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Protein

Auxin-responsive protein IAA12

Gene

IAA12

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Aux/IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations. Repression is thought to result from the interaction with auxin response factors (ARFs), proteins that bind to the auxin-responsive promoter element (AuxRE). Formation of heterodimers with ARF proteins may alter their ability to modulate early auxin response genes expression.1 Publication

GO - Molecular functioni

  • DNA binding transcription factor activity Source: TAIR

GO - Biological processi

  • auxin-activated signaling pathway Source: UniProtKB-KW
  • embryonic pattern specification Source: TAIR
  • negative regulation of transcription, DNA-templated Source: TAIR
  • root development Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW
  • xylem and phloem pattern formation Source: TAIR

Keywordsi

Molecular functionRepressor
Biological processAuxin signaling pathway, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Auxin-responsive protein IAA12
Alternative name(s):
Indoleacetic acid-induced protein 12
Protein BODENLOS
Gene namesi
Name:IAA12
Synonyms:BDL
Ordered Locus Names:At1g04550
ORF Names:T1G11.20
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G04550
TAIRilocus:2197838 AT1G04550

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi20L → A: Decreased interaction with TPL; when associated with A-22 and A-24. 1 Publication1
Mutagenesisi22L → A: Decreased interaction with TPL; when associated with A-20 and A-24. 1 Publication1
Mutagenesisi24L → A: Decreased interaction with TPL; when associated with A-20 and A-22. 1 Publication1
Mutagenesisi74P → S in bdl; gain of function. Affects auxin-related developmental processes. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001128431 – 239Auxin-responsive protein IAA12Add BLAST239

Proteomic databases

PaxDbiQ38830
PRIDEiQ38830

PTM databases

iPTMnetiQ38830

Expressioni

Tissue specificityi

Preferentially expressed in stems, leaves and flowers.1 Publication

Inductioni

By auxin.1 Publication

Gene expression databases

ExpressionAtlasiQ38830 baseline and differential
GenevisibleiQ38830 AT

Interactioni

Subunit structurei

Homodimers and heterodimers. Interacts with the auxin response factor ARF5. Interacts (via domain I) with TPL (via the LisH domain).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ARF5P930244EBI-617608,EBI-629519

Protein-protein interaction databases

BioGridi24730, 33 interactors
ELMiQ38830
IntActiQ38830, 27 interactors
STRINGi3702.AT1G04550.2

Structurei

3D structure databases

ProteinModelPortaliQ38830
SMRiQ38830
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini124 – 217PB1PROSITE-ProRule annotationAdd BLAST94

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi20 – 24EAR-like (transcriptional repression)5

Domaini

The N-terminal half of the protein contains two conserved domains I and II. Domain I includes a slightly degenerated ERF-associated amphiphilic repression (EAR) motif which seems to be involved in the activity of transcriptional repression. Domain II is required for the correct degradation of the protein through the SCF-mediated ubiquitin-proteasome pathway. Interactions between Aux/IAA proteins and auxin response factors (ARFs) occur through their C-terminal dimerization domains III and IV.

Sequence similaritiesi

Belongs to the Aux/IAA family.Curated

Phylogenomic databases

eggNOGiENOG410IVBZ Eukaryota
ENOG410YK71 LUCA
HOGENOMiHOG000238261
InParanoidiQ38830
KOiK14484
OMAiYQGIHRT
OrthoDBiEOG09360LM2
PhylomeDBiQ38830

Family and domain databases

InterProiView protein in InterPro
IPR033389 AUX/IAA_dom
IPR003311 AUX_IAA
IPR000270 PB1_dom
PANTHERiPTHR31734 PTHR31734, 1 hit
PfamiView protein in Pfam
PF02309 AUX_IAA, 1 hit
PROSITEiView protein in PROSITE
PS51745 PB1, 1 hit

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q38830-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRGVSELEVG KSNLPAESEL ELGLGLSLGG GAWKERGRIL TAKDFPSVGS
60 70 80 90 100
KRSAESSSHQ GASPPRSSQV VGWPPIGLHR MNSLVNNQAM KAARAEEGDG
110 120 130 140 150
EKKVVKNDEL KDVSMKVNPK VQGLGFVKVN MDGVGIGRKV DMRAHSSYEN
160 170 180 190 200
LAQTLEEMFF GMTGTTCREK VKPLRLLDGS SDFVLTYEDK EGDWMLVGDV
210 220 230
PWRMFINSVK RLRIMGTSEA SGLAPRRQEQ KDRQRNNPV
Length:239
Mass (Da):26,286
Last modified:November 1, 1996 - v1
Checksum:iBBAADA3E98B222CA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti119P → A in AAM65588 (Ref. 5) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18414 mRNA Translation: AAC49053.1
AC002376 Genomic DNA Translation: AAB80631.1
CP002684 Genomic DNA Translation: AEE27713.1
AF332398 mRNA Translation: AAG48762.1
AY065240 mRNA Translation: AAL38716.1
AY096535 mRNA Translation: AAM20185.1
AY088042 mRNA Translation: AAM65588.1
PIRiS58498
RefSeqiNP_171949.1, NM_100334.4 [Q38830-1]
UniGeneiAt.10466
At.10797

Genome annotation databases

EnsemblPlantsiAT1G04550.2; AT1G04550.2; AT1G04550 [Q38830-1]
GeneIDi839495
GrameneiAT1G04550.2; AT1G04550.2; AT1G04550 [Q38830-1]
KEGGiath:AT1G04550

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiIAA12_ARATH
AccessioniPrimary (citable) accession number: Q38830
Secondary accession number(s): Q8LA42
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: April 25, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health