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Protein

Fe(2+) transport protein 1

Gene

IRT1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

High-affinity iron transporter that plays a key role in the uptake of iron from the rhizosphere across the plasma membrane in the root epidermal layer. Acts as the principal regulator of iron homeostasis in planta. Also mediates the heavy metals uptake under iron-deficiency by its ability to transport cobalt, cadmium, manganese and/or zinc ions.1 Publication

GO - Molecular functioni

  • copper uptake transmembrane transporter activity Source: TAIR
  • iron ion transmembrane transporter activity Source: TAIR
  • zinc ion transmembrane transporter activity Source: InterPro

GO - Biological processi

  • cellular response to ethylene stimulus Source: TAIR
  • cellular response to iron ion Source: TAIR
  • cellular response to nitric oxide Source: TAIR
  • iron ion homeostasis Source: UniProtKB-KW
  • metal ion transport Source: TAIR
  • nickel cation transport Source: TAIR
  • response to bacterium Source: TAIR
Complete GO annotation...

Keywords - Biological processi

Ion transport, Iron transport, Transport

Keywords - Ligandi

Iron

Enzyme and pathway databases

BioCyciARA:AT4G19690-MONOMER.
MetaCyc:AT4G19690-MONOMER.

Protein family/group databases

TCDBi2.A.5.1.2. the zinc (zn(2+))-iron (fe(2+)) permease (zip) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Fe(2+) transport protein 1
Alternative name(s):
Fe(II) transport protein 1
Iron-regulated transporter 1
Gene namesi
Name:IRT1
Ordered Locus Names:At4g19690
ORF Names:T16H5.50
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G19690.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini23 – 52ExtracellularSequence analysisAdd BLAST30
Transmembranei53 – 73HelicalSequence analysisAdd BLAST21
Topological domaini74 – 84CytoplasmicSequence analysisAdd BLAST11
Transmembranei85 – 105HelicalSequence analysisAdd BLAST21
Topological domaini106 – 125ExtracellularSequence analysisAdd BLAST20
Transmembranei126 – 146HelicalSequence analysisAdd BLAST21
Topological domaini147 – 192CytoplasmicSequence analysisAdd BLAST46
Transmembranei193 – 213HelicalSequence analysisAdd BLAST21
Topological domaini214 – 224ExtracellularSequence analysisAdd BLAST11
Transmembranei225 – 245HelicalSequence analysisAdd BLAST21
Topological domaini246 – 254CytoplasmicSequence analysis9
Transmembranei255 – 275HelicalSequence analysisAdd BLAST21
Topological domaini276 – 286ExtracellularSequence analysisAdd BLAST11
Transmembranei287 – 307HelicalSequence analysisAdd BLAST21
Topological domaini308 – 326CytoplasmicSequence analysisAdd BLAST19
Transmembranei327 – 347HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • early endosome Source: UniProtKB-SubCell
  • endosome Source: TAIR
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: TAIR
  • trans-Golgi network Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Golgi apparatus, Membrane, Vacuole

Pathology & Biotechi

Disruption phenotypei

Plants exhibit a lethal chlorotic phenotype.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi104H → A: Suppresses transport. 1 Publication1
Mutagenesisi108D → A: Abolishes iron and manganese transport. 1 Publication1
Mutagenesisi111E → A: Abolishes zinc transport. Abolishes iron and manganese transport; when associated with A-108. 1 Publication1
Mutagenesisi144D → A: Abolishes iron and manganese transport. Reduces cadmium transport. 1 Publication1
Mutagenesisi154K → R: Suppresses ubiquitination and loss of activity; when associated with R-179. 1 Publication1
Mutagenesisi179K → R: Suppresses ubiquitination and loss of activity; when associated with R-154. 1 Publication1
Mutagenesisi205H → A or E: Suppresses transport. 1 Publication1
Mutagenesisi206S → A: Suppresses transport. 1 Publication1
Mutagenesisi232H → A: Suppresses transport. 1 Publication1
Mutagenesisi236E → A: Suppresses transport. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000004163623 – 347Fe(2+) transport protein 1Add BLAST325

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki154Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki179Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

Post-translational modificationi

Monoubiquitinated on several Lys residues. Monoubiquitination controls trafficking from the plasma membrane and targeting to the vacuole.1 Publication

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ38856.

Expressioni

Tissue specificityi

Expressed in the external cell layers of the root including the lateral branching zone. Also detected in flowers before pollination.1 Publication

Inductioni

In roots by iron starvation.1 Publication

Gene expression databases

GenevisibleiQ38856. AT.

Interactioni

Subunit structurei

Interacts with FREE1.1 Publication

Protein-protein interaction databases

BioGridi13006. 3 interactors.
DIPiDIP-60391N.
STRINGi3702.AT4G19690.2.

Structurei

3D structure databases

ProteinModelPortaliQ38856.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni205 – 206Heavy metals binding2

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1558. Eukaryota.
ENOG4111GP2. LUCA.
HOGENOMiHOG000157076.
InParanoidiQ38856.
KOiK14709.
OMAiCEHIINT.
OrthoDBiEOG09360GCY.

