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Protein

Calcium-dependent protein kinase 2

Gene

CPK2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in signal transduction pathways that involve calcium as a second messenger.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by calcium. Autophosphorylation may play an important role in the regulation of the kinase activity (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei215 – 2151ATPPROSITE-ProRule annotation
Active sitei310 – 3101Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi192 – 2009ATPPROSITE-ProRule annotation
Calcium bindingi500 – 511121PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi536 – 547122PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi572 – 583123PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi606 – 617124PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Calcium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT3G10660-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-dependent protein kinase 2 (EC:2.7.11.1)
Alternative name(s):
Calmodulin-domain protein kinase CDPK isoform 2
Gene namesi
Name:CPK2
Ordered Locus Names:At3g10660
ORF Names:F13M14.5, F18K10.28
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G10660.

Subcellular locationi

  • Endoplasmic reticulum membrane 1 Publication; Lipid-anchor 1 Publication

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: TAIR
  • plasma membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi2 – 21G → A: Decreases membrane association by approximately 50%. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved
Chaini2 – 646645Calcium-dependent protein kinase 2PRO_0000363329Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine2 Publications
Modified residuei3 – 31Deamidated asparagineCurated
Lipidationi5 – 51S-palmitoyl cysteineCurated

Keywords - PTMi

Lipoprotein, Myristate, Palmitate

Proteomic databases

PaxDbiQ38870.
PRIDEiQ38870.

Expressioni

Gene expression databases

GenevisibleiQ38870. AT.

Interactioni

Subunit structurei

Interacts with 14-3-3 proteins.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi5569. 1 interaction.
STRINGi3702.AT3G10660.1.

Structurei

3D structure databases

ProteinModelPortaliQ38870.
SMRiQ38870. Positions 136-623.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini186 – 444259Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini487 – 52236EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini523 – 55836EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini559 – 59436EF-hand 3PROSITE-ProRule annotationAdd
BLAST
Domaini595 – 62834EF-hand 4PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni450 – 48031Autoinhibitory domainBy similarityAdd
BLAST

Domaini

There is 3 contiguous domains conserved in the CDPK subfamily: a kinase domain, an autoinhibitory (junction) domain and a calmodulin-like domain. The autoinhibitory domain (450-480) inactivates kinase activity under calcium-free conditions (By similarity).By similarity

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.PROSITE-ProRule annotation
Contains 4 EF-hand domains.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
HOGENOMiHOG000233030.
InParanoidiQ38870.
KOiK13412.
OMAiQSPFQPE.
PhylomeDBiQ38870.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q38870-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNACVGPNI SGNGFLQTVT AAMWRPRIGA EQASSSSHGN GQVSKEAASE
60 70 80 90 100
PATDQVQNKP PEPITMPSSK TNPETKLKPD LEIQPEEKKE KVLAEETKQK
110 120 130 140 150
VVPEESKQEV PPEESKREVV VQPESAKPET KSESKPETTK PETTSETKPE
160 170 180 190 200
TKAEPQKPKH MRRVSSAGLR TESVLQRKTE NFKEFYSLGR KLGQGQFGTT
210 220 230 240 250
FLCLEKGTGN EYACKSISKR KLLTDEDVED VRREIQIMHH LAGHPNVISI
260 270 280 290 300
KGAYEDVVAV HLVMELCSGG ELFDRIIQRG HYTERKAAEL ARTIVGVLEA
310 320 330 340 350
CHSLGVMHRD LKPENFLFVS REEDSLLKTI DFGLSMFFKP DEVFTDVVGS
360 370 380 390 400
PYYVAPEVLR KRYGPESDVW SAGVIVYILL SGVPPFWAET EQGIFEQVLH
410 420 430 440 450
GDLDFSSDPW PSISESAKDL VRKMLVRDPK RRLTAHQVLC HPWVQIDGVA
460 470 480 490 500
PDKPLDSAVL SRMKQFSAMN KFKKMALRVI AESLSEEEIA GLKQMFKMID
510 520 530 540 550
ADNSGQITFE ELKAGLKRVG ANLKESEILD LMQAADVDNS GTIDYKEFIA
560 570 580 590 600
ATLHLNKIER EDHLFAAFSY FDKDESGFIT PDELQQACEE FGVEDARIEE
610 620 630 640
MMRDVDQDKD GRIDYNEFVA MMQKGSIMGG PVKMGLENSI SISLKH
Length:646
Mass (Da):72,254
Last modified:November 1, 1996 - v1
Checksum:iAD6AA8282B81729F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U31833 mRNA. Translation: AAB03244.1.
AF286222 Genomic DNA. Translation: AAG00535.1.
AC011560 Genomic DNA. Translation: AAG51400.1.
AC013428 Genomic DNA. Translation: AAF76372.1.
CP002686 Genomic DNA. Translation: AEE74940.1.
BT046185 mRNA. Translation: ACI49784.1.
RefSeqiNP_187677.1. NM_111902.1.
UniGeneiAt.9827.

Genome annotation databases

EnsemblPlantsiAT3G10660.1; AT3G10660.1; AT3G10660.
GeneIDi820235.
GrameneiAT3G10660.1; AT3G10660.1; AT3G10660.
KEGGiath:AT3G10660.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U31833 mRNA. Translation: AAB03244.1.
AF286222 Genomic DNA. Translation: AAG00535.1.
AC011560 Genomic DNA. Translation: AAG51400.1.
AC013428 Genomic DNA. Translation: AAF76372.1.
CP002686 Genomic DNA. Translation: AEE74940.1.
BT046185 mRNA. Translation: ACI49784.1.
RefSeqiNP_187677.1. NM_111902.1.
UniGeneiAt.9827.

3D structure databases

ProteinModelPortaliQ38870.
SMRiQ38870. Positions 136-623.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi5569. 1 interaction.
STRINGi3702.AT3G10660.1.

Proteomic databases

PaxDbiQ38870.
PRIDEiQ38870.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G10660.1; AT3G10660.1; AT3G10660.
GeneIDi820235.
GrameneiAT3G10660.1; AT3G10660.1; AT3G10660.
KEGGiath:AT3G10660.

Organism-specific databases

TAIRiAT3G10660.

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
HOGENOMiHOG000233030.
InParanoidiQ38870.
KOiK13412.
OMAiQSPFQPE.
PhylomeDBiQ38870.

Enzyme and pathway databases

BioCyciARA:AT3G10660-MONOMER.

Miscellaneous databases

PROiQ38870.

Gene expression databases

GenevisibleiQ38870. AT.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of eight new members of the calmodulin-like domain protein kinase gene family from Arabidopsis thaliana."
    Hrabak E.M., Dickmann L.J., Satterlee J.S., Sussman M.R.
    Plant Mol. Biol. 31:405-412(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. "An Arabidopsis calcium-dependent protein kinase is associated with the endoplasmic reticulum."
    Lu S.X., Hrabak E.M.
    Plant Physiol. 128:1008-1021(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MYRISTOYLATION, SUBCELLULAR LOCATION, MUTAGENESIS OF GLY-2.
  3. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Arabidopsis ORF clones."
    De Los Reyes C., Quan R., Chen H., Bautista V.R., Kim C.J., Ecker J.R.
    Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Phosphorylation-dependent interactions between enzymes of plant metabolism and 14-3-3 proteins."
    Moorhead G., Douglas P., Cotelle V., Harthill J., Morrice N., Meek S., Deiting U., Stitt M., Scarabel M., Aitken A., MacKintosh C.
    Plant J. 18:1-12(1999)
    Cited for: INTERACTION WITH 14-3-3 PROTEINS.
  7. "The CDPK superfamily of protein kinases."
    Harmon A.C., Gribskov M., Gubrium E., Harper J.F.
    New Phytol. 151:175-183(2001)
    Cited for: GENE FAMILY, NOMENCLATURE.
  8. "Calcium signaling through protein kinases. The Arabidopsis calcium-dependent protein kinase gene family."
    Cheng S.-H., Willmann M.R., Chen H.-C., Sheen J.
    Plant Physiol. 129:469-485(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  9. "Unexpected protein families including cell defense components feature in the N-myristoylome of a higher eukaryote."
    Boisson B., Giglione C., Meinnel T.
    J. Biol. Chem. 278:43418-43429(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: MYRISTOYLATION AT GLY-2.
  10. Cited for: GENE FAMILY, NOMENCLATURE.
  11. "Experimental testing of predicted myristoylation targets involved in asymmetric cell division and calcium-dependent signalling."
    Benetka W., Mehlmer N., Maurer-Stroh S., Sammer M., Koranda M., Neumueller R., Betschinger J., Knoblich J.A., Teige M., Eisenhaber F.
    Cell Cycle 7:3709-3719(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: MYRISTOYLATION.

Entry informationi

Entry nameiCDPK2_ARATH
AccessioniPrimary (citable) accession number: Q38870
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.