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Protein

Auxin-responsive protein IAA3

Gene

IAA3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Aux/IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations. Repression is thought to result from the interaction with auxin response factors (ARFs), proteins that bind to the auxin-responsive promoter element (AuxRE). Plays a central role in auxin regulation of root growth, in gravitropism, and in lateral root formation (PubMed:9895319). Regulated by an auxin-induced protein turnover (PubMed:14617065). Formation of heterodimers with ARF proteins may alter their ability to modulate early auxin response genes expression. When activated by cytokinin, restricts the expression of the PIN genes to the vascular transition zone (PubMed:19039136). Induction of SHY2 in the vascular transition zone restricts BRX expression to down-regulate PIN3 and thus limit meristem growth, but proper SHY2 expression requires BRX (PubMed:21149702). Involved in meristem growth and in determining its size (PubMed:20605455). May participate in strigolactone signaling to regulate meristem size and lateral root formation (PubMed:23425316).8 Publications

GO - Molecular functioni

  • transcription factor activity, sequence-specific DNA binding Source: TAIR
  • transcription regulatory region DNA binding Source: TAIR

GO - Biological processi

  • auxin-activated signaling pathway Source: UniProtKB-KW
  • response to auxin Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionRepressor
Biological processAuxin signaling pathway, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Auxin-responsive protein IAA3
Alternative name(s):
Indoleacetic acid-induced protein 3
Short hypocotyl
Suppressor of HY2
Gene namesi
Name:IAA3
Synonyms:SHY2
Ordered Locus Names:At1g04240
ORF Names:F19P19.32
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRilocus:2018379. AT1G04240.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi67G → E in shy2-3; gain of function. Affects auxin-related developmental processes. Affects photomorphogenesis. 1 Publication1
Mutagenesisi69P → S in shy2-2; gain of function. Affects auxin-related developmental processes. Affects photomorphogenesis. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001128341 – 189Auxin-responsive protein IAA3Add BLAST189

Post-translational modificationi

Phosphorylated by phytochrome A in vitro.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ38822.

Expressioni

Tissue specificityi

Highly expressed in stems and flowers (PubMed:7658471). Expressed in hypocotyls, cotyledons and leaves, but barely detected in roots (PubMed:11884676). Expressed in root tips (PubMed:14534134). In the root meristem, specifically detected at the vascular tissue transition zone (PubMed:19039136).4 Publications

Developmental stagei

The level of expression increases during meristem growth, reaches a maximum at 5 days post germination and subsequently remaines constant in time.1 Publication

Inductioni

By auxin (PubMed:7658471). Up-regulated by cytokinin treatment through the primary cytokinin-response transcription factor ARR1 (PubMed:19039136). Down-regulated by light in the presence of sucrose, but up-regulated in the absence of sucrose (PubMed:11884676).3 Publications

Gene expression databases

ExpressionAtlasiQ38822. baseline and differential.
GenevisibleiQ38822. AT.

Interactioni

Subunit structurei

Homodimers and heterodimers (By similarity). Interacts with TPL (PubMed:18258861). Interacts with TIR1, the F-box component of the Skp1-Cdc53/cullin-F-box (SCFTIR1) E3 ubiquitin ligase complex (PubMed:14617065).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
IAA1P496773EBI-307174,EBI-630505

Protein-protein interaction databases

BioGridi24805. 42 interactors.
DIPiDIP-31741N.
IntActiQ38822. 35 interactors.
STRINGi3702.AT1G04240.1.

Structurei

3D structure databases

ProteinModelPortaliQ38822.
SMRiQ38822.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini92 – 179PB1PROSITE-ProRule annotationAdd BLAST88

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi12 – 16EAR-like (transcriptional repression)5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi33 – 36Poly-Asn4

Domaini

The N-terminal half of the protein contains two conserved domains I and II. Domain I includes a slightly degenerated ERF-associated amphiphilic repression (EAR) motif which seems to be involved in the activity of transcriptional repression. Domain II is required for the correct degradation of the protein through the SCF-mediated ubiquitin-proteasome pathway. Interactions between Aux/IAA proteins and auxin response factors (ARFs) occur through their C-terminal dimerization domains III and IV.

