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Protein

Preprotein translocase subunit SCY1, chloroplastic

Gene

SCY1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in protein export. Probably interacts with other proteins to allow the translocation of proteins across the chloroplast thylakoid membranes. Required for normal greening during embryogenesis. Central subunit of the protein translocation channel SecYE. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug (By similarity).By similarity1 Publication

GO - Biological processi

  • protein transport Source: UniProtKB-KW
  • thylakoid membrane organization Source: TAIR
Complete GO annotation...

Keywords - Biological processi

Protein transport, Translocation, Transport

Protein family/group databases

TCDBi3.A.5.4.2. the general secretory pathway (sec) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Preprotein translocase subunit SCY1, chloroplastic
Alternative name(s):
CpSecY
Gene namesi
Name:SCY1
Synonyms:SECY
Ordered Locus Names:At2g18710
ORF Names:MSF3.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G18710.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei142 – 16221HelicalSequence analysisAdd
BLAST
Transmembranei192 – 21221HelicalSequence analysisAdd
BLAST
Transmembranei241 – 26121HelicalSequence analysisAdd
BLAST
Transmembranei268 – 28821HelicalSequence analysisAdd
BLAST
Transmembranei295 – 31521HelicalSequence analysisAdd
BLAST
Transmembranei328 – 34821HelicalSequence analysisAdd
BLAST
Transmembranei382 – 40221HelicalSequence analysisAdd
BLAST
Transmembranei415 – 43521HelicalSequence analysisAdd
BLAST
Transmembranei482 – 50221HelicalSequence analysisAdd
BLAST
Transmembranei503 – 52321HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast thylakoid membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Thylakoid

Pathology & Biotechi

Disruption phenotypei

Seedling lethal. Albino seedlings with yellow and translucent (glassy) lateral organs when grown heterotrophically.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 6767ChloroplastSequence analysisAdd
BLAST
Chaini68 – 551484Preprotein translocase subunit SCY1, chloroplasticPRO_0000031995Add
BLAST

Proteomic databases

PaxDbiQ38885.
PRIDEiQ38885.

Expressioni

Gene expression databases

GenevisibleiQ38885. AT.

Interactioni

Subunit structurei

Part of the Sec protein translocation apparatus. Interacts with SECE1, ALB3 and probably with SECA1.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ALB3Q8LBP48EBI-1806802,EBI-1806831
SECE1O233423EBI-1806802,EBI-1806811

Protein-protein interaction databases

BioGridi1743. 2 interactions.
IntActiQ38885. 4 interactions.
STRINGi3702.AT2G18710.1.

Structurei

3D structure databases

ProteinModelPortaliQ38885.
SMRiQ38885. Positions 141-540.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SecY/SEC61-alpha family.Curated

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IHRV. Eukaryota.
COG0201. LUCA.
HOGENOMiHOG000080585.
InParanoidiQ38885.
KOiK10956.
OMAiQTYVISQ.
OrthoDBiEOG093609ZU.
PhylomeDBiQ38885.

Family and domain databases

Gene3Di1.10.3370.10. 1 hit.
HAMAPiMF_01465. SecY. 1 hit.
InterProiIPR026593. SecY.
IPR002208. SecY/SEC61-alpha.
IPR030659. SecY_CS.
IPR023201. SecY_su_dom.
[Graphical view]
PANTHERiPTHR10906. PTHR10906. 1 hit.
PfamiPF00344. SecY. 1 hit.
[Graphical view]
SUPFAMiSSF103491. SSF103491. 1 hit.
TIGRFAMsiTIGR00967. 3a0501s007. 1 hit.
PROSITEiPS00755. SECY_1. 1 hit.
PS00756. SECY_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q38885-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MITVSEVSSY SSSSSNFASL SRLNHKSSSR LRSSSLYKGS FFSVSTKTRR
60 70 80 90 100
NTCKAKSWNL GLVINSRSSE ASVFDPLGIN PDETSGLSSI WESFVSLLSP
110 120 130 140 150
SFESSSGNRR DKPSSGRGVA AAIEDSSIDF GDFFKGPLPG KFLKLLGFLA
160 170 180 190 200
LSRLGIYIPL GGVNREAFVG NLDQNSILST LDTFSGGGIG RLGICSLGIV
210 220 230 240 250
PFINAQIVFQ LLAQVYPKLQ DLQKKEGEAG RKKILQYTRY ASVGFAIVQA
260 270 280 290 300
IGQVFYLRPY VNDFSTEWVV SSVTLLTLGS VLTTYIGERI SDLKLGNGTS
310 320 330 340 350
LLIFTSIISY LPASFGRTTA EALQEGNYTG LGTIVVSFLL LVLGIVYVQE
360 370 380 390 400
AERKIPLNYA SRYTSKAGGL QKSAYLPFKV NSAGVMPIIF STSSLALPAT
410 420 430 440 450
LARFTGISAL KNVAFALTPG GSFYLPTNIL LIAFFNYYYT FLQLDPDDVS
460 470 480 490 500
EQLKRQGASI PLVRPGKSTA LFIKTVLGRI SVLGSAFLAV LAAGPAVVEQ
510 520 530 540 550
ITHLTAFRGF AGTSVLILVG CATDTARKVQ AEIISQKYKN IEFYELDKYD

