Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Eukaryotic translation initiation factor 3 subunit I

Gene

TIF3I1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.UniRule annotation

GO - Molecular functioni

GO - Biological processi

  • formation of translation preinitiation complex Source: UniProtKB-HAMAP
  • regulation of translational initiation Source: UniProtKB-HAMAP
  • response to cadmium ion Source: TAIR
  • response to salt stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis

Enzyme and pathway databases

ReactomeiR-ATH-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-ATH-72689. Formation of a pool of free 40S subunits.
R-ATH-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-ATH-72702. Ribosomal scanning and start codon recognition.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 3 subunit IUniRule annotation
Short name:
eIF3iUniRule annotation
Alternative name(s):
Eukaryotic translation initiation factor 3 subunit 2UniRule annotation
TGF-beta receptor-interacting protein 1
Short name:
TRIP-1
eIF-3-betaUniRule annotation
eIF3 p36UniRule annotation
Gene namesi
Name:TIF3I1
Ordered Locus Names:At2g46280
ORF Names:T3F17.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G46280.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000510391 – 328Eukaryotic translation initiation factor 3 subunit IAdd BLAST328

Proteomic databases

PaxDbiQ38884.
PRIDEiQ38884.

PTM databases

iPTMnetiQ38884.

Expressioni

Gene expression databases

ExpressionAtlasiQ38884. baseline and differential.
GenevisibleiQ38884. AT.

Interactioni

Subunit structurei

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.UniRule annotation

Protein-protein interaction databases

BioGridi4571. 5 interactors.
IntActiQ38884. 2 interactors.
STRINGi3702.AT2G46280.1.

Structurei

3D structure databases

ProteinModelPortaliQ38884.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati8 – 49WD 1Add BLAST42
Repeati50 – 89WD 2Add BLAST40
Repeati146 – 185WD 3Add BLAST40
Repeati191 – 230WD 4Add BLAST40
Repeati288 – 327WD 5Add BLAST40

Sequence similaritiesi

Belongs to the eIF-3 subunit I family.UniRule annotation
Contains 5 WD repeats.UniRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0643. Eukaryota.
ENOG410XQ3E. LUCA.
HOGENOMiHOG000231322.
InParanoidiQ38884.
KOiK03246.
OMAiPYLSVPC.
OrthoDBiEOG09360FYR.
PhylomeDBiQ38884.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
HAMAPiMF_03008. eIF3i. 1 hit.
InterProiIPR027525. eIF3i.
IPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 4 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q38884-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRPILMKGHE RPLTFLRYNR EGDLLFSCAK DHTPTLWFAD NGERLGTYRG
60 70 80 90 100
HNGAVWCCDV SRDSSRLITG SADQTAKLWD VKSGKELFTF KFNAPTRSVD
110 120 130 140 150
FAVGDRLAVI TTDHFVDRTA AIHVKRIAED PEEQDAESVL VLHCPDGKKR
160 170 180 190 200
INRAVWGPLN QTIVSGGEDK VIRIWDAETG KLLKQSDEEV GHKKDITSLC
210 220 230 240 250
KAADDSHFLT GSLDKTAKLW DMRTLTLLKT YTTVVPVNAV SLSPLLNHVV
260 270 280 290 300
LGGGQDASAV TTTDHRAGKF EAKFYDKILQ EEIGGVKGHF GPINALAFNP
310 320
DGKSFSSGGE DGYVRLHHFD SDYFNIKI
Length:328
Mass (Da):36,388
Last modified:September 19, 2002 - v2
Checksum:i4ABFE9866A09CFAF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti21 – 23EGD → QPH in AAG53616 (PubMed:11042177).Curated3
Sequence conflicti107L → F in AAG53616 (PubMed:11042177).Curated1
Sequence conflicti126 – 128RIA → AYC in AAC49079 (PubMed:7566156).Curated3
Sequence conflicti166G → C in AAC49079 (PubMed:7566156).Curated1
Sequence conflicti255Q → E in AAC49079 (PubMed:7566156).Curated1
Sequence conflicti275Y → C in AAC49079 (PubMed:7566156).Curated1
Sequence conflicti304S → G in AAK43862 (PubMed:14593172).Curated1
Sequence conflicti304S → G in AAL47346 (PubMed:14593172).Curated1
Sequence conflicti311D → G in AAC49079 (PubMed:7566156).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U36765 mRNA. Translation: AAC49079.1.
AF285835 mRNA. Translation: AAG53616.1.
AC005397 Genomic DNA. Translation: AAC62878.1.
CP002685 Genomic DNA. Translation: AEC10670.1.
CP002685 Genomic DNA. Translation: AEC10671.1.
AF370485 mRNA. Translation: AAK43862.1.
AY064633 mRNA. Translation: AAL47346.1.
PIRiH84900.
S60256.
RefSeqiNP_182151.1. NM_130191.6. [Q38884-1]
NP_850450.1. NM_180119.3. [Q38884-1]
UniGeneiAt.21646.

