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Q37373

- NDUS1_ACACA

UniProt

Q37373 - NDUS1_ACACA

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Protein

NADH-ubiquinone oxidoreductase 75 kDa subunit

Gene

NAD11

Organism
Acanthamoeba castellanii (Amoeba)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized (By similarity).By similarity

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

Protein has several cofactor binding sites:
  • [2Fe-2S] clusterBy similarityNote: Binds 1 [2Fe-2S] cluster per subunit.By similarity
  • [4Fe-4S] clusterBy similarityNote: Binds 2 [4Fe-4S] clusters per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi36 – 361Iron-sulfur 1 (2Fe-2S)By similarity
Metal bindingi47 – 471Iron-sulfur 1 (2Fe-2S)By similarity
Metal bindingi50 – 501Iron-sulfur 1 (2Fe-2S)By similarity
Metal bindingi64 – 641Iron-sulfur 1 (2Fe-2S)By similarity
Metal bindingi96 – 961Iron-sulfur 2 (4Fe-4S); via pros nitrogenBy similarity
Metal bindingi100 – 1001Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi103 – 1031Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi109 – 1091Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi148 – 1481Iron-sulfur 3 (4Fe-4S)By similarity
Metal bindingi151 – 1511Iron-sulfur 3 (4Fe-4S)By similarity
Metal bindingi154 – 1541Iron-sulfur 3 (4Fe-4S)By similarity
Metal bindingi198 – 1981Iron-sulfur 3 (4Fe-4S)By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. electron carrier activity Source: InterPro
  4. metal ion binding Source: UniProtKB-KW
  5. NADH dehydrogenase (ubiquinone) activity Source: UniProtKB-EC

GO - Biological processi

  1. ATP synthesis coupled electron transport Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-ubiquinone oxidoreductase 75 kDa subunit (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
Complex I-75kD
Short name:
CI-75kD
NADH dehydrogenase subunit 11
Gene namesi
Name:NAD11
Encoded oniMitochondrion
OrganismiAcanthamoeba castellanii (Amoeba)
Taxonomic identifieri5755 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaDiscoseaLongamoebiaCentramoebidaAcanthamoebidaeAcanthamoeba

Subcellular locationi

Mitochondrion inner membrane By similarity
Note: Matrix and cytoplasmic side of the mitochondrial inner membrane.By similarity

GO - Cellular componenti

  1. mitochondrial inner membrane Source: UniProtKB-KW
  2. respiratory chain Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 675675NADH-ubiquinone oxidoreductase 75 kDa subunitPRO_0000118536Add
BLAST

Interactioni

Subunit structurei

Complex I is composed of about 30 different subunits.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ37373.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 80792Fe-2S ferredoxin-typeAdd
BLAST
Domaini217 – 273574Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the complex I 75 kDa subunit family.Curated
Contains 1 4Fe-4S Mo/W bis-MGD-type domain.PROSITE-ProRule annotation

Family and domain databases

InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR000283. NADH_UbQ_OxRdtase_75kDa_su_CS.
IPR010228. NADH_UbQ_OxRdtase_Gsu.
IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd.
[Graphical view]
PfamiPF00384. Molybdopterin. 2 hits.
PF10588. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view]
SMARTiSM00929. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view]
SUPFAMiSSF54292. SSF54292. 1 hit.
TIGRFAMsiTIGR01973. NuoG. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00642. COMPLEX1_75K_2. 1 hit.
PS00643. COMPLEX1_75K_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q37373-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKNISFKVND FQYTINNKLT LIQACLKNKV DISRFCFHEK LSIAGNCRMC
60 70 80 90 100
LVEDLKQVKP LASCAINVSN SMNIYTNTLK VKKARESVLE FLLANHPLDC
110 120 130 140 150
PICDQGGECD LQDQSVVFGS DRGRFYEFKR SVEDKDCGPL IKTIMNRCIH
160 170 180 190 200
CTRCVRFSNE VAGVNILGVT GRGSKMEIGF YIENLMRSEL SGNVIDLCPV
210 220 230 240 250
GALTSKPFAF TSRPWELKSY NSIDVLDSLH SNIRVDIRGT KIMRILPRVN
260 270 280 290 300
SELNEDWITD KIRFSYDSFR RQRLYDPMVK ISGSFLKIGW KKAMLFIKKF
310 320 330 340 350
FCNFLGFNHS SFIPLRGYIG DYLDLETIYT FKKFLLLNGS NFFLPSSSYN
360 370 380 390 400
DLTALYSFNT PLTRLDEGDF CILLDVNLRV ELPIVNSRIK QLVSKKMLPV
410 420 430 440 450
FVLGFYSNFN YFVKHISNSS KTLLHVLEGS HWLSAKISKK FSSKPIFLIG
460 470 480 490 500
DSSSLLKGSL IVPLFNFTNV ICDNWNGLNI ISNDSSYLST KEFNLSSSHS
510 520 530 540 550
QNSHLLNFPI NFVLNYDKAV LVDSSAFQIY QGHHGDTNAI NSNLIFPSTS
560 570 580 590 600
FIEKNSFYSN SLAIVQKTKK ILFSPGNSRD DWKILNALID NFGFSYFKVR
610 620 630 640 650
NSFDLVSFLS ESTPFILYKR SFSFKCFGFY EQLVYHFFNY FSVNNNYYIY
660 670
DSITRNSKIM SLCFNKFKMK GYNFF
Length:675
Mass (Da):77,554
Last modified:November 1, 1996 - v1
Checksum:i3870F3BC9E8B9807
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12386 Genomic DNA. Translation: AAD11824.1.
PIRiS53832.
RefSeqiNP_042531.1. NC_001637.1.

Genome annotation databases

GeneIDi1734026.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12386 Genomic DNA. Translation: AAD11824.1 .
PIRi S53832.
RefSeqi NP_042531.1. NC_001637.1.

3D structure databases

ProteinModelPortali Q37373.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1734026.

Family and domain databases

InterProi IPR001041. 2Fe-2S_ferredoxin-type.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR000283. NADH_UbQ_OxRdtase_75kDa_su_CS.
IPR010228. NADH_UbQ_OxRdtase_Gsu.
IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd.
[Graphical view ]
Pfami PF00384. Molybdopterin. 2 hits.
PF10588. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view ]
SMARTi SM00929. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view ]
SUPFAMi SSF54292. SSF54292. 1 hit.
TIGRFAMsi TIGR01973. NuoG. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00642. COMPLEX1_75K_2. 1 hit.
PS00643. COMPLEX1_75K_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The mitochondrial DNA of the amoeboid protozoon, Acanthamoeba castellanii: complete sequence, gene content and genome organization."
    Burger G., Plante I., Lonergan K.M., Gray M.W.
    J. Mol. Biol. 245:522-537(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 30010 / Neff.

Entry informationi

Entry nameiNDUS1_ACACA
AccessioniPrimary (citable) accession number: Q37373
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: November 26, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3