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Protein

Calnexin homolog 2

Gene

At5g07340

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi34 – 341Calcium; via carbonyl oxygenBy similarity
Metal bindingi65 – 651Calcium; via carbonyl oxygenBy similarity
Binding sitei114 – 1141CarbohydrateBy similarity
Binding sitei116 – 1161CarbohydrateBy similarity
Binding sitei136 – 1361CarbohydrateBy similarity
Binding sitei167 – 1671CarbohydrateBy similarity
Metal bindingi384 – 3841CalciumBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Ligandi

Calcium, Lectin, Metal-binding

Enzyme and pathway databases

ReactomeiR-ATH-901042. Calnexin/calreticulin cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Calnexin homolog 2
Gene namesi
Ordered Locus Names:At5g07340
ORF Names:T2I1_50
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G07340.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini26 – 468443LumenalSequence analysisAdd
BLAST
Transmembranei469 – 48921HelicalSequence analysisAdd
BLAST
Topological domaini490 – 53243CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence analysisAdd
BLAST
Chaini26 – 532507Calnexin homolog 2PRO_0000004203Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi110 ↔ 145By similarity
Disulfide bondi309 ↔ 315By similarity
Glycosylationi466 – 4661N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ38798.
PRIDEiQ38798.

PTM databases

SwissPalmiQ38798.

Expressioni

Gene expression databases

ExpressionAtlasiQ38798. baseline and differential.
GenevisibleiQ38798. AT.

Interactioni

Protein-protein interaction databases

BioGridi15904. 24 interactions.
STRINGi3702.AT5G07340.2.

Structurei

3D structure databases

ProteinModelPortaliQ38798.
SMRiQ38798. Positions 40-403.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati227 – 238121-1Add
BLAST
Repeati244 – 255121-2Add
BLAST
Repeati263 – 274121-3Add
BLAST
Repeati282 – 293121-4Add
BLAST
Repeati297 – 307112-1Add
BLAST
Repeati316 – 326112-2Add
BLAST
Repeati330 – 340112-3Add
BLAST
Repeati344 – 354112-4Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni225 – 358134P domain (Extended arm)By similarityAdd
BLAST
Regioni227 – 293674 X approximate repeatsAdd
BLAST
Regioni297 – 354584 X approximate repeatsAdd
BLAST

Sequence similaritiesi

Belongs to the calreticulin family.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0675. Eukaryota.
ENOG410XP7T. LUCA.
HOGENOMiHOG000192435.
InParanoidiQ38798.
KOiK08054.
PhylomeDBiQ38798.

Family and domain databases

Gene3Di2.10.250.10. 1 hit.
2.60.120.200. 1 hit.
InterProiIPR001580. Calret/calnex.
IPR018124. Calret/calnex_CS.
IPR009033. Calreticulin/calnexin_P_dom.
IPR013320. ConA-like_dom.
[Graphical view]
PANTHERiPTHR11073. PTHR11073. 1 hit.
PfamiPF00262. Calreticulin. 1 hit.
[Graphical view]
PRINTSiPR00626. CALRETICULIN.
SUPFAMiSSF49899. SSF49899. 2 hits.
SSF63887. SSF63887. 1 hit.
PROSITEiPS00803. CALRETICULIN_1. 1 hit.
PS00804. CALRETICULIN_2. 1 hit.
PS00805. CALRETICULIN_REPEAT. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q38798-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRERIITFVS LLLVALLSFP SVSYCDDQTI LYESFDEPFD GRWVVSEKAE
60 70 80 90 100
YQGVWKHEKS EGHDDYGLLV SEKAKKYGIV KELDVDEPLN LNEGTVVLQY
110 120 130 140 150
EARFQEGLEC GGAYLKYLRP QEAGWVPQGF DNDSPYSIMF GPDKCGATNK
160 170 180 190 200
VHFILKHKNP KSGEFVEHHL KFPPSVPFDM LSHVYTAVLK SDNEVRILVD
210 220 230 240 250
GEEKKKGNLL SAEDFEPPLI PSKTIPDPED KKPEDWDERA KIPDPNAVKP
260 270 280 290 300
DDWDEDAPME IEDEEAEKPE GWLDDEPVEV EDPEASKPED WDDEEDGEWE
310 320 330 340 350
APKVSNTKCE AAPGCGEWKR PMKRNPAYKG KWSSPLIDNP AYKGIWKPRD
360 370 380 390 400
IPNPDYFELE RPNLEPIAAI GIEIWTMQDG ILFDNILISK DEKVAETYRQ
410 420 430 440 450
STWKPKFDVE KEKQKAEDEA AGEADGLKSY QKKVFDLLYK VADISFLSAY
460 470 480 490 500
KSKIMELIEK AETQPNLTIG VLISIVIVFL SLFFKLIFGG AKAKVEKKKP
510 520 530
ETAAETSTSE AKTEEKAEAV AAPRKRQTRR ES
Length:532
Mass (Da):60,490
Last modified:December 13, 2002 - v2
Checksum:i2283669E7A5C6D1C
GO

Sequence cautioni

The sequence AAA17742 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti30 – 301I → V in AAA17742 (PubMed:7846171).Curated
Sequence conflicti180 – 1801M → K in AAA17742 (PubMed:7846171).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U08315 Genomic DNA. Translation: AAA17742.1. Sequence problems.
AL163912 Genomic DNA. Translation: CAB87923.1.
CP002688 Genomic DNA. Translation: AED91138.1.
BT001999 mRNA. Translation: AAN72010.1.
BT010385 mRNA. Translation: AAQ56828.1.
PIRiT49873.
RefSeqiNP_196351.1. NM_120816.2. [Q38798-1]
UniGeneiAt.43202.

Genome annotation databases

EnsemblPlantsiAT5G07340.1; AT5G07340.1; AT5G07340. [Q38798-1]
GeneIDi830625.
KEGGiath:AT5G07340.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U08315 Genomic DNA. Translation: AAA17742.1. Sequence problems.
AL163912 Genomic DNA. Translation: CAB87923.1.
CP002688 Genomic DNA. Translation: AED91138.1.
BT001999 mRNA. Translation: AAN72010.1.
BT010385 mRNA. Translation: AAQ56828.1.
PIRiT49873.
RefSeqiNP_196351.1. NM_120816.2. [Q38798-1]
UniGeneiAt.43202.

3D structure databases

ProteinModelPortaliQ38798.
SMRiQ38798. Positions 40-403.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi15904. 24 interactions.
STRINGi3702.AT5G07340.2.

PTM databases

SwissPalmiQ38798.

Proteomic databases

PaxDbiQ38798.
PRIDEiQ38798.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G07340.1; AT5G07340.1; AT5G07340. [Q38798-1]
GeneIDi830625.
KEGGiath:AT5G07340.

Organism-specific databases

TAIRiAT5G07340.

Phylogenomic databases

eggNOGiKOG0675. Eukaryota.
ENOG410XP7T. LUCA.
HOGENOMiHOG000192435.
InParanoidiQ38798.
KOiK08054.
PhylomeDBiQ38798.

Enzyme and pathway databases

ReactomeiR-ATH-901042. Calnexin/calreticulin cycle.

Miscellaneous databases

PROiQ38798.

Gene expression databases

ExpressionAtlasiQ38798. baseline and differential.
GenevisibleiQ38798. AT.

Family and domain databases

Gene3Di2.10.250.10. 1 hit.
2.60.120.200. 1 hit.
InterProiIPR001580. Calret/calnex.
IPR018124. Calret/calnex_CS.
IPR009033. Calreticulin/calnexin_P_dom.
IPR013320. ConA-like_dom.
[Graphical view]
PANTHERiPTHR11073. PTHR11073. 1 hit.
PfamiPF00262. Calreticulin. 1 hit.
[Graphical view]
PRINTSiPR00626. CALRETICULIN.
SUPFAMiSSF49899. SSF49899. 2 hits.
SSF63887. SSF63887. 1 hit.
PROSITEiPS00803. CALRETICULIN_1. 1 hit.
PS00804. CALRETICULIN_2. 1 hit.
PS00805. CALRETICULIN_REPEAT. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCALX2_ARATH
AccessioniPrimary (citable) accession number: Q38798
Secondary accession number(s): Q9LY26
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 13, 2002
Last modified: June 8, 2016
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.