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Q38858

- CALR2_ARATH

UniProt

Q38858 - CALR2_ARATH

Protein

Calreticulin-2

Gene

CRT2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 3 (29 May 2007)
      Previous versions | rss
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    Functioni

    Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin may interact transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei112 – 1121CarbohydrateBy similarity
    Binding sitei114 – 1141CarbohydrateBy similarity
    Binding sitei131 – 1311CarbohydrateBy similarity
    Binding sitei138 – 1381CarbohydrateBy similarity
    Binding sitei321 – 3211CarbohydrateBy similarity

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro

    GO - Biological processi

    1. protein folding Source: InterPro
    2. response to oxidative stress Source: TAIR
    3. response to salt stress Source: TAIR

    Keywords - Molecular functioni

    Chaperone

    Keywords - Ligandi

    Calcium, Lectin, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Calreticulin-2
    Gene namesi
    Name:CRT2
    Synonyms:CRTL
    Ordered Locus Names:At1g09210
    ORF Names:T12M4.8
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G09210.

    Subcellular locationi

    Endoplasmic reticulum lumen PROSITE-ProRule annotation

    GO - Cellular componenti

    1. apoplast Source: TAIR
    2. endoplasmic reticulum Source: TAIR
    3. endoplasmic reticulum lumen Source: UniProtKB-SubCell
    4. mitochondrion Source: TAIR
    5. vacuole Source: TAIR

    Keywords - Cellular componenti

    Endoplasmic reticulum

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222Sequence AnalysisAdd
    BLAST
    Chaini23 – 424402Calreticulin-2PRO_0000004185Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi59 – 591N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi108 ↔ 140By similarity
    Modified residuei381 – 3811PhosphoserineBy similarity
    Modified residuei396 – 3961PhosphoserineBy similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiQ38858.
    PRIDEiQ38858.

    2D gel databases

    SWISS-2DPAGEQ38858.

    Expressioni

    Gene expression databases

    ArrayExpressiQ38858.
    GenevestigatoriQ38858.

    Interactioni

    Protein-protein interaction databases

    BioGridi22682. 4 interactions.
    STRINGi3702.AT1G09210.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ38858.
    SMRiQ38858. Positions 23-372.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati194 – 205121-1Add
    BLAST
    Repeati213 – 224121-2Add
    BLAST
    Repeati230 – 241121-3Add
    BLAST
    Repeati248 – 259121-4Add
    BLAST
    Repeati263 – 273112-1Add
    BLAST
    Repeati277 – 287112-2Add
    BLAST
    Repeati291 – 301112-3Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni194 – 259664 X approximate repeatsAdd
    BLAST
    Regioni263 – 301393 X approximate repeatsAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi421 – 4244Prevents secretion from ERPROSITE-ProRule annotation

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi355 – 41763Asp/Glu/Lys-richAdd
    BLAST

    Domaini

    Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity By similarity.By similarity
    The interaction with glycans occurs through a binding site in the globular lectin domain.By similarity
    The zinc binding sites are localized to the N-domain.By similarity

    Sequence similaritiesi

    Belongs to the calreticulin family.Curated

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG305105.
    HOGENOMiHOG000192435.
    InParanoidiQ38858.
    KOiK08057.
    OMAiRWVNSKH.
    PhylomeDBiQ38858.

    Family and domain databases

    Gene3Di2.10.250.10. 1 hit.
    2.60.120.200. 1 hit.
    InterProiIPR001580. Calret/calnex.
    IPR018124. Calret/calnex_CS.
    IPR009169. Calreticulin.
    IPR009033. Calreticulin/calnexin_P_dom.
    IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    [Graphical view]
    PANTHERiPTHR11073. PTHR11073. 1 hit.
    PfamiPF00262. Calreticulin. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002356. Calreticulin. 1 hit.
    PRINTSiPR00626. CALRETICULIN.
    SUPFAMiSSF49899. SSF49899. 1 hit.
    SSF63887. SSF63887. 1 hit.
    PROSITEiPS00803. CALRETICULIN_1. 1 hit.
    PS00804. CALRETICULIN_2. 1 hit.
    PS00805. CALRETICULIN_REPEAT. 2 hits.
    PS00014. ER_TARGET. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q38858-1 [UniParc]FASTAAdd to Basket

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    MAKMIPSLVS LILIGLVAIA SAAVIFEERF DDGWENRWVK SEWKKDDNTA    50
    GEWKHTAGNW SGDANDKGIQ TSEDYRFYAI SAEFPEFSNK DKTLVFQFSV 100
    KHEQKLDCGG GYMKLLSGDV DQKKFGGDTP YSIMFGPDIC GYSTKKVHAI 150
    LTYNEANHLI KKDVPCETDQ LTHVYTFILR PDATYSILID NVEKQTGSLY 200
    SDWDLLPPKK IKDPSAKKPE DWDEQEYISD PEDKKPDGYD DIPKEIPDTD 250
    SKKPEDWDDE EDGEWTAPTI PNPEYMGEWK PKQIKNPNYK GKWEAPLIDN 300
    PDFKDDPELY VFPKLKYVGL ELWQVKSGSL FDNVLICDDP DYAKKLADET 350
    WGKLKDAEKA AFDEAEKKNE EEESKDAPAE SDAEDEPEDD EGGDDSDSES 400
    KAEETKSVDS EETSEKDATA HDEL 424
    Length:424
    Mass (Da):48,157
    Last modified:May 29, 2007 - v3
    Checksum:i51421329AE81A7F5
    GO