Genome annotation databases

EnsemblPlantsiAT2G46280.1; AT2G46280.1; AT2G46280. [Q38884-1]
AT2G46280.2; AT2G46280.2; AT2G46280. [Q38884-1]
GeneIDi819236.
GrameneiAT2G46280.1; AT2G46280.1; AT2G46280.
AT2G46280.2; AT2G46280.2; AT2G46280.
KEGGiath:AT2G46280.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U36765 mRNA. Translation: AAC49079.1.
AF285835 mRNA. Translation: AAG53616.1.
AC005397 Genomic DNA. Translation: AAC62878.1.
CP002685 Genomic DNA. Translation: AEC10670.1.
CP002685 Genomic DNA. Translation: AEC10671.1.
AF370485 mRNA. Translation: AAK43862.1.
AY064633 mRNA. Translation: AAL47346.1.
PIRiH84900.
S60256.
RefSeqiNP_182151.1. NM_130191.6. [Q38884-1]
NP_850450.1. NM_180119.3. [Q38884-1]
UniGeneiAt.21646.

3D structure databases

ProteinModelPortaliQ38884.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4571. 5 interactors.
IntActiQ38884. 2 interactors.
STRINGi3702.AT2G46280.1.

PTM databases

iPTMnetiQ38884.

Proteomic databases

PaxDbiQ38884.
PRIDEiQ38884.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G46280.1; AT2G46280.1; AT2G46280. [Q38884-1]
AT2G46280.2; AT2G46280.2; AT2G46280. [Q38884-1]
GeneIDi819236.
GrameneiAT2G46280.1; AT2G46280.1; AT2G46280.
AT2G46280.2; AT2G46280.2; AT2G46280.
KEGGiath:AT2G46280.

Organism-specific databases

TAIRiAT2G46280.

Phylogenomic databases

eggNOGiKOG0643. Eukaryota.
ENOG410XQ3E. LUCA.
HOGENOMiHOG000231322.
InParanoidiQ38884.
KOiK03246.
OMAiPYLSVPC.
OrthoDBiEOG09360FYR.
PhylomeDBiQ38884.

Enzyme and pathway databases

ReactomeiR-ATH-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-ATH-72689. Formation of a pool of free 40S subunits.
R-ATH-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-ATH-72702. Ribosomal scanning and start codon recognition.

Miscellaneous databases

PROiQ38884.

Gene expression databases

ExpressionAtlasiQ38884. baseline and differential.
GenevisibleiQ38884. AT.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
HAMAPiMF_03008. eIF3i. 1 hit.
InterProiIPR027525. eIF3i.
IPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 4 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEIF3I_ARATH
AccessioniPrimary (citable) accession number: Q38884
Secondary accession number(s): O82342, Q94K09, Q9C5Z0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: September 19, 2002
Last modified: November 30, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Translation initiation factors
    List of translation initiation factor entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.