Q37873 (RUSA_BP82) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Crossover junction endodeoxyribonuclease rusA
EC=3.1.22.4

Alternative name(s):
Holliday junction nuclease rusA
Holliday junction resolvase

Gene names

Name:	rusA
Synonyms:	rus

Organism

Enterobacteria phage 82 (Bacteriophage 82)

Taxonomic identifier

10705 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Caudovirales › Siphoviridae › Lambdalikevirus › unclassified Lambda-like viruses

Virus host

Escherichia coli [TaxID: 562]

Protein attributes

Sequence length	120 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Endonuclease that resolves Holliday junction intermediates. Promotes DNA repair. Exhibits sequence and structure-selective cleavage of four-way DNA junctions, where it introduces symmetrical nicks in two strands of the same polarity.
Catalytic activity	Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the RusA family.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair
Ligand	Magnesium Metal-binding
Molecular function	Endonuclease Hydrolase Nuclease
Gene Ontology (GO)
Biological_process	DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA repair Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	crossover junction endodeoxyribonuclease activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 120	120	Crossover junction endodeoxyribonuclease rusA		PRO_0000192006
Sites
	Metal binding	70	1	Magnesium By similarity
	Metal binding	72	1	Magnesium By similarity
	Metal binding	91	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q37873 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 5E70726964490A7F	FASTA	120	13,885
	10 20 30 40 50 60 MNTYHITLPW PPSNNRYYRH NRGRTHISAE GQAYRDNVAR IIKGSMLDIG LAMPVKIRIE 70 80 90 100 110 120 CHMPDRRRRD LDNLQKASFD ALTKAGFWLD DAQVVDYRVV KMPVTKGGRL ELTITEMGNE

References

[1]	"Holliday junction resolvases encoded by homologous rusA genes in Escherichia coli K-12 and phage 82." Mahdi A.A., Sharples G.J., Mandal T.N., Lloyd R.G. J. Mol. Biol. 257:561-573(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X92588 Genomic DNA. Translation: CAA63330.1.
PIR	S66583.
3D structure databases
ProteinModelPortal	Q37873.
SMR	Q37873. Positions 1-120.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.30.1330.70. 1 hit.
InterPro	IPR016281. Endonuclease_RusA. IPR008822. Endonuclease_RusA-like. [Graphical view]
Pfam	PF05866. RusA. 1 hit. [Graphical view]
PIRSF	PIRSF001007. RusA. 1 hit.
SUPFAM	SSF103084. RusA-like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RUSA_BP82

Accession

Primary (citable) accession number: Q37873

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program