membrane and a new phosphorylation site database."
    Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.
    Plant Cell 16:2394-2405(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "The irregular xylem 2 mutant is an allele of korrigan that affects the secondary cell wall of Arabidopsis thaliana."
    Szyjanowicz P.M., McKinnon I., Taylor N.G., Gardiner J., Jarvis M.C., Turner S.R.
    Plant J. 37:730-740(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF PRO-250 AND PRO-553.
  14. "An Arabidopsis endo-1,4-beta-D-glucanase involved in cellulose synthesis undergoes regulated intracellular cycling."
    Robert S., Bichet A., Grandjean O., Kierzkowski D., Satiat-Jeunemaitre B., Pelletier S., Hauser M.-T., Hoefte H., Vernhettes S.
    Plant Cell 17:3378-3389(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  15. "Salt tolerance of Arabidopsis thaliana requires maturation of N-glycosylated proteins in the Golgi apparatus."
    Kang J.S., Frank J., Kang C.H., Kajiura H., Vikram M., Ueda A., Kim S., Bahk J.D., Triplett B., Fujiyama K., Lee S.Y., von Schaewen A., Koiwa H.
    Proc. Natl. Acad. Sci. U.S.A. 105:5933-5938(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION.

Entry informationi

Entry nameiGUN25_ARATH
AccessioniPrimary (citable) accession number: Q38890
Secondary accession number(s): Q56W54
, Q56WU0, Q8H0S4, Q8LD74, Q94C24
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3