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Protein

Thiamine thiazole synthase, chloroplastic

Gene

THI1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in biosynthesis of the thiamine precursor thiazole. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an adenylated thiazole intermediate. The reaction includes an iron-dependent sulfide transfer from a conserved cysteine residue of the protein to a thiazole intermediate. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme. May have additional roles in adaptation to various stress conditions and in DNA damage tolerance.UniRule annotation2 Publications

Cofactori

Fe cationUniRule annotationNote: Binds 1 Fe cation per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei94 – 941Substrate; via amide nitrogen
Binding sitei122 – 1221Substrate; via amide nitrogen
Binding sitei187 – 1871Substrate; via amide nitrogen and carbonyl oxygen
Binding sitei218 – 2181Substrate
Binding sitei233 – 2331Substrate
Binding sitei285 – 2851Substrate; via amide nitrogen

GO - Molecular functioni

  • oxidoreductase activity Source: InterPro
  • protein homodimerization activity Source: TAIR
  • zinc ion binding Source: TAIR

GO - Biological processi

  • cellular response to DNA damage stimulus Source: TAIR
  • oxazole or thiazole biosynthetic process Source: TAIR
  • response to cold Source: TAIR
  • thiamine biosynthetic process Source: TAIR
  • thiazole biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

Iron, Metal-binding, NAD

Enzyme and pathway databases

BioCyciMetaCyc:AT5G54770-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine thiazole synthase, chloroplasticUniRule annotation
Alternative name(s):
Thiazole biosynthetic enzymeUniRule annotation
Gene namesi
Name:THI1UniRule annotation
Synonyms:ARA6, THI4
Ordered Locus Names:At5g54770
ORF Names:MBG8.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G54770.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast envelope Source: TAIR
  • chloroplast stroma Source: TAIR
  • cytosol Source: UniProtKB-HAMAP
  • mitochondrion Source: TAIR
  • stromule Source: TAIR
  • thylakoid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Mitochondrion, Plastid

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi1 – 11M → I: No chloroplastic isoform produced. 1 Publication
Mutagenesisi70 – 701M → I: No mitochondrial isoform produced. 1 Publication
Mutagenesisi77 – 771D → G: No effect. 1 Publication
Mutagenesisi85 – 851D → G: No effect. 1 Publication
Mutagenesisi121 – 1211G → V: Disrupts thiamine biosynthesis. 1 Publication
Mutagenesisi124 – 1241W → L: No effect. 1 Publication
Mutagenesisi172 – 1721K → M: No effect. 1 Publication
Mutagenesisi184 – 1841A → V in tz-201; disrupts thiamine biosynthesis. 2 Publications
Mutagenesisi211 – 2111H → F: Disrupts thiamine biosynthesis and DNA damage tolerance activity. 1 Publication
Mutagenesisi266 – 2661E → G: Disrupts thiamine biosynthesis. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5555ChloroplastAdd
BLAST
Chaini56 – 349294Thiamine thiazole synthase, chloroplasticPRO_0000034060Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei216 – 21612,3-didehydroalanine (Cys)

Post-translational modificationi

During the catalytic reaction, a sulfide is transferred from Cys-216 to a reaction intermediate, generating a dehydroalanine residue.

Proteomic databases

PaxDbiQ38814.
PRIDEiQ38814.

PTM databases

iPTMnetiQ38814.

Expressioni

Tissue specificityi

Expressed at high levels in chloroplast-containing parenchymatic cells of leaves, inflorescence shoots and flowers, and at lower levels in the vascular system. In young plants, detected in roots and shoots including cotyledons, leaves and hypocotyls. Also observed in apical meristematic regions, siliques and embryos. Low expression in roots, limited to the vascular tissue.3 Publications

Developmental stagei

Expressed throughout develpoment.1 Publication

Inductioni

Up-regulated by osmotic and salt stresses and by abscisic acid treatment. No effect of thiamine, salicylic acid or paraquat treatments. Down-regulated by dark incubation.3 Publications

Gene expression databases

GenevisibleiQ38814. AT.

Interactioni

Subunit structurei

Homooctamer.UniRule annotation1 Publication

GO - Molecular functioni

  • protein homodimerization activity Source: TAIR

Protein-protein interaction databases

BioGridi20811. 6 interactions.
IntActiQ38814. 1 interaction.
MINTiMINT-8071157.
STRINGi3702.AT5G54770.1.

Structurei

Secondary structure

1
349
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi63 – 8119Combined sources
Beta strandi83 – 897Combined sources
Helixi93 – 10311Combined sources
Beta strandi110 – 1189Combined sources
Turni121 – 1244Combined sources
Beta strandi133 – 1364Combined sources
Turni137 – 1393Combined sources
Helixi140 – 1467Combined sources
Beta strandi154 – 1607Combined sources
Helixi162 – 17413Combined sources
Beta strandi179 – 1835Combined sources
Beta strandi185 – 1939Combined sources
Beta strandi196 – 2049Combined sources
Helixi205 – 2084Combined sources
Turni211 – 2133Combined sources
Beta strandi220 – 22910Combined sources
Beta strandi233 – 2353Combined sources
Turni236 – 2394Combined sources
Helixi240 – 2478Combined sources
Beta strandi250 – 2523Combined sources
Beta strandi258 – 2603Combined sources
Helixi262 – 27211Combined sources
Beta strandi274 – 2774Combined sources
Beta strandi280 – 2823Combined sources
Helixi285 – 2917Combined sources
Helixi301 – 31717Combined sources
Turni323 – 3264Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1RP0X-ray1.60A/B49-328[»]
ProteinModelPortaliQ38814.
SMRiQ38814. Positions 51-328.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ38814.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni114 – 1152Substrate binding
Regioni295 – 2973Substrate binding

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi86 – 894Poly-Val

Sequence similaritiesi

Belongs to the THI4 family.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2960. Eukaryota.
COG1635. LUCA.
HOGENOMiHOG000106048.
InParanoidiQ38814.
KOiK03146.
OMAiRMGPVFG.
OrthoDBiEOG09360HDE.
PhylomeDBiQ38814.

Family and domain databases

Gene3Di3.50.50.60. 1 hit.
HAMAPiMF_03158. THI4. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR027495. Sti35.
IPR002922. Thi4_fam.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00292. TIGR00292. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform chloroplastic (identifier: Q38814-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAIASTLSL SSTKPQRLFD SSFHGSAISA APISIGLKPR SFSVRATTAG
60 70 80 90 100
YDLNAFTFDP IKESIVSREM TRRYMTDMIT YAETDVVVVG AGSAGLSAAY
110 120 130 140 150
EISKNPNVQV AIIEQSVSPG GGAWLGGQLF SAMIVRKPAH LFLDEIGVAY
160 170 180 190 200
DEQDTYVVVK HAALFTSTIM SKLLARPNVK LFNAVAAEDL IVKGNRVGGV
210 220 230 240 250
VTNWALVAQN HHTQSCMDPN VMEAKIVVSS CGHDGPFGAT GVKRLKSIGM
260 270 280 290 300
IDHVPGMKAL DMNTAEDAIV RLTREVVPGM IVTGMEVAEI DGAPRMGPTF
310 320 330 340
GAMMISGQKA GQLALKALGL PNAIDGTLVG NLSPELVLAA ADSAETVDA
Length:349
Mass (Da):36,664
Last modified:November 1, 1996 - v1
Checksum:iF872AB061A23CB22
GO
Isoform mitochondrial (identifier: Q38814-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-69: Missing.

Note: Mitochondrial precursor. Contains a mitochondrial presequence-like structure at its N-terminus.
Show »
Length:280
Mass (Da):29,362
Checksum:iF7CBEDD149301E40
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 6969Missing in isoform mitochondrial. CuratedVSP_044533Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17589 mRNA. Translation: AAC97124.1.
AB005232 Genomic DNA. Translation: BAB08756.1.
CP002688 Genomic DNA. Translation: AED96539.1.
AF419604 mRNA. Translation: AAL31936.1.
AF428355 mRNA. Translation: AAL16285.1.
AF428385 mRNA. Translation: AAL16153.1.
AY054216 mRNA. Translation: AAL06876.1.
AY058094 mRNA. Translation: AAL24202.1.
AY143082 mRNA. Translation: AAN12914.1.
PIRiS71191.
RefSeqiNP_200288.1. NM_124858.3. [Q38814-1]
UniGeneiAt.24888.

Genome annotation databases

EnsemblPlantsiAT5G54770.1; AT5G54770.1; AT5G54770. [Q38814-1]
GeneIDi835567.
KEGGiath:AT5G54770.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17589 mRNA. Translation: AAC97124.1.
AB005232 Genomic DNA. Translation: BAB08756.1.
CP002688 Genomic DNA. Translation: AED96539.1.
AF419604 mRNA. Translation: AAL31936.1.
AF428355 mRNA. Translation: AAL16285.1.
AF428385 mRNA. Translation: AAL16153.1.
AY054216 mRNA. Translation: AAL06876.1.
AY058094 mRNA. Translation: AAL24202.1.
AY143082 mRNA. Translation: AAN12914.1.
PIRiS71191.
RefSeqiNP_200288.1. NM_124858.3. [Q38814-1]
UniGeneiAt.24888.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1RP0X-ray1.60A/B49-328[»]
ProteinModelPortaliQ38814.
SMRiQ38814. Positions 51-328.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi20811. 6 interactions.
IntActiQ38814. 1 interaction.
MINTiMINT-8071157.
STRINGi3702.AT5G54770.1.

PTM databases

iPTMnetiQ38814.

Proteomic databases

PaxDbiQ38814.
PRIDEiQ38814.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G54770.1; AT5G54770.1; AT5G54770. [Q38814-1]
GeneIDi835567.
KEGGiath:AT5G54770.

Organism-specific databases

TAIRiAT5G54770.

Phylogenomic databases

eggNOGiKOG2960. Eukaryota.
COG1635. LUCA.
HOGENOMiHOG000106048.
InParanoidiQ38814.
KOiK03146.
OMAiRMGPVFG.
OrthoDBiEOG09360HDE.
PhylomeDBiQ38814.

Enzyme and pathway databases

BioCyciMetaCyc:AT5G54770-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ38814.
PROiQ38814.

Gene expression databases

GenevisibleiQ38814. AT.

Family and domain databases

Gene3Di3.50.50.60. 1 hit.
HAMAPiMF_03158. THI4. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR027495. Sti35.
IPR002922. Thi4_fam.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00292. TIGR00292. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTHI4_ARATH
AccessioniPrimary (citable) accession number: Q38814
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.