UniProtKB - Q38856 (IRT1_ARATH)
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Protein
Fe(2+) transport protein 1
Gene
IRT1
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
High-affinity iron transporter that plays a key role in the uptake of iron from the rhizosphere across the plasma membrane in the root epidermal layer. Acts as the principal regulator of iron homeostasis in planta. Also mediates the heavy metals uptake under iron-deficiency by its ability to transport cobalt, cadmium, manganese and/or zinc ions.1 Publication
Miscellaneous
Inhibited by cadmium and Fe2+ ions and at 100-fold excess inhibited by cobalt, manganese and zinc ions.
The availability of secondary non-iron metal substrates (Zn, Mn, and Co) controls the localization of IRT1 between the outer polar domain of the plasma membrane and early endosome/trans-Golgi network in root epidermal cells.1 Publication
Caution
GO - Molecular functioni
- iron ion transmembrane transporter activity Source: TAIR
- zinc ion transmembrane transporter activity Source: InterPro
GO - Biological processi
- cellular response to ethylene stimulus Source: TAIR
- cellular response to iron ion Source: TAIR
- cellular response to nitric oxide Source: TAIR
- iron ion homeostasis Source: UniProtKB-KW
- metal ion transport Source: TAIR
- nickel cation transport Source: TAIR
- response to bacterium Source: TAIR
Keywordsi
Biological process | Ion transport, Iron transport, Transport |
Ligand | Iron |
Enzyme and pathway databases
BioCyci | ARA:AT4G19690-MONOMER MetaCyc:AT4G19690-MONOMER |
Protein family/group databases
TCDBi | 2.A.5.1.2 the zinc (zn(2+))-iron (fe(2+)) permease (zip) family |
Names & Taxonomyi
Protein namesi | Recommended name: Fe(2+) transport protein 1Alternative name(s): Fe(II) transport protein 1 Iron-regulated transporter 1 |
Gene namesi | Name:IRT1 Ordered Locus Names:At4g19690 ORF Names:T16H5.50 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT4G19690 |
TAIRi | locus:2133965 AT4G19690 |
Subcellular locationi
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 23 – 52 | ExtracellularSequence analysisAdd BLAST | 30 | |
Transmembranei | 53 – 73 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 74 – 84 | CytoplasmicSequence analysisAdd BLAST | 11 | |
Transmembranei | 85 – 105 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 106 – 125 | ExtracellularSequence analysisAdd BLAST | 20 | |
Transmembranei | 126 – 146 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 147 – 192 | CytoplasmicSequence analysisAdd BLAST | 46 | |
Transmembranei | 193 – 213 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 214 – 224 | ExtracellularSequence analysisAdd BLAST | 11 | |
Transmembranei | 225 – 245 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 246 – 254 | CytoplasmicSequence analysis | 9 | |
Transmembranei | 255 – 275 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 276 – 286 | ExtracellularSequence analysisAdd BLAST | 11 | |
Transmembranei | 287 – 307 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 308 – 326 | CytoplasmicSequence analysisAdd BLAST | 19 | |
Transmembranei | 327 – 347 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Cell membrane, Endosome, Golgi apparatus, Membrane, VacuolePathology & Biotechi
Disruption phenotypei
Plants exhibit a lethal chlorotic phenotype.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 104 | H → A: Suppresses transport. 1 Publication | 1 | |
Mutagenesisi | 108 | D → A: Abolishes iron and manganese transport. 1 Publication | 1 | |
Mutagenesisi | 111 | E → A: Abolishes zinc transport. Abolishes iron and manganese transport; when associated with A-108. 1 Publication | 1 | |
Mutagenesisi | 144 | D → A: Abolishes iron and manganese transport. Reduces cadmium transport. 1 Publication | 1 | |
Mutagenesisi | 154 | K → R: Suppresses ubiquitination and loss of activity; when associated with R-179. 1 Publication | 1 | |
Mutagenesisi | 179 | K → R: Suppresses ubiquitination and loss of activity; when associated with R-154. 1 Publication | 1 | |
Mutagenesisi | 205 | H → A or E: Suppresses transport. 1 Publication | 1 | |
Mutagenesisi | 206 | S → A: Suppresses transport. 1 Publication | 1 | |
