Q38830 (IAA12_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Auxin-responsive protein IAA12

Alternative name(s):
Indoleacetic acid-induced protein 12
Protein BODENLOS

Gene names

Name:	IAA12
Synonyms:	BDL
Ordered Locus Names:	At1g04550
ORF Names:	T1G11.20

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	239 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Aux/IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations. Repression is thought to result from the interaction with auxin response factors (ARFs), proteins that bind to the auxin-responsive promoter element (AuxRE). Formation of heterodimers with ARF proteins may alter their ability to modulate early auxin response genes expression. Ref.7
Subunit structure	Homo- and heterodimers. Interacts with the auxin response factor ARF5. Interacts (via domain I) with TPL (via the LisH domain). Ref.6 Ref.9
Subcellular location	Nucleus.
Tissue specificity	Preferentially expressed in stems, leaves and flowers. Ref.1
Induction	By auxin. Ref.1
Domain	The N-terminal half of the protein contains two conserved domains I and II. Domain I includes a slightly degenerated ERF-associated amphiphilic repression (EAR) motif which seems to be involved in the activity of transcriptional repression. Domain II is required for the correct degradation of the protein through the SCF-mediated ubiquitin-proteasome pathway. Interactions between Aux/IAA proteins and auxin response factors (ARFs) occur through their C-terminal dimerization domains III and IV. Ref.8
Sequence similarities	Belongs to the Aux/IAA family. Contains 1 Aux/IAA-ARF domain.

Ontologies

Keywords
Biological process	Auxin signaling pathway Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Molecular function	Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	auxin mediated signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW embryonic pattern specification Inferred from mutant phenotype. Source: TAIR negative regulation of transcription, DNA-dependent Inferred from direct assay Ref.9. Source: TAIR root development Inferred from mutant phenotype Ref.9. Source: TAIR transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW xylem and phloem pattern formation Inferred from mutant phenotype Ref.9. Source: TAIR
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein dimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ARF5	P93024	3	EBI-617608,EBI-629519

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q38830-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 239	239	Auxin-responsive protein IAA12		PRO_0000112843
Regions
	Domain	125 – 218	94	Aux/IAA-ARF
	Motif	20 – 24	5	EAR-like (transcriptional repression)
Experimental info
	Mutagenesis	20	1	L → A: Decreased interaction with TPL; when associated with A-22 and A-24. Ref.9
	Mutagenesis	22	1	L → A: Decreased interaction with TPL; when associated with A-20 and A-24. Ref.9
	Mutagenesis	24	1	L → A: Decreased interaction with TPL; when associated with A-20 and A-22. Ref.9
	Mutagenesis	74	1	P → S in bdl; gain of function. Affects auxin-related developmental processes.
	Sequence conflict	119	1	P → A in AAM65588. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: BBAADA3E98B222CA	FASTA	239	26,286
	10 20 30 40 50 60 MRGVSELEVG KSNLPAESEL ELGLGLSLGG GAWKERGRIL TAKDFPSVGS KRSAESSSHQ 70 80 90 100 110 120 GASPPRSSQV VGWPPIGLHR MNSLVNNQAM KAARAEEGDG EKKVVKNDEL KDVSMKVNPK 130 140 150 160 170 180 VQGLGFVKVN MDGVGIGRKV DMRAHSSYEN LAQTLEEMFF GMTGTTCREK VKPLRLLDGS 190 200 210 220 230 SDFVLTYEDK EGDWMLVGDV PWRMFINSVK RLRIMGTSEA SGLAPRRQEQ KDRQRNNPV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The PS-IAA4/5-like family of early auxin-inducible mRNAs in Arabidopsis thaliana." Abel S., Nguyen M.D., Theologis A. J. Mol. Biol. 251:533-549(1995) [PubMed: 7658471] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"The Arabidopsis BODENLOS gene encodes an auxin response protein inhibiting MONOPTEROS-mediated embryo patterning." Hamann T., Benkova E., Baeurle I., Kientz M., Juergens G. Genes Dev. 16:1610-1615(2002) [PubMed: 12101120] [Abstract] Cited for: MUTANT BDL, INTERACTION WITH ARF5.
[7]	"Genetics of Aux/IAA and ARF action in plant growth and development." Liscum E., Reed J.W. Plant Mol. Biol. 49:387-400(2002) [PubMed: 12036262] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE, FUNCTION.
[8]	"Aux/IAA proteins contain a potent transcriptional repression domain." Tiwari S.B., Hagen G., Guilfoyle T.J. Plant Cell 16:533-543(2004) [PubMed: 14742873] [Abstract] Cited for: TRANSCRIPTIONAL REPRESSION DOMAIN.
[9]	"TOPLESS mediates auxin-dependent transcriptional repression during Arabidopsis embryogenesis." Szemenyei H., Hannon M., Long J.A. Science 319:1384-1386(2008) [PubMed: 18258861] [Abstract] Cited for: INTERACTION WITH TPL, MUTAGENESIS OF LEU-20; LEU-22 AND LEU-24.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18414 mRNA. Translation: AAC49053.1. AC002376 Genomic DNA. Translation: AAB80631.1. CP002684 Genomic DNA. Translation: AEE27713.1. AF332398 mRNA. Translation: AAG48762.1. AY065240 mRNA. Translation: AAL38716.1. AY096535 mRNA. Translation: AAM20185.1. AY088042 mRNA. Translation: AAM65588.1.
IPI	IPI00527949.
PIR	S58498.
RefSeq	NP_171949.1. NM_100334.3.
UniGene	At.10466. At.10797.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q38830. 29 interactions.
STRING	Q38830.
Proteomic databases
PRIDE	Q38830.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G04550.2; AT1G04550.2; AT1G04550.
GeneID	839495.
GenomeReviews	Gene locus AT1G04550 in contig CT485782_GR.
KEGG	ath:AT1G04550.
NMPDR	fig|3702.1.peg.601.
Organism-specific databases
GeneFarm	3159. 342.
TAIR	At1g04550.
Phylogenomic databases
GeneTree	EPGT00050000005298.
HOGENOM	HBG750482.
InParanoid	Q38830.
OMA	DFVLTYE.
PhylomeDB	Q38830.
ProtClustDB	CLSN2681806.
Gene expression databases
ArrayExpress	Q38830.
Genevestigator	Q38830.
Family and domain databases
InterPro	IPR003311. AUX_IAA. IPR011525. AuxIAA_ARF_dimer. [Graphical view]
KO	K14484.
Pfam	PF02309. AUX_IAA. 1 hit. [Graphical view]
PROSITE	PS50962. IAA_ARF. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IAA12_ARATH

Accession

Primary (citable) accession number: Q38830
Secondary accession number(s): Q8LA42

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program