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Protein

Ethylene response sensor 1

Gene

ERS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ethylene receptor related to bacterial two-component regulators. Acts as a redundant negative regulator of ethylene signaling.

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

Cofactori

Cu cationBy similarityNote: Binds 1 copper ion per dimer.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi65 – 651CopperBy similarity
Metal bindingi69 – 691CopperBy similarity

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ethylene binding Source: TAIR
  • metal ion binding Source: UniProtKB-KW
  • phosphorelay sensor kinase activity Source: InterPro
  • protein histidine kinase activity Source: TAIR
  • protein serine/threonine kinase activity Source: UniProtKB-KW
  • receptor activity Source: TAIR

GO - Biological processi

  • ethylene-activated signaling pathway Source: UniProtKB-KW
  • negative regulation of ethylene-activated signaling pathway Source: TAIR
  • peptidyl-histidine phosphorylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Receptor, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Ethylene signaling pathway, Two-component regulatory system

Keywords - Ligandi

ATP-binding, Copper, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G40940-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ethylene response sensor 1 (EC:2.7.11.-, EC:2.7.13.3)
Short name:
AtERS1
Alternative name(s):
Protein ERS1
Gene namesi
Name:ERS1
Synonyms:ERS
Ordered Locus Names:At2g40940
ORF Names:T20B5.14
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G40940.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei23 – 4321HelicalSequence analysisAdd
BLAST
Transmembranei58 – 7821HelicalSequence analysisAdd
BLAST
Transmembranei95 – 11521HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype in ethylene response; due to the redundancy with ETR1. Ers1 and etr1 double mutants display a constitutive ethylene-response phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 613613Ethylene response sensor 1PRO_0000378142Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi4 – 4InterchainBy similarity
Disulfide bondi6 – 6InterchainBy similarity
Modified residuei353 – 3531Phosphohistidine; by autocatalysisPROSITE-ProRule annotationBy similarity

Post-translational modificationi

Autophosphorylated on both His and Ser residues in the presence of manganese. Loss of His autophosphorylation in the presence of both manganese and magnesium.1 Publication

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiQ38846.
PRIDEiQ38846.

Expressioni

Tissue specificityi

Expressed in etiolated seedlings, leaves, stems, roots, flowers, embryos, anthers, carpels and ovules.1 Publication

Inductioni

By ethylene.2 Publications

Gene expression databases

GenevisibleiQ38846. AT.

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Heteromer with ETR1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CTR1Q056092EBI-1606754,EBI-1606697
TRP1Q8L7L82EBI-1606754,EBI-476071

Protein-protein interaction databases

BioGridi4030. 5 interactions.
IntActiQ38846. 5 interactions.
STRINGi3702.AT2G40940.1.

Structurei

Secondary structure

1
613
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi309 – 37062Combined sources
Helixi376 – 40328Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4MT8X-ray1.90A/B308-407[»]
4MTXX-ray2.15A/B/C/D308-407[»]
ProteinModelPortaliQ38846.
SMRiQ38846. Positions 308-587.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini158 – 307150GAFAdd
BLAST
Domaini350 – 589240Histidine kinasePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi592 – 5976Poly-Ser

Sequence similaritiesi

Belongs to the ethylene receptor family.Curated
Contains 1 GAF domain.Curated
Contains 1 histidine kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IRCC. Eukaryota.
ENOG410ZCWX. LUCA.
HOGENOMiHOG000240343.
InParanoidiQ38846.
KOiK14509.
OMAiMHSKAVA.
PhylomeDBiQ38846.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.450.40. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003018. GAF.
IPR029016. GAF_dom-like.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF01590. GAF. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00065. GAF. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55781. SSF55781. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q38846-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESCDCFETH VNQDDLLVKY QYISDALIAL AYFSIPLELI YFVQKSAFFP
60 70 80 90 100
YKWVLMQFGA FIILCGATHF INLWMFFMHS KAVAIVMTIA KVSCAVVSCA
110 120 130 140 150
TALMLVHIIP DLLSVKNREL FLKKKADELD REMGLILTQE ETGRHVRMLT
160 170 180 190 200
HGIRRTLDRH TILRTTLVEL GKTLCLEECA LWMPSQSGLY LQLSHTLSHK
210 220 230 240 250
IQVGSSVPIN LPIINELFNS AQAMHIPHSC PLAKIGPPVG RYSPPEVVSV
260 270 280 290 300
RVPLLHLSNF QGSDWSDLSG KGYAIMVLIL PTDGARKWRD HELELVENVA
310 320 330 340 350
DQVAVALSHA AILEESMHAR DQLMEQNFAL DKARQEAEMA VHARNDFLAV
360 370 380 390 400
MNHEMRTPMH AIISLSSLLL ETELSPEQRV MIETILKSSN LVATLISDVL
410 420 430 440 450
DLSRLEDGSL LLENEPFSLQ AIFEEVISLI KPIASVKKLS TNLILSADLP
460 470 480 490 500
TYAIGDEKRL MQTILNIMGN AVKFTKEGYI SIIASIMKPE SLQELPSPEF
510 520 530 540 550
FPVLSDSHFY LCVQVKDTGC GIHTQDIPLL FTKFVQPRTG TQRNHSGGGL
560 570 580 590 600
GLALCKRFVG LMGGYMWIES EGLEKGCTAS FIIRLGICNG PSSSSGSMAL
610
HLAAKSQTRP WNW
Length:613
Mass (Da):68,333
Last modified:November 1, 1996 - v1
Checksum:iE4F5DB384E6727B2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U21952 Genomic DNA. Translation: AAC49090.1.
AC002409 Genomic DNA. Translation: AAB86454.1.
CP002685 Genomic DNA. Translation: AEC09904.1.
AY054532 mRNA. Translation: AAK96723.1.
BT000159 mRNA. Translation: AAN15478.1.
PIRiT00758.
RefSeqiNP_181626.1. NM_129658.3.
UniGeneiAt.21783.
At.66415.

