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Protein

NADH-ubiquinone oxidoreductase 49 kDa subunit

Gene

NAD7

Organism
Acanthamoeba castellanii (Amoeba)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). Component of the iron-sulfur (IP) fragment of the enzyme.By similarity

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

GO - Molecular functioni

  1. NAD binding Source: InterPro
  2. NADH dehydrogenase (ubiquinone) activity Source: UniProtKB-EC
  3. quinone binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

NAD, Ubiquinone

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-ubiquinone oxidoreductase 49 kDa subunit (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
NADH dehydrogenase subunit 7
Gene namesi
Name:NAD7
Encoded oniMitochondrion
OrganismiAcanthamoeba castellanii (Amoeba)
Taxonomic identifieri5755 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaDiscoseaLongamoebiaCentramoebidaAcanthamoebidaeAcanthamoeba

Subcellular locationi

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-SubCell
  2. respiratory chain Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 401401NADH-ubiquinone oxidoreductase 49 kDa subunitPRO_0000118587Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ37384.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I 49 kDa subunit family.Curated

Family and domain databases

Gene3Di1.10.645.10. 1 hit.
HAMAPiMF_01358. NDH1_NuoD.
InterProiIPR001135. NADH_Q_OxRdtase_suD.
IPR014029. NADH_UbQ_OxRdtase_49kDa_CS.
IPR022885. NDH1_su_D/H.
IPR029014. NiFe_Hase-like.
[Graphical view]
PfamiPF00346. Complex1_49kDa. 1 hit.
[Graphical view]
SUPFAMiSSF56762. SSF56762. 1 hit.
TIGRFAMsiTIGR01962. NuoD. 1 hit.
PROSITEiPS00535. COMPLEX1_49K. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q37384-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKNQISPYI KTSKIKNFTM NFGPQHPAAH GVLRLILELD GELVRKADPH
60 70 80 90 100
IGLLHRGTEK LIEYKTYIQA LPYFDRLDYV SMMSQEHAYS LAIEKLLNCN
110 120 130 140 150
VPIRAQYIRV IYSELTRILN HILAVTTHAM DVGALTPFLW LFEEREKLME
160 170 180 190 200
FYERVSGARM HAAYIRPGGV AQDFPLGLWN DIMQFVEQFF WRLVEVEELL
210 220 230 240 250
NGNRIWKQRL VDVGIVTAEE ALSHGFSGVM LRGSGIAWDL RKNNPYEVYN
260 270 280 290 300
KLNFNIPIGK NGDCYDRYLI RVYEMYESLN IIKQCLTAMP AGLIKVNDKK
310 320 330 340 350
ITPPERTDMK YSMESLIHHF KLYSEGFNVP ENETYACVEA PKGEFGVYVV
360 370 380 390 400
SDGSNKPYRC KIKAPGFLHL QSLNSMSKGH MIADVVTIIG TQDIVFGEID

R
Length:401
Mass (Da):46,096
Last modified:November 1, 1996 - v1
Checksum:iBA929F88024C0DF8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12386 Genomic DNA. Translation: AAD11854.1.
PIRiS53862.
RefSeqiNP_042561.1. NC_001637.1.

Genome annotation databases

GeneIDi1734056.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12386 Genomic DNA. Translation: AAD11854.1.
PIRiS53862.
RefSeqiNP_042561.1. NC_001637.1.

3D structure databases

ProteinModelPortaliQ37384.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1734056.

Family and domain databases

Gene3Di1.10.645.10. 1 hit.
HAMAPiMF_01358. NDH1_NuoD.
InterProiIPR001135. NADH_Q_OxRdtase_suD.
IPR014029. NADH_UbQ_OxRdtase_49kDa_CS.
IPR022885. NDH1_su_D/H.
IPR029014. NiFe_Hase-like.
[Graphical view]
PfamiPF00346. Complex1_49kDa. 1 hit.
[Graphical view]
SUPFAMiSSF56762. SSF56762. 1 hit.
TIGRFAMsiTIGR01962. NuoD. 1 hit.
PROSITEiPS00535. COMPLEX1_49K. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The mitochondrial DNA of the amoeboid protozoon, Acanthamoeba castellanii: complete sequence, gene content and genome organization."
    Burger G., Plante I., Lonergan K.M., Gray M.W.
    J. Mol. Biol. 245:522-537(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 30010 / Neff.

Entry informationi

Entry nameiNDUS2_ACACA
AccessioniPrimary (citable) accession number: Q37384
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: January 7, 2015
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.