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Protein

Endoglucanase 25

Gene

KOR

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for cellulose microfibrils formation. Involved in cell wall assembly during cell elongation and cell plate maturation in cytokinesis. Required for secondary cell wall formation in the developing xylem. May cycle through different intracellular compartments, including plasma membrane.5 Publications

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei513By similarity1
Active sitei561By similarity1
Active sitei570By similarity1

GO - Molecular functioni

  • cellulase activity Source: TAIR

GO - Biological processi

  • cellulose biosynthetic process Source: TAIR
  • cellulose catabolic process Source: UniProtKB-KW
  • cell wall organization Source: UniProtKB-KW
  • cortical microtubule organization Source: TAIR
  • hyperosmotic salinity response Source: TAIR
  • response to cytokinin Source: TAIR
  • shoot system development Source: TAIR
  • unidimensional cell growth Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cell wall biogenesis/degradation, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-2367.

Protein family/group databases

CAZyiGH9. Glycoside Hydrolase Family 9.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase 25 (EC:3.2.1.4)
Alternative name(s):
Cellulase homolog OR16pep
Endo-1,4-beta glucanase 25
Protein KORRIGAN
Protein RADIALLY SWOLLEN 2
Gene namesi
Name:KOR
Synonyms:DEC, KOR1, RSW2
Ordered Locus Names:At5g49720
ORF Names:K2I5.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G49720.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 70CytoplasmicSequence analysisAdd BLAST70
Transmembranei71 – 91Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini92 – 621ExtracellularSequence analysisAdd BLAST530

GO - Cellular componenti

  • cell plate Source: TAIR
  • early endosome Source: TAIR
  • endosome Source: TAIR
  • Golgi apparatus Source: TAIR
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: TAIR
  • trans-Golgi network Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Plants are extremely dwarf and show severe abnormal morphology with incomplete cell walls, aberrant cell plates and multinucleated cells.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi48 – 49LL → AA: Abolishes polarized targeting to cell plate. 1 Publication2
Mutagenesisi59Y → A: Abolishes polarized targeting to cell plate. 1 Publication1
Mutagenesisi183S → N in rsw2-3; decrease in cellulose production. 1 Publication1
Mutagenesisi250P → L in irx2-1; decrease in cellulose production. 1 Publication1
Mutagenesisi344G → R in rsw2-4; decrease in cellulose production. 1 Publication1
Mutagenesisi429G → R in rsw2-1 and rsw2-2; decrease in cellulose production. 1 Publication1
Mutagenesisi553P → L in irx2-2; decrease in cellulose production. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002492771 – 621Endoglucanase 25Add BLAST621

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi108N-linked (GlcNAc...)Sequence analysis1
Glycosylationi133N-linked (GlcNAc...)Sequence analysis1
Glycosylationi216N-linked (GlcNAc...)Sequence analysis1
Glycosylationi324N-linked (GlcNAc...)Sequence analysis1
Glycosylationi345N-linked (GlcNAc...)Sequence analysis1
Glycosylationi408N-linked (GlcNAc...)Sequence analysis1
Glycosylationi425N-linked (GlcNAc...)Sequence analysis1
Glycosylationi567N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Glycosylated. N-glycosylation of KOR in the endoplasmic reticulum followed by N-glycan modifications in the Golgi are essential for catalytic activity.1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ38890.

PTM databases

iPTMnetiQ38890.
SwissPalmiQ38890.

Expressioni

Tissue specificityi

Highly expressed in roots and stems, at intermediate levels in leaves and flowers, and at lower levels in siliques. Expressed in xylem (at protein level).3 Publications

Developmental stagei

Expressed during hypocotyl elongation in the dark.1 Publication

Gene expression databases

GenevisibleiQ38890. AT.

Interactioni

Protein-protein interaction databases

BioGridi20281. 8 interactors.
STRINGi3702.AT5G49720.1.

Structurei

3D structure databases

ProteinModelPortaliQ38890.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni48 – 49Polarized targeting signal 1 (PTS1)2
Regioni59 – 62Polarized targeting signal 2 (PTS2)4

