IRT1

Functionⁱ

High-affinity iron transporter that plays a key role in the uptake of iron from the rhizosphere across the plasma membrane in the root epidermal layer. Acts as the principal regulator of iron homeostasis in planta. Also mediates the heavy metals uptake under iron-deficiency by its ability to transport cobalt, cadmium, manganese and/or zinc ions.1 Publication

Miscellaneous

Inhibited by cadmium and Fe²⁺ ions and at 100-fold excess inhibited by cobalt, manganese and zinc ions.

The availability of secondary non-iron metal substrates (Zn, Mn, and Co) controls the localization of IRT1 between the outer polar domain of the plasma membrane and early endosome/trans-Golgi network in root epidermal cells.1 Publication

GO - Molecular functionⁱ

copper uptake transmembrane transporter activity
Source: TAIR
Inferred from genetic interactionⁱ
- 17337631
iron ion transmembrane transporter activity
Source: TAIR
Traceable author statementⁱ
- 10394943
zinc ion transmembrane transporter activity Source: InterPro

Complete GO annotation...

GO - Biological processⁱ

cellular response to ethylene stimulus
Source: TAIR
Inferred from expression patternⁱ
- 20627899
cellular response to iron ion
Source: TAIR
Inferred from expression patternⁱ
- 20627899
cellular response to nitric oxide
Source: TAIR
Inferred from expression patternⁱ
- 20627899
iron ion homeostasis Source: UniProtKB-KW
metal ion transport
Source: TAIR
Inferred from mutant phenotypeⁱ
- 21628566
nickel cation transport
Source: TAIR
Inferred from mutant phenotypeⁱ
- 21742768
response to bacterium
Source: TAIR
Inferred from expression patternⁱ
- 19121106

Complete GO annotation...

Keywordsⁱ

Biological process	Ion transport, Iron transport, Transport
Ligand	Iron

Enzyme and pathway databases

BioCycⁱ	ARA:AT4G19690-MONOMER. MetaCyc:AT4G19690-MONOMER.

BioCycⁱ

ARA:AT4G19690-MONOMER.
MetaCyc:AT4G19690-MONOMER.

Protein family/group databases

TCDBⁱ	2.A.5.1.2. the zinc (zn(2+))-iron (fe(2+)) permease (zip) family.

TCDBⁱ

2.A.5.1.2. the zinc (zn(2+))-iron (fe(2+)) permease (zip) family.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Fe(2+) transport protein 1 Alternative name(s): Fe(II) transport protein 1 Iron-regulated transporter 1
Gene namesⁱ	Name:IRT1 Ordered Locus Names:At4g19690 ORF Names:T16H5.50
Organismⁱ	Arabidopsis thaliana (Mouse-ear cress)
Taxonomic identifierⁱ	3702 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Mesangiospermae › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis
Proteomesⁱ	UP000006548 Componentⁱ: Chromosome 4

Organism-specific databases

Arabidopsis Information Portal More...Araportⁱ	AT4G19690.
TAIRⁱ	locus:2133965. AT4G19690.

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	23 – 52	ExtracellularSequence analysisAdd BLAST	30
Transmembraneⁱ	53 – 73	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	74 – 84	CytoplasmicSequence analysisAdd BLAST	11
Transmembraneⁱ	85 – 105	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	106 – 125	ExtracellularSequence analysisAdd BLAST	20
Transmembraneⁱ	126 – 146	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	147 – 192	CytoplasmicSequence analysisAdd BLAST	46
Transmembraneⁱ	193 – 213	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	214 – 224	ExtracellularSequence analysisAdd BLAST	11
Transmembraneⁱ	225 – 245	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	246 – 254	CytoplasmicSequence analysis	9
Transmembraneⁱ	255 – 275	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	276 – 286	ExtracellularSequence analysisAdd BLAST	11
Transmembraneⁱ	287 – 307	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	308 – 326	CytoplasmicSequence analysisAdd BLAST	19
Transmembraneⁱ	327 – 347	HelicalSequence analysisAdd BLAST	21