Sequence similaritiesi

Belongs to the Aux/IAA family.Curated

Phylogenomic databases

eggNOGiENOG410IX07. Eukaryota.
ENOG410YIDV. LUCA.
HOGENOMiHOG000238261.
InParanoidiQ38822.
KOiK14484.
OMAiDLSCYKG.
OrthoDBiEOG09360PSB.
PhylomeDBiQ38822.

Family and domain databases

InterProiView protein in InterPro
IPR033389. AUX/IAA_dom.
IPR003311. AUX_IAA.
IPR000270. PB1_dom.
PANTHERiPTHR31734. PTHR31734. 1 hit.
PfamiView protein in Pfam
PF02309. AUX_IAA. 1 hit.
PROSITEiView protein in PROSITE
PS51745. PB1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q38822-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDEFVNLKET ELRLGLPGTD NVCEAKERVS CCNNNNKRVL STDTEKEIES
60 70 80 90 100
SSRKTETSPP RKAQIVGWPP VRSYRKNNIQ SKKNESEHEG QGIYVKVSMD
110 120 130 140 150
GAPYLRKIDL SCYKGYSELL KALEVMFKFS VGEYFERDGY KGSDFVPTYE
160 170 180
DKDGDWMLIG DVPWEMFICT CKRLRIMKGS EAKGLGCGV
Length:189
Mass (Da):21,520
Last modified:November 1, 1996 - v1
Checksum:i8627A4173363EF27
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18406 mRNA. Translation: AAC49045.1.
AC000104 Genomic DNA. Translation: AAB70452.1.
CP002684 Genomic DNA. Translation: AEE27673.1.
AF332393 mRNA. Translation: AAG48757.1.
AY064007 mRNA. Translation: AAL36363.1.
PIRiS58491.
RefSeqiNP_171920.1. NM_100305.4.
UniGeneiAt.20801.

Genome annotation databases

EnsemblPlantsiAT1G04240.1; AT1G04240.1; AT1G04240.
GeneIDi839570.
GrameneiAT1G04240.1; AT1G04240.1; AT1G04240.
KEGGiath:AT1G04240.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18406 mRNA. Translation: AAC49045.1.
AC000104 Genomic DNA. Translation: AAB70452.1.
CP002684 Genomic DNA. Translation: AEE27673.1.
AF332393 mRNA. Translation: AAG48757.1.
AY064007 mRNA. Translation: AAL36363.1.
PIRiS58491.
RefSeqiNP_171920.1. NM_100305.4.
UniGeneiAt.20801.

3D structure databases

ProteinModelPortaliQ38822.
SMRiQ38822.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi24805. 42 interactors.
DIPiDIP-31741N.
IntActiQ38822. 35 interactors.
STRINGi3702.AT1G04240.1.

Proteomic databases

PaxDbiQ38822.

Protocols and materials databases

DNASUi839570.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G04240.1; AT1G04240.1; AT1G04240.
GeneIDi839570.
GrameneiAT1G04240.1; AT1G04240.1; AT1G04240.
KEGGiath:AT1G04240.

Organism-specific databases

AraportiAT1G04240.
TAIRilocus:2018379. AT1G04240.

Phylogenomic databases

eggNOGiENOG410IX07. Eukaryota.
ENOG410YIDV. LUCA.
HOGENOMiHOG000238261.
InParanoidiQ38822.
KOiK14484.
OMAiDLSCYKG.
OrthoDBiEOG09360PSB.
PhylomeDBiQ38822.

Miscellaneous databases

PROiQ38822.

Gene expression databases

ExpressionAtlasiQ38822. baseline and differential.
GenevisibleiQ38822. AT.

Family and domain databases

InterProiView protein in InterPro
IPR033389. AUX/IAA_dom.
IPR003311. AUX_IAA.
IPR000270. PB1_dom.
PANTHERiPTHR31734. PTHR31734. 1 hit.
PfamiView protein in Pfam
PF02309. AUX_IAA. 1 hit.
PROSITEiView protein in PROSITE
PS51745. PB1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiIAA3_ARATH
AccessioniPrimary (citable) accession number: Q38822
Secondary accession number(s): O04741
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: February 15, 2017
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.