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IE9Y. Eukaryota.
ENOG410XP0H. LUCA.
HOGENOMiHOG000021033.
InParanoidiQ38890.
OMAiEFVWGSS.
OrthoDBiEOG093604V7.
PhylomeDBiQ38890.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR001701. Glyco_hydro_9.
IPR033126. Glyco_hydro_9_Asp/Glu_AS.
IPR018221. Glyco_hydro_9_His_AS.
[Graphical view]
PfamiPF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q38890-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYGRDPWGGP LEINTADSAT DDDRSRNLND LDRAALSRPL DETQQSWLLG
60 70 80 90 100
PTEQKKKKYV DLGCIIVSRK IFVWTVGTLV AAALLAGFIT LIVKTVPRHH
110 120 130 140 150
PKTPPPDNYT IALHKALKFF NAQKSGKLPK HNNVSWRGNS GLQDGKGETG
160 170 180 190 200
SFYKDLVGGY YDAGDAIKFN FPMAYAMTML SWSVIEYSAK YEAAGELTHV
210 220 230 240 250
KELIKWGTDY FLKTFNSTAD SIDDLVSQVG SGNTDDGNTD PNDHYCWMRP
260 270 280 290 300
EDMDYKRPVT TCNGGCSDLA AEMAAALASA SIVFKDNKEY SKKLVHGAKV
310 320 330 340 350
VYQFGRTRRG RYSAGTAESS KFYNSSMYWD EFIWGGAWMY YATGNVTYLN
360 370 380 390 400
LITQPTMAKH AGAFWGGPYY GVFSWDNKLA GAQLLLSRLR LFLSPGYPYE
410 420 430 440 450
EILRTFHNQT SIVMCSYLPI FNKFNRTNGG LIELNHGAPQ PLQYSVNAAF
460 470 480 490 500
LATLYSDYLD AADTPGWYCG PNFYSTSVLR DFARSQIDYI LGKNPRKMSY
510 520 530 540 550
VVGFGTKYPR HVHHRGASIP KNKVKYNCKG GWKWRDSKKP NPNTIEGAMV
560 570 580 590 600
AGPDKRDGYR DVRMNYNYTE PTLAGNAGLV AALVALSGEE EATGKIDKNT
610 620
IFSAVPPLFP TPPPPPAPWK P
Length:621
Mass (Da):69,191
Last modified:November 1, 1996 - v1
Checksum:i798D8B8CC5DEBF42
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti7W → S in AAM63370 (Ref. 7) Curated1
Sequence conflicti59Y → D in AAK59818 (PubMed:14593172).Curated1
Sequence conflicti338W → R in AAM63370 (Ref. 7) Curated1
Sequence conflicti397Y → C in AAK59818 (PubMed:14593172).Curated1
Sequence conflicti397Y → C in AAN72232 (PubMed:14593172).Curated1
Sequence conflicti605V → A in BAD94393 (Ref. 8) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37702 mRNA. Translation: AAB60304.1.
AF073875 Genomic DNA. Translation: AAC83240.1.
AF074092 Genomic DNA. Translation: AAC33467.1.
AF074375 mRNA. Translation: AAC35344.1.
AB025613 Genomic DNA. Translation: BAA98160.1.
CP002688 Genomic DNA. Translation: AED95850.1.
AY037218 mRNA. Translation: AAK59818.1.
BT002221 mRNA. Translation: AAN72232.1.
AY086165 mRNA. Translation: AAM63370.1.
AK221941 mRNA. Translation: BAD94393.1.
AK222193 mRNA. Translation: BAD95336.1.
PIRiS71215.
RefSeqiNP_199783.1. NM_124350.3.
UniGeneiAt.21098.
At.23416.

Genome annotation databases

EnsemblPlantsiAT5G49720.1; AT5G49720.1; AT5G49720.
GeneIDi835035.
GrameneiAT5G49720.1; AT5G49720.1; AT5G49720.
KEGGiath:AT5G49720.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37702 mRNA. Translation: AAB60304.1.
AF073875 Genomic DNA. Translation: AAC83240.1.
AF074092 Genomic DNA. Translation: AAC33467.1.
AF074375 mRNA. Translation: AAC35344.1.
AB025613 Genomic DNA. Translation: BAA98160.1.
CP002688 Genomic DNA. Translation: AED95850.1.
AY037218 mRNA. Translation: AAK59818.1.
BT002221 mRNA. Translation: AAN72232.1.
AY086165 mRNA. Translation: AAM63370.1.
AK221941 mRNA. Translation: BAD94393.1.
AK222193 mRNA. Translation: BAD95336.1.
PIRiS71215.
RefSeqiNP_199783.1. NM_124350.3.
UniGeneiAt.21098.
At.23416.

3D structure databases

ProteinModelPortaliQ38890.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi20281. 8 interactors.
STRINGi3702.AT5G49720.1.

Protein family/group databases

CAZyiGH9. Glycoside Hydrolase Family 9.

PTM databases

iPTMnetiQ38890.
SwissPalmiQ38890.

Proteomic databases

PaxDbiQ38890.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G49720.1; AT5G49720.1; AT5G49720.
GeneIDi835035.
GrameneiAT5G49720.1; AT5G49720.1; AT5G49720.
KEGGiath:AT5G49720.

Organism-specific databases

TAIRiAT5G49720.

Phylogenomic databases

eggNOGiENOG410IE9Y. Eukaryota.
ENOG410XP0H. LUCA.
HOGENOMiHOG000021033.
InParanoidiQ38890.
OMAiEFVWGSS.
OrthoDBiEOG093604V7.
PhylomeDBiQ38890.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-2367.

Miscellaneous databases

PROiQ38890.

Gene expression databases

GenevisibleiQ38890. AT.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR001701. Glyco_hydro_9.
IPR033126. Glyco_hydro_9_Asp/Glu_AS.
IPR018221. Glyco_hydro_9_His_AS.
[Graphical view]
PfamiPF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGUN25_ARATH
AccessioniPrimary (citable) accession number: Q38890
Secondary accession number(s): Q56W54
, Q56WU0, Q8H0S4, Q8LD74, Q94C24
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.