Reviewed, UniProtKB/Swiss-Prot Q38825 (IAA7_ARATH)

Names and origin

Protein names

Recommended name:
    Auxin-responsive protein IAA7
Alternative name(s):
    Indoleacetic acid-induced protein 7
    Auxin resistant 2

Gene names

Name:	IAA7
Synonyms:	AXR2
Ordered Locus Names:	At3g23050
ORF Names:	MXC7.8

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	243 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Aux/IAA proteins are short-lived transcriptional factors that function as repressor of early auxin response genes at low auxin concentrations. Repression is thought to result from the interaction with auxin response factors (ARFs), proteins that bind to the auxin-responsive promoter element (AuxRE). Formation of heterodimers with ARF proteins may alter their ability to modulate early auxin response genes expression.
Subunit structure	Homo and heterodimers By similarity.
Subcellular location	NucleusBy similarity.
Tissue specificity	Highly expressed in stems and flowers.
Induction	By auxin.
Domain	The N-terminal half of the protein contains two conserved domains I and II. Domain I includes a slightly degenerated ERF-associated amphiphilic repression (EAR) motif which seems to be involved in the activity of transcriptional repression. Domain II is required for the correct degradation of the protein through the SCF-mediated ubiquitin-proteasome pathway. Interactions between Aux/IAA proteins and auxin response factors (ARFs) occur through their C-terminal dimerization domains III and IV.
Sequence similarities	Belongs to the Aux/IAA family. Contains 1 Aux/IAA-ARF domain.

Ontologies

Keywords
Biological process	Auxin signaling pathway Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Molecular function	Repressor
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
AFB2	Q9LW29	1	EBI-602959,EBI-617556
AFB3	Q9LPW7	1	EBI-602959,EBI-617501
CUL1	Q94AH6	3	EBI-602959,EBI-532411
GRH1	Q9ZR12	1	EBI-602959,EBI-617479
SKP1A	Q39255	2	EBI-602959,EBI-532357
TIR1	Q570C0	3	EBI-602959,EBI-307183

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Notes: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q38825-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 243	243	Auxin-responsive protein IAA7
Regions
	Domain	125 – 225	101	Aux/IAA-ARF
	Motif	13 – 17	5	EAR-like (transcriptional repression)
Experimental info
	Mutagenesis	87	1	P → S in axr2-1; gain of function. Affects auxin-related developmental processes. Affects photomorphogenesis
	Sequence conflict	228	1	V → I in AAM65301. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 830E9B9EA2C2B5D0	FASTA	243	26,381
	10 20 30 40 50 60 MIGQLMNLKA TELCLGLPGG AEAVESPAKS AVGSKRGFSE TVDLMLNLQS NKEGSVDLKN 70 80 90 100 110 120 VSAVPKEKTT LKDPSKPPAK AQVVGWPPVR NYRKNMMTQQ KTSSGAEEAS SEKAGNFGGG 130 140 150 160 170 180 AAGAGLVKVS MDGAPYLRKV DLKMYKSYQD LSDALAKMFS SFTMGNYGAQ GMIDFMNESK 190 200 210 220 230 240 LMNLLNSSEY VPSYEDKDGD WMLVGDVPWE MFVESCKRLR IMKGSEAVGL APRAMEKYCK NRS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The PS-IAA4/5-like family of early auxin-inducible mRNAs in Arabidopsis thaliana." Abel S., Nguyen M.D., Theologis A. J. Mol. Biol. 251:533-549(1995) [PubMed: 7658471] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION. Strain: cv. Columbia.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S. DNA Res. 7:131-135(2000) [PubMed: 10819329] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"AXR2 encodes a member of the Aux/IAA protein family." Nagpal P., Walker L.M., Young J.C., Sonawala A., Timpte C., Estelle M., Reed J.W. Plant Physiol. 123:563-574(2000) [PubMed: 10859186] [Abstract] Cited for: MUTANT AXR2-1.
[6]	"Genetics of Aux/IAA and ARF action in plant growth and development." Liscum E., Reed J.W. Plant Mol. Biol. 49:387-400(2002) [PubMed: 12036262] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE, FUNCTION.
[7]	"Aux/IAA proteins contain a potent transcriptional repression domain." Tiwari S.B., Hagen G., Guilfoyle T.J. Plant Cell 16:533-543(2004) [PubMed: 14742873] [Abstract] Cited for: TRANSCRIPTIONAL REPRESSION DOMAIN.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U18409 mRNA. Translation: AAC49048.1. AB026655 Genomic DNA. Translation: BAB02096.1. AF332395 mRNA. Translation: AAG48759.1. AY054651 mRNA. Translation: AAK96842.1. AY072461 mRNA. Translation: AAL66876.1. AY087765 mRNA. Translation: AAM65301.1. Different initiation.
PIR	S58494.
RefSeq	NP_188945.1.
UniGene	At.22260
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q38825.
Genome annotation databases
GeneID	821879.
GenomeReviews	Gene locus AT3G23050 in contig BA000014_GR.
KEGG	ath:AT3G23050.
NMPDR	fig|3702.1.peg.14555.
Organism-specific databases
GeneFarm	3154. 342.
TAIR	At3g23050.
Gene expression databases
ArrayExpress	Q38825.
Family and domain databases
InterPro	IPR003311. AUX_IAA. IPR011525. AuxIAA_ARF_dimer. [Graphical view]
Pfam	PF02309. AUX_IAA. 1 hit. [Graphical view]
PROSITE	PS50962. IAA_ARF. 1 hit. [Graphical view]
ProDom	Q38825. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

IAA7_ARATH

Accession

Primary (citable) accession number: Q38825
Secondary accession number(s): Q8LAK1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1996
Last modified:	July 22, 2008
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)