Reviewed, UniProtKB/Swiss-Prot Q37373 (NDUS1_ACACA)

Names and origin

Protein names

Recommended name:
    NADH-ubiquinone oxidoreductase 75 kDa subunit
    EC=1.6.5.3
    EC=1.6.99.3
Alternative name(s):
    Complex I-75kD
      Short name=CI-75kD
    NADH dehydrogenase subunit 11

Gene names

Name:

NAD11

Encoded on

Mitochondrion

Organism

Acanthamoeba castellanii (Amoeba)

Taxonomic identifier

5755 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Centramoebida › Acanthamoebidae › Acanthamoeba

Protein attributes

Sequence length	675 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity. This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized By similarity.
Catalytic activity	NADH + ubiquinone = NAD(+) + ubiquinol. NADH + acceptor = NAD(+) + reduced acceptor.
Cofactor	Binds 1 2Fe-2S cluster per subunit By similarity. Binds 2 4Fe-4S clusters per subunit By similarity.
Subunit structure	Complex I is composed of about 30 different subunits By similarity.
Subcellular location	Mitochondrion inner membraneBy similarity. Note= Matrix and cytoplasmic side of the mitochondrial inner membrane By similarity.
Sequence similarities	Belongs to the complex I 75 kDa subunit family. Contains 1 2Fe-2S ferredoxin-type domain.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	2Fe-2S 4Fe-4S Iron Iron-sulfur Metal-binding NAD Ubiquinone
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	ATP synthesis coupled electron transport Inferred from electronic annotation. Source: InterPro transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 675	675	NADH-ubiquinone oxidoreductase 75 kDa subunit		PRO_0000118536
Regions
	Domain	2 – 80	79	2Fe-2S ferredoxin-type
Sites
	Metal binding	36	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	47	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	50	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	64	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	96	1	Iron-sulfur 2 (4Fe-4S); via pros nitrogen By similarity
	Metal binding	100	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	103	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	109	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	148	1	Iron-sulfur 3 (4Fe-4S) By similarity
	Metal binding	151	1	Iron-sulfur 3 (4Fe-4S) By similarity
	Metal binding	154	1	Iron-sulfur 3 (4Fe-4S) By similarity
	Metal binding	198	1	Iron-sulfur 3 (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q37373-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 3870F3BC9E8B9807	FASTA	675	77,554
	10 20 30 40 50 60 MKNISFKVND FQYTINNKLT LIQACLKNKV DISRFCFHEK LSIAGNCRMC LVEDLKQVKP 70 80 90 100 110 120 LASCAINVSN SMNIYTNTLK VKKARESVLE FLLANHPLDC PICDQGGECD LQDQSVVFGS 130 140 150 160 170 180 DRGRFYEFKR SVEDKDCGPL IKTIMNRCIH CTRCVRFSNE VAGVNILGVT GRGSKMEIGF 190 200 210 220 230 240 YIENLMRSEL SGNVIDLCPV GALTSKPFAF TSRPWELKSY NSIDVLDSLH SNIRVDIRGT 250 260 270 280 290 300 KIMRILPRVN SELNEDWITD KIRFSYDSFR RQRLYDPMVK ISGSFLKIGW KKAMLFIKKF 310 320 330 340 350 360 FCNFLGFNHS SFIPLRGYIG DYLDLETIYT FKKFLLLNGS NFFLPSSSYN DLTALYSFNT 370 380 390 400 410 420 PLTRLDEGDF CILLDVNLRV ELPIVNSRIK QLVSKKMLPV FVLGFYSNFN YFVKHISNSS 430 440 450 460 470 480 KTLLHVLEGS HWLSAKISKK FSSKPIFLIG DSSSLLKGSL IVPLFNFTNV ICDNWNGLNI 490 500 510 520 530 540 ISNDSSYLST KEFNLSSSHS QNSHLLNFPI NFVLNYDKAV LVDSSAFQIY QGHHGDTNAI 550 560 570 580 590 600 NSNLIFPSTS FIEKNSFYSN SLAIVQKTKK ILFSPGNSRD DWKILNALID NFGFSYFKVR 610 620 630 640 650 660 NSFDLVSFLS ESTPFILYKR SFSFKCFGFY EQLVYHFFNY FSVNNNYYIY DSITRNSKIM 670 SLCFNKFKMK GYNFF

References

[1]

"The mitochondrial DNA of the amoeboid protozoon, Acanthamoeba castellanii: complete sequence, gene content and genome organization." Burger G., Plante I., Lonergan K.M., Gray M.W. J. Mol. Biol. 245:522-537(1995) [PubMed: 7844823] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 30010 / Neff.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U12386 Genomic DNA. Translation: AAD11824.1.
PIR	S53832.
RefSeq	NP_042531.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1734026.
Family and domain databases
InterPro	IPR006058. 2Fe2S_fd_BS. IPR001041. Ferredoxin. IPR006656. Mopterin_OxRdtase. IPR000283. NADH_DHase_75KDa_su_CS. IPR010228. NADH_quinone_OxRdtase_G. [Graphical view]
Pfam	PF00111. Fer2. 1 hit. PF00384. Molybdopterin. 1 hit. [Graphical view]
TIGRFAMs	TIGR01973. NuoG. 1 hit.
PROSITE	PS00197. 2FE2S_FER_1. False negative. PS51085. 2FE2S_FER_2. False negative. PS00641. COMPLEX1_75K_1. False negative. PS00642. COMPLEX1_75K_2. 1 hit. PS00643. COMPLEX1_75K_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDUS1_ACACA

Accession

Primary (citable) accession number: Q37373

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	November 25, 2008
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)