Mutagenesisi | 232 | H → A: Suppresses transport. 1 Publication | 1 | |
Mutagenesisi | 236 | E → A: Suppresses transport. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 22 | Sequence analysisAdd BLAST | 22 | |
ChainiPRO_0000041636 | 23 – 347 | Fe(2+) transport protein 1Add BLAST | 325 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 154 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication | ||
Cross-linki | 179 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication |
Post-translational modificationi
Monoubiquitinated on several Lys residues. Monoubiquitination controls trafficking from the plasma membrane and targeting to the vacuole.1 Publication
Keywords - PTMi
Isopeptide bond, Ubl conjugationProteomic databases
PaxDbi | Q38856 |
PTM databases
iPTMneti | Q38856 |
Expressioni
Tissue specificityi
Expressed in the external cell layers of the root including the lateral branching zone. Also detected in flowers before pollination.1 Publication
Inductioni
In roots by iron starvation.1 Publication
Gene expression databases
ExpressionAtlasi | Q38856 baseline and differential |
Genevisiblei | Q38856 AT |
Interactioni
Subunit structurei
Interacts with FREE1.1 Publication
Binary interactionsi
With | Entry | #Exp. | IntAct | Notes |
---|---|---|---|---|
UBQ16 | P59263 | 3 | EBI-15928951,EBI-1541543 |
Protein-protein interaction databases
BioGridi | 130063 interactors. |
DIPi | DIP-60391N |
IntActi | Q38856 1 interactor. |
STRINGi | 3702.AT4G19690.2 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 205 – 206 | Heavy metals binding | 2 |
Sequence similaritiesi
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1558 Eukaryota ENOG4111GP2 LUCA |
HOGENOMi | HOG000157076 |
InParanoidi | Q38856 |
KOi | K14709 |
OMAi | EMLSSIC |
OrthoDBi | EOG09360GCY |
Family and domain databases
InterProi | View protein in InterPro IPR003689 ZIP IPR004698 Zn/Fe_permease_fun/pln |
Pfami | View protein in Pfam PF02535 Zip, 1 hit |
TIGRFAMsi | TIGR00820 zip, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q38856-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
10 20 30 40 50
MASNSALLMK TIFLVLIFVS FAISPATSTA PEECGSESAN PCVNKAKALP
60 70 80 90 100
LKVIAIFVIL IASMIGVGAP LFSRNVSFLQ PDGNIFTIIK CFASGIILGT
110 120 130 140 150
GFMHVLPDSF EMLSSICLEE NPWHKFPFSG FLAMLSGLIT LAIDSMATSL
160 170 180 190 200
YTSKNAVGIM PHGHGHGHGP ANDVTLPIKE DDSSNAQLLR YRVIAMVLEL
210 220 230 240 250
GIIVHSVVIG LSLGATSDTC TIKGLIAALC FHQMFEGMGL GGCILQAEYT
260 270 280 290 300
NMKKFVMAFF FAVTTPFGIA LGIALSTVYQ DNSPKALITV GLLNACSAGL
310 320 330 340
LIYMALVDLL AAEFMGPKLQ GSIKMQFKCL IAALLGCGGM SIIAKWA
Sequence cautioni
The sequence AAB01678 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAA19686 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAB78971 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_008361 | 198 – 211 | LELGI…VVIGL → RTHIYTYRISLYFK in isoform 2. 1 PublicationAdd BLAST | 14 | |
Alternative sequenceiVSP_008362 | 212 – 347 | Missing in isoform 2. 1 PublicationAdd BLAST | 136 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL024486 Genomic DNA Translation: CAA19686.1 Different initiation. AL161551 Genomic DNA Translation: CAB78971.1 Different initiation. CP002687 Genomic DNA Translation: AEE84216.1 CP002687 Genomic DNA Translation: AEE84217.1 AY087095 mRNA Translation: AAM64655.1 U27590 mRNA Translation: AAB01678.1 Different initiation. |
PIRi | T04750 |
RefSeqi | NP_567590.3, NM_118089.4 [Q38856-1] NP_849546.1, NM_179215.2 [Q38856-2] |
UniGenei | At.2319 |
Genome annotation databases
EnsemblPlantsi | AT4G19690.1; AT4G19690.1; AT4G19690 [Q38856-2] AT4G19690.2; AT4G19690.2; AT4G19690 [Q38856-1] |
GeneIDi | 827713 |
Gramenei | AT4G19690.1; AT4G19690.1; AT4G19690 [Q38856-2] AT4G19690.2; AT4G19690.2; AT4G19690 [Q38856-1] |
KEGGi | ath:AT4G19690 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Entry informationi
Entry namei | IRT1_ARATH | |
Accessioni | Q38856Primary (citable) accession number: Q38856 Secondary accession number(s): Q8LBP0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 26, 2003 |
Last sequence update: | May 31, 2011 | |
Last modified: | April 25, 2018 | |
This is version 143 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |