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Protein

Calcium-dependent protein kinase 2

Gene

CPK2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in signal transduction pathways that involve calcium as a second messenger.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by calcium. Autophosphorylation may play an important role in the regulation of the kinase activity (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei215ATPPROSITE-ProRule annotation1
Active sitei310Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi192 – 200ATPPROSITE-ProRule annotation9
Calcium bindingi500 – 5111PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi536 – 5472PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi572 – 5833PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi606 – 6174PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Calcium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-dependent protein kinase 2 (EC:2.7.11.1)
Alternative name(s):
Calmodulin-domain protein kinase CDPK isoform 2
Gene namesi
Name:CPK2
Ordered Locus Names:At3g10660
ORF Names:F13M14.5, F18K10.28
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G10660.

Subcellular locationi

  • Endoplasmic reticulum membrane 1 Publication; Lipid-anchor 1 Publication

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: TAIR
  • nucleus Source: GO_Central
  • plasma membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi2G → A: Decreases membrane association by approximately 50%. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00003633292 – 646Calcium-dependent protein kinase 2Add BLAST645

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine2 Publications1
Modified residuei3Deamidated asparagineCurated1
Lipidationi5S-palmitoyl cysteineCurated1

Keywords - PTMi

Lipoprotein, Myristate, Palmitate

Proteomic databases

PaxDbiQ38870.
PRIDEiQ38870.

PTM databases

iPTMnetiQ38870.

Expressioni

Gene expression databases

GenevisibleiQ38870. AT.

Interactioni

Subunit structurei

Interacts with 14-3-3 proteins.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi5569. 1 interactor.
STRINGi3702.AT3G10660.1.

Structurei

3D structure databases

ProteinModelPortaliQ38870.
SMRiQ38870.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini186 – 444Protein kinasePROSITE-ProRule annotationAdd BLAST259
Domaini487 – 522EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini523 – 558EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini559 – 594EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini595 – 628EF-hand 4PROSITE-ProRule annotationAdd BLAST34

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni450 – 480Autoinhibitory domainBy similarityAdd BLAST31

Domaini

There is 3 contiguous domains conserved in the CDPK subfamily: a kinase domain, an autoinhibitory (junction) domain and a calmodulin-like domain. The autoinhibitory domain (450-480) inactivates kinase activity under calcium-free conditions (By similarity).By similarity

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.PROSITE-ProRule annotation
Contains 4 EF-hand domains.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
HOGENOMiHOG000233030.
InParanoidiQ38870.
KOiK13412.
OMAiFDEVLHG.
OrthoDBiEOG093605DK.
PhylomeDBiQ38870.

Family and domain databases

CDDicd00051. EFh. 2 hits.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q38870-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNACVGPNI SGNGFLQTVT AAMWRPRIGA EQASSSSHGN GQVSKEAASE
60 70 80 90 100
PATDQVQNKP PEPITMPSSK TNPETKLKPD LEIQPEEKKE KVLAEETKQK
110 120 130 140 150
VVPEESKQEV PPEESKREVV VQPESAKPET KSESKPETTK PETTSETKPE
160 170 180 190 200
TKAEPQKPKH MRRVSSAGLR TESVLQRKTE NFKEFYSLGR KLGQGQFGTT
210 220 230 240 250
FLCLEKGTGN EYACKSISKR KLLTDEDVED VRREIQIMHH LAGHPNVISI
260 270 280 290 300
KGAYEDVVAV HLVMELCSGG ELFDRIIQRG HYTERKAAEL ARTIVGVLEA
310 320 330 340 350
CHSLGVMHRD LKPENFLFVS REEDSLLKTI DFGLSMFFKP DEVFTDVVGS
360 370 380 390 400
PYYVAPEVLR KRYGPESDVW SAGVIVYILL SGVPPFWAET EQGIFEQVLH
410 420 430 440 450
GDLDFSSDPW PSISESAKDL VRKMLVRDPK RRLTAHQVLC HPWVQIDGVA
460 470 480 490 500
PDKPLDSAVL SRMKQFSAMN KFKKMALRVI AESLSEEEIA GLKQMFKMID
510 520 530 540 550
ADNSGQITFE ELKAGLKRVG ANLKESEILD LMQAADVDNS GTIDYKEFIA
560 570 580 590 600
ATLHLNKIER EDHLFAAFSY FDKDESGFIT PDELQQACEE FGVEDARIEE
610 620 630 640
MMRDVDQDKD GRIDYNEFVA MMQKGSIMGG PVKMGLENSI SISLKH
Length:646
Mass (Da):72,254
Last modified:November 1, 1996 - v1
Checksum:iAD6AA8282B81729F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U31833 mRNA. Translation: AAB03244.1.
AF286222 Genomic DNA. Translation: AAG00535.1.
AC011560 Genomic DNA. Translation: AAG51400.1.
AC013428 Genomic DNA. Translation: AAF76372.1.
CP002686 Genomic DNA. Translation: AEE74940.1.
BT046185 mRNA. Translation: ACI49784.1.
RefSeqiNP_187677.1. NM_111902.2.
UniGeneiAt.9827.

Genome annotation databases

EnsemblPlantsiAT3G10660.1; AT3G10660.1; AT3G10660.
GeneIDi820235.
GrameneiAT3G10660.1; AT3G10660.1; AT3G10660.
KEGGiath:AT3G10660.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U31833 mRNA. Translation: AAB03244.1.
AF286222 Genomic DNA. Translation: AAG00535.1.
AC011560 Genomic DNA. Translation: AAG51400.1.
AC013428 Genomic DNA. Translation: AAF76372.1.
CP002686 Genomic DNA. Translation: AEE74940.1.
BT046185 mRNA. Translation: ACI49784.1.
RefSeqiNP_187677.1. NM_111902.2.
UniGeneiAt.9827.

3D structure databases

ProteinModelPortaliQ38870.
SMRiQ38870.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi5569. 1 interactor.
STRINGi3702.AT3G10660.1.

PTM databases

iPTMnetiQ38870.

Proteomic databases

PaxDbiQ38870.
PRIDEiQ38870.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G10660.1; AT3G10660.1; AT3G10660.
GeneIDi820235.
GrameneiAT3G10660.1; AT3G10660.1; AT3G10660.
KEGGiath:AT3G10660.

Organism-specific databases

TAIRiAT3G10660.

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
HOGENOMiHOG000233030.
InParanoidiQ38870.
KOiK13412.
OMAiFDEVLHG.
OrthoDBiEOG093605DK.
PhylomeDBiQ38870.

Miscellaneous databases

PROiQ38870.

Gene expression databases

GenevisibleiQ38870. AT.

Family and domain databases

CDDicd00051. EFh. 2 hits.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCDPK2_ARATH
AccessioniPrimary (citable) accession number: Q38870
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.