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Protein
Submitted name:

Lanosterol 14-alpha-demethylase

Gene

Tb11.02.4080

Organism
Trypanosoma brucei brucei (strain 927/4 GUTat10.1)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi422 – 4221Iron (heme axial ligand)Combined sources

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

MethyltransferaseImported, MonooxygenaseUniRule annotation, Oxidoreductase, Transferase

Keywords - Ligandi

HemeCombined sources, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Submitted name:
Lanosterol 14-alpha-demethylaseImported (EC:1.14.13.70Imported)
Gene namesi
ORF Names:Tb11.02.4080Imported
OrganismiTrypanosoma brucei brucei (strain 927/4 GUTat10.1)Imported
Taxonomic identifieri185431 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosoma
ProteomesiUP000008524 Componenti: Chromosome 11 Scaffold 1

Interactioni

Protein-protein interaction databases

STRINGi5691.EAN79583.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2WV2X-ray2.70A22-481[»]
2X2NX-ray2.60A/B/C/D22-481[»]
3G1QX-ray1.89A/B/C/D29-476[»]
3GW9X-ray1.87A/B/C/D29-477[»]
3P99X-ray3.00A/B/C/D29-481[»]
3TIKX-ray2.05A/B/C/D32-481[»]
4BJKX-ray2.67A/B/C/D32-481[»]
4G3JX-ray1.83A/B/C/D29-476[»]
4G7GX-ray2.05A/B/C/D29-476[»]
ProteinModelPortaliQ385E8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ385E8.

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000042780.
InParanoidiQ385E8.
KOiK05917.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00465. EP450IV.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q385E8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLEVAIFLL TALALYSFYF VKSFNVTRPT DPPVYPVTVP ILGHIIQFGK
60 70 80 90 100
SPLGFMQECK RQLKSGIFTI NIVGKRVTIV GDPHEHSRFF LPRNEVLSPR
110 120 130 140 150
EVYSFMVPVF GEGVAYAAPY PRMREQLNFL AEELTIAKFQ NFVPAIQHEV
160 170 180 190 200
RKFMAANWDK DEGEINLLED CSTMIINTAC QCLFGEDLRK RLDARRFAQL
210 220 230 240 250
LAKMESSLIP AAVFLPILLK LPLPQSARCH EARTELQKIL SEIIIARKEE
260 270 280 290 300
EVNKDSSTSD LLSGLLSAVY RDGTPMSLHE VCGMIVAAMF AGQHTSSITT
310 320 330 340 350
TWSMLHLMHP ANVKHLEALR KEIEEFPAQL NYNNVMDEMP FAERCARESI
360 370 380 390 400
RRDPPLLMLM RKVMADVKVG SYVVPKGDII ACSPLLSHHD EEAFPEPRRW
410 420 430 440 450
DPERDEKVEG AFIGFGAGVH KCIGQKFGLL QVKTILATAF RSYDFQLLRD
460 470 480
EVPDPDYHTM VVGPTASQCR VKYIRRKAAA A
Length:481
Mass (Da):54,372
Last modified:November 22, 2005 - v1
Checksum:i10579D3A25BDF6F4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH464491 Genomic DNA. Translation: EAN79583.1.
RefSeqiXP_828695.1. XM_823602.1.

Genome annotation databases

GeneIDi3665105.
KEGGitbr:Tb11.02.4080.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH464491 Genomic DNA. Translation: EAN79583.1.
RefSeqiXP_828695.1. XM_823602.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2WV2X-ray2.70A22-481[»]
2X2NX-ray2.60A/B/C/D22-481[»]
3G1QX-ray1.89A/B/C/D29-476[»]
3GW9X-ray1.87A/B/C/D29-477[»]
3P99X-ray3.00A/B/C/D29-481[»]
3TIKX-ray2.05A/B/C/D32-481[»]
4BJKX-ray2.67A/B/C/D32-481[»]
4G3JX-ray1.83A/B/C/D29-476[»]
4G7GX-ray2.05A/B/C/D29-476[»]
ProteinModelPortaliQ385E8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi5691.EAN79583.

Chemistry

BindingDBiQ385E8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi3665105.
KEGGitbr:Tb11.02.4080.

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000042780.
InParanoidiQ385E8.
KOiK05917.

