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Q37373 (NDUS1_ACACA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADH-ubiquinone oxidoreductase 75 kDa subunit

EC=1.6.5.3
EC=1.6.99.3
Alternative name(s):
Complex I-75kD
Short name=CI-75kD
NADH dehydrogenase subunit 11
Gene names
Name:NAD11
Encoded onMitochondrion
OrganismAcanthamoeba castellanii (Amoeba)
Taxonomic identifier5755 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaDiscoseaLongamoebiaCentramoebidaAcanthamoebidaeAcanthamoeba

Protein attributes

Sequence length675 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity. This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized By similarity.

Catalytic activity

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).

NADH + acceptor = NAD+ + reduced acceptor.

Cofactor

Binds 1 2Fe-2S cluster per subunit By similarity.

Binds 2 4Fe-4S clusters per subunit By similarity.

Subunit structure

Complex I is composed of about 30 different subunits By similarity.

Subcellular location

Mitochondrion inner membrane By similarity. Note: Matrix and cytoplasmic side of the mitochondrial inner membrane By similarity.

Sequence similarities

Belongs to the complex I 75 kDa subunit family.

Contains 1 2Fe-2S ferredoxin-type domain.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 675675NADH-ubiquinone oxidoreductase 75 kDa subunit
PRO_0000118536

Regions

Domain2 – 80792Fe-2S ferredoxin-type
Domain217 – 273574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding361Iron-sulfur 1 (2Fe-2S) By similarity
Metal binding471Iron-sulfur 1 (2Fe-2S) By similarity
Metal binding501Iron-sulfur 1 (2Fe-2S) By similarity
Metal binding641Iron-sulfur 1 (2Fe-2S) By similarity
Metal binding961Iron-sulfur 2 (4Fe-4S); via pros nitrogen By similarity
Metal binding1001Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1031Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1091Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1481Iron-sulfur 3 (4Fe-4S) By similarity
Metal binding1511Iron-sulfur 3 (4Fe-4S) By similarity
Metal binding1541Iron-sulfur 3 (4Fe-4S) By similarity
Metal binding1981Iron-sulfur 3 (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q37373 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 3870F3BC9E8B9807

FASTA67577,554
        10         20         30         40         50         60 
MKNISFKVND FQYTINNKLT LIQACLKNKV DISRFCFHEK LSIAGNCRMC LVEDLKQVKP 

        70         80         90        100        110        120 
LASCAINVSN SMNIYTNTLK VKKARESVLE FLLANHPLDC PICDQGGECD LQDQSVVFGS 

       130        140        150        160        170        180 
DRGRFYEFKR SVEDKDCGPL IKTIMNRCIH CTRCVRFSNE VAGVNILGVT GRGSKMEIGF 

       190        200        210        220        230        240 
YIENLMRSEL SGNVIDLCPV GALTSKPFAF TSRPWELKSY NSIDVLDSLH SNIRVDIRGT 

       250        260        270        280        290        300 
KIMRILPRVN SELNEDWITD KIRFSYDSFR RQRLYDPMVK ISGSFLKIGW KKAMLFIKKF 

       310        320        330        340        350        360 
FCNFLGFNHS SFIPLRGYIG DYLDLETIYT FKKFLLLNGS NFFLPSSSYN DLTALYSFNT 

       370        380        390        400        410        420 
PLTRLDEGDF CILLDVNLRV ELPIVNSRIK QLVSKKMLPV FVLGFYSNFN YFVKHISNSS 

       430        440        450        460        470        480 
KTLLHVLEGS HWLSAKISKK FSSKPIFLIG DSSSLLKGSL IVPLFNFTNV ICDNWNGLNI 

       490        500        510        520        530        540 
ISNDSSYLST KEFNLSSSHS QNSHLLNFPI NFVLNYDKAV LVDSSAFQIY QGHHGDTNAI 

       550        560        570        580        590        600 
NSNLIFPSTS FIEKNSFYSN SLAIVQKTKK ILFSPGNSRD DWKILNALID NFGFSYFKVR 

       610        620        630        640        650        660 
NSFDLVSFLS ESTPFILYKR SFSFKCFGFY EQLVYHFFNY FSVNNNYYIY DSITRNSKIM 

       670 
SLCFNKFKMK GYNFF 

« Hide

References

[1]"The mitochondrial DNA of the amoeboid protozoon, Acanthamoