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Q38890

- GUN25_ARATH

UniProt

Q38890 - GUN25_ARATH

Protein

Endoglucanase 25

Gene

KOR

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Required for cellulose microfibrils formation. Involved in cell wall assembly during cell elongation and cell plate maturation in cytokinesis. Required for secondary cell wall formation in the developing xylem. May cycle through different intracellular compartments, including plasma membrane.5 Publications

    Catalytic activityi

    Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei513 – 5131By similarity
    Active sitei561 – 5611By similarity
    Active sitei570 – 5701By similarity

    GO - Molecular functioni

    1. cellulase activity Source: TAIR

    GO - Biological processi

    1. cellulose biosynthetic process Source: TAIR
    2. cellulose catabolic process Source: UniProtKB-KW
    3. cortical microtubule organization Source: TAIR
    4. hyperosmotic salinity response Source: TAIR
    5. response to cytokinin Source: TAIR
    6. shoot system development Source: TAIR
    7. unidimensional cell growth Source: TAIR

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Cell wall biogenesis/degradation, Cellulose degradation, Polysaccharide degradation

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-2367.

    Protein family/group databases

    CAZyiGH9. Glycoside Hydrolase Family 9.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endoglucanase 25 (EC:3.2.1.4)
    Alternative name(s):
    Cellulase homolog OR16pep
    Endo-1,4-beta glucanase 25
    Protein KORRIGAN
    Protein RADIALLY SWOLLEN 2
    Gene namesi
    Name:KOR
    Synonyms:DEC, KOR1, RSW2
    Ordered Locus Names:At5g49720
    ORF Names:K2I5.8
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G49720.

    Subcellular locationi

    Cell membrane 1 Publication; Single-pass type II membrane protein 1 Publication
    Note: Cell plate.

    GO - Cellular componenti

    1. cell plate Source: TAIR
    2. early endosome Source: TAIR
    3. endosome Source: TAIR
    4. Golgi apparatus Source: TAIR
    5. integral component of membrane Source: UniProtKB-KW
    6. plasma membrane Source: TAIR
    7. trans-Golgi network Source: TAIR

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Plants are extremely dwarf and show severe abnormal morphology with incomplete cell walls, aberrant cell plates and multinucleated cells.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi48 – 492LL → AA: Abolishes polarized targeting to cell plate.
    Mutagenesisi59 – 591Y → A: Abolishes polarized targeting to cell plate. 1 Publication
    Mutagenesisi183 – 1831S → N in rsw2-3; decrease in cellulose production. 1 Publication
    Mutagenesisi250 – 2501P → L in irx2-1; decrease in cellulose production. 1 Publication
    Mutagenesisi344 – 3441G → R in rsw2-4; decrease in cellulose production. 1 Publication
    Mutagenesisi429 – 4291G → R in rsw2-1 and rsw2-2; decrease in cellulose production. 1 Publication
    Mutagenesisi553 – 5531P → L in irx2-2; decrease in cellulose production. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 621621Endoglucanase 25PRO_0000249277Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi108 – 1081N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi133 – 1331N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi216 – 2161N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi324 – 3241N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi345 – 3451N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi408 – 4081N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi425 – 4251N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi567 – 5671N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Glycosylated. N-glycosylation of KOR in the endoplasmic reticulum followed by N-glycan modifications in the Golgi are essential for catalytic activity.1 Publication

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ38890.
    PRIDEiQ38890.

    Expressioni

    Tissue specificityi

    Highly expressed in roots and stems, at intermediate levels in leaves and flowers, and at lower levels in siliques. Expressed in xylem (at protein level).3 Publications

    Developmental stagei

    Expressed during hypocotyl elongation in the dark.1 Publication

    Gene expression databases

    GenevestigatoriQ38890.

    Interactioni

    Protein-protein interaction databases

    BioGridi20281. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ38890.
    SMRiQ38890. Positions 105-618.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 7070CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini92 – 621530ExtracellularSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei71 – 9121Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni48 – 492Polarized targeting signal 1 (PTS1)
    Regioni59 – 624Polarized targeting signal 2 (PTS2)

    Sequence similaritiesi

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG257354.
    HOGENOMiHOG000021033.
    InParanoidiQ38890.
    OMAiDRKIFMW.
    PhylomeDBiQ38890.

    Family and domain databases

    Gene3Di1.50.10.10. 1 hit.
    InterProiIPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR001701. Glyco_hydro_9.
    IPR018221. Glyco_hydro_9_AS.
    [Graphical view]
    PfamiPF00759. Glyco_hydro_9. 1 hit.
    [Graphical view]
    SUPFAMiSSF48208. SSF48208. 1 hit.
    PROSITEiPS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
    PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q38890-1 [UniParc]FASTAAdd to Basket

    « Hide

    MYGRDPWGGP LEINTADSAT DDDRSRNLND LDRAALSRPL DETQQSWLLG    50
    PTEQKKKKYV DLGCIIVSRK IFVWTVGTLV AAALLAGFIT LIVKTVPRHH 100
    PKTPPPDNYT IALHKALKFF NAQKSGKLPK HNNVSWRGNS GLQDGKGETG 150
    SFYKDLVGGY YDAGDAIKFN FPMAYAMTML SWSVIEYSAK YEAAGELTHV 200
    KELIKWGTDY FLKTFNSTAD SIDDLVSQVG SGNTDDGNTD PNDHYCWMRP 250
    EDMDYKRPVT TCNGGCSDLA AEMAAALASA SIVFKDNKEY SKKLVHGAKV 300
    VYQFGRTRRG RYSAGTAESS KFYNSSMYWD EFIWGGAWMY YATGNVTYLN 350
    LITQPTMAKH AGAFWGGPYY GVFSWDNKLA GAQLLLSRLR LFLSPGYPYE 400
    EILRTFHNQT SIVMCSYLPI FNKFNRTNGG LIELNHGAPQ PLQYSVNAAF 450
    LATLYSDYLD AADTPGWYCG PNFYSTSVLR DFARSQIDYI LGKNPRKMSY 500
    VVGFGTKYPR HVHHRGASIP KNKVKYNCKG GWKWRDSKKP NPNTIEGAMV 550
    AGPDKRDGYR DVRMNYNYTE PTLAGNAGLV AALVALSGEE EATGKIDKNT 600
    IFSAVPPLFP TPPPPPAPWK P 621
    Length:621
    Mass (Da):69,191
    Last modified:November 1, 1996 - v1
    Checksum:i798D8B8CC5DEBF42
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti7 – 71W → S in AAM63370. 1 PublicationCurated
    Sequence conflicti59 – 591Y → D in AAK59818. (PubMed:14593172)Curated
    Sequence conflicti338 – 3381W → R in AAM63370. 1 PublicationCurated
    Sequence conflicti397 – 3971Y → C in AAK59818. (PubMed:14593172)Curated
    Sequence conflicti397 – 3971Y → C in AAN72232. (PubMed:14593172)Curated
    Sequence conflicti605 – 6051V → A in BAD94393. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U37702 mRNA. Translation: AAB60304.1.
    AF073875 Genomic DNA. Translation: AAC83240.1.
    AF074092 Genomic DNA. Translation: AAC33467.1.
    AF074375 mRNA. Translation: AAC35344.1.
    AB025613 Genomic DNA. Translation: BAA98160.1.
    CP002688 Genomic DNA. Translation: AED95850.1.
    AY037218 mRNA. Translation: AAK59818.1.
    BT002221 mRNA. Translation: AAN72232.1.
    AY086165 mRNA. Translation: AAM63370.1.
    AK221941 mRNA. Translation: BAD94393.1.
    AK222193 mRNA. Translation: BAD95336.1.
    PIRiS71215.
    RefSeqiNP_199783.1. NM_124350.2.
    UniGeneiAt.21098.
    At.23416.

    Genome annotation databases

    EnsemblPlantsiAT5G49720.1; AT5G49720.1; AT5G49720.
    GeneIDi835035.
    KEGGiath:AT5G49720.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U37702 mRNA. Translation: AAB60304.1 .
    AF073875 Genomic DNA. Translation: AAC83240.1 .
    AF074092 Genomic DNA. Translation: AAC33467.1 .
    AF074375 mRNA. Translation: AAC35344.1 .
    AB025613 Genomic DNA. Translation: BAA98160.1 .
    CP002688 Genomic DNA. Translation: AED95850.1 .
    AY037218 mRNA. Translation: AAK59818.1 .
    BT002221 mRNA. Translation: AAN72232.1 .
    AY086165 mRNA. Translation: AAM63370.1 .
    AK221941 mRNA. Translation: BAD94393.1 .
    AK222193 mRNA. Translation: BAD95336.1 .
    PIRi S71215.
    RefSeqi NP_199783.1. NM_124350.2.
    UniGenei At.21098.
    At.23416.

    3D structure databases

    ProteinModelPortali Q38890.
    SMRi Q38890. Positions 105-618.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 20281. 2 interactions.

    Protein family/group databases

    CAZyi GH9. Glycoside Hydrolase Family 9.

    Proteomic databases

    PaxDbi Q38890.
    PRIDEi Q38890.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G49720.1 ; AT5G49720.1 ; AT5G49720 .
    GeneIDi 835035.
    KEGGi ath:AT5G49720.

    Organism-specific databases

    TAIRi AT5G49720.

    Phylogenomic databases

    eggNOGi NOG257354.
    HOGENOMi HOG000021033.
    InParanoidi Q38890.
    OMAi DRKIFMW.
    PhylomeDBi Q38890.

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-2367.

    Gene expression databases

    Genevestigatori Q38890.

    Family and domain databases

    Gene3Di 1.50.10.10. 1 hit.
    InterProi IPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR001701. Glyco_hydro_9.
    IPR018221. Glyco_hydro_9_AS.
    [Graphical view ]
    Pfami PF00759. Glyco_hydro_9. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48208. SSF48208. 1 hit.
    PROSITEi PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
    PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "An Arabidopsis thaliana cDNA homologous to cellulase."
      Loebler M.
      DNA Seq. 8:253-256(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Columbia.
      Tissue: Leaf.
    2. "A plasma membrane-bound putative endo-1,4-beta-D-glucanase is required for normal wall assembly and cell elongation in Arabidopsis."
      Nicol F., His I., Jauneau A., Vernhettes S., Canut H., Hoefte H.
      EMBO J. 17:5563-5576(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
      Strain: cv. Columbia.
    3. "KORRIGAN, an Arabidopsis endo-1,4-beta-glucanase, localizes to the cell plate by polarized targeting and is essential for cytokinesis."
      Zuo J., Niu Q.-W., Nishizawa N., Wu Y., Kost B., Chua N.-H.
      Plant Cell 12:1137-1152(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF 48-LYS-LYS-49 AND TYR-59.
      Strain: cv. Landsberg erecta.
    4. "Structural analysis of Arabidopsis thaliana chromosome 5. XI."
      Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H., Tabata S.
      Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    5. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    6. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    7. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    8. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 366-621.
      Strain: cv. Columbia.
    9. "Temperature-sensitive alleles of RSW2 link the KORRIGAN endo-1,4-beta-glucanase to cellulose synthesis and cytokinesis in Arabidopsis."
      Lane D.R., Wiedemeier A., Peng L., Hoefte H., Vernhettes S., Desprez T., Hocart C.H., Birch R.J., Baskin T.I., Burn J.E., Arioli T., Betzner A.S., Williamson R.E.
      Plant Physiol. 126:278-288(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF SER-183; GLY-344 AND GLY-429.
    10. "Large-scale analysis of in vivo phosphorylated membrane proteins by immobilized metal ion affinity chromatography and mass spectrometry."
      Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.
      Mol. Cell. Proteomics 2:1234-1243(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: cv. La-0.
    11. "Phylogenetic analysis of the plant endo-beta-1,4-glucanase gene family."
      Libertini E., Li Y., McQueen-Mason S.J.
      J. Mol. Evol. 58:506-515(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY.
    12. "Phosphoproteomics of the Arabidopsis plasma membrane and a new phosphorylation site database."
      Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.
      Plant Cell 16:2394-2405(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. "The irregular xylem 2 mutant is an allele of korrigan that affects the secondary cell wall of Arabidopsis thaliana."
      Szyjanowicz P.M., McKinnon I., Taylor N.G., Gardiner J., Jarvis M.C., Turner S.R.
      Plant J. 37:730-740(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF PRO-250 AND PRO-553.
    14. "An Arabidopsis endo-1,4-beta-D-glucanase involved in cellulose synthesis undergoes regulated intracellular cycling."
      Robert S., Bichet A., Grandjean O., Kierzkowski D., Satiat-Jeunemaitre B., Pelletier S., Hauser M.-T., Hoefte H., Vernhettes S.
      Plant Cell 17:3378-3389(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    15. "Salt tolerance of Arabidopsis thaliana requires maturation of N-glycosylated proteins in the Golgi apparatus."
      Kang J.S., Frank J., Kang C.H., Kajiura H., Vikram M., Ueda A., Kim S., Bahk J.D., Triplett B., Fujiyama K., Lee S.Y., von Schaewen A., Koiwa H.
      Proc. Natl. Acad. Sci. U.S.A. 105:5933-5938(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION.

    Entry informationi

    Entry nameiGUN25_ARATH
    AccessioniPrimary (citable) accession number: Q38890
    Secondary accession number(s): Q56W54
    , Q56WU0, Q8H0S4, Q8LD74, Q94C24
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 5, 2006
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3