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Q37873

- RUSA_BP82

UniProt

Q37873 - RUSA_BP82

Protein

Crossover junction endodeoxyribonuclease rusA

Gene

rusA

Organism
Enterobacteria phage 82 (Bacteriophage 82)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
  1. Functioni

    Endonuclease that resolves Holliday junction intermediates. Promotes DNA repair. Exhibits sequence and structure-selective cleavage of four-way DNA junctions, where it introduces symmetrical nicks in two strands of the same polarity.

    Catalytic activityi

    Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi70 – 701MagnesiumBy similarity
    Metal bindingi72 – 721MagnesiumBy similarity
    Metal bindingi91 – 911MagnesiumBy similarity

    GO - Molecular functioni

    1. crossover junction endodeoxyribonuclease activity Source: UniProtKB-EC
    2. magnesium ion binding Source: InterPro

    GO - Biological processi

    1. DNA recombination Source: UniProtKB-KW
    2. DNA repair Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    DNA damage, DNA recombination, DNA repair

    Keywords - Ligandi

    Magnesium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Crossover junction endodeoxyribonuclease rusA (EC:3.1.22.4)
    Alternative name(s):
    Holliday junction nuclease rusA
    Holliday junction resolvase
    Gene namesi
    Name:rusA
    Synonyms:rus
    OrganismiEnterobacteria phage 82 (Bacteriophage 82)
    Taxonomic identifieri10705 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesSiphoviridaeLambdalikevirusunclassified Lambda-like viruses
    Virus hostiEscherichia coli [TaxID: 562]

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 120120Crossover junction endodeoxyribonuclease rusAPRO_0000192006Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ37873.
    SMRiQ37873. Positions 1-120.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the RusA family.Curated

    Family and domain databases

    Gene3Di3.30.1330.70. 1 hit.
    InterProiIPR016281. Endonuclease_RusA.
    IPR008822. Endonuclease_RusA-like.
    [Graphical view]
    PfamiPF05866. RusA. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001007. RusA. 1 hit.
    SUPFAMiSSF103084. SSF103084. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q37873-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNTYHITLPW PPSNNRYYRH NRGRTHISAE GQAYRDNVAR IIKGSMLDIG    50
    LAMPVKIRIE CHMPDRRRRD LDNLQKASFD ALTKAGFWLD DAQVVDYRVV 100
    KMPVTKGGRL ELTITEMGNE 120
    Length:120
    Mass (Da):13,885
    Last modified:November 1, 1996 - v1
    Checksum:i5E70726964490A7F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X92588 Genomic DNA. Translation: CAA63330.1.
    PIRiS66583.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X92588 Genomic DNA. Translation: CAA63330.1 .
    PIRi S66583.

    3D structure databases

    ProteinModelPortali Q37873.
    SMRi Q37873. Positions 1-120.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.30.1330.70. 1 hit.
    InterProi IPR016281. Endonuclease_RusA.
    IPR008822. Endonuclease_RusA-like.
    [Graphical view ]
    Pfami PF05866. RusA. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001007. RusA. 1 hit.
    SUPFAMi SSF103084. SSF103084. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Holliday junction resolvases encoded by homologous rusA genes in Escherichia coli K-12 and phage 82."
      Mahdi A.A., Sharples G.J., Mandal T.N., Lloyd R.G.
      J. Mol. Biol. 257:561-573(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiRUSA_BP82
    AccessioniPrimary (citable) accession number: Q37873
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 58 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3