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Q38100 (RUSA_BPR1T) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Crossover junction endodeoxyribonuclease rusA

EC=3.1.22.4
Alternative name(s):
Holliday junction nuclease rusA
Holliday junction resolvase
Gene names
Name:rusA
ORF Names:ORF14
OrganismLactococcus phage r1t (Bacteriophage r1t) [Complete proteome]
Taxonomic identifier43685 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageCaudoviralesSiphoviridae
Virus hostLactococcus lactis subsp. cremoris (Streptococcus cremoris) [TaxID: 1359]

Protein attributes

Sequence length131 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Endonuclease that resolves Holliday junction intermediates. Promotes DNA repair. Exhibits sequence and structure-selective cleavage of four-way DNA junctions, where it introduces symmetrical nicks in two strands of the same polarity, though it can also cut branched DNA structures that lack the symmetry of Holliday junctions. Ref.3

Catalytic activity

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).

Cofactor

Binds 1 magnesium ion per subunit By similarity.

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the RusA family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 131131Crossover junction endodeoxyribonuclease rusA
PRO_0000324839

Sites

Metal binding851Magnesium By similarity
Metal binding871Magnesium By similarity
Metal binding1061Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
Q38100 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 9245E387434D12A1

FASTA13116,001
        10         20         30         40         50         60 
MKFEFEFELD KMPTTQQQKG IKKVKGKLQF YDRRGTNNYS LKAQLMKNKP KECFEKNVPL 

        70         80         90        100        110        120 
KLSVTFFYAI KQKKRWWQWK TSRPDLDNLM KNLQDYMTKL RYYSDDSQIV WLEAKKVNDE 

       130 
KNRIEIEITE V 

« Hide

References

[1]"Inducible gene expression mediated by a repressor-operator system isolated from Lactococcus lactis bacteriophage r1t."
Nauta A., van Sinderen D., Karsens H., Smit E., Venema G., Kok J.
Mol. Microbiol. 19:1331-1341(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Sequence analysis and molecular characterization of the temperate lactococcal bacteriophage r1t."
van Sinderen D., Karsens H., Kok J., Terpstra P., Ruiters M.H., Venema G., Nauta A.
Mol. Microbiol. 19:1343-1355(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"RusA proteins from the extreme thermophile Aquifex aeolicus and lactococcal phage r1t resolve Holliday junctions."
Sharples G.J., Bolt E.L., Lloyd R.G.
Mol. Microbiol. 44:549-559(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U38906 Genomic DNA. Translation: AAB18689.1.
RefSeqNP_695042.1. NC_004302.1.

3D structure databases

ProteinModelPortalQ38100.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID955502.

Phylogenomic databases

ProtClustDBCLSP2343499.

Family and domain databases

Gene3D3.30.1330.70. 1 hit.
InterProIPR008822. Endonuclease_RusA-like.
[Graphical view]
PfamPF05866. RusA. 1 hit.
[Graphical view]
SUPFAMSSF103084. SSF103084. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRUSA_BPR1T
AccessionPrimary (citable) accession number: Q38100
Entry history
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families