Q38100 (RUSA_BPR1T) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Crossover junction endodeoxyribonuclease rusA
EC=3.1.22.4

Alternative name(s):
Holliday junction nuclease rusA
Holliday junction resolvase

Gene names

Name:	rusA
ORF Names:	ORF14

Organism

Lactococcus phage r1t (Bacteriophage r1t) [Complete proteome]

Taxonomic identifier

43685 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Caudovirales › Siphoviridae › unclassified Siphoviridae

Virus host

Lactococcus lactis subsp. cremoris (Streptococcus cremoris) [TaxID: 1359]

Protein attributes

Sequence length	131 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Endonuclease that resolves Holliday junction intermediates. Promotes DNA repair. Exhibits sequence and structure-selective cleavage of four-way DNA junctions, where it introduces symmetrical nicks in two strands of the same polarity, though it can also cut branched DNA structures that lack the symmetry of Holliday junctions. Ref.3
Catalytic activity	Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the RusA family.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair
Ligand	Magnesium Metal-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA repair Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	crossover junction endodeoxyribonuclease activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 131	131	Crossover junction endodeoxyribonuclease rusA		PRO_0000324839
Sites
	Metal binding	85	1	Magnesium By similarity
	Metal binding	87	1	Magnesium By similarity
	Metal binding	106	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q38100 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 9245E387434D12A1	FASTA	131	16,001
	10 20 30 40 50 60 MKFEFEFELD KMPTTQQQKG IKKVKGKLQF YDRRGTNNYS LKAQLMKNKP KECFEKNVPL 70 80 90 100 110 120 KLSVTFFYAI KQKKRWWQWK TSRPDLDNLM KNLQDYMTKL RYYSDDSQIV WLEAKKVNDE 130 KNRIEIEITE V

References

[1]	"Inducible gene expression mediated by a repressor-operator system isolated from Lactococcus lactis bacteriophage r1t." Nauta A., van Sinderen D., Karsens H., Smit E., Venema G., Kok J. Mol. Microbiol. 19:1331-1341(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence analysis and molecular characterization of the temperate lactococcal bacteriophage r1t." van Sinderen D., Karsens H., Kok J., Terpstra P., Ruiters M.H., Venema G., Nauta A. Mol. Microbiol. 19:1343-1355(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"RusA proteins from the extreme thermophile Aquifex aeolicus and lactococcal phage r1t resolve Holliday junctions." Sharples G.J., Bolt E.L., Lloyd R.G. Mol. Microbiol. 44:549-559(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U38906 Genomic DNA. Translation: AAB18689.1.
RefSeq	NP_695042.1. NC_004302.1.
3D structure databases
ProteinModelPortal	Q38100.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	955502.
Phylogenomic databases
ProtClustDB	CLSP2343499.
Family and domain databases
Gene3D	3.30.1330.70. 1 hit.
InterPro	IPR008822. Endonuclease_RusA-like. [Graphical view]
Pfam	PF05866. RusA. 1 hit. [Graphical view]
SUPFAM	SSF103084. RusA-like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RUSA_BPR1T

Accession

Primary (citable) accession number: Q38100

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program