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Q38100

- RUSA_BPR1T

UniProt

Q38100 - RUSA_BPR1T

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Protein
Crossover junction endodeoxyribonuclease rusA
Gene
rusA, ORF14
Organism
Lactococcus phage r1t (Bacteriophage r1t)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Endonuclease that resolves Holliday junction intermediates. Promotes DNA repair. Exhibits sequence and structure-selective cleavage of four-way DNA junctions, where it introduces symmetrical nicks in two strands of the same polarity, though it can also cut branched DNA structures that lack the symmetry of Holliday junctions.1 Publication

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).

Cofactori

Binds 1 magnesium ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi85 – 851Magnesium By similarity
Metal bindingi87 – 871Magnesium By similarity
Metal bindingi106 – 1061Magnesium By similarity

GO - Molecular functioni

  1. crossover junction endodeoxyribonuclease activity Source: UniProtKB-EC
  2. magnesium ion binding Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. DNA recombination Source: UniProtKB-KW
  2. DNA repair Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endodeoxyribonuclease rusA (EC:3.1.22.4)
Alternative name(s):
Holliday junction nuclease rusA
Holliday junction resolvase
Gene namesi
Name:rusA
ORF Names:ORF14
OrganismiLactococcus phage r1t (Bacteriophage r1t)
Taxonomic identifieri43685 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesSiphoviridae
Virus hostiLactococcus lactis subsp. cremoris (Streptococcus cremoris) [TaxID: 1359]
ProteomesiUP000001164: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 131131Crossover junction endodeoxyribonuclease rusA
PRO_0000324839Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ38100.

Family & Domainsi

Sequence similaritiesi

Belongs to the RusA family.

Family and domain databases

Gene3Di3.30.1330.70. 1 hit.
InterProiIPR008822. Endonuclease_RusA-like.
[Graphical view]
PfamiPF05866. RusA. 1 hit.
[Graphical view]
SUPFAMiSSF103084. SSF103084. 1 hit.

Sequencei

Sequence statusi: Complete.

Q38100-1 [UniParc]FASTAAdd to Basket

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MKFEFEFELD KMPTTQQQKG IKKVKGKLQF YDRRGTNNYS LKAQLMKNKP    50
KECFEKNVPL KLSVTFFYAI KQKKRWWQWK TSRPDLDNLM KNLQDYMTKL 100
RYYSDDSQIV WLEAKKVNDE KNRIEIEITE V 131
Length:131
Mass (Da):16,001
Last modified:November 1, 1996 - v1
Checksum:i9245E387434D12A1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U38906 Genomic DNA. Translation: AAB18689.1.
RefSeqiNP_695042.1. NC_004302.1.

Genome annotation databases

GeneIDi955502.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U38906 Genomic DNA. Translation: AAB18689.1 .
RefSeqi NP_695042.1. NC_004302.1.

3D structure databases

ProteinModelPortali Q38100.
ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 955502.

Family and domain databases

Gene3Di 3.30.1330.70. 1 hit.
InterProi IPR008822. Endonuclease_RusA-like.
[Graphical view ]
Pfami PF05866. RusA. 1 hit.
[Graphical view ]
SUPFAMi SSF103084. SSF103084. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Inducible gene expression mediated by a repressor-operator system isolated from Lactococcus lactis bacteriophage r1t."
    Nauta A., van Sinderen D., Karsens H., Smit E., Venema G., Kok J.
    Mol. Microbiol. 19:1331-1341(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequence analysis and molecular characterization of the temperate lactococcal bacteriophage r1t."
    van Sinderen D., Karsens H., Kok J., Terpstra P., Ruiters M.H., Venema G., Nauta A.
    Mol. Microbiol. 19:1343-1355(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "RusA proteins from the extreme thermophile Aquifex aeolicus and lactococcal phage r1t resolve Holliday junctions."
    Sharples G.J., Bolt E.L., Lloyd R.G.
    Mol. Microbiol. 44:549-559(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiRUSA_BPR1T
AccessioniPrimary (citable) accession number: Q38100
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: November 1, 1996
Last modified: May 14, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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