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Protein

Pyruvate dehydrogenase E1 component subunit beta-1, mitochondrial

Gene

PDH2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).

Catalytic activityi

Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei92Thiamine pyrophosphateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Pyruvate, Thiamine pyrophosphate

Enzyme and pathway databases

BioCyciARA:AT5G50850-MONOMER.
ReactomeiR-ATH-70268. Pyruvate metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate dehydrogenase E1 component subunit beta-1, mitochondrial (EC:1.2.4.1)
Short name:
PDHE1-B
Alternative name(s):
Protein MACCI-BOU
Gene namesi
Name:PDH2
Synonyms:MAB1
Ordered Locus Names:At5g50850
ORF Names:K16E14.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G50850.

Subcellular locationi

GO - Cellular componenti

  • apoplast Source: TAIR
  • mitochondrial matrix Source: UniProtKB-SubCell
  • mitochondrion Source: TAIR
  • nucleolus Source: TAIR
  • vacuolar membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 29MitochondrionBy similarityAdd BLAST29
ChainiPRO_000002046230 – 363Pyruvate dehydrogenase E1 component subunit beta-1, mitochondrialAdd BLAST334

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki247Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki254Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ38799.
PRIDEiQ38799.

2D gel databases

SWISS-2DPAGEQ38799.

PTM databases

iPTMnetiQ38799.

Expressioni

Tissue specificityi

Expressed in roots, immature rosettes, and mature rosettes.1 Publication

Gene expression databases

GenevisibleiQ38799. AT.

Interactioni

Subunit structurei

Tetramer of 2 alpha and 2 beta subunits.By similarity

Protein-protein interaction databases

BioGridi20403. 2 interactors.
IntActiQ38799. 2 interactors.
STRINGi3702.AT5G50850.1.

Structurei

3D structure databases

ProteinModelPortaliQ38799.
SMRiQ38799.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0524. Eukaryota.
COG0022. LUCA.
HOGENOMiHOG000281450.
InParanoidiQ38799.
KOiK00162.
OMAiRKTHHLV.
OrthoDBiEOG09360DY5.
PhylomeDBiQ38799.

Family and domain databases

Gene3Di3.40.50.920. 1 hit.
3.40.50.970. 1 hit.
InterProiIPR027110. PDHB.
IPR029061. THDP-binding.
IPR009014. Transketo_C/PFOR_II.
IPR005475. Transketolase-like_Pyr-bd.
IPR033248. Transketolase_C.
IPR033247. Transketolase_fam.
[Graphical view]
PANTHERiPTHR11624. PTHR11624. 1 hit.
PTHR11624:SF56. PTHR11624:SF56. 1 hit.
PfamiPF02779. Transket_pyr. 1 hit.
PF02780. Transketolase_C. 1 hit.
[Graphical view]
SMARTiSM00861. Transket_pyr. 1 hit.
[Graphical view]
SUPFAMiSSF52518. SSF52518. 1 hit.
SSF52922. SSF52922. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q38799-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLGILRQRAI DGASTLRRTR FALVSARSYA AGAKEMTVRD ALNSAIDEEM
60 70 80 90 100
SADPKVFVMG EEVGQYQGAY KITKGLLEKY GPERVYDTPI TEAGFTGIGV
110 120 130 140 150
GAAYAGLKPV VEFMTFNFSM QAIDHIINSA AKSNYMSAGQ INVPIVFRGP
160 170 180 190 200
NGAAAGVGAQ HSQCYAAWYA SVPGLKVLAP YSAEDARGLL KAAIRDPDPV
210 220 230 240 250
VFLENELLYG ESFPISEEAL DSSFCLPIGK AKIEREGKDV TIVTFSKMVG
260 270 280 290 300
FALKAAEKLA EEGISAEVIN LRSIRPLDRA TINASVRKTS RLVTVEEGFP
310 320 330 340 350
QHGVCAEICA SVVEESFSYL DAPVERIAGA DVPMPYAANL ERLALPQIED
360
IVRASKRACY RSK
Length:363
Mass (Da):39,176
Last modified:June 6, 2002 - v2
Checksum:iD044E3A336D4EC52
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti334M → I in AAA52225 (PubMed:8061040).Curated1
Sequence conflicti337A → T in AAA52225 (PubMed:8061040).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09137 mRNA. Translation: AAA52225.1.
AB026637 Genomic DNA. Translation: BAA98121.1.
CP002688 Genomic DNA. Translation: AED96001.1.
AY070728 mRNA. Translation: AAL50070.1.
BT000839 mRNA. Translation: AAN38676.1.
RefSeqiNP_199898.1. NM_124463.4.
UniGeneiAt.24270.

Genome annotation databases

EnsemblPlantsiAT5G50850.1; AT5G50850.1; AT5G50850.
GeneIDi835157.
GrameneiAT5G50850.1; AT5G50850.1; AT5G50850.
KEGGiath:AT5G50850.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09137 mRNA. Translation: AAA52225.1.
AB026637 Genomic DNA. Translation: BAA98121.1.
CP002688 Genomic DNA. Translation: AED96001.1.
AY070728 mRNA. Translation: AAL50070.1.
BT000839 mRNA. Translation: AAN38676.1.
RefSeqiNP_199898.1. NM_124463.4.
UniGeneiAt.24270.

3D structure databases

ProteinModelPortaliQ38799.
SMRiQ38799.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi20403. 2 interactors.
IntActiQ38799. 2 interactors.
STRINGi3702.AT5G50850.1.

PTM databases

iPTMnetiQ38799.

2D gel databases

SWISS-2DPAGEQ38799.

Proteomic databases

PaxDbiQ38799.
PRIDEiQ38799.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G50850.1; AT5G50850.1; AT5G50850.
GeneIDi835157.
GrameneiAT5G50850.1; AT5G50850.1; AT5G50850.
KEGGiath:AT5G50850.

Organism-specific databases

TAIRiAT5G50850.

Phylogenomic databases

eggNOGiKOG0524. Eukaryota.
COG0022. LUCA.
HOGENOMiHOG000281450.
InParanoidiQ38799.
KOiK00162.
OMAiRKTHHLV.
OrthoDBiEOG09360DY5.
PhylomeDBiQ38799.

Enzyme and pathway databases

BioCyciARA:AT5G50850-MONOMER.
ReactomeiR-ATH-70268. Pyruvate metabolism.

Miscellaneous databases

PROiQ38799.

Gene expression databases

GenevisibleiQ38799. AT.

Family and domain databases

Gene3Di3.40.50.920. 1 hit.
3.40.50.970. 1 hit.
InterProiIPR027110. PDHB.
IPR029061. THDP-binding.
IPR009014. Transketo_C/PFOR_II.
IPR005475. Transketolase-like_Pyr-bd.
IPR033248. Transketolase_C.
IPR033247. Transketolase_fam.
[Graphical view]
PANTHERiPTHR11624. PTHR11624. 1 hit.
PTHR11624:SF56. PTHR11624:SF56. 1 hit.
PfamiPF02779. Transket_pyr. 1 hit.
PF02780. Transketolase_C. 1 hit.
[Graphical view]
SMARTiSM00861. Transket_pyr. 1 hit.
[Graphical view]
SUPFAMiSSF52518. SSF52518. 1 hit.
SSF52922. SSF52922. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiODPB1_ARATH
AccessioniPrimary (citable) accession number: Q38799
Secondary accession number(s): Q9LSM8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: June 6, 2002
Last modified: November 30, 2016
This is version 146 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.