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Protein

Ent-copalyl diphosphate synthase, chloroplastic

Gene

GA1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of geranylgeranyl diphosphate to the gibberellin precursor ent-copalyl diphosphate.2 Publications

Catalytic activityi

Geranylgeranyl diphosphate = ent-copalyl diphosphate.

Cofactori

Mg2+1 Publication

Enzyme regulationi

Inhibited by high concentrations of magnesium.1 Publication

Pathwayi: gibberellin biosynthesis

This protein is involved in the pathway gibberellin biosynthesis, which is part of Plant hormone biosynthesis.
View all proteins of this organism that are known to be involved in the pathway gibberellin biosynthesis and in Plant hormone biosynthesis.

GO - Molecular functioni

  • ent-copalyl diphosphate synthase activity Source: TAIR
  • magnesium ion binding Source: TAIR
  • terpene synthase activity Source: InterPro

GO - Biological processi

  • gibberellic acid mediated signaling pathway Source: TAIR
  • gibberellin biosynthetic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciARA:AT4G02780-MONOMER.
MetaCyc:AT4G02780-MONOMER.
UniPathwayiUPA00390.

Names & Taxonomyi

Protein namesi
Recommended name:
Ent-copalyl diphosphate synthase, chloroplastic (EC:5.5.1.13)
Short name:
AtCPS
Short name:
Ent-CDP synthase
Alternative name(s):
Ent-kaurene synthase A
Short name:
KSA
Protein GA REQUIRING 1
Gene namesi
Name:GA1
Synonyms:ABC33, CPS, CPS1, TPSGA1
Ordered Locus Names:At4g02780
ORF Names:T5J8.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G02780.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi331H → A: Reduced activity, but no effect on substrate binding. Increased inhibition by Mg(2+). 1 Publication1
Mutagenesisi331H → R: Reduced catalytic activity, but loss of inhibition by Mg(2+). 1 Publication1
Mutagenesisi377D → A: Reduced catalytic activity, but no effect on substrate binding. Reduced inhibition by Mg(2+). 1 Publication1
Mutagenesisi379D → A: Loss of function. 1 Publication1
Mutagenesisi380D → A: Strongly reduced activity. Reduced inhibition by Mg(2+). 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 60ChloroplastSequence analysisAdd BLAST60
ChainiPRO_000003362361 – 802Ent-copalyl diphosphate synthase, chloroplasticAdd BLAST742

Post-translational modificationi

The N-terminus is blocked.

Proteomic databases

PaxDbiQ38802.

Expressioni

Tissue specificityi

Expressed in roots, leaves, flowers and also in siliques.1 Publication

Gene expression databases

GenevisibleiQ38802. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G02780.1.

Structurei

Secondary structure

1802
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi92 – 106Combined sources15
Helixi117 – 124Combined sources8
Beta strandi129 – 134Combined sources6
Helixi136 – 145Combined sources10
Beta strandi155 – 157Combined sources3
Helixi160 – 176Combined sources17
Helixi181 – 194Combined sources14
Helixi195 – 197Combined sources3
Helixi203 – 205Combined sources3
Helixi210 – 223Combined sources14
Turni232 – 234Combined sources3
Helixi235 – 248Combined sources14
Helixi251 – 254Combined sources4
Helixi260 – 267Combined sources8
Helixi273 – 276Combined sources4
Helixi277 – 279Combined sources3
Beta strandi284 – 286Combined sources3
Helixi290 – 300Combined sources11
Helixi303 – 315Combined sources13
Turni316 – 318Combined sources3
Beta strandi322 – 324Combined sources3
Helixi327 – 340Combined sources14
Helixi344 – 347Combined sources4
Helixi348 – 361Combined sources14
Beta strandi369 – 372Combined sources4
Helixi378 – 390Combined sources13
Helixi397 – 403Combined sources7
Helixi420 – 430Combined sources11
Helixi438 – 456Combined sources19
Beta strandi463 – 466Combined sources4
Helixi470 – 479Combined sources10
Helixi482 – 484Combined sources3
Helixi487 – 497Combined sources11
Helixi500 – 502Combined sources3
Beta strandi504 – 511Combined sources8
Turni514 – 516Combined sources3
Helixi519 – 549Combined sources31
Helixi552 – 555Combined sources4
Helixi559 – 570Combined sources12
Helixi576 – 578Combined sources3
Helixi579 – 599Combined sources21
Helixi603 – 615Combined sources13
Helixi640 – 664Combined sources25
Helixi669 – 685Combined sources17
Beta strandi686 – 688Combined sources3
Helixi691 – 701Combined sources11
Beta strandi703 – 705Combined sources3
Helixi708 – 712Combined sources5
Helixi714 – 726Combined sources13
Helixi742 – 760Combined sources19
Turni764 – 766Combined sources3
Helixi767 – 786Combined sources20
Helixi791 – 799Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3PYAX-ray2.25A85-802[»]
3PYBX-ray2.76A/B/C85-802[»]
4LIXX-ray1.55A85-802[»]
ProteinModelPortaliQ38802.
SMRiQ38802.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ38802.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi377 – 380DXDD motif4

Domaini

The Asp-Xaa-Asp-Asp (DXDD) motif is important for the catalytic activity through binding to Mg2+.

