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Q385E8

- Q385E8_TRYB2

UniProt

Q385E8 - Q385E8_TRYB2

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Protein
Submitted name:

Lanosterol 14-alpha-demethylase

Gene

Tb11.02.4080

Organism
Trypanosoma brucei brucei (strain 927/4 GUTat10.1)
Status
Unreviewed - Annotation score: 1 out of 5- Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi422 – 4221Iron (heme axial ligand)Imported

GO - Molecular functioni

  1. heme binding Source: InterPro
  2. iron ion binding Source: InterPro
  3. methyltransferase activity Source: UniProtKB-KW
  4. sterol 14-demethylase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

MethyltransferaseImported, MonooxygenaseUniRule annotation, Oxidoreductase, Transferase

Keywords - Ligandi

HemeUniRule annotationImported, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Submitted name:
Lanosterol 14-alpha-demethylaseImported (EC:1.14.13.70Imported)
Gene namesi
ORF Names:Tb11.02.4080Imported
OrganismiTrypanosoma brucei brucei (strain 927/4 GUTat10.1)Imported
Taxonomic identifieri185431 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosoma
ProteomesiUP000008524: Chromosome 11 Scaffold 1

Interactioni

Protein-protein interaction databases

STRINGi5691.Tb11.02.4080.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2WV2X-ray2.70A22-481[»]
2X2NX-ray2.60A/B/C/D22-481[»]
3G1QX-ray1.89A/B/C/D29-476[»]
3GW9X-ray1.87A/B/C/D29-477[»]
3P99X-ray3.00A/B/C/D29-481[»]
3TIKX-ray2.05A/B/C/D32-481[»]
4BJKX-ray2.67A/B/C/D32-481[»]
4G3JX-ray1.83A/B/C/D29-476[»]
4G7GX-ray2.05A/B/C/D29-476[»]
ProteinModelPortaliQ385E8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ385E8.

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000042780.
InParanoidiQ385E8.
KOiK05917.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00465. EP450IV.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q385E8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLLEVAIFLL TALALYSFYF VKSFNVTRPT DPPVYPVTVP ILGHIIQFGK
60 70 80 90 100
SPLGFMQECK RQLKSGIFTI NIVGKRVTIV GDPHEHSRFF LPRNEVLSPR
110 120 130 140 150
EVYSFMVPVF GEGVAYAAPY PRMREQLNFL AEELTIAKFQ NFVPAIQHEV
160 170 180 190 200
RKFMAANWDK DEGEINLLED CSTMIINTAC QCLFGEDLRK RLDARRFAQL
210 220 230 240 250
LAKMESSLIP AAVFLPILLK LPLPQSARCH EARTELQKIL SEIIIARKEE
260 270 280 290 300
EVNKDSSTSD LLSGLLSAVY RDGTPMSLHE VCGMIVAAMF AGQHTSSITT
310 320 330 340 350
TWSMLHLMHP ANVKHLEALR KEIEEFPAQL NYNNVMDEMP FAERCARESI
360 370 380 390 400
RRDPPLLMLM RKVMADVKVG SYVVPKGDII ACSPLLSHHD EEAFPEPRRW
410 420 430 440 450
DPERDEKVEG AFIGFGAGVH KCIGQKFGLL QVKTILATAF RSYDFQLLRD
460 470 480
EVPDPDYHTM VVGPTASQCR VKYIRRKAAA A
Length:481
Mass (Da):54,372
Last modified:November 22, 2005 - v1
Checksum:i10579D3A25BDF6F4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CH464491 Genomic DNA. Translation: EAN79583.1.
RefSeqiXP_828695.1. XM_823602.1.

Genome annotation databases

GeneIDi3665105.
KEGGitbr:Tb11.02.4080.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CH464491 Genomic DNA. Translation: EAN79583.1 .
RefSeqi XP_828695.1. XM_823602.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2WV2 X-ray 2.70 A 22-481 [» ]
2X2N X-ray 2.60 A/B/C/D 22-481 [» ]
3G1Q X-ray 1.89 A/B/C/D 29-476 [» ]
3GW9 X-ray 1.87 A/B/C/D 29-477 [» ]
3P99 X-ray 3.00 A/B/C/D 29-481 [» ]
3TIK X-ray 2.05 A/B/C/D 32-481 [» ]
4BJK X-ray 2.67 A/B/C/D 32-481 [» ]
4G3J X-ray 1.83 A/B/C/D 29-476 [» ]
4G7G X-ray 2.05 A/B/C/D 29-476 [» ]
ProteinModelPortali Q385E8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5691.Tb11.02.4080.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 3665105.
KEGGi tbr:Tb11.02.4080.

Phylogenomic databases

eggNOGi COG2124.
HOGENOMi HOG000042780.
InParanoidi Q385E8.
KOi K05917.

Miscellaneous databases

EvolutionaryTracei Q385E8.

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00465. EP450IV.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome of the African trypanosome Trypanosoma brucei."
    Berriman M., Ghedin E., Hertz-Fowler C., Blandin G., Renauld H., Bartholomeu D.C., Lennard N.J., Caler E., Hamlin N.E., Haas B., Bohme U., Hannick L., Aslett M.A., Shallom J., Marcello L., Hou L., Wickstead B., Alsmark U.C.M.
    , Arrowsmith C., Atkin R.J., Barron A.J., Bringaud F., Brooks K., Carrington M., Cherevach I., Chillingworth T.J., Churcher C., Clark L.N., Corton C.H., Cronin A., Davies R.M., Doggett J., Djikeng A., Feldblyum T., Field M.C., Fraser A., Goodhead I., Hance Z., Harper D., Harris B.R., Hauser H., Hostetler J., Ivens A., Jagels K., Johnson D., Johnson J., Jones K., Kerhornou A.X., Koo H., Larke N., Landfear S., Larkin C., Leech V., Line A., Lord A., Macleod A., Mooney P.J., Moule S., Martin D.M., Morgan G.W., Mungall K., Norbertczak H., Ormond D., Pai G., Peacock C.S., Peterson J., Quail M.A., Rabbinowitsch E., Rajandream M.A., Reitter C., Salzberg S.L., Sanders M., Schobel S., Sharp S., Simmonds M., Simpson A.J., Tallon L., Turner C.M., Tait A., Tivey A.R., Van Aken S., Walker D., Wanless D., Wang S., White B., White O., Whitehead S., Woodward J., Wortman J., Adams M.D., Embley T.M., Gull K., Ullu E., Barry J.D., Fairlamb A.H., Opperdoes F., Barrell B.G., Donelson J.E., Hall N., Fraser C.M., Melville S.E., El-Sayed N.M.A.
    Science 309:416-422(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 927/4 GUTat10.1Imported.
  2. "Crystal structures of Trypanosoma brucei sterol 14alpha-demethylase and implications for selective treatment of human infections."
    Lepesheva G.I., Park H.W., Hargrove T.Y., Vanhollebeke B., Wawrzak Z., Harp J.M., Sundaramoorthy M., Nes W.D., Pays E., Chaudhuri M., Villalta F., Waterman M.R.
    J. Biol. Chem. 285:1773-1780(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.87 ANGSTROMS) OF 29-477 IN COMPLEX WITH HEME.
  3. "Structural characterization of CYP51 from Trypanosoma cruzi and Trypanosoma brucei bound to the antifungal drugs posaconazole and fluconazole."
    Chen C.K., Leung S.S., Guilbert C., Jacobson M.P., McKerrow J.H., Podust L.M.
    PLoS Negl. Trop. Dis. 4:e651-e651(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS) OF 22-481 IN COMPLEX WITH HEME.
  4. "Pharmacological characterization, structural studies, and in vivo activities of anti-Chagas disease lead compounds derived from tipifarnib."
    Buckner F.S., Bahia M.T., Suryadevara P.K., White K.L., Shackleford D.M., Chennamaneni N.K., Hulverson M.A., Laydbak J.U., Chatelain E., Scandale I., Verlinde C.L., Charman S.A., Lepesheva G.I., Gelb M.H.
    Antimicrob. Agents Chemother. 56:4914-4921(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS) OF 32-481 IN COMPLEX WITH HEME.
  5. "Structural complex of sterol 14?-demethylase (CYP51) with 14?-methylenecyclopropyl-Delta7-24, 25-dihydrolanosterol."
    Hargrove T.Y., Wawrzak Z., Liu J., Waterman M.R., Nes W.D., Lepesheva G.I.
    J. Lipid Res. 53:311-320(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.00 ANGSTROMS) OF 29-481 IN COMPLEX WITH HEME.
  6. "CYP51 structure-based VNI scaffold development."
    Hargrove T.Y., Wawrzak Z., Waterman M.R., Lepesheva G.I.
    Submitted (JUL-2012) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (1.83 ANGSTROMS) OF 29-476 IN COMPLEX WITH HEME.
  7. "Rational development of 4-aminopyridyl-based inhibitors targeting Trypanosoma cruzi CYP51 as anti-chagas agents."
    Choi J.Y., Calvet C.M., Gunatilleke S.S., Ruiz C., Cameron M.D., McKerrow J.H., Podust L.M., Roush W.R.
    J. Med. Chem. 56:7651-7668(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.67 ANGSTROMS) OF 32-481 IN COMPLEX WITH HEME.

Entry informationi

Entry nameiQ385E8_TRYB2
AccessioniPrimary (citable) accession number: Q385E8
Entry historyi
Integrated into UniProtKB/TrEMBL: November 22, 2005
Last sequence update: November 22, 2005
Last modified: October 29, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureImported, Complete proteome, Reference proteomeImported

External Data

Dasty 3