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Q37873

- RUSA_BP82

UniProt

Q37873 - RUSA_BP82

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Protein

Crossover junction endodeoxyribonuclease rusA

Gene

rusA

Organism
Enterobacteria phage 82 (Bacteriophage 82)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Endonuclease that resolves Holliday junction intermediates. Promotes DNA repair. Exhibits sequence and structure-selective cleavage of four-way DNA junctions, where it introduces symmetrical nicks in two strands of the same polarity.

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).

Cofactori

Mg2+By similarityNote: Binds 1 Mg(2+) ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi70 – 701MagnesiumBy similarity
Metal bindingi72 – 721MagnesiumBy similarity
Metal bindingi91 – 911MagnesiumBy similarity

GO - Molecular functioni

  1. crossover junction endodeoxyribonuclease activity Source: UniProtKB-EC
  2. magnesium ion binding Source: InterPro

GO - Biological processi

  1. DNA recombination Source: UniProtKB-KW
  2. DNA repair Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endodeoxyribonuclease rusA (EC:3.1.22.4)
Alternative name(s):
Holliday junction nuclease rusA
Holliday junction resolvase
Gene namesi
Name:rusA
Synonyms:rus
OrganismiEnterobacteria phage 82 (Bacteriophage 82)
Taxonomic identifieri10705 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesSiphoviridaeLambdalikevirusunclassified Lambda-like viruses
Virus hostiEscherichia coli [TaxID: 562]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 120120Crossover junction endodeoxyribonuclease rusAPRO_0000192006Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ37873.
SMRiQ37873. Positions 1-120.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RusA family.Curated

Family and domain databases

Gene3Di3.30.1330.70. 1 hit.
InterProiIPR016281. Endonuclease_RusA.
IPR008822. Endonuclease_RusA-like.
[Graphical view]
PfamiPF05866. RusA. 1 hit.
[Graphical view]
PIRSFiPIRSF001007. RusA. 1 hit.
SUPFAMiSSF103084. SSF103084. 1 hit.

Sequencei

Sequence statusi: Complete.

Q37873-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNTYHITLPW PPSNNRYYRH NRGRTHISAE GQAYRDNVAR IIKGSMLDIG
60 70 80 90 100
LAMPVKIRIE CHMPDRRRRD LDNLQKASFD ALTKAGFWLD DAQVVDYRVV
110 120
KMPVTKGGRL ELTITEMGNE
Length:120
Mass (Da):13,885
Last modified:November 1, 1996 - v1
Checksum:i5E70726964490A7F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92588 Genomic DNA. Translation: CAA63330.1.
PIRiS66583.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92588 Genomic DNA. Translation: CAA63330.1 .
PIRi S66583.

3D structure databases

ProteinModelPortali Q37873.
SMRi Q37873. Positions 1-120.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.30.1330.70. 1 hit.
InterProi IPR016281. Endonuclease_RusA.
IPR008822. Endonuclease_RusA-like.
[Graphical view ]
Pfami PF05866. RusA. 1 hit.
[Graphical view ]
PIRSFi PIRSF001007. RusA. 1 hit.
SUPFAMi SSF103084. SSF103084. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Holliday junction resolvases encoded by homologous rusA genes in Escherichia coli K-12 and phage 82."
    Mahdi A.A., Sharples G.J., Mandal T.N., Lloyd R.G.
    J. Mol. Biol. 257:561-573(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiRUSA_BP82
AccessioniPrimary (citable) accession number: Q37873
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 26, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3