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Protein

Calcium-dependent protein kinase 4

Gene

CPK4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May play a role in signal transduction pathways that involve calcium as a second messenger. Functions as regulator of the calcium-mediated abscisic acid (ABA) signaling pathway. Phosphorylates ABA-responsive transcription factors ABF1 and ABF4 in vitro. Phosphorylates the nuclear zinc finger Di19 in vitro.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by calcium. Autophosphorylation may play an important role in the regulation of the kinase activity (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei54ATPPROSITE-ProRule annotation1
Active sitei149Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi31 – 39ATPPROSITE-ProRule annotation9
Calcium bindingi339 – 3501PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi375 – 3862PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi411 – 4223PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi445 – 4564PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Calcium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-3371571 HSF1-dependent transactivation
R-ATH-4086398 Ca2+ pathway
R-ATH-442729 CREB phosphorylation through the activation of CaMKII
R-ATH-5578775 Ion homeostasis
R-ATH-936837 Ion transport by P-type ATPases

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-dependent protein kinase 4 (EC:2.7.11.1)
Alternative name(s):
Calmodulin-domain protein kinase CDPK isoform 4
Gene namesi
Name:CPK4
Ordered Locus Names:At4g09570
ORF Names:T25P22.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G09570
TAIRilocus:2136917 AT4G09570

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mutant cpk4-1 shows reduced ABA and salt responsiveness in seed germination.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003045101 – 501Calcium-dependent protein kinase 4Add BLAST501

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei189PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ38869
PRIDEiQ38869

PTM databases

iPTMnetiQ38869

Expressioni

Gene expression databases

ExpressionAtlasiQ38869 baseline and differential
GenevisibleiQ38869 AT

Interactioni

Subunit structurei

Interacts with Di19.1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi11840, 32 interactors
IntActiQ38869, 33 interactors
MINTiQ38869
STRINGi3702.AT4G09570.1

Structurei

3D structure databases

ProteinModelPortaliQ38869
SMRiQ38869
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 283Protein kinasePROSITE-ProRule annotationAdd BLAST259
Domaini326 – 361EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini362 – 397EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini398 – 433EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini434 – 467EF-hand 4PROSITE-ProRule annotationAdd BLAST34

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni289 – 319Autoinhibitory domainBy similarityAdd BLAST31

Domaini

There are 3 contiguous domains conserved in the CDPK subfamily: a kinase domain, an autoinhibitory (junction) domain and a calmodulin-like domain. The autoinhibitory domain (289-319) inactivates kinase activity under calcium-free conditions (By similarity).By similarity

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0032 Eukaryota
ENOG410XRMJ LUCA
HOGENOMiHOG000233030
InParanoidiQ38869
KOiK13412
OMAiRTMRSNL
OrthoDBiEOG093609BM
PhylomeDBiQ38869

Family and domain databases

InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF13499 EF-hand_7, 2 hits
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00054 EFh, 4 hits
SM00220 S_TKc, 1 hit
SUPFAMiSSF47473 SSF47473, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 4 hits
PS50222 EF_HAND_2, 4 hits
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

Q38869-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKPNPRRPS NSVLPYETPR LRDHYLLGKK LGQGQFGTTY LCTEKSSSAN
60 70 80 90 100
YACKSIPKRK LVCREDYEDV WREIQIMHHL SEHPNVVRIK GTYEDSVFVH
110 120 130 140 150
IVMEVCEGGE LFDRIVSKGC FSEREAAKLI KTILGVVEAC HSLGVMHRDL
160 170 180 190 200
KPENFLFDSP SDDAKLKATD FGLSVFYKPG QYLYDVVGSP YYVAPEVLKK
210 220 230 240 250
CYGPEIDVWS AGVILYILLS GVPPFWAETE SGIFRQILQG KIDFKSDPWP
260 270 280 290 300
TISEGAKDLI YKMLDRSPKK RISAHEALCH PWIVDEHAAP DKPLDPAVLS
310 320 330 340 350
RLKQFSQMNK IKKMALRVIA ERLSEEEIGG LKELFKMIDT DNSGTITFEE
360 370 380 390 400
LKAGLKRVGS ELMESEIKSL MDAADIDNSG TIDYGEFLAA TLHINKMERE
410 420 430 440 450
ENLVVAFSYF DKDGSGYITI DELQQACTEF GLCDTPLDDM IKEIDLDNDG
460 470 480 490 500
KIDFSEFTAM MKKGDGVGRS RTMRNNLNFN IAEAFGVEDT SSTAKSDDSP

K
Length:501
Mass (Da):56,416
Last modified:November 1, 1996 - v1
Checksum:iC709C17DFAF74B70
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U31752 mRNA Translation: AAB03243.1
AL161515 Genomic DNA Translation: CAB78080.1
AL161831 Genomic DNA Translation: CAB82124.1
CP002687 Genomic DNA Translation: AEE82766.1
PIRiG85097
RefSeqiNP_192695.1, NM_117025.6
UniGeneiAt.42950

Genome annotation databases

EnsemblPlantsiAT4G09570.1; AT4G09570.1; AT4G09570
GeneIDi826541
GrameneiAT4G09570.1; AT4G09570.1; AT4G09570
KEGGiath:AT4G09570

Similar proteinsi

Entry informationi

Entry nameiCDPK4_ARATH
AccessioniPrimary (citable) accession number: Q38869
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: November 1, 1996
Last modified: April 25, 2018
This is version 147 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health