Q38822 (IAA3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Auxin-responsive protein IAA3

Alternative name(s):
Indoleacetic acid-induced protein 3
Short hypocotyl
Suppressor of HY2

Gene names

Name:	IAA3
Synonyms:	SHY2
Ordered Locus Names:	At1g04240
ORF Names:	F19P19.32

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	189 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Aux/IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations. Repression is thought to result from the interaction with auxin response factors (ARFs), proteins that bind to the auxin-responsive promoter element (AuxRE). Formation of heterodimers with ARF proteins may alter their ability to modulate early auxin response genes expression. Ref.7
Subunit structure	Homo- and heterodimers By similarity. Interacts with TPL. Ref.9
Subcellular location	Nucleus By similarity.
Tissue specificity	Highly expressed in stems and flowers. Ref.1
Induction	By auxin. Ref.1
Domain	The N-terminal half of the protein contains two conserved domains I and II. Domain I includes a slightly degenerated ERF-associated amphiphilic repression (EAR) motif which seems to be involved in the activity of transcriptional repression. Domain II is required for the correct degradation of the protein through the SCF-mediated ubiquitin-proteasome pathway. Interactions between Aux/IAA proteins and auxin response factors (ARFs) occur through their C-terminal dimerization domains III and IV. Ref.8
Post-translational modification	Phosphorylated by phytochrome A in vitro. Ref.6
Sequence similarities	Belongs to the Aux/IAA family. Contains 1 Aux/IAA-ARF domain.

Ontologies

Keywords
Biological process	Auxin signaling pathway Transcription Transcription regulation
Cellular component	Nucleus
Molecular function	Repressor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	auxin mediated signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein dimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
IAA1	P49677	3	EBI-307174,EBI-630505

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 189	189	Auxin-responsive protein IAA3		PRO_0000112834
Regions
	Domain	93 – 180	88	Aux/IAA-ARF
	Motif	12 – 16	5	EAR-like (transcriptional repression)
	Compositional bias	33 – 36	4	Poly-Asn
Experimental info
	Mutagenesis	67	1	G → E in shy2-3; gain of function. Affects auxin-related developmental processes. Affects photomorphogenesis.
	Mutagenesis	69	1	P → S in shy2-2; gain of function. Affects auxin-related developmental processes. Affects photomorphogenesis.

Sequences

	Sequence		Length	Mass (Da)
	Q38822 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 8627A4173363EF27	FASTA	189	21,520
	10 20 30 40 50 60 MDEFVNLKET ELRLGLPGTD NVCEAKERVS CCNNNNKRVL STDTEKEIES SSRKTETSPP 70 80 90 100 110 120 RKAQIVGWPP VRSYRKNNIQ SKKNESEHEG QGIYVKVSMD GAPYLRKIDL SCYKGYSELL 130 140 150 160 170 180 KALEVMFKFS VGEYFERDGY KGSDFVPTYE DKDGDWMLIG DVPWEMFICT CKRLRIMKGS EAKGLGCGV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The PS-IAA4/5-like family of early auxin-inducible mRNAs in Arabidopsis thaliana." Abel S., Nguyen M.D., Theologis A. J. Mol. Biol. 251:533-549(1995) [PubMed: 7658471] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Control of auxin-regulated root development by the Arabidopsis thaliana SHY2/IAA3 gene." Tian Q., Reed J.W. Development 126:711-721(1999) [PubMed: 9895319] [Abstract] Cited for: MUTANTS SHY2-2 AND SHY2-3.
[6]	"Aux/IAA proteins are phosphorylated by phytochrome in vitro." Colon-Carmona A., Chen D.L., Yeh K.-C., Abel S. Plant Physiol. 124:1728-1738(2000) [PubMed: 11115889] [Abstract] Cited for: PHOSPHORYLATION BY PHYTOCHROME A.
[7]	"Genetics of Aux/IAA and ARF action in plant growth and development." Liscum E., Reed J.W. Plant Mol. Biol. 49:387-400(2002) [PubMed: 12036262] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE, FUNCTION.
[8]	"Aux/IAA proteins contain a potent transcriptional repression domain." Tiwari S.B., Hagen G., Guilfoyle T.J. Plant Cell 16:533-543(2004) [PubMed: 14742873] [Abstract] Cited for: TRANSCRIPTIONAL REPRESSION DOMAIN.
[9]	"TOPLESS mediates auxin-dependent transcriptional repression during Arabidopsis embryogenesis." Szemenyei H., Hannon M., Long J.A. Science 319:1384-1386(2008) [PubMed: 18258861] [Abstract] Cited for: INTERACTION WITH TPL.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18406 mRNA. Translation: AAC49045.1. AC000104 Genomic DNA. Translation: AAB70452.1. CP002684 Genomic DNA. Translation: AEE27673.1. AF332393 mRNA. Translation: AAG48757.1. AY064007 mRNA. Translation: AAL36363.1.
IPI	IPI00519738.
PIR	S58491.
RefSeq	NP_171920.1. NM_100305.3.
UniGene	At.20801.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-31741N.
IntAct	Q38822. 37 interactions.
STRING	Q38822.
Proteomic databases
ProMEX	Q38822.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G04240.1; AT1G04240.1; AT1G04240.
GeneID	839570.
GenomeReviews	Gene locus AT1G04240 in contig CT485782_GR.
KEGG	ath:AT1G04240.
NMPDR	fig|3702.1.peg.570.
Organism-specific databases
GeneFarm	3143. 342.
TAIR	At1g04240.
Phylogenomic databases
GeneTree	EPGT00050000005298.
HOGENOM	HBG750482.
InParanoid	Q38822.
OMA	KNESEHE.
PhylomeDB	Q38822.
ProtClustDB	CLSN2681778.
Gene expression databases
ArrayExpress	Q38822.
Genevestigator	Q38822.
GermOnline	AT1G04240. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR003311. AUX_IAA. IPR011525. AuxIAA_ARF_dimer. [Graphical view]
KO	K14484.
Pfam	PF02309. AUX_IAA. 1 hit. [Graphical view]
PROSITE	PS50962. IAA_ARF. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IAA3_ARATH

Accession

Primary (citable) accession number: Q38822
Secondary accession number(s): O04741

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program