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Q37873 (RUSA_BP82) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Crossover junction endodeoxyribonuclease rusA

EC=3.1.22.4
Alternative name(s):
Holliday junction nuclease rusA
Holliday junction resolvase
Gene names
Name:rusA
Synonyms:rus
OrganismEnterobacteria phage 82 (Bacteriophage 82)
Taxonomic identifier10705 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageCaudoviralesSiphoviridaeLambdalikevirusunclassified Lambda-like viruses
Virus hostEscherichia coli [TaxID: 562]

Protein attributes

Sequence length120 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Endonuclease that resolves Holliday junction intermediates. Promotes DNA repair. Exhibits sequence and structure-selective cleavage of four-way DNA junctions, where it introduces symmetrical nicks in two strands of the same polarity.

Catalytic activity

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).

Cofactor

Binds 1 magnesium ion per subunit By similarity.

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the RusA family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 120120Crossover junction endodeoxyribonuclease rusA
PRO_0000192006

Sites

Metal binding701Magnesium By similarity
Metal binding721Magnesium By similarity
Metal binding911Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
Q37873 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 5E70726964490A7F

FASTA12013,885
        10         20         30         40         50         60 
MNTYHITLPW PPSNNRYYRH NRGRTHISAE GQAYRDNVAR IIKGSMLDIG LAMPVKIRIE 

        70         80         90        100        110        120 
CHMPDRRRRD LDNLQKASFD ALTKAGFWLD DAQVVDYRVV KMPVTKGGRL ELTITEMGNE 

« Hide

References

[1]"Holliday junction resolvases encoded by homologous rusA genes in Escherichia coli K-12 and phage 82."
Mahdi A.A., Sharples G.J., Mandal T.N., Lloyd R.G.
J. Mol. Biol. 257:561-573(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X92588 Genomic DNA. Translation: CAA63330.1.
PIRS66583.

3D structure databases

ProteinModelPortalQ37873.
SMRQ37873. Positions 1-120.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.30.1330.70. 1 hit.
InterProIPR016281. Endonuclease_RusA.
IPR008822. Endonuclease_RusA-like.
[Graphical view]
PfamPF05866. RusA. 1 hit.
[Graphical view]
PIRSFPIRSF001007. RusA. 1 hit.
SUPFAMSSF103084. SSF103084. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRUSA_BP82
AccessionPrimary (citable) accession number: Q37873
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families