Keywords - Cellular componentⁱ

Cell membrane, Endosome, Golgi apparatus, Membrane, Vacuole

Pathology & Biotechⁱ

Disruption phenotypeⁱ

Plants exhibit a lethal chlorotic phenotype.1 Publication

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	104	H → A: Suppresses transport. 1 Publication	1
Mutagenesisⁱ	108	D → A: Abolishes iron and manganese transport. 1 Publication	1
Mutagenesisⁱ	111	E → A: Abolishes zinc transport. Abolishes iron and manganese transport; when associated with A-108. 1 Publication	1
Mutagenesisⁱ	144	D → A: Abolishes iron and manganese transport. Reduces cadmium transport. 1 Publication	1
Mutagenesisⁱ	154	K → R: Suppresses ubiquitination and loss of activity; when associated with R-179. 1 Publication	1
Mutagenesisⁱ	179	K → R: Suppresses ubiquitination and loss of activity; when associated with R-154. 1 Publication	1
Mutagenesisⁱ	205	H → A or E: Suppresses transport. 1 Publication	1
Mutagenesisⁱ	206	S → A: Suppresses transport. 1 Publication	1
Mutagenesisⁱ	232	H → A: Suppresses transport. 1 Publication	1
Mutagenesisⁱ	236	E → A: Suppresses transport. 1 Publication	1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Signal peptideⁱ	1 – 22	Sequence analysisAdd BLAST		22
ChainⁱPRO_0000041636	23 – 347	Fe(2+) transport protein 1Add BLAST		325

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Cross-linkⁱ	154	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linkⁱ	179	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

Post-translational modificationⁱ

Monoubiquitinated on several Lys residues. Monoubiquitination controls trafficking from the plasma membrane and targeting to the vacuole.1 Publication

Keywords - PTMⁱ

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbⁱ	Q38856.

PaxDbⁱ

Q38856.

Expressionⁱ

Tissue specificityⁱ

Expressed in the external cell layers of the root including the lateral branching zone. Also detected in flowers before pollination.1 Publication

Inductionⁱ

In roots by iron starvation.1 Publication

Gene expression databases

ExpressionAtlasⁱ	Q38856. baseline and differential.
Genevisibleⁱ	Q38856. AT.

Interactionⁱ

Subunit structureⁱ

Interacts with FREE1.1 Publication

Protein-protein interaction databases

BioGridⁱ	13006. 3 interactors.
Database of interacting proteins More...DIPⁱ	DIP-60391N.
STRINGⁱ	3702.AT4G19690.2.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q38856.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	205 – 206	Heavy metals binding		2

Sequence similaritiesⁱ

Belongs to the ZIP transporter (TC 2.A.5) family. [View classification]Curated

Keywords - Domainⁱ

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	KOG1558. Eukaryota. ENOG4111GP2. LUCA.
HOGENOMⁱ	HOG000157076.
InParanoidⁱ	Q38856.
KEGG Orthology (KO) More...KOⁱ	K14709.
OMAⁱ	TEAFITH.
Database of Orthologous Groups More...OrthoDBⁱ	EOG09360GCY.

Family and domain databases

InterProⁱ	View protein in InterPro IPR003689. ZIP. IPR004698. Zn/Fe_permease_fun/pln.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF02535. Zip. 1 hit.
TIGRFAMsⁱ	TIGR00820. zip. 1 hit.

Sequences (2)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket Added to basket

Isoform 1 (identifier: Q38856-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASNSALLMK TIFLVLIFVS FAISPATSTA PEECGSESAN PCVNKAKALP 
        60         70         80         90        100
LKVIAIFVIL IASMIGVGAP LFSRNVSFLQ PDGNIFTIIK CFASGIILGT 
       110        120        130        140        150
GFMHVLPDSF EMLSSICLEE NPWHKFPFSG FLAMLSGLIT LAIDSMATSL 
       160        170        180        190        200
YTSKNAVGIM PHGHGHGHGP ANDVTLPIKE DDSSNAQLLR YRVIAMVLEL 
       210        220        230        240        250
GIIVHSVVIG LSLGATSDTC TIKGLIAALC FHQMFEGMGL GGCILQAEYT 
       260        270        280        290        300
NMKKFVMAFF FAVTTPFGIA LGIALSTVYQ DNSPKALITV GLLNACSAGL 
       310        320        330        340 
LIYMALVDLL AAEFMGPKLQ GSIKMQFKCL IAALLGCGGM SIIAKWA

Length:347

Mass (Da):36,727

Last modified:May 31, 2011 - v2

Checksum:ⁱ1F94091668994EA4

GO

Isoform 2 (identifier: Q38856-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
198-211: LELGIIVHSVVIGL → RTHIYTYRISLYFK
212-347: Missing.

