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Protein

Preprotein translocase subunit SCY1, chloroplastic

Gene

SCY1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in protein export. Probably interacts with other proteins to allow the translocation of proteins across the chloroplast thylakoid membranes. Required for normal greening during embryogenesis. Central subunit of the protein translocation channel SecYE. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug (By similarity).By similarity1 Publication

Miscellaneous

Cannot substitute for SCY2.

GO - Biological processi

  • protein transport Source: UniProtKB-KW
  • thylakoid membrane organization Source: TAIR

Keywordsi

Biological processProtein transport, Translocation, Transport

Protein family/group databases

TCDBi3.A.5.4.2 the general secretory pathway (sec) family

Names & Taxonomyi

Protein namesi
Recommended name:
Preprotein translocase subunit SCY1, chloroplastic
Alternative name(s):
CpSecY
Gene namesi
Name:SCY1
Synonyms:SECY
Ordered Locus Names:At2g18710
ORF Names:MSF3.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G18710
TAIRilocus:2054038 AT2G18710

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei142 – 162HelicalSequence analysisAdd BLAST21
Transmembranei192 – 212HelicalSequence analysisAdd BLAST21
Transmembranei241 – 261HelicalSequence analysisAdd BLAST21
Transmembranei268 – 288HelicalSequence analysisAdd BLAST21
Transmembranei295 – 315HelicalSequence analysisAdd BLAST21
Transmembranei328 – 348HelicalSequence analysisAdd BLAST21
Transmembranei382 – 402HelicalSequence analysisAdd BLAST21
Transmembranei415 – 435HelicalSequence analysisAdd BLAST21
Transmembranei482 – 502HelicalSequence analysisAdd BLAST21
Transmembranei503 – 523HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Thylakoid

Pathology & Biotechi

Disruption phenotypei

Seedling lethal. Albino seedlings with yellow and translucent (glassy) lateral organs when grown heterotrophically.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 67ChloroplastSequence analysisAdd BLAST67
ChainiPRO_000003199568 – 551Preprotein translocase subunit SCY1, chloroplasticAdd BLAST484

Proteomic databases

PaxDbiQ38885

Expressioni

Gene expression databases

ExpressionAtlasiQ38885 baseline and differential
GenevisibleiQ38885 AT

Interactioni

Subunit structurei

Part of the Sec protein translocation apparatus. Interacts with SECE1, ALB3 and probably with SECA1.3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi1743, 2 interactors
IntActiQ38885, 4 interactors
STRINGi3702.AT2G18710.1

Structurei

3D structure databases

ProteinModelPortaliQ38885
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SecY/SEC61-alpha family.Curated

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IHRV Eukaryota
COG0201 LUCA
HOGENOMiHOG000080585
InParanoidiQ38885
KOiK10956
OMAiQTYVISQ
OrthoDBiEOG093609ZU
PhylomeDBiQ38885

Family and domain databases

Gene3Di1.10.3370.10, 1 hit
HAMAPiMF_01465 SecY, 1 hit
InterProiView protein in InterPro
IPR026593 SecY
IPR002208 SecY/SEC61-alpha
IPR030659 SecY_CS
IPR023201 SecY_dom_sf
PANTHERiPTHR10906 PTHR10906, 1 hit
PfamiView protein in Pfam
PF00344 SecY, 1 hit
SUPFAMiSSF103491 SSF103491, 1 hit
TIGRFAMsiTIGR00967 3a0501s007, 1 hit
PROSITEiView protein in PROSITE
PS00755 SECY_1, 1 hit
PS00756 SECY_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q38885-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MITVSEVSSY SSSSSNFASL SRLNHKSSSR LRSSSLYKGS FFSVSTKTRR
60 70 80 90 100
NTCKAKSWNL GLVINSRSSE ASVFDPLGIN PDETSGLSSI WESFVSLLSP
110 120 130 140 150
SFESSSGNRR DKPSSGRGVA AAIEDSSIDF GDFFKGPLPG KFLKLLGFLA
160 170 180 190 200
LSRLGIYIPL GGVNREAFVG NLDQNSILST LDTFSGGGIG RLGICSLGIV
210 220 230 240 250
PFINAQIVFQ LLAQVYPKLQ DLQKKEGEAG RKKILQYTRY ASVGFAIVQA
260 270 280 290 300
IGQVFYLRPY VNDFSTEWVV SSVTLLTLGS VLTTYIGERI SDLKLGNGTS
310 320 330 340 350
LLIFTSIISY LPASFGRTTA EALQEGNYTG LGTIVVSFLL LVLGIVYVQE
360 370 380 390 400
AERKIPLNYA SRYTSKAGGL QKSAYLPFKV NSAGVMPIIF STSSLALPAT
410 420 430 440 450
LARFTGISAL KNVAFALTPG GSFYLPTNIL LIAFFNYYYT FLQLDPDDVS
460 470 480 490 500
EQLKRQGASI PLVRPGKSTA LFIKTVLGRI SVLGSAFLAV LAAGPAVVEQ
510 520 530 540 550
ITHLTAFRGF AGTSVLILVG CATDTARKVQ AEIISQKYKN IEFYELDKYD

P
Length:551
Mass (Da):59,492
Last modified:January 11, 2001 - v2
Checksum:i526854797A3B8059
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti281V → L in AAB60305 (PubMed:7629062).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37247 mRNA Translation: AAB60305.1
AC005724 Genomic DNA Translation: AAD08940.1
CP002685 Genomic DNA Translation: AEC06796.1
AF424550 mRNA Translation: AAL11544.1
AY058103 mRNA Translation: AAL24211.1
AY065093 mRNA Translation: AAL38269.1
BT002632 mRNA Translation: AAO11548.1
PIRiA57189
F84567
RefSeqiNP_179461.1, NM_127427.3
UniGeneiAt.10218

Genome annotation databases

EnsemblPlantsiAT2G18710.1; AT2G18710.1; AT2G18710
GeneIDi816386
GrameneiAT2G18710.1; AT2G18710.1; AT2G18710
KEGGiath:AT2G18710

Similar proteinsi

Entry informationi

Entry nameiSCY1_ARATH
AccessioniPrimary (citable) accession number: Q38885
Secondary accession number(s): Q9SLG1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 11, 2001
Last modified: April 25, 2018
This is version 124 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health