P
Length:551
Mass (Da):59,492
Last modified:January 11, 2001 - v2
Checksum:i526854797A3B8059
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti281 – 2811V → L in AAB60305 (PubMed:7629062).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37247 mRNA. Translation: AAB60305.1.
AC005724 Genomic DNA. Translation: AAD08940.1.
CP002685 Genomic DNA. Translation: AEC06796.1.
AF424550 mRNA. Translation: AAL11544.1.
AY058103 mRNA. Translation: AAL24211.1.
AY065093 mRNA. Translation: AAL38269.1.
BT002632 mRNA. Translation: AAO11548.1.
PIRiA57189.
F84567.
RefSeqiNP_179461.1. NM_127427.3.
UniGeneiAt.10218.

Genome annotation databases

EnsemblPlantsiAT2G18710.1; AT2G18710.1; AT2G18710.
GeneIDi816386.
GrameneiAT2G18710.1; AT2G18710.1; AT2G18710.
KEGGiath:AT2G18710.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37247 mRNA. Translation: AAB60305.1.
AC005724 Genomic DNA. Translation: AAD08940.1.
CP002685 Genomic DNA. Translation: AEC06796.1.
AF424550 mRNA. Translation: AAL11544.1.
AY058103 mRNA. Translation: AAL24211.1.
AY065093 mRNA. Translation: AAL38269.1.
BT002632 mRNA. Translation: AAO11548.1.
PIRiA57189.
F84567.
RefSeqiNP_179461.1. NM_127427.3.
UniGeneiAt.10218.

3D structure databases

ProteinModelPortaliQ38885.
SMRiQ38885. Positions 141-540.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi1743. 2 interactions.
IntActiQ38885. 4 interactions.
STRINGi3702.AT2G18710.1.

Protein family/group databases

TCDBi3.A.5.4.2. the general secretory pathway (sec) family.

Proteomic databases

PaxDbiQ38885.
PRIDEiQ38885.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G18710.1; AT2G18710.1; AT2G18710.
GeneIDi816386.
GrameneiAT2G18710.1; AT2G18710.1; AT2G18710.
KEGGiath:AT2G18710.

Organism-specific databases

TAIRiAT2G18710.

Phylogenomic databases

eggNOGiENOG410IHRV. Eukaryota.
COG0201. LUCA.
HOGENOMiHOG000080585.
InParanoidiQ38885.
KOiK10956.
OMAiQTYVISQ.
OrthoDBiEOG093609ZU.
PhylomeDBiQ38885.

Miscellaneous databases

PROiQ38885.

Gene expression databases

GenevisibleiQ38885. AT.

Family and domain databases

Gene3Di1.10.3370.10. 1 hit.
HAMAPiMF_01465. SecY. 1 hit.
InterProiIPR026593. SecY.
IPR002208. SecY/SEC61-alpha.
IPR030659. SecY_CS.
IPR023201. SecY_su_dom.
[Graphical view]
PANTHERiPTHR10906. PTHR10906. 1 hit.
PfamiPF00344. SecY. 1 hit.
[Graphical view]
SUPFAMiSSF103491. SSF103491. 1 hit.
TIGRFAMsiTIGR00967. 3a0501s007. 1 hit.
PROSITEiPS00755. SECY_1. 1 hit.
PS00756. SECY_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSCY1_ARATH
AccessioniPrimary (citable) accession number: Q38885
Secondary accession number(s): Q9SLG1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 11, 2001
Last modified: September 7, 2016
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Cannot substitute for SCY2.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.