    Sequence cautioni

    The sequence AAC49696.1 differs from that shown. Reason: Erroneous initiation.
    The sequence AAC24083.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti16 – 194LVAI → NSAR in AAA80652. 1 PublicationCurated
    Sequence conflicti155 – 1551E → G in AAC49696. (PubMed:9159940)Curated
    Sequence conflicti155 – 1551E → G in AAA80652. 1 PublicationCurated
    Sequence conflicti236 – 2361P → T in AAA80652. 1 PublicationCurated
    Sequence conflicti408 – 4081V → E in AAA80652. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC003114 Genomic DNA. Translation: AAC24083.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE28414.1.
    AY045656 mRNA. Translation: AAK74014.1.
    AY059662 mRNA. Translation: AAL31155.1.
    U66344 Genomic DNA. Translation: AAC49696.1. Different initiation.
    U27698 mRNA. Translation: AAA80652.1.
    PIRiH86224.
    RefSeqiNP_172392.1. NM_100791.3.
    UniGeneiAt.1529.

    Genome annotation databases

    EnsemblPlantsiAT1G09210.1; AT1G09210.1; AT1G09210.
    GeneIDi837441.
    KEGGiath:AT1G09210.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC003114 Genomic DNA. Translation: AAC24083.1 . Sequence problems.
    CP002684 Genomic DNA. Translation: AEE28414.1 .
    AY045656 mRNA. Translation: AAK74014.1 .
    AY059662 mRNA. Translation: AAL31155.1 .
    U66344 Genomic DNA. Translation: AAC49696.1 . Different initiation.
    U27698 mRNA. Translation: AAA80652.1 .
    PIRi H86224.
    RefSeqi NP_172392.1. NM_100791.3.
    UniGenei At.1529.

    3D structure databases

    ProteinModelPortali Q38858.
    SMRi Q38858. Positions 23-372.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 22682. 4 interactions.
    STRINGi 3702.AT1G09210.1-P.

    2D gel databases

    SWISS-2DPAGE Q38858.

    Proteomic databases

    PaxDbi Q38858.
    PRIDEi Q38858.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G09210.1 ; AT1G09210.1 ; AT1G09210 .
    GeneIDi 837441.
    KEGGi ath:AT1G09210.

    Organism-specific databases

    TAIRi AT1G09210.

    Phylogenomic databases

    eggNOGi NOG305105.
    HOGENOMi HOG000192435.
    InParanoidi Q38858.
    KOi K08057.
    OMAi RWVNSKH.
    PhylomeDBi Q38858.

    Gene expression databases

    ArrayExpressi Q38858.
    Genevestigatori Q38858.

    Family and domain databases

    Gene3Di 2.10.250.10. 1 hit.
    2.60.120.200. 1 hit.
    InterProi IPR001580. Calret/calnex.
    IPR018124. Calret/calnex_CS.
    IPR009169. Calreticulin.
    IPR009033. Calreticulin/calnexin_P_dom.
    IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    [Graphical view ]
    PANTHERi PTHR11073. PTHR11073. 1 hit.
    Pfami PF00262. Calreticulin. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002356. Calreticulin. 1 hit.
    PRINTSi PR00626. CALRETICULIN.
    SUPFAMi SSF49899. SSF49899. 1 hit.
    SSF63887. SSF63887. 1 hit.
    PROSITEi PS00803. CALRETICULIN_1. 1 hit.
    PS00804. CALRETICULIN_2. 1 hit.
    PS00805. CALRETICULIN_REPEAT. 2 hits.
    PS00014. ER_TARGET. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Abundant accumulation of the calcium-binding molecular chaperone calreticulin in specific floral tissues of Arabidopsis thaliana."
      Nelson D.E., Glaunsinger B., Bohnert H.J.
      Plant Physiol. 114:29-37(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-174.
    5. "Nucleotide sequence of an Arabidopsis thaliana cDNA encoding a protein homologous to plant and animal calreticulins."
      Benedetti C.E., Turner J.G.
      Plant Gene Register PGR95-047
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 16-424.
      Strain: cv. Landsberg erecta.
      Tissue: Flower.
    6. "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
      Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
      Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCALR2_ARATH
    AccessioniPrimary (citable) accession number: Q38858
    Secondary accession number(s): O04152, O80486, Q94AW7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: May 29, 2007
    Last modified: October 1, 2014
    This is version 130 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3