Reviewed, UniProtKB/Swiss-Prot Q38854 (DXS_ARATH)

Names and origin

Protein names

Recommended name:
    Probable 1-deoxy-D-xylulose-5-phosphate synthase, chloroplastic
      Short name=1-deoxyxylulose-5-phosphate synthase
      Short name=DXP synthase
      Short name=DXPS
    EC=2.2.1.7

Gene names

Name:	CLA1
Synonyms:	DEF
Ordered Locus Names:	At4g15560
ORF Names:	dl3821w

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	717 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. Essential for chloroplast development.
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2.
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1.
Subcellular location	Plastid › chloroplast Probable.
Induction	By light.
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chlorophyll biosynthetic process Inferred from mutant phenotype. Source: TAIR isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Traceable author statement. Source: TAIR terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro thiamin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from direct assay. Source: TAIR
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from direct assay. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 58	58	Chloroplast Potential
	Chain	59 – 717	659	Probable 1-deoxy-D-xylulose-5-phosphate synthase, chloroplastic		PRO_0000007393
Amino acid modifications
	Modified residue	276	1	Phosphoserine Ref.6
Experimental info
	Sequence conflict	201	1	V → I in CAA74713. Ref.2
	Sequence conflict	301	1	L → V in CAA74713. Ref.2
	Sequence conflict	307	1	E → V Ref.1
	Sequence conflict	307	1	E → V Ref.2
	Sequence conflict	397	1	K → E Ref.1
	Sequence conflict	397	1	K → E Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q38854-1 [UniParc]. Last modified February 21, 2001. Version 2. Checksum: 005A5E5585116A4F	FASTA	717	76,833
	10 20 30 40 50 60 MASSAFAFPS YIITKGGLST DSCKSTSLSS SRSLVTDLPS PCLKPNNNSH SNRRAKVCAS 70 80 90 100 110 120 LAEKGEYYSN RPPTPLLDTI NYPIHMKNLS VKELKQLSDE LRSDVIFNVS KTGGHLGSSL 130 140 150 160 170 180 GVVELTVALH YIFNTPQDKI LWDVGHQSYP HKILTGRRGK MPTMRQTNGL SGFTKRGESE 190 200 210 220 230 240 HDCFGTGHSS TTISAGLGMA VGRDLKGKNN NVVAVIGDGA MTAGQAYEAM NNAGYLDSDM 250 260 270 280 290 300 IVILNDNKQV SLPTATLDGP SPPVGALSSA LSRLQSNPAL RELREVAKGM TKQIGGPMHQ 310 320 330 340 350 360 LAAKVDEYAR GMISGTGSSL FEELGLYYIG PVDGHNIDDL VAILKEVKST RTTGPVLIHV 370 380 390 400 410 420 VTEKGRGYPY AERADDKYHG VVKFDPATGR QFKTTNKTQS YTTYFAEALV AEAEVDKDVV 430 440 450 460 470 480 AIHAAMGGGT GLNLFQRRFP TRCFDVGIAE QHAVTFAAGL ACEGLKPFCA IYSSFMQRAY 490 500 510 520 530 540 DQVVHDVDLQ KLPVRFAMDR AGLVGADGPT HCGAFDVTFM ACLPNMIVMA PSDEADLFNM 550 560 570 580 590 600 VATAVAIDDR PSCFRYPRGN GIGVALPPGN KGVPIEIGKG RILKEGERVA LLGYGSAVQS 610 620 630 640 650 660 CLGAAVMLEE RGLNVTVADA RFCKPLDRAL IRSLAKSHEV LITVEEGSIG GFGSHVVQFL 670 680 690 700 710 ALDGLLDGKL KWRPMVLPDR YIDHGAPADQ LAEAGLMPSH IAATALNLIG APREALF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"CLA1, a novel gene required for chloroplast development, is highly conserved in evolution." Mandel M.A., Feldmann K.A., Herrera-Estrella L., Rocha-Sosa M., Leon P. Plant J. 9:649-658(1996) [PubMed: 8653115] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	Camara B. Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana." Bevan M., Bancroft I., Bent E., Love K., Goodman H.M., Dean C., Bergkamp R., Dirkse W., van Staveren M., Stiekema W., Drost L., Ridley P., Hudson S.-A., Patel K., Murphy G., Piffanelli P., Wedler H., Wedler E. , Wambutt R., Weitzenegger T., Pohl T., Terryn N., Gielen J., Villarroel R., De Clercq R., van Montagu M., Lecharny A., Aubourg S., Gy I., Kreis M., Lao N., Kavanagh T., Hempel S., Kotter P., Entian K.-D., Rieger M., Schaefer M., Funk B., Mueller-Auer S., Silvey M., James R., Monfort A., Pons A., Puigdomenech P., Douka A., Voukelatou E., Milioni D., Hatzopoulos P., Piravandi E., Obermaier B., Hilbert H., Duesterhoeft A., Moores T., Jones J.D.G., Eneva T., Palme K., Benes V., Rechmann S., Ansorge W., Cooke R., Berger C., Delseny M., Voet M., Volckaert G., Mewes H.-W., Klosterman S., Schueller C., Chalwatzis N. Nature 391:485-488(1998) [PubMed: 9461215] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks." Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S. Plant Physiol. 150:889-903(2009) [PubMed: 19376835] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-276, MASS SPECTROMETRY. Tissue: Seedling.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U27099 mRNA. Translation: AAC49368.1. Y14333 mRNA. Translation: CAA74713.1. Different initiation. Z97339 Genomic DNA. Translation: CAB45992.1. AL161542 Genomic DNA. Translation: CAB78598.1. BT002340 mRNA. Translation: AAN86173.1.
IPI	IPI00516684.
PIR	H85171. T52289.
RefSeq	NP_193291.1.
UniGene	At.23240 At.66955
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q38854.
Proteomic databases
PRIDE	Q38854.
Genome annotation databases
GeneID	827230.
GenomeReviews	Gene locus AT4G15560 in contig CT486007_GR.
KEGG	ath:AT4G15560.
NMPDR	fig|3702.1.peg.19305.
Organism-specific databases
TAIR	At4g15560.
Phylogenomic databases
OMA	RRFPTRC.
Enzyme and pathway databases
BioCyc	MetaCyc:AT4G15560-MON.
BRENDA	2.2.1.7. 302.
Gene expression databases
Genevestigator	Q38854.
GermOnline	AT4G15560. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR005477. Dxylulose-5-P_synthase. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
Pfam	PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00204. dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_ARATH

Accession

Primary (citable) accession number: Q38854
Secondary accession number(s): O23407, O49738

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	February 21, 2001
Last modified:	November 3, 2009
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)