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Protein

Calcium-dependent protein kinase 4

Gene

CPK4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in signal transduction pathways that involve calcium as a second messenger. Functions as regulator of the calcium-mediated abscisic acid (ABA) signaling pathway. Phosphorylates ABA-responsive transcription factors ABF1 and ABF4 in vitro. Phosphorylates the nuclear zinc finger Di19 in vitro.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by calcium. Autophosphorylation may play an important role in the regulation of the kinase activity (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei54ATPPROSITE-ProRule annotation1
Active sitei149Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi31 – 39ATPPROSITE-ProRule annotation9
Calcium bindingi339 – 3501PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi375 – 3862PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi411 – 4223PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi445 – 4564PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Calcium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-dependent protein kinase 4 (EC:2.7.11.1)
Alternative name(s):
Calmodulin-domain protein kinase CDPK isoform 4
Gene namesi
Name:CPK4
Ordered Locus Names:At4g09570
ORF Names:T25P22.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G09570.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: TAIR
  • nucleus Source: GO_Central
  • plasma membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mutant cpk4-1 shows reduced ABA and salt responsiveness in seed germination.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003045101 – 501Calcium-dependent protein kinase 4Add BLAST501

Proteomic databases

PaxDbiQ38869.
PRIDEiQ38869.

PTM databases

iPTMnetiQ38869.

Expressioni

Gene expression databases

GenevisibleiQ38869. AT.

Interactioni

Subunit structurei

Interacts with Di19.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
At5g21940Q9C5933EBI-979475,EBI-2213247
BT2Q94BN07EBI-979475,EBI-540986
DI19-1Q390835EBI-979475,EBI-979339
dl3455wO233383EBI-979475,EBI-2296482
GAE6Q9LIS34EBI-979475,EBI-2297116
TOC33O236805EBI-979475,EBI-639377

GO - Molecular functioni

Protein-protein interaction databases

BioGridi11840. 32 interactors.
IntActiQ38869. 33 interactors.
MINTiMINT-1788946.
STRINGi3702.AT4G09570.1.

Structurei

3D structure databases

ProteinModelPortaliQ38869.
SMRiQ38869.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 283Protein kinasePROSITE-ProRule annotationAdd BLAST259
Domaini326 – 361EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini362 – 397EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini398 – 433EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini434 – 467EF-hand 4PROSITE-ProRule annotationAdd BLAST34

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni289 – 319Autoinhibitory domainBy similarityAdd BLAST31

Domaini

There is 3 contiguous domains conserved in the CDPK subfamily: a kinase domain, an autoinhibitory (junction) domain and a calmodulin-like domain. The autoinhibitory domain (289-319) inactivates kinase activity under calcium-free conditions (By similarity).By similarity

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.PROSITE-ProRule annotation
Contains 4 EF-hand domains.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
HOGENOMiHOG000233030.
InParanoidiQ38869.
KOiK13412.
OMAiQQACTEF.
OrthoDBiEOG093609BM.
PhylomeDBiQ38869.

Family and domain databases

CDDicd00051. EFh. 2 hits.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q38869-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKPNPRRPS NSVLPYETPR LRDHYLLGKK LGQGQFGTTY LCTEKSSSAN
60 70 80 90 100
YACKSIPKRK LVCREDYEDV WREIQIMHHL SEHPNVVRIK GTYEDSVFVH
110 120 130 140 150
IVMEVCEGGE LFDRIVSKGC FSEREAAKLI KTILGVVEAC HSLGVMHRDL
160 170 180 190 200
KPENFLFDSP SDDAKLKATD FGLSVFYKPG QYLYDVVGSP YYVAPEVLKK
210 220 230 240 250
CYGPEIDVWS AGVILYILLS GVPPFWAETE SGIFRQILQG KIDFKSDPWP
260 270 280 290 300
TISEGAKDLI YKMLDRSPKK RISAHEALCH PWIVDEHAAP DKPLDPAVLS
310 320 330 340 350
RLKQFSQMNK IKKMALRVIA ERLSEEEIGG LKELFKMIDT DNSGTITFEE
360 370 380 390 400
LKAGLKRVGS ELMESEIKSL MDAADIDNSG TIDYGEFLAA TLHINKMERE
410 420 430 440 450
ENLVVAFSYF DKDGSGYITI DELQQACTEF GLCDTPLDDM IKEIDLDNDG
460 470 480 490 500
KIDFSEFTAM MKKGDGVGRS RTMRNNLNFN IAEAFGVEDT SSTAKSDDSP

K
Length:501
Mass (Da):56,416
Last modified:November 1, 1996 - v1
Checksum:iC709C17DFAF74B70
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U31752 mRNA. Translation: AAB03243.1.
AL161515 Genomic DNA. Translation: CAB78080.1.
AL161831 Genomic DNA. Translation: CAB82124.1.
CP002687 Genomic DNA. Translation: AEE82766.1.
PIRiG85097.
RefSeqiNP_192695.1. NM_117025.6.
UniGeneiAt.42950.

Genome annotation databases

EnsemblPlantsiAT4G09570.1; AT4G09570.1; AT4G09570.
GeneIDi826541.
GrameneiAT4G09570.1; AT4G09570.1; AT4G09570.
KEGGiath:AT4G09570.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U31752 mRNA. Translation: AAB03243.1.
AL161515 Genomic DNA. Translation: CAB78080.1.
AL161831 Genomic DNA. Translation: CAB82124.1.
CP002687 Genomic DNA. Translation: AEE82766.1.
PIRiG85097.
RefSeqiNP_192695.1. NM_117025.6.
UniGeneiAt.42950.

3D structure databases

ProteinModelPortaliQ38869.
SMRiQ38869.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi11840. 32 interactors.
IntActiQ38869. 33 interactors.
MINTiMINT-1788946.
STRINGi3702.AT4G09570.1.

PTM databases

iPTMnetiQ38869.

Proteomic databases

PaxDbiQ38869.
PRIDEiQ38869.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G09570.1; AT4G09570.1; AT4G09570.
GeneIDi826541.
GrameneiAT4G09570.1; AT4G09570.1; AT4G09570.
KEGGiath:AT4G09570.

Organism-specific databases

TAIRiAT4G09570.

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
HOGENOMiHOG000233030.
InParanoidiQ38869.
KOiK13412.
OMAiQQACTEF.
OrthoDBiEOG093609BM.
PhylomeDBiQ38869.

Miscellaneous databases

PROiQ38869.

Gene expression databases

GenevisibleiQ38869. AT.

Family and domain databases

CDDicd00051. EFh. 2 hits.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCDPK4_ARATH
AccessioniPrimary (citable) accession number: Q38869
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.