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Q385E8

- Q385E8_TRYB2

UniProt

Q385E8 - Q385E8_TRYB2

Protein
Submitted name:

Lanosterol 14-alpha-demethylase

Gene

Tb11.02.4080

Organism
Trypanosoma brucei brucei (strain 927/4 GUTat10.1)
Status
Unreviewed - Annotation score: 1 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (22 Nov 2005)
      Previous versions | rss
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    Functioni

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi422 – 4221Iron (heme axial ligand)Imported

    GO - Molecular functioni

    1. heme binding Source: InterPro
    2. iron ion binding Source: InterPro
    3. methyltransferase activity Source: UniProtKB-KW
    4. sterol 14-demethylase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    MethyltransferaseImported, MonooxygenaseUniRule annotation, Oxidoreductase, Transferase

    Keywords - Ligandi

    HemeUniRule annotationImported, Iron, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    Lanosterol 14-alpha-demethylaseImported (EC:1.14.13.70Imported)
    Gene namesi
    ORF Names:Tb11.02.4080Imported
    OrganismiTrypanosoma brucei brucei (strain 927/4 GUTat10.1)Imported
    Taxonomic identifieri999953 [NCBI]
    Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosoma
    ProteomesiUP000008524: Chromosome 11 Scaffold 1

    Interactioni

    Protein-protein interaction databases

    STRINGi5691.Tb11.02.4080.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2WV2X-ray2.70A22-481[»]
    2X2NX-ray2.60A/B/C/D22-481[»]
    3G1QX-ray1.89A/B/C/D29-476[»]
    3GW9X-ray1.87A/B/C/D29-477[»]
    3P99X-ray3.00A/B/C/D29-481[»]
    3TIKX-ray2.05A/B/C/D32-481[»]
    4BJKX-ray2.67A/B/C/D32-481[»]
    4G3JX-ray1.83A/B/C/D29-476[»]
    4G7GX-ray2.05A/B/C/D29-476[»]
    ProteinModelPortaliQ385E8.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ385E8.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2124.
    HOGENOMiHOG000042780.
    KOiK05917.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002403. Cyt_P450_E_grp-IV.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00465. EP450IV.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q385E8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLLEVAIFLL TALALYSFYF VKSFNVTRPT DPPVYPVTVP ILGHIIQFGK    50
    SPLGFMQECK RQLKSGIFTI NIVGKRVTIV GDPHEHSRFF LPRNEVLSPR 100
    EVYSFMVPVF GEGVAYAAPY PRMREQLNFL AEELTIAKFQ NFVPAIQHEV 150
    RKFMAANWDK DEGEINLLED CSTMIINTAC QCLFGEDLRK RLDARRFAQL 200
    LAKMESSLIP AAVFLPILLK LPLPQSARCH EARTELQKIL SEIIIARKEE 250
    EVNKDSSTSD LLSGLLSAVY RDGTPMSLHE VCGMIVAAMF AGQHTSSITT 300
    TWSMLHLMHP ANVKHLEALR KEIEEFPAQL NYNNVMDEMP FAERCARESI 350
    RRDPPLLMLM RKVMADVKVG SYVVPKGDII ACSPLLSHHD EEAFPEPRRW 400
    DPERDEKVEG AFIGFGAGVH KCIGQKFGLL QVKTILATAF RSYDFQLLRD 450
    EVPDPDYHTM VVGPTASQCR VKYIRRKAAA A 481
    Length:481
    Mass (Da):54,372
    Last modified:November 22, 2005 - v1
    Checksum:i10579D3A25BDF6F4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH464491 Genomic DNA. Translation: EAN79583.1.
    RefSeqiXP_828695.1. XM_823602.1.

    Genome annotation databases

    EnsemblProtistsiEAN79583; EAN79583; Tb11.02.4080.
    GeneIDi3665105.
    KEGGitbr:Tb11.02.4080.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH464491 Genomic DNA. Translation: EAN79583.1 .
    RefSeqi XP_828695.1. XM_823602.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2WV2 X-ray 2.70 A 22-481 [» ]
    2X2N X-ray 2.60 A/B/C/D 22-481 [» ]
    3G1Q X-ray 1.89 A/B/C/D 29-476 [» ]
    3GW9 X-ray 1.87 A/B/C/D 29-477 [» ]
    3P99 X-ray 3.00 A/B/C/D 29-481 [» ]
    3TIK X-ray 2.05 A/B/C/D 32-481 [» ]
    4BJK X-ray 2.67 A/B/C/D 32-481 [» ]
    4G3J X-ray 1.83 A/B/C/D 29-476 [» ]
    4G7G X-ray 2.05 A/B/C/D 29-476 [» ]
    ProteinModelPortali Q385E8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5691.Tb11.02.4080.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi EAN79583 ; EAN79583 ; Tb11.02.4080 .
    GeneIDi 3665105.
    KEGGi tbr:Tb11.02.4080.

    Phylogenomic databases

    eggNOGi COG2124.
    HOGENOMi HOG000042780.
    KOi K05917.

    Miscellaneous databases

    EvolutionaryTracei Q385E8.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002403. Cyt_P450_E_grp-IV.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00465. EP450IV.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of the African trypanosome Trypanosoma brucei."
      Berriman M., Ghedin E., Hertz-Fowler C., Blandin G., Renauld H., Bartholomeu D.C., Lennard N.J., Caler E., Hamlin N.E., Haas B., Bohme U., Hannick L., Aslett M.A., Shallom J., Marcello L., Hou L., Wickstead B., Alsmark U.C.M.
      , Arrowsmith C., Atkin R.J., Barron A.J., Bringaud F., Brooks K., Carrington M., Cherevach I., Chillingworth T.J., Churcher C., Clark L.N., Corton C.H., Cronin A., Davies R.M., Doggett J., Djikeng A., Feldblyum T., Field M.C., Fraser A., Goodhead I., Hance Z., Harper D., Harris B.R., Hauser H., Hostetler J., Ivens A., Jagels K., Johnson D., Johnson J., Jones K., Kerhornou A.X., Koo H., Larke N., Landfear S., Larkin C., Leech V., Line A., Lord A., Macleod A., Mooney P.J., Moule S., Martin D.M., Morgan G.W., Mungall K., Norbertczak H., Ormond D., Pai G., Peacock C.S., Peterson J., Quail M.A., Rabbinowitsch E., Rajandream M.A., Reitter C., Salzberg S.L., Sanders M., Schobel S., Sharp S., Simmonds M., Simpson A.J., Tallon L., Turner C.M., Tait A., Tivey A.R., Van Aken S., Walker D., Wanless D., Wang S., White B., White O., Whitehead S., Woodward J., Wortman J., Adams M.D., Embley T.M., Gull K., Ullu E., Barry J.D., Fairlamb A.H., Opperdoes F., Barrell B.G., Donelson J.E., Hall N., Fraser C.M., Melville S.E., El-Sayed N.M.A.
      Science 309:416-422(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 927/4 GUTat10.1Imported.
    2. "Crystal structures of Trypanosoma brucei sterol 14alpha-demethylase and implications for selective treatment of human infections."
      Lepesheva G.I., Park H.W., Hargrove T.Y., Vanhollebeke B., Wawrzak Z., Harp J.M., Sundaramoorthy M., Nes W.D., Pays E., Chaudhuri M., Villalta F., Waterman M.R.
      J. Biol. Chem. 285:1773-1780(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.87 ANGSTROMS) OF 29-477 IN COMPLEX WITH HEME.
    3. "Structural characterization of CYP51 from Trypanosoma cruzi and Trypanosoma brucei bound to the antifungal drugs posaconazole and fluconazole."
      Chen C.K., Leung S.S., Guilbert C., Jacobson M.P., McKerrow J.H., Podust L.M.
      PLoS Negl. Trop. Dis. 4:e651-e651(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS) OF 22-481 IN COMPLEX WITH HEME.
    4. "Pharmacological characterization, structural studies, and in vivo activities of anti-Chagas disease lead compounds derived from tipifarnib."
      Buckner F.S., Bahia M.T., Suryadevara P.K., White K.L., Shackleford D.M., Chennamaneni N.K., Hulverson M.A., Laydbak J.U., Chatelain E., Scandale I., Verlinde C.L., Charman S.A., Lepesheva G.I., Gelb M.H.
      Antimicrob. Agents Chemother. 56:4914-4921(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS) OF 32-481 IN COMPLEX WITH HEME.
    5. "Structural complex of sterol 14?-demethylase (CYP51) with 14?-methylenecyclopropyl-Delta7-24, 25-dihydrolanosterol."
      Hargrove T.Y., Wawrzak Z., Liu J., Waterman M.R., Nes W.D., Lepesheva G.I.
      J. Lipid Res. 53:311-320(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.00 ANGSTROMS) OF 29-481 IN COMPLEX WITH HEME.
    6. "CYP51 structure-based VNI scaffold development."
      Hargrove T.Y., Wawrzak Z., Waterman M.R., Lepesheva G.I.
      Submitted (JUL-2012) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (1.83 ANGSTROMS) OF 29-476 IN COMPLEX WITH HEME.
    7. "Rational development of 4-aminopyridyl-based inhibitors targeting Trypanosoma cruzi CYP51 as anti-chagas agents."
      Choi J.Y., Calvet C.M., Gunatilleke S.S., Ruiz C., Cameron M.D., McKerrow J.H., Podust L.M., Roush W.R.
      J. Med. Chem. 56:7651-7668(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.67 ANGSTROMS) OF 32-481 IN COMPLEX WITH HEME.

    Entry informationi

    Entry nameiQ385E8_TRYB2
    AccessioniPrimary (citable) accession number: Q385E8
    Entry historyi
    Integrated into UniProtKB/TrEMBL: November 22, 2005
    Last sequence update: November 22, 2005
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported, Complete proteome, Reference proteomeImported

    External Data

    Dasty 3