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Protein

Pyruvate dehydrogenase E1 component subunit beta-1, mitochondrial

Gene

PDH2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).

Catalytic activityi

Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei92Thiamine pyrophosphateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processGlycolysis
LigandPyruvate, Thiamine pyrophosphate

Enzyme and pathway databases

BioCyciARA:AT5G50850-MONOMER.
ReactomeiR-ATH-204174. Regulation of pyruvate dehydrogenase (PDH) complex.
R-ATH-5362517. Signaling by Retinoic Acid.
R-ATH-70268. Pyruvate metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate dehydrogenase E1 component subunit beta-1, mitochondrial (EC:1.2.4.1)
Short name:
PDHE1-B
Alternative name(s):
Protein MACCI-BOU
Gene namesi
Name:PDH2
Synonyms:MAB1
Ordered Locus Names:At5g50850
ORF Names:K16E14.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G50850.
TAIRilocus:2152745. AT5G50850.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 29Mitochondrion1 PublicationAdd BLAST29
ChainiPRO_000002046230 – 363Pyruvate dehydrogenase E1 component subunit beta-1, mitochondrialAdd BLAST334

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki247Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki254Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ38799.
PRIDEiQ38799.

2D gel databases

SWISS-2DPAGEiQ38799.

PTM databases

iPTMnetiQ38799.

Expressioni

Tissue specificityi

Expressed in roots, immature rosettes, and mature rosettes.1 Publication

Gene expression databases

ExpressionAtlasiQ38799. baseline and differential.
GenevisibleiQ38799. AT.

Interactioni

Subunit structurei

Tetramer of 2 alpha and 2 beta subunits.By similarity

Protein-protein interaction databases

BioGridi20403. 2 interactors.
IntActiQ38799. 2 interactors.
STRINGi3702.AT5G50850.1.

Structurei

3D structure databases

ProteinModelPortaliQ38799.
SMRiQ38799.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0524. Eukaryota.
COG0022. LUCA.
HOGENOMiHOG000281450.
InParanoidiQ38799.
KOiK00162.
OMAiQEMTRDP.
OrthoDBiEOG09360DY5.
PhylomeDBiQ38799.

Family and domain databases

Gene3Di3.40.50.920. 1 hit.
InterProiView protein in InterPro
IPR027110. PDHB.
IPR029061. THDP-binding.
IPR009014. Transketo_C/PFOR_II.
IPR005475. Transketolase-like_Pyr-bd.
IPR033248. Transketolase_C.
PANTHERiPTHR11624:SF94. PTHR11624:SF94. 1 hit.
PfamiView protein in Pfam
PF02779. Transket_pyr. 1 hit.
PF02780. Transketolase_C. 1 hit.
SMARTiView protein in SMART
SM00861. Transket_pyr. 1 hit.
SUPFAMiSSF52518. SSF52518. 1 hit.
SSF52922. SSF52922. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q38799-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLGILRQRAI DGASTLRRTR FALVSARSYA AGAKEMTVRD ALNSAIDEEM
60 70 80 90 100
SADPKVFVMG EEVGQYQGAY KITKGLLEKY GPERVYDTPI TEAGFTGIGV
110 120 130 140 150
GAAYAGLKPV VEFMTFNFSM QAIDHIINSA AKSNYMSAGQ INVPIVFRGP
160 170 180 190 200
NGAAAGVGAQ HSQCYAAWYA SVPGLKVLAP YSAEDARGLL KAAIRDPDPV
210 220 230 240 250
VFLENELLYG ESFPISEEAL DSSFCLPIGK AKIEREGKDV TIVTFSKMVG
260 270 280 290 300
FALKAAEKLA EEGISAEVIN LRSIRPLDRA TINASVRKTS RLVTVEEGFP
310 320 330 340 350
QHGVCAEICA SVVEESFSYL DAPVERIAGA DVPMPYAANL ERLALPQIED
360
IVRASKRACY RSK
Length:363
Mass (Da):39,176
Last modified:June 6, 2002 - v2
Checksum:iD044E3A336D4EC52
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti334M → I in AAA52225 (PubMed:8061040).Curated1
Sequence conflicti337A → T in AAA52225 (PubMed:8061040).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09137 mRNA. Translation: AAA52225.1.
AB026637 Genomic DNA. Translation: BAA98121.1.
CP002688 Genomic DNA. Translation: AED96001.1.
AY070728 mRNA. Translation: AAL50070.1.
BT000839 mRNA. Translation: AAN38676.1.
RefSeqiNP_199898.1. NM_124463.4.
UniGeneiAt.24270.

Genome annotation databases

EnsemblPlantsiAT5G50850.1; AT5G50850.1; AT5G50850.
GeneIDi835157.
GrameneiAT5G50850.1; AT5G50850.1; AT5G50850.
KEGGiath:AT5G50850.

Similar proteinsi

Entry informationi

Entry nameiODPB1_ARATH
AccessioniPrimary (citable) accession number: Q38799
Secondary accession number(s): Q9LSM8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: June 6, 2002
Last modified: November 22, 2017
This is version 153 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names