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Q37373

- NDUS1_ACACA

UniProt

Q37373 - NDUS1_ACACA

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Protein
NADH-ubiquinone oxidoreductase 75 kDa subunit
Gene
NAD11
Organism
Acanthamoeba castellanii (Amoeba)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity. This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized By similarity.

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

Binds 1 2Fe-2S cluster per subunit By similarity.
Binds 2 4Fe-4S clusters per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi36 – 361Iron-sulfur 1 (2Fe-2S) By similarity
Metal bindingi47 – 471Iron-sulfur 1 (2Fe-2S) By similarity
Metal bindingi50 – 501Iron-sulfur 1 (2Fe-2S) By similarity
Metal bindingi64 – 641Iron-sulfur 1 (2Fe-2S) By similarity
Metal bindingi96 – 961Iron-sulfur 2 (4Fe-4S); via pros nitrogen By similarity
Metal bindingi100 – 1001Iron-sulfur 2 (4Fe-4S) By similarity
Metal bindingi103 – 1031Iron-sulfur 2 (4Fe-4S) By similarity
Metal bindingi109 – 1091Iron-sulfur 2 (4Fe-4S) By similarity
Metal bindingi148 – 1481Iron-sulfur 3 (4Fe-4S) By similarity
Metal bindingi151 – 1511Iron-sulfur 3 (4Fe-4S) By similarity
Metal bindingi154 – 1541Iron-sulfur 3 (4Fe-4S) By similarity
Metal bindingi198 – 1981Iron-sulfur 3 (4Fe-4S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. NADH dehydrogenase (ubiquinone) activity Source: UniProtKB-EC
  4. electron carrier activity Source: InterPro
  5. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. ATP synthesis coupled electron transport Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-ubiquinone oxidoreductase 75 kDa subunit (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
Complex I-75kD
Short name:
CI-75kD
NADH dehydrogenase subunit 11
Gene namesi
Name:NAD11
Encoded oniMitochondrion
OrganismiAcanthamoeba castellanii (Amoeba)
Taxonomic identifieri5755 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaDiscoseaLongamoebiaCentramoebidaAcanthamoebidaeAcanthamoeba

Subcellular locationi

Mitochondrion inner membrane By similarity
Note: Matrix and cytoplasmic side of the mitochondrial inner membrane By similarity.

GO - Cellular componenti

  1. mitochondrial inner membrane Source: UniProtKB-SubCell
  2. respiratory chain Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 675675NADH-ubiquinone oxidoreductase 75 kDa subunit
PRO_0000118536Add
BLAST

Interactioni

Subunit structurei

Complex I is composed of about 30 different subunits By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ37373.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 80792Fe-2S ferredoxin-type
Add
BLAST
Domaini217 – 273574Fe-4S Mo/W bis-MGD-type
Add
BLAST

Sequence similaritiesi

Family and domain databases

InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR000283. NADH_UbQ_OxRdtase_75kDa_su_CS.
IPR010228. NADH_UbQ_OxRdtase_Gsu.
IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd.
[Graphical view]
PfamiPF00384. Molybdopterin. 2 hits.
PF10588. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view]
SMARTiSM00929. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view]
SUPFAMiSSF54292. SSF54292. 1 hit.
TIGRFAMsiTIGR01973. NuoG. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00642. COMPLEX1_75K_2. 1 hit.
PS00643. COMPLEX1_75K_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q37373-1 [UniParc]FASTAAdd to Basket

« Hide

MKNISFKVND FQYTINNKLT LIQACLKNKV DISRFCFHEK LSIAGNCRMC    50
LVEDLKQVKP LASCAINVSN SMNIYTNTLK VKKARESVLE FLLANHPLDC 100
PICDQGGECD LQDQSVVFGS DRGRFYEFKR SVEDKDCGPL IKTIMNRCIH 150
CTRCVRFSNE VAGVNILGVT GRGSKMEIGF YIENLMRSEL SGNVIDLCPV 200
GALTSKPFAF TSRPWELKSY NSIDVLDSLH SNIRVDIRGT KIMRILPRVN 250
SELNEDWITD KIRFSYDSFR RQRLYDPMVK ISGSFLKIGW KKAMLFIKKF 300
FCNFLGFNHS SFIPLRGYIG DYLDLETIYT FKKFLLLNGS NFFLPSSSYN 350
DLTALYSFNT PLTRLDEGDF CILLDVNLRV ELPIVNSRIK QLVSKKMLPV 400
FVLGFYSNFN YFVKHISNSS KTLLHVLEGS HWLSAKISKK FSSKPIFLIG 450
DSSSLLKGSL IVPLFNFTNV ICDNWNGLNI ISNDSSYLST KEFNLSSSHS 500
QNSHLLNFPI NFVLNYDKAV LVDSSAFQIY QGHHGDTNAI NSNLIFPSTS 550
FIEKNSFYSN SLAIVQKTKK ILFSPGNSRD DWKILNALID NFGFSYFKVR 600
NSFDLVSFLS ESTPFILYKR SFSFKCFGFY EQLVYHFFNY FSVNNNYYIY 650
DSITRNSKIM SLCFNKFKMK GYNFF 675
Length:675
Mass (Da):77,554
Last modified:November 1, 1996 - v1
Checksum:i3870F3BC9E8B9807
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U12386 Genomic DNA. Translation: AAD11824.1.
PIRiS53832.
RefSeqiNP_042531.1. NC_001637.1.

Genome annotation databases

GeneIDi1734026.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U12386 Genomic DNA. Translation: AAD11824.1 .
PIRi S53832.
RefSeqi NP_042531.1. NC_001637.1.

3D structure databases

ProteinModelPortali Q37373.
ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1734026.

Family and domain databases

InterProi IPR001041. 2Fe-2S_ferredoxin-type.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR000283. NADH_UbQ_OxRdtase_75kDa_su_CS.
IPR010228. NADH_UbQ_OxRdtase_Gsu.
IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd.
[Graphical view ]
Pfami PF00384. Molybdopterin. 2 hits.
PF10588. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view ]
SMARTi SM00929. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view ]
SUPFAMi SSF54292. SSF54292. 1 hit.
TIGRFAMsi TIGR01973. NuoG. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00642. COMPLEX1_75K_2. 1 hit.
PS00643. COMPLEX1_75K_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The mitochondrial DNA of the amoeboid protozoon, Acanthamoeba castellanii: complete sequence, gene content and genome organization."
    Burger G., Plante I., Lonergan K.M., Gray M.W.
    J. Mol. Biol. 245:522-537(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 30010 / Neff.

Entry informationi

Entry nameiNDUS1_ACACA
AccessioniPrimary (citable) accession number: Q37373
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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