Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Calcium-dependent protein kinase 2

Gene

CPK2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in signal transduction pathways that involve calcium as a second messenger.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by calcium. Autophosphorylation may play an important role in the regulation of the kinase activity (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei215ATPPROSITE-ProRule annotation1
Active sitei310Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi192 – 200ATPPROSITE-ProRule annotation9
Calcium bindingi500 – 5111PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi536 – 5472PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi572 – 5833PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi606 – 6174PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Calcium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-3371571. HSF1-dependent transactivation.
R-ATH-4086398. Ca2+ pathway.
R-ATH-442729. CREB phosphorylation through the activation of CaMKII.
R-ATH-5578775. Ion homeostasis.
R-ATH-936837. Ion transport by P-type ATPases.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-dependent protein kinase 2 (EC:2.7.11.1)
Alternative name(s):
Calmodulin-domain protein kinase CDPK isoform 2
Gene namesi
Name:CPK2
Ordered Locus Names:At3g10660
ORF Names:F13M14.5, F18K10.28
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G10660.
TAIRilocus:2075885. AT3G10660.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi2G → A: Decreases membrane association by approximately 50%. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00003633292 – 646Calcium-dependent protein kinase 2Add BLAST645

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine2 Publications1
Lipidationi5S-palmitoyl cysteineCurated1
Modified residuei350PhosphoserineBy similarity1

Keywords - PTMi

Lipoprotein, Myristate, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiQ38870.
PRIDEiQ38870.

PTM databases

iPTMnetiQ38870.

Expressioni

Gene expression databases

ExpressionAtlasiQ38870. baseline and differential.
GenevisibleiQ38870. AT.

Interactioni

Subunit structurei

Interacts with 14-3-3 proteins.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi5569. 1 interactor.
STRINGi3702.AT3G10660.1.

Structurei

3D structure databases

ProteinModelPortaliQ38870.
SMRiQ38870.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini186 – 444Protein kinasePROSITE-ProRule annotationAdd BLAST259
Domaini487 – 522EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini523 – 558EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini559 – 594EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini595 – 628EF-hand 4PROSITE-ProRule annotationAdd BLAST34

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni450 – 480Autoinhibitory domainBy similarityAdd BLAST31

Domaini

There are 3 contiguous domains conserved in the CDPK subfamily: a kinase domain, an autoinhibitory (junction) domain and a calmodulin-like domain. The autoinhibitory domain (450-480) inactivates kinase activity under calcium-free conditions (By similarity).By similarity

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
HOGENOMiHOG000233030.
InParanoidiQ38870.
KOiK13412.
OMAiGHENITF.
OrthoDBiEOG093605DK.
PhylomeDBiQ38870.

Family and domain databases

CDDicd00051. EFh. 2 hits.
InterProiView protein in InterPro
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF13499. EF-hand_7. 2 hits.
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00054. EFh. 4 hits.
SM00220. S_TKc. 1 hit.
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q38870-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNACVGPNI SGNGFLQTVT AAMWRPRIGA EQASSSSHGN GQVSKEAASE
60 70 80 90 100
PATDQVQNKP PEPITMPSSK TNPETKLKPD LEIQPEEKKE KVLAEETKQK
110 120 130 140 150
VVPEESKQEV PPEESKREVV VQPESAKPET KSESKPETTK PETTSETKPE
160 170 180 190 200
TKAEPQKPKH MRRVSSAGLR TESVLQRKTE NFKEFYSLGR KLGQGQFGTT
210 220 230 240 250
FLCLEKGTGN EYACKSISKR KLLTDEDVED VRREIQIMHH LAGHPNVISI
260 270 280 290 300
KGAYEDVVAV HLVMELCSGG ELFDRIIQRG HYTERKAAEL ARTIVGVLEA
310 320 330 340 350
CHSLGVMHRD LKPENFLFVS REEDSLLKTI DFGLSMFFKP DEVFTDVVGS
360 370 380 390 400
PYYVAPEVLR KRYGPESDVW SAGVIVYILL SGVPPFWAET EQGIFEQVLH
410 420 430 440 450
GDLDFSSDPW PSISESAKDL VRKMLVRDPK RRLTAHQVLC HPWVQIDGVA
460 470 480 490 500
PDKPLDSAVL SRMKQFSAMN KFKKMALRVI AESLSEEEIA GLKQMFKMID
510 520 530 540 550
ADNSGQITFE ELKAGLKRVG ANLKESEILD LMQAADVDNS GTIDYKEFIA
560 570 580 590 600
ATLHLNKIER EDHLFAAFSY FDKDESGFIT PDELQQACEE FGVEDARIEE
610 620 630 640
MMRDVDQDKD GRIDYNEFVA MMQKGSIMGG PVKMGLENSI SISLKH
Length:646
Mass (Da):72,254
Last modified:November 1, 1996 - v1
Checksum:iAD6AA8282B81729F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U31833 mRNA. Translation: AAB03244.1.
AF286222 Genomic DNA. Translation: AAG00535.1.
AC011560 Genomic DNA. Translation: AAG51400.1.
AC013428 Genomic DNA. Translation: AAF76372.1.
CP002686 Genomic DNA. Translation: AEE74940.1.
CP002686 Genomic DNA. Translation: ANM63990.1.
BT046185 mRNA. Translation: ACI49784.1.
RefSeqiNP_001326044.1. NM_001337889.1.
NP_187677.1. NM_111902.2.
UniGeneiAt.9827.

Genome annotation databases

EnsemblPlantsiAT3G10660.1; AT3G10660.1; AT3G10660.
AT3G10660.2; AT3G10660.2; AT3G10660.
GeneIDi820235.
GrameneiAT3G10660.1; AT3G10660.1; AT3G10660.
AT3G10660.2; AT3G10660.2; AT3G10660.
KEGGiath:AT3G10660.

Similar proteinsi

Entry informationi

Entry nameiCDPK2_ARATH
AccessioniPrimary (citable) accession number: Q38870
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: November 1, 1996
Last modified: September 27, 2017
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families