Miscellaneous databases

EvolutionaryTraceiQ385E8.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00465. EP450IV.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome of the African trypanosome Trypanosoma brucei."
    Berriman M., Ghedin E., Hertz-Fowler C., Blandin G., Renauld H., Bartholomeu D.C., Lennard N.J., Caler E., Hamlin N.E., Haas B., Bohme U., Hannick L., Aslett M.A., Shallom J., Marcello L., Hou L., Wickstead B., Alsmark U.C.M.
    , Arrowsmith C., Atkin R.J., Barron A.J., Bringaud F., Brooks K., Carrington M., Cherevach I., Chillingworth T.J., Churcher C., Clark L.N., Corton C.H., Cronin A., Davies R.M., Doggett J., Djikeng A., Feldblyum T., Field M.C., Fraser A., Goodhead I., Hance Z., Harper D., Harris B.R., Hauser H., Hostetler J., Ivens A., Jagels K., Johnson D., Johnson J., Jones K., Kerhornou A.X., Koo H., Larke N., Landfear S., Larkin C., Leech V., Line A., Lord A., Macleod A., Mooney P.J., Moule S., Martin D.M., Morgan G.W., Mungall K., Norbertczak H., Ormond D., Pai G., Peacock C.S., Peterson J., Quail M.A., Rabbinowitsch E., Rajandream M.A., Reitter C., Salzberg S.L., Sanders M., Schobel S., Sharp S., Simmonds M., Simpson A.J., Tallon L., Turner C.M., Tait A., Tivey A.R., Van Aken S., Walker D., Wanless D., Wang S., White B., White O., Whitehead S., Woodward J., Wortman J., Adams M.D., Embley T.M., Gull K., Ullu E., Barry J.D., Fairlamb A.H., Opperdoes F., Barrell B.G., Donelson J.E., Hall N., Fraser C.M., Melville S.E., El-Sayed N.M.A.
    Science 309:416-422(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 927/4 GUTat10.1Imported.
  2. "Crystal structures of Trypanosoma brucei sterol 14alpha-demethylase and implications for selective treatment of human infections."
    Lepesheva G.I., Park H.W., Hargrove T.Y., Vanhollebeke B., Wawrzak Z., Harp J.M., Sundaramoorthy M., Nes W.D., Pays E., Chaudhuri M., Villalta F., Waterman M.R.
    J. Biol. Chem. 285:1773-1780(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.87 ANGSTROMS) OF 29-477 IN COMPLEX WITH HEME.
  3. "Structural characterization of CYP51 from Trypanosoma cruzi and Trypanosoma brucei bound to the antifungal drugs posaconazole and fluconazole."
    Chen C.K., Leung S.S., Guilbert C., Jacobson M.P., McKerrow J.H., Podust L.M.
    PLoS Negl. Trop. Dis. 4:e651-e651(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS) OF 22-481 IN COMPLEX WITH HEME.
  4. "Pharmacological characterization, structural studies, and in vivo activities of anti-Chagas disease lead compounds derived from tipifarnib."
    Buckner F.S., Bahia M.T., Suryadevara P.K., White K.L., Shackleford D.M., Chennamaneni N.K., Hulverson M.A., Laydbak J.U., Chatelain E., Scandale I., Verlinde C.L., Charman S.A., Lepesheva G.I., Gelb M.H.
    Antimicrob. Agents Chemother. 56:4914-4921(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS) OF 32-481 IN COMPLEX WITH HEME.
  5. "Structural complex of sterol 14alpha-demethylase (CYP51) with 14alpha-methylenecyclopropyl-Delta7-24, 25-dihydrolanosterol."
    Hargrove T.Y., Wawrzak Z., Liu J., Waterman M.R., Nes W.D., Lepesheva G.I.
    J. Lipid Res. 53:311-320(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.00 ANGSTROMS) OF 29-481 IN COMPLEX WITH HEME.
  6. "Rational development of 4-aminopyridyl-based inhibitors targeting Trypanosoma cruzi CYP51 as anti-chagas agents."
    Choi J.Y., Calvet C.M., Gunatilleke S.S., Ruiz C., Cameron M.D., McKerrow J.H., Podust L.M., Roush W.R.
    J. Med. Chem. 56:7651-7668(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.67 ANGSTROMS) OF 32-481 IN COMPLEX WITH HEME.
  7. "VFV as a new effective CYP51 structure-derived drug candidate for Chagas disease and visceral leishmaniasis."
    Lepesheva G.I., Hargrove T.Y., Rachakonda G., Wawrzak Z., Pomel S., Cojean S., Nde P.N., Nes W.D., Locuson C.W., Calcutt M.W., Waterman M.R., Daniels J.S., Loiseau P.M., Villalta F.
    J. Infect. Dis. 0:0-0(2015) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.83 ANGSTROMS) OF 29-476 IN COMPLEX WITH HEME.

Entry informationi

Entry nameiQ385E8_TRYB2
AccessioniPrimary (citable) accession number: Q385E8
Entry historyi
Integrated into UniProtKB/TrEMBL: November 22, 2005
Last sequence update: November 22, 2005
Last modified: June 24, 2015
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources, Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.