Sequence similaritiesi

Belongs to the terpene synthase family. Tpsc subfamily.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IHWJ. Eukaryota.
ENOG410XNXR. LUCA.
HOGENOMiHOG000241020.
InParanoidiQ38802.
KOiK04120.
OMAiKWIIMKD.
OrthoDBiEOG093603SF.
PhylomeDBiQ38802.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
1.50.10.20. 1 hit.
1.50.30.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR001906. Terpene_synth_N.
IPR005630. Terpene_synthase_metal-bd.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF01397. Terpene_synth. 1 hit.
PF03936. Terpene_synth_C. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 3 hits.
SSF48576. SSF48576. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q38802-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLQYHVLNS IPSTTFLSST KTTISSSFLT ISGSPLNVAR DKSRSGSIHC
60 70 80 90 100
SKLRTQEYIN SQEVQHDLPL IHEWQQLQGE DAPQISVGSN SNAFKEAVKS
110 120 130 140 150
VKTILRNLTD GEITISAYDT AWVALIDAGD KTPAFPSAVK WIAENQLSDG
160 170 180 190 200
SWGDAYLFSY HDRLINTLAC VVALRSWNLF PHQCNKGITF FRENIGKLED
210 220 230 240 250
ENDEHMPIGF EVAFPSLLEI ARGINIDVPY DSPVLKDIYA KKELKLTRIP
260 270 280 290 300
KEIMHKIPTT LLHSLEGMRD LDWEKLLKLQ SQDGSFLFSP SSTAFAFMQT
310 320 330 340 350
RDSNCLEYLR NAVKRFNGGV PNVFPVDLFE HIWIVDRLQR LGISRYFEEE
360 370 380 390 400
IKECLDYVHR YWTDNGICWA RCSHVQDIDD TAMAFRLLRQ HGYQVSADVF
410 420 430 440 450
KNFEKEGEFF CFVGQSNQAV TGMFNLYRAS QLAFPREEIL KNAKEFSYNY
460 470 480 490 500
LLEKREREEL IDKWIIMKDL PGEIGFALEI PWYASLPRVE TRFYIDQYGG
510 520 530 540 550
ENDVWIGKTL YRMPYVNNNG YLELAKQDYN NCQAQHQLEW DIFQKWYEEN
560 570 580 590 600
RLSEWGVRRS ELLECYYLAA ATIFESERSH ERMVWAKSSV LVKAISSSFG
610 620 630 640 650
ESSDSRRSFS DQFHEYIANA RRSDHHFNDR NMRLDRPGSV QASRLAGVLI
660 670 680 690 700
GTLNQMSFDL FMSHGRDVNN LLYLSWGDWM EKWKLYGDEG EGELMVKMII
710 720 730 740 750
LMKNNDLTNF FTHTHFVRLA EIINRICLPR QYLKARRNDE KEKTIKSMEK
760 770 780 790 800
EMGKMVELAL SESDTFRDVS ITFLDVAKAF YYFALCGDHL QTHISKVLFQ

KV
Length:802
Mass (Da):93,014
Last modified:November 1, 1996 - v1
Checksum:iB2815AB0C06414F6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U11034 mRNA. Translation: AAA53632.1.
AC004044 Genomic DNA. Translation: AAD15334.1.
AL161495 Genomic DNA. Translation: CAB77763.1.
CP002687 Genomic DNA. Translation: AEE82229.1.
PIRiD85035.
RefSeqiNP_192187.1. NM_116512.2.
UniGeneiAt.355.

Genome annotation databases

EnsemblPlantsiAT4G02780.1; AT4G02780.1; AT4G02780.
GeneIDi828182.
GrameneiAT4G02780.1; AT4G02780.1; AT4G02780.
KEGGiath:AT4G02780.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U11034 mRNA. Translation: AAA53632.1.
AC004044 Genomic DNA. Translation: AAD15334.1.
AL161495 Genomic DNA. Translation: CAB77763.1.
CP002687 Genomic DNA. Translation: AEE82229.1.
PIRiD85035.
RefSeqiNP_192187.1. NM_116512.2.
UniGeneiAt.355.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3PYAX-ray2.25A85-802[»]
3PYBX-ray2.76A/B/C85-802[»]
4LIXX-ray1.55A85-802[»]
ProteinModelPortaliQ38802.
SMRiQ38802.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT4G02780.1.

Proteomic databases

PaxDbiQ38802.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G02780.1; AT4G02780.1; AT4G02780.
GeneIDi828182.
GrameneiAT4G02780.1; AT4G02780.1; AT4G02780.
KEGGiath:AT4G02780.

Organism-specific databases

TAIRiAT4G02780.

Phylogenomic databases

eggNOGiENOG410IHWJ. Eukaryota.
ENOG410XNXR. LUCA.
HOGENOMiHOG000241020.
InParanoidiQ38802.
KOiK04120.
OMAiKWIIMKD.
OrthoDBiEOG093603SF.
PhylomeDBiQ38802.

Enzyme and pathway databases

UniPathwayiUPA00390.
BioCyciARA:AT4G02780-MONOMER.
MetaCyc:AT4G02780-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ38802.
PROiQ38802.

Gene expression databases

GenevisibleiQ38802. AT.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
1.50.10.20. 1 hit.
1.50.30.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR001906. Terpene_synth_N.
IPR005630. Terpene_synthase_metal-bd.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF01397. Terpene_synth. 1 hit.
PF03936. Terpene_synth_C. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 3 hits.
SSF48576. SSF48576. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKSA_ARATH
AccessioniPrimary (citable) accession number: Q38802
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.