« Hide

        10         20         30         40         50
MASNSALLMK TIFLVLIFVS FAISPATSTA PEECGSESAN PCVNKAKALP 
        60         70         80         90        100
LKVIAIFVIL IASMIGVGAP LFSRNVSFLQ PDGNIFTIIK CFASGIILGT 
       110        120        130        140        150
GFMHVLPDSF EMLSSICLEE NPWHKFPFSG FLAMLSGLIT LAIDSMATSL 
       160        170        180        190        200
YTSKNAVGIM PHGHGHGHGP ANDVTLPIKE DDSSNAQLLR YRVIAMVRTH 
       210 
IYTYRISLYF K

Note: May be due to an intron retention.

Show »

Length:211

Mass (Da):22,844

Checksum:ⁱA5D0A901E98F2E61

GO

Sequence cautionⁱ

The sequence AAB01678 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

The sequence CAA19686 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

The sequence CAB78971 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_008361	198 – 211	LELGI…VVIGL → RTHIYTYRISLYFK in isoform 2. 1 PublicationAdd BLAST		14
Alternative sequenceⁱVSP_008362	212 – 347	Missing in isoform 2. 1 PublicationAdd BLAST		136

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AL024486 Genomic DNA. Translation: CAA19686.1. Different initiation. AL161551 Genomic DNA. Translation: CAB78971.1. Different initiation. CP002687 Genomic DNA. Translation: AEE84216.1. CP002687 Genomic DNA. Translation: AEE84217.1. AY087095 mRNA. Translation: AAM64655.1. U27590 mRNA. Translation: AAB01678.1. Different initiation.
PIRⁱ	T04750.
NCBI Reference Sequences More...RefSeqⁱ	NP_567590.3. NM_118089.4. [Q38856-1] NP_849546.1. NM_179215.2. [Q38856-2]
UniGeneⁱ	At.2319.

Genome annotation databases

EnsemblPlantsⁱ	AT4G19690.1; AT4G19690.1; AT4G19690. [Q38856-2] AT4G19690.2; AT4G19690.2; AT4G19690. [Q38856-1]
GeneIDⁱ	827713.
Grameneⁱ	AT4G19690.1; AT4G19690.1; AT4G19690. AT4G19690.2; AT4G19690.2; AT4G19690.
KEGGⁱ	ath:AT4G19690.

Keywords - Coding sequence diversityⁱ

Alternative splicing

Similar proteinsⁱ

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q38856	A0A178UTD9	Arabidopsis thaliana (Mouse-ear cress)	347	UniRef100_Q38856	Cluster: Fe(2+) transport protein 1	2

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q38856	A0A178UTD9 A0A087GFH0 Q1ENG8 Q0VJU1 Q93YA1 R0F9G7 Q70IJ1 A0A1J3IZT2	Arabidopsis thaliana (Mouse-ear cress) Arabis alpina (Alpine rock-cress) Noccaea caerulescens (Alpine penny-cress) (Thlaspi caerulescens) Capsella rubella Arabidopsis halleri subsp. halleri (Arabis halleri)	347	UniRef90_Q38856	Cluster: Fe(2+) transport protein 1	9

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q38856	A0A178UTD9 A0A087GFH0 Q1ENG8 Q0VJU1 Q93YA1 Q70IJ1 R0F9G7 A0A1J3IZT2 R0GLN7 UPI00053B363F +103	Arabidopsis thaliana (Mouse-ear cress) Arabis alpina (Alpine rock-cress) Noccaea caerulescens (Alpine penny-cress) (Thlaspi caerulescens) Arabidopsis halleri subsp. halleri (Arabis halleri) Capsella rubella Camelina sativa (False flax) (Myagrum sativum) Ananas comosus (Pineapple) (Ananas ananas) Fragaria vesca subsp. vesca Nelumbo nucifera (Sacred lotus) Vitis vinifera (Grape) And more	347	UniRef50_Q38856	Cluster: Fe(2+) transport protein 1	114
Q38856-2	A0A022QHS7 K4AT57 A0A022QFP9 M1BWG2 A0A0D3BKI2 A0A0S3R2W7	Erythranthe guttata (Yellow monkey flower) (Mimulus guttatus) Solanum lycopersicum (Tomato) (Lycopersicon esculentum) Solanum tuberosum (Potato) Brassica oleracea var. oleracea Vigna angularis var. angularis	211	UniRef50_Q38856-2	Cluster: Isoform 2 of Fe(2+) transport protein 1	7

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AL024486 Genomic DNA. Translation: CAA19686.1. Different initiation. AL161551 Genomic DNA. Translation: CAB78971.1. Different initiation. CP002687 Genomic DNA. Translation: AEE84216.1. CP002687 Genomic DNA. Translation: AEE84217.1. AY087095 mRNA. Translation: AAM64655.1. U27590 mRNA. Translation: AAB01678.1. Different initiation.
PIRⁱ	T04750.
NCBI Reference Sequences More...RefSeqⁱ	NP_567590.3. NM_118089.4. [Q38856-1] NP_849546.1. NM_179215.2. [Q38856-2]
UniGeneⁱ	At.2319.

3D structure databases

ProteinModelPortalⁱ	Q38856.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	13006. 3 interactors.
Database of interacting proteins More...DIPⁱ	DIP-60391N.
STRINGⁱ	3702.AT4G19690.2.

Protein family/group databases

TCDBⁱ	2.A.5.1.2. the zinc (zn(2+))-iron (fe(2+)) permease (zip) family.

TCDBⁱ

2.A.5.1.2. the zinc (zn(2+))-iron (fe(2+)) permease (zip) family.

Proteomic databases

PaxDbⁱ	Q38856.

PaxDbⁱ

Q38856.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblPlantsⁱ	AT4G19690.1; AT4G19690.1; AT4G19690. [Q38856-2] AT4G19690.2; AT4G19690.2; AT4G19690. [Q38856-1]
GeneIDⁱ	827713.
Grameneⁱ	AT4G19690.1; AT4G19690.1; AT4G19690. AT4G19690.2; AT4G19690.2; AT4G19690.
KEGGⁱ	ath:AT4G19690.

Organism-specific databases

Arabidopsis Information Portal More...Araportⁱ	AT4G19690.
TAIRⁱ	locus:2133965. AT4G19690.

Phylogenomic databases

eggNOGⁱ	KOG1558. Eukaryota. ENOG4111GP2. LUCA.
HOGENOMⁱ	HOG000157076.
InParanoidⁱ	Q38856.
KEGG Orthology (KO) More...KOⁱ	K14709.
OMAⁱ	TEAFITH.
Database of Orthologous Groups More...OrthoDBⁱ	EOG09360GCY.

Enzyme and pathway databases

BioCycⁱ	ARA:AT4G19690-MONOMER. MetaCyc:AT4G19690-MONOMER.

BioCycⁱ

ARA:AT4G19690-MONOMER.
MetaCyc:AT4G19690-MONOMER.

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:Q38856.

PROⁱ

PR:Q38856.

Gene expression databases

ExpressionAtlasⁱ	Q38856. baseline and differential.
Genevisibleⁱ	Q38856. AT.

Family and domain databases

InterProⁱ	View protein in InterPro IPR003689. ZIP. IPR004698. Zn/Fe_permease_fun/pln.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF02535. Zip. 1 hit.
TIGRFAMsⁱ	TIGR00820. zip. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	IRT1_ARATH
Accessionⁱ	Q38856Primary (citable) accession number: Q38856 Secondary accession number(s): Q8LBP0
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	September 26, 2003
	Last sequence update:	May 31, 2011
	Last modified:	August 30, 2017
	This is version 138 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Plant Protein Annotation Program

UniProtKB

UniParc

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UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q38856 (IRT1_ARATH)

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Fe(2+) transport protein 1

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Vacuole

Golgi apparatus

Endosome

Plasma membrane

Endosome

Golgi apparatus

Plasma Membrane

Vacuole

Other locations

Topology

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Proteomic databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (2)i

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Alternative sequence

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequences (2)ⁱ

Sequence cautionⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