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Q38100

- RUSA_BPR1T

UniProt

Q38100 - RUSA_BPR1T

Protein

Crossover junction endodeoxyribonuclease rusA

Gene

rusA

Organism
Lactococcus phage r1t (Bacteriophage r1t)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
  1. Functioni

    Endonuclease that resolves Holliday junction intermediates. Promotes DNA repair. Exhibits sequence and structure-selective cleavage of four-way DNA junctions, where it introduces symmetrical nicks in two strands of the same polarity, though it can also cut branched DNA structures that lack the symmetry of Holliday junctions.1 Publication

    Catalytic activityi

    Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi85 – 851MagnesiumBy similarity
    Metal bindingi87 – 871MagnesiumBy similarity
    Metal bindingi106 – 1061MagnesiumBy similarity

    GO - Molecular functioni

    1. crossover junction endodeoxyribonuclease activity Source: UniProtKB-EC
    2. magnesium ion binding Source: InterPro

    GO - Biological processi

    1. DNA recombination Source: UniProtKB-KW
    2. DNA repair Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    DNA damage, DNA recombination, DNA repair

    Keywords - Ligandi

    Magnesium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Crossover junction endodeoxyribonuclease rusA (EC:3.1.22.4)
    Alternative name(s):
    Holliday junction nuclease rusA
    Holliday junction resolvase
    Gene namesi
    Name:rusA
    ORF Names:ORF14
    OrganismiLactococcus phage r1t (Bacteriophage r1t)
    Taxonomic identifieri43685 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesSiphoviridae
    Virus hostiLactococcus lactis subsp. cremoris (Streptococcus cremoris) [TaxID: 1359]
    ProteomesiUP000001164: Genome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 131131Crossover junction endodeoxyribonuclease rusAPRO_0000324839Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ38100.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the RusA family.Curated

    Family and domain databases

    Gene3Di3.30.1330.70. 1 hit.
    InterProiIPR008822. Endonuclease_RusA-like.
    [Graphical view]
    PfamiPF05866. RusA. 1 hit.
    [Graphical view]
    SUPFAMiSSF103084. SSF103084. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q38100-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKFEFEFELD KMPTTQQQKG IKKVKGKLQF YDRRGTNNYS LKAQLMKNKP    50
    KECFEKNVPL KLSVTFFYAI KQKKRWWQWK TSRPDLDNLM KNLQDYMTKL 100
    RYYSDDSQIV WLEAKKVNDE KNRIEIEITE V 131
    Length:131
    Mass (Da):16,001
    Last modified:November 1, 1996 - v1
    Checksum:i9245E387434D12A1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U38906 Genomic DNA. Translation: AAB18689.1.
    RefSeqiNP_695042.1. NC_004302.1.

    Genome annotation databases

    GeneIDi955502.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U38906 Genomic DNA. Translation: AAB18689.1 .
    RefSeqi NP_695042.1. NC_004302.1.

    3D structure databases

    ProteinModelPortali Q38100.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 955502.

    Family and domain databases

    Gene3Di 3.30.1330.70. 1 hit.
    InterProi IPR008822. Endonuclease_RusA-like.
    [Graphical view ]
    Pfami PF05866. RusA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF103084. SSF103084. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Inducible gene expression mediated by a repressor-operator system isolated from Lactococcus lactis bacteriophage r1t."
      Nauta A., van Sinderen D., Karsens H., Smit E., Venema G., Kok J.
      Mol. Microbiol. 19:1331-1341(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Sequence analysis and molecular characterization of the temperate lactococcal bacteriophage r1t."
      van Sinderen D., Karsens H., Kok J., Terpstra P., Ruiters M.H., Venema G., Nauta A.
      Mol. Microbiol. 19:1343-1355(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "RusA proteins from the extreme thermophile Aquifex aeolicus and lactococcal phage r1t resolve Holliday junctions."
      Sharples G.J., Bolt E.L., Lloyd R.G.
      Mol. Microbiol. 44:549-559(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiRUSA_BPR1T
    AccessioniPrimary (citable) accession number: Q38100
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 18, 2008
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3