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Protein

Ethylene response sensor 1

Gene

ERS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ethylene receptor related to bacterial two-component regulators. Acts as a redundant negative regulator of ethylene signaling.

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

Cofactori

Cu cationBy similarityNote: Binds 1 copper ion per dimer.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi65CopperBy similarity1
Metal bindingi69CopperBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ethylene binding Source: TAIR
  • metal ion binding Source: UniProtKB-KW
  • phosphorelay sensor kinase activity Source: InterPro
  • protein histidine kinase activity Source: TAIR
  • protein serine/threonine kinase activity Source: UniProtKB-KW
  • receptor activity Source: TAIR

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Receptor, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Ethylene signaling pathway, Two-component regulatory system

Keywords - Ligandi

ATP-binding, Copper, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ethylene response sensor 1 (EC:2.7.11.-, EC:2.7.13.3)
Short name:
AtERS1
Alternative name(s):
Protein ERS1
Gene namesi
Name:ERS1
Synonyms:ERS
Ordered Locus Names:At2g40940
ORF Names:T20B5.14
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G40940.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei23 – 43HelicalSequence analysisAdd BLAST21
Transmembranei58 – 78HelicalSequence analysisAdd BLAST21
Transmembranei95 – 115HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype in ethylene response; due to the redundancy with ETR1. Ers1 and etr1 double mutants display a constitutive ethylene-response phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003781421 – 613Ethylene response sensor 1Add BLAST613

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi4InterchainBy similarity
Disulfide bondi6InterchainBy similarity
Modified residuei353Phosphohistidine; by autocatalysisPROSITE-ProRule annotationBy similarity1

Post-translational modificationi

Autophosphorylated on both His and Ser residues in the presence of manganese. Loss of His autophosphorylation in the presence of both manganese and magnesium.1 Publication

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiQ38846.

Expressioni

Tissue specificityi

Expressed in etiolated seedlings, leaves, stems, roots, flowers, embryos, anthers, carpels and ovules.1 Publication

Inductioni

By ethylene.2 Publications

Gene expression databases

GenevisibleiQ38846. AT.

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Heteromer with ETR1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CTR1Q056092EBI-1606754,EBI-1606697
TRP1Q8L7L82EBI-1606754,EBI-476071

Protein-protein interaction databases

BioGridi4030. 5 interactors.
IntActiQ38846. 5 interactors.
STRINGi3702.AT2G40940.1.

Structurei

Secondary structure

1613
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi309 – 370Combined sources62
Helixi376 – 403Combined sources28

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4MT8X-ray1.90A/B308-407[»]
4MTXX-ray2.15A/B/C/D308-407[»]
ProteinModelPortaliQ38846.
SMRiQ38846.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini158 – 307GAFAdd BLAST150
Domaini350 – 589Histidine kinasePROSITE-ProRule annotationAdd BLAST240

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi592 – 597Poly-Ser6

Sequence similaritiesi

Belongs to the ethylene receptor family.Curated
Contains 1 GAF domain.Curated
Contains 1 histidine kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IRCC. Eukaryota.
ENOG410ZCWX. LUCA.
HOGENOMiHOG000240343.
InParanoidiQ38846.
KOiK14509.
OMAiMHSKAVA.
OrthoDBiEOG093605FG.
PhylomeDBiQ38846.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.450.40. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003018. GAF.
IPR029016. GAF_dom-like.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF01590. GAF. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00065. GAF. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55781. SSF55781. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q38846-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESCDCFETH VNQDDLLVKY QYISDALIAL AYFSIPLELI YFVQKSAFFP
60 70 80 90 100
YKWVLMQFGA FIILCGATHF INLWMFFMHS KAVAIVMTIA KVSCAVVSCA
110 120 130 140 150
TALMLVHIIP DLLSVKNREL FLKKKADELD REMGLILTQE ETGRHVRMLT
160 170 180 190 200
HGIRRTLDRH TILRTTLVEL GKTLCLEECA LWMPSQSGLY LQLSHTLSHK
210 220 230 240 250
IQVGSSVPIN LPIINELFNS AQAMHIPHSC PLAKIGPPVG RYSPPEVVSV
260 270 280 290 300
RVPLLHLSNF QGSDWSDLSG KGYAIMVLIL PTDGARKWRD HELELVENVA
310 320 330 340 350
DQVAVALSHA AILEESMHAR DQLMEQNFAL DKARQEAEMA VHARNDFLAV
360 370 380 390 400
MNHEMRTPMH AIISLSSLLL ETELSPEQRV MIETILKSSN LVATLISDVL
410 420 430 440 450
DLSRLEDGSL LLENEPFSLQ AIFEEVISLI KPIASVKKLS TNLILSADLP
460 470 480 490 500
TYAIGDEKRL MQTILNIMGN AVKFTKEGYI SIIASIMKPE SLQELPSPEF
510 520 530 540 550
FPVLSDSHFY LCVQVKDTGC GIHTQDIPLL FTKFVQPRTG TQRNHSGGGL
560 570 580 590 600
GLALCKRFVG LMGGYMWIES EGLEKGCTAS FIIRLGICNG PSSSSGSMAL
610
HLAAKSQTRP WNW
Length:613
Mass (Da):68,333
Last modified:November 1, 1996 - v1
Checksum:iE4F5DB384E6727B2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U21952 Genomic DNA. Translation: AAC49090.1.
AC002409 Genomic DNA. Translation: AAB86454.1.
CP002685 Genomic DNA. Translation: AEC09904.1.
AY054532 mRNA. Translation: AAK96723.1.
BT000159 mRNA. Translation: AAN15478.1.
PIRiT00758.
RefSeqiNP_181626.1. NM_129658.4.
UniGeneiAt.21783.
At.66415.

Genome annotation databases

EnsemblPlantsiAT2G40940.1; AT2G40940.1; AT2G40940.
GeneIDi818693.
GrameneiAT2G40940.1; AT2G40940.1; AT2G40940.
KEGGiath:AT2G40940.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U21952 Genomic DNA. Translation: AAC49090.1.
AC002409 Genomic DNA. Translation: AAB86454.1.
CP002685 Genomic DNA. Translation: AEC09904.1.
AY054532 mRNA. Translation: AAK96723.1.
BT000159 mRNA. Translation: AAN15478.1.
PIRiT00758.
RefSeqiNP_181626.1. NM_129658.4.
UniGeneiAt.21783.
At.66415.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4MT8X-ray1.90A/B308-407[»]
4MTXX-ray2.15A/B/C/D308-407[»]
ProteinModelPortaliQ38846.
SMRiQ38846.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4030. 5 interactors.
IntActiQ38846. 5 interactors.
STRINGi3702.AT2G40940.1.

Proteomic databases

PaxDbiQ38846.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G40940.1; AT2G40940.1; AT2G40940.
GeneIDi818693.
GrameneiAT2G40940.1; AT2G40940.1; AT2G40940.
KEGGiath:AT2G40940.

Organism-specific databases

TAIRiAT2G40940.

Phylogenomic databases

eggNOGiENOG410IRCC. Eukaryota.
ENOG410ZCWX. LUCA.
HOGENOMiHOG000240343.
InParanoidiQ38846.
KOiK14509.
OMAiMHSKAVA.
OrthoDBiEOG093605FG.
PhylomeDBiQ38846.

Miscellaneous databases

PROiQ38846.

Gene expression databases

GenevisibleiQ38846. AT.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.450.40. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003018. GAF.
IPR029016. GAF_dom-like.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF01590. GAF. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00065. GAF. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55781. SSF55781. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiERS1_ARATH
AccessioniPrimary (citable) accession number: Q38846
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.