Family and domain databases

InterProiIPR003689. ZIP.
IPR004698. Zn/Fe_permease_fun/pln.
[Graphical view]
PfamiPF02535. Zip. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00820. zip. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q38856-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASNSALLMK TIFLVLIFVS FAISPATSTA PEECGSESAN PCVNKAKALP
60 70 80 90 100
LKVIAIFVIL IASMIGVGAP LFSRNVSFLQ PDGNIFTIIK CFASGIILGT
110 120 130 140 150
GFMHVLPDSF EMLSSICLEE NPWHKFPFSG FLAMLSGLIT LAIDSMATSL
160 170 180 190 200
YTSKNAVGIM PHGHGHGHGP ANDVTLPIKE DDSSNAQLLR YRVIAMVLEL
210 220 230 240 250
GIIVHSVVIG LSLGATSDTC TIKGLIAALC FHQMFEGMGL GGCILQAEYT
260 270 280 290 300
NMKKFVMAFF FAVTTPFGIA LGIALSTVYQ DNSPKALITV GLLNACSAGL
310 320 330 340
LIYMALVDLL AAEFMGPKLQ GSIKMQFKCL IAALLGCGGM SIIAKWA
Length:347
Mass (Da):36,727
Last modified:May 31, 2011 - v2
Checksum:i1F94091668994EA4
GO
Isoform 2 (identifier: Q38856-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     198-211: LELGIIVHSVVIGL → RTHIYTYRISLYFK
     212-347: Missing.

Note: May be due to an intron retention.
Show »
Length:211
Mass (Da):22,844
Checksum:iA5D0A901E98F2E61
GO

Sequence cautioni

The sequence AAB01678 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAA19686 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAB78971 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_008361198 – 211LELGI…VVIGL → RTHIYTYRISLYFK in isoform 2. 1 PublicationAdd BLAST14
Alternative sequenceiVSP_008362212 – 347Missing in isoform 2. 1 PublicationAdd BLAST136

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL024486 Genomic DNA. Translation: CAA19686.1. Different initiation.
AL161551 Genomic DNA. Translation: CAB78971.1. Different initiation.
CP002687 Genomic DNA. Translation: AEE84216.1.
CP002687 Genomic DNA. Translation: AEE84217.1.
AY087095 mRNA. Translation: AAM64655.1.
U27590 mRNA. Translation: AAB01678.1. Different initiation.
PIRiT04750.
RefSeqiNP_567590.3. NM_118089.4. [Q38856-1]
NP_849546.1. NM_179215.2. [Q38856-2]
UniGeneiAt.2319.

Genome annotation databases

EnsemblPlantsiAT4G19690.2; AT4G19690.2; AT4G19690. [Q38856-1]
GeneIDi827713.
GrameneiAT4G19690.2; AT4G19690.2; AT4G19690.
KEGGiath:AT4G19690.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL024486 Genomic DNA. Translation: CAA19686.1. Different initiation.
AL161551 Genomic DNA. Translation: CAB78971.1. Different initiation.
CP002687 Genomic DNA. Translation: AEE84216.1.
CP002687 Genomic DNA. Translation: AEE84217.1.
AY087095 mRNA. Translation: AAM64655.1.
U27590 mRNA. Translation: AAB01678.1. Different initiation.
PIRiT04750.
RefSeqiNP_567590.3. NM_118089.4. [Q38856-1]
NP_849546.1. NM_179215.2. [Q38856-2]
UniGeneiAt.2319.

3D structure databases

ProteinModelPortaliQ38856.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi13006. 3 interactors.
DIPiDIP-60391N.
STRINGi3702.AT4G19690.2.

Protein family/group databases

TCDBi2.A.5.1.2. the zinc (zn(2+))-iron (fe(2+)) permease (zip) family.

Proteomic databases

PaxDbiQ38856.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G19690.2; AT4G19690.2; AT4G19690. [Q38856-1]
GeneIDi827713.
GrameneiAT4G19690.2; AT4G19690.2; AT4G19690.
KEGGiath:AT4G19690.

Organism-specific databases

TAIRiAT4G19690.

Phylogenomic databases

eggNOGiKOG1558. Eukaryota.
ENOG4111GP2. LUCA.
HOGENOMiHOG000157076.
InParanoidiQ38856.
KOiK14709.
OMAiCEHIINT.
OrthoDBiEOG09360GCY.

Enzyme and pathway databases

BioCyciARA:AT4G19690-MONOMER.
MetaCyc:AT4G19690-MONOMER.

Miscellaneous databases

PROiQ38856.

Gene expression databases

GenevisibleiQ38856. AT.

Family and domain databases

InterProiIPR003689. ZIP.
IPR004698. Zn/Fe_permease_fun/pln.
[Graphical view]
PfamiPF02535. Zip. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00820. zip. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiIRT1_ARATH
AccessioniPrimary (citable) accession number: Q38856
Secondary accession number(s): Q8LBP0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: May 31, 2011
Last modified: November 30, 2016
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Inhibited by cadmium and Fe2+ ions and at 100-fold excess inhibited by cobalt, manganese and zinc ions.
The availability of secondary non-iron metal substrates (Zn, Mn, and Co) controls the localization of IRT1 between the outer polar domain of the plasma membrane and early endosome/trans-Golgi network in root epidermal cells.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.