Auxin-responsive protein IAA7 - Arabidopsis thaliana (Mouse-ear cress)

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Q38825 (IAA7_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 99. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Auxin-responsive protein IAA7

Alternative name(s):
Auxin resistant 2
Indoleacetic acid-induced protein 7

Gene names

Name:	IAA7
Synonyms:	AXR2
Ordered Locus Names:	At3g23050
ORF Names:	MXC7.8

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	243 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Aux/IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations. Repression is thought to result from the interaction with auxin response factors (ARFs), proteins that bind to the auxin-responsive promoter element (AuxRE). Formation of heterodimers with ARF proteins may alter their ability to modulate early auxin response genes expression. Ref.7
Subunit structure	Homo- and heterodimers By similarity.
Subcellular location	Nucleus By similarity.
Tissue specificity	Highly expressed in stems and flowers. Ref.1
Induction	By auxin. Ref.1
Domain	The N-terminal half of the protein contains two conserved domains I and II. Domain I includes a slightly degenerated ERF-associated amphiphilic repression (EAR) motif which seems to be involved in the activity of transcriptional repression. Domain II is required for the correct degradation of the protein through the SCF-mediated ubiquitin-proteasome pathway. Interactions between Aux/IAA proteins and auxin response factors (ARFs) occur through their C-terminal dimerization domains III and IV. Ref.8
Sequence similarities	Belongs to the Aux/IAA family. Contains 1 Aux/IAA-ARF domain.
Sequence caution	The sequence AAM65301.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Auxin signaling pathway Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Molecular function	Repressor
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	auxin mediated signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW gravitropism Inferred from mutant phenotype. Source: TAIR regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW response to jasmonic acid stimulus Inferred from expression pattern. Source: TAIR response to water deprivation Inferred from mutant phenotype. Source: TAIR response to wounding Inferred from expression pattern. Source: TAIR transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from direct assay. Source: TAIR
Molecular function	protein dimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
CUL1	Q94AH6	5	EBI-602959,EBI-532411
SKP1A	Q39255	2	EBI-602959,EBI-532357
TIR1	Q570C0	4	EBI-602959,EBI-307183

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select] Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q38825-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 243

243

Auxin-responsive protein IAA7

PRO_0000112838

Regions

Domain

125 – 225

101

Aux/IAA-ARF

Motif

13 – 17

EAR-like (transcriptional repression)

Experimental info

Mutagenesis

P → S in axr2-1; gain of function. Affects auxin-related developmental processes. Affects photomorphogenesis.

Sequence conflict

228

V → I in AAM65301. Ref.5

Sequences

Sequence

Length

Mass (Da)

Tools

Isoform 1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 830E9B9EA2C2B5D0
Show »

FASTA

243

26,381

        10         20         30         40         50         60 
MIGQLMNLKA TELCLGLPGG AEAVESPAKS AVGSKRGFSE TVDLMLNLQS NKEGSVDLKN 

        70         80         90        100        110        120 
VSAVPKEKTT LKDPSKPPAK AQVVGWPPVR NYRKNMMTQQ KTSSGAEEAS SEKAGNFGGG 

       130        140        150        160        170        180 
AAGAGLVKVS MDGAPYLRKV DLKMYKSYQD LSDALAKMFS SFTMGNYGAQ GMIDFMNESK 

       190        200        210        220        230        240 
LMNLLNSSEY VPSYEDKDGD WMLVGDVPWE MFVESCKRLR IMKGSEAVGL APRAMEKYCK 


NRS

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The PS-IAA4/5-like family of early auxin-inducible mRNAs in Arabidopsis thaliana." Abel S., Nguyen M.D., Theologis A. J. Mol. Biol. 251:533-549(1995) [PubMed: 7658471] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION. Strain: cv. Columbia.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S. DNA Res. 7:131-135(2000) [PubMed: 10819329] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"AXR2 encodes a member of the Aux/IAA protein family." Nagpal P., Walker L.M., Young J.C., Sonawala A., Timpte C., Estelle M., Reed J.W. Plant Physiol. 123:563-574(2000) [PubMed: 10859186] [Abstract] Cited for: MUTANT AXR2-1.
[7]	"Genetics of Aux/IAA and ARF action in plant growth and development." Liscum E., Reed J.W. Plant Mol. Biol. 49:387-400(2002) [PubMed: 12036262] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE, FUNCTION.
[8]	"Aux/IAA proteins contain a potent transcriptional repression domain." Tiwari S.B., Hagen G., Guilfoyle T.J. Plant Cell 16:533-543(2004) [PubMed: 14742873] [Abstract] Cited for: TRANSCRIPTIONAL REPRESSION DOMAIN.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U18409 mRNA. Translation: AAC49048.1.
AB026655 Genomic DNA. Translation: BAB02096.1.
CP002686 Genomic DNA. Translation: AEE76710.1.
AF332395 mRNA. Translation: AAG48759.1.
AY054651 mRNA. Translation: AAK96842.1.
AY072461 mRNA. Translation: AAL66876.1.
AY087765 mRNA. Translation: AAM65301.1. Different initiation.

IPI

IPI00517741.

PIR

S58494.

RefSeq

NP_188945.1. NM_113205.2.

UniGene

At.22260.
At.67783.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2P1N	X-ray	2.50	C/F	82-94	[»]
2P1O	X-ray	1.90	C	82-94	[»]
2P1Q	X-ray	1.91	C	82-94	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-33345N.

IntAct

Q38825. 37 interactions.

STRING

Q38825.

Proteomic databases

PRIDE

Q38825.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblPlants

AT3G23050.1; AT3G23050.1; AT3G23050.

GeneID

821879.

GenomeReviews

Gene locus AT3G23050 in contig BA000014_GR.

KEGG

ath:AT3G23050.

NMPDR

fig|3702.1.peg.14555.

Organism-specific databases

GeneFarm

3154. 342.

TAIR

At3g23050.

Phylogenomic databases

HOGENOM

HBG750482.

InParanoid

Q38825.

OMA

NESKLMN.

PhylomeDB

Q38825.

ProtClustDB

CLSN2684190.

Gene expression databases

ArrayExpress

Q38825.

Genevestigator

Q38825.

Family and domain databases

InterPro

IPR003311. AUX_IAA.
IPR011525. AuxIAA_ARF_dimer.
[Graphical view]

K14484.

Pfam

PF02309. AUX_IAA. 1 hit.
[Graphical view]

PROSITE

PS50962. IAA_ARF. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

IAA7_ARATH

Accession

Primary (citable) accession number: Q38825
Secondary accession number(s): Q8LAK1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents