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Protein

Ent-copalyl diphosphate synthase, chloroplastic

Gene

GA1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of geranylgeranyl diphosphate to the gibberellin precursor ent-copalyl diphosphate.2 Publications

Catalytic activityi

Geranylgeranyl diphosphate = ent-copalyl diphosphate.

Cofactori

Mg2+1 Publication

Enzyme regulationi

Inhibited by high concentrations of magnesium.1 Publication

Pathwayi: gibberellin biosynthesis

This protein is involved in the pathway gibberellin biosynthesis, which is part of Plant hormone biosynthesis.
View all proteins of this organism that are known to be involved in the pathway gibberellin biosynthesis and in Plant hormone biosynthesis.

GO - Molecular functioni

  • ent-copalyl diphosphate synthase activity Source: TAIR
  • magnesium ion binding Source: TAIR
  • terpene synthase activity Source: InterPro

GO - Biological processi

  • gibberellic acid mediated signaling pathway Source: TAIR
  • gibberellin biosynthetic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciARA:AT4G02780-MONOMER.
MetaCyc:AT4G02780-MONOMER.
UniPathwayiUPA00390.

Names & Taxonomyi

Protein namesi
Recommended name:
Ent-copalyl diphosphate synthase, chloroplastic (EC:5.5.1.13)
Short name:
AtCPS
Short name:
Ent-CDP synthase
Alternative name(s):
Ent-kaurene synthase A
Short name:
KSA
Protein GA REQUIRING 1
Gene namesi
Name:GA1
Synonyms:ABC33, CPS, CPS1, TPSGA1
Ordered Locus Names:At4g02780
ORF Names:T5J8.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G02780.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi331 – 3311H → A: Reduced activity, but no effect on substrate binding. Increased inhibition by Mg(2+). 1 Publication
Mutagenesisi331 – 3311H → R: Reduced catalytic activity, but loss of inhibition by Mg(2+). 1 Publication
Mutagenesisi377 – 3771D → A: Reduced catalytic activity, but no effect on substrate binding. Reduced inhibition by Mg(2+). 1 Publication
Mutagenesisi379 – 3791D → A: Loss of function. 1 Publication
Mutagenesisi380 – 3801D → A: Strongly reduced activity. Reduced inhibition by Mg(2+). 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 6060ChloroplastSequence analysisAdd
BLAST
Chaini61 – 802742Ent-copalyl diphosphate synthase, chloroplasticPRO_0000033623Add
BLAST

Post-translational modificationi

The N-terminus is blocked.

Proteomic databases

PaxDbiQ38802.
PRIDEiQ38802.

Expressioni

Tissue specificityi

Expressed in roots, leaves, flowers and also in siliques.1 Publication

Gene expression databases

GenevisibleiQ38802. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G02780.1.

Structurei

Secondary structure

1
802
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi92 – 10615Combined sources
Helixi117 – 1248Combined sources
Beta strandi129 – 1346Combined sources
Helixi136 – 14510Combined sources
Beta strandi155 – 1573Combined sources
Helixi160 – 17617Combined sources
Helixi181 – 19414Combined sources
Helixi195 – 1973Combined sources
Helixi203 – 2053Combined sources
Helixi210 – 22314Combined sources
Turni232 – 2343Combined sources
Helixi235 – 24814Combined sources
Helixi251 – 2544Combined sources
Helixi260 – 2678Combined sources
Helixi273 – 2764Combined sources
Helixi277 – 2793Combined sources
Beta strandi284 – 2863Combined sources
Helixi290 – 30011Combined sources
Helixi303 – 31513Combined sources
Turni316 – 3183Combined sources
Beta strandi322 – 3243Combined sources
Helixi327 – 34014Combined sources
Helixi344 – 3474Combined sources
Helixi348 – 36114Combined sources
Beta strandi369 – 3724Combined sources
Helixi378 – 39013Combined sources
Helixi397 – 4037Combined sources
Helixi420 – 43011Combined sources
Helixi438 – 45619Combined sources
Beta strandi463 – 4664Combined sources
Helixi470 – 47910Combined sources
Helixi482 – 4843Combined sources
Helixi487 – 49711Combined sources
Helixi500 – 5023Combined sources
Beta strandi504 – 5118Combined sources
Turni514 – 5163Combined sources
Helixi519 – 54931Combined sources
Helixi552 – 5554Combined sources
Helixi559 – 57012Combined sources
Helixi576 – 5783Combined sources
Helixi579 – 59921Combined sources
Helixi603 – 61513Combined sources
Helixi640 – 66425Combined sources
Helixi669 – 68517Combined sources
Beta strandi686 – 6883Combined sources
Helixi691 – 70111Combined sources
Beta strandi703 – 7053Combined sources
Helixi708 – 7125Combined sources
Helixi714 – 72613Combined sources
Helixi742 – 76019Combined sources
Turni764 – 7663Combined sources
Helixi767 – 78620Combined sources
Helixi791 – 7999Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3PYAX-ray2.25A85-802[»]
3PYBX-ray2.76A/B/C85-802[»]
4LIXX-ray1.55A85-802[»]
ProteinModelPortaliQ38802.
SMRiQ38802. Positions 91-802.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ38802.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi377 – 3804DXDD motif

Domaini

The Asp-Xaa-Asp-Asp (DXDD) motif is important for the catalytic activity through binding to Mg2+.

Sequence similaritiesi

Belongs to the terpene synthase family. Tpsc subfamily.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IHWJ. Eukaryota.
ENOG410XNXR. LUCA.
HOGENOMiHOG000241020.
InParanoidiQ38802.
KOiK04120.
OMAiKWIIMKD.
OrthoDBiEOG093603SF.
PhylomeDBiQ38802.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
1.50.10.20. 1 hit.
1.50.30.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR001906. Terpene_synth_N.
IPR005630. Terpene_synthase_metal-bd.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF01397. Terpene_synth. 1 hit.
PF03936. Terpene_synth_C. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 3 hits.
SSF48576. SSF48576. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q38802-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLQYHVLNS IPSTTFLSST KTTISSSFLT ISGSPLNVAR DKSRSGSIHC
60 70 80 90 100
SKLRTQEYIN SQEVQHDLPL IHEWQQLQGE DAPQISVGSN SNAFKEAVKS
110 120 130 140 150
VKTILRNLTD GEITISAYDT AWVALIDAGD KTPAFPSAVK WIAENQLSDG
160 170 180 190 200
SWGDAYLFSY HDRLINTLAC VVALRSWNLF PHQCNKGITF FRENIGKLED
210 220 230 240 250
ENDEHMPIGF EVAFPSLLEI ARGINIDVPY DSPVLKDIYA KKELKLTRIP
260 270 280 290 300
KEIMHKIPTT LLHSLEGMRD LDWEKLLKLQ SQDGSFLFSP SSTAFAFMQT
310 320 330 340 350
RDSNCLEYLR NAVKRFNGGV PNVFPVDLFE HIWIVDRLQR LGISRYFEEE
360 370 380 390 400
IKECLDYVHR YWTDNGICWA RCSHVQDIDD TAMAFRLLRQ HGYQVSADVF
410 420 430 440 450
KNFEKEGEFF CFVGQSNQAV TGMFNLYRAS QLAFPREEIL KNAKEFSYNY
460 470 480 490 500
LLEKREREEL IDKWIIMKDL PGEIGFALEI PWYASLPRVE TRFYIDQYGG
510 520 530 540 550
ENDVWIGKTL YRMPYVNNNG YLELAKQDYN NCQAQHQLEW DIFQKWYEEN
560 570 580 590 600
RLSEWGVRRS ELLECYYLAA ATIFESERSH ERMVWAKSSV LVKAISSSFG
610 620 630 640 650
ESSDSRRSFS DQFHEYIANA RRSDHHFNDR NMRLDRPGSV QASRLAGVLI
660 670 680 690 700
GTLNQMSFDL FMSHGRDVNN LLYLSWGDWM EKWKLYGDEG EGELMVKMII
710 720 730 740 750
LMKNNDLTNF FTHTHFVRLA EIINRICLPR QYLKARRNDE KEKTIKSMEK
760 770 780 790 800
EMGKMVELAL SESDTFRDVS ITFLDVAKAF YYFALCGDHL QTHISKVLFQ

KV
Length:802
Mass (Da):93,014
Last modified:November 1, 1996 - v1
Checksum:iB2815AB0C06414F6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U11034 mRNA. Translation: AAA53632.1.
AC004044 Genomic DNA. Translation: AAD15334.1.
AL161495 Genomic DNA. Translation: CAB77763.1.
CP002687 Genomic DNA. Translation: AEE82229.1.
PIRiD85035.
RefSeqiNP_192187.1. NM_116512.1.
UniGeneiAt.355.

Genome annotation databases

EnsemblPlantsiAT4G02780.1; AT4G02780.1; AT4G02780.
GeneIDi828182.
GrameneiAT4G02780.1; AT4G02780.1; AT4G02780.
KEGGiath:AT4G02780.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U11034 mRNA. Translation: AAA53632.1.
AC004044 Genomic DNA. Translation: AAD15334.1.
AL161495 Genomic DNA. Translation: CAB77763.1.
CP002687 Genomic DNA. Translation: AEE82229.1.
PIRiD85035.
RefSeqiNP_192187.1. NM_116512.1.
UniGeneiAt.355.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3PYAX-ray2.25A85-802[»]
3PYBX-ray2.76A/B/C85-802[»]
4LIXX-ray1.55A85-802[»]
ProteinModelPortaliQ38802.
SMRiQ38802. Positions 91-802.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT4G02780.1.

Proteomic databases

PaxDbiQ38802.
PRIDEiQ38802.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G02780.1; AT4G02780.1; AT4G02780.
GeneIDi828182.
GrameneiAT4G02780.1; AT4G02780.1; AT4G02780.
KEGGiath:AT4G02780.

Organism-specific databases

TAIRiAT4G02780.

Phylogenomic databases

eggNOGiENOG410IHWJ. Eukaryota.
ENOG410XNXR. LUCA.
HOGENOMiHOG000241020.
InParanoidiQ38802.
KOiK04120.
OMAiKWIIMKD.
OrthoDBiEOG093603SF.
PhylomeDBiQ38802.

Enzyme and pathway databases

UniPathwayiUPA00390.
BioCyciARA:AT4G02780-MONOMER.
MetaCyc:AT4G02780-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ38802.
PROiQ38802.

Gene expression databases

GenevisibleiQ38802. AT.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
1.50.10.20. 1 hit.
1.50.30.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR001906. Terpene_synth_N.
IPR005630. Terpene_synthase_metal-bd.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF01397. Terpene_synth. 1 hit.
PF03936. Terpene_synth_C. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 3 hits.
SSF48576. SSF48576. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKSA_ARATH
AccessioniPrimary (citable) accession number: Q38802
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.