Genome annotation databases

EnsemblPlantsiAT2G40940.1; AT2G40940.1; AT2G40940.
GeneIDi818693.
GrameneiAT2G40940.1; AT2G40940.1; AT2G40940.
KEGGiath:AT2G40940.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U21952 Genomic DNA. Translation: AAC49090.1.
AC002409 Genomic DNA. Translation: AAB86454.1.
CP002685 Genomic DNA. Translation: AEC09904.1.
AY054532 mRNA. Translation: AAK96723.1.
BT000159 mRNA. Translation: AAN15478.1.
PIRiT00758.
RefSeqiNP_181626.1. NM_129658.3.
UniGeneiAt.21783.
At.66415.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4MT8X-ray1.90A/B308-407[»]
4MTXX-ray2.15A/B/C/D308-407[»]
ProteinModelPortaliQ38846.
SMRiQ38846. Positions 308-587.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4030. 5 interactions.
IntActiQ38846. 5 interactions.
STRINGi3702.AT2G40940.1.

Proteomic databases

PaxDbiQ38846.
PRIDEiQ38846.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G40940.1; AT2G40940.1; AT2G40940.
GeneIDi818693.
GrameneiAT2G40940.1; AT2G40940.1; AT2G40940.
KEGGiath:AT2G40940.

Organism-specific databases

TAIRiAT2G40940.

Phylogenomic databases

eggNOGiENOG410IRCC. Eukaryota.
ENOG410ZCWX. LUCA.
HOGENOMiHOG000240343.
InParanoidiQ38846.
KOiK14509.
OMAiMHSKAVA.
PhylomeDBiQ38846.

Enzyme and pathway databases

BioCyciARA:AT2G40940-MONOMER.

Miscellaneous databases

PROiQ38846.

Gene expression databases

GenevisibleiQ38846. AT.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.450.40. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003018. GAF.
IPR029016. GAF_dom-like.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF01590. GAF. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00065. GAF. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55781. SSF55781. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Ethylene insensitivity conferred by Arabidopsis ERS gene."
    Hua J., Chang C., Sun Q., Meyerowitz E.M.
    Science 269:1712-1714(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Columbia.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "EIN4 and ERS2 are members of the putative ethylene receptor gene family in Arabidopsis."
    Hua J., Sakai H., Nourizadeh S., Chen Q.G., Bleecker A.B., Ecker J.R., Meyerowitz E.M.
    Plant Cell 10:1321-1332(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, INDUCTION BY ETHYLENE.
  6. Cited for: DISULFIDE BONDS, SUBCELLULAR LOCATION.
  7. "Autophosphorylation activity of the Arabidopsis ethylene receptor multigene family."
    Moussatche P., Klee H.J.
    J. Biol. Chem. 279:48734-48741(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION.
  8. "A strong constitutive ethylene-response phenotype conferred on Arabidopsis plants containing null mutations in the ethylene receptors ETR1 and ERS1."
    Qu X., Hall B.P., Gao Z., Schaller G.E.
    BMC Plant Biol. 7:3-3(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  9. "Heteromeric interactions among ethylene receptors mediate signaling in Arabidopsis."
    Gao Z., Wen C.-K., Binder B.M., Chen Y.-F., Chang J., Chiang Y.-H., Kerris R.J. III, Chang C., Schaller G.E.
    J. Biol. Chem. 283:23801-23810(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ETR1, INDUCTION BY ETHYLENE.

Entry informationi

Entry nameiERS1_ARATH
AccessioniPrimary (citable) accession number: Q38846
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: November 1, 1996
Last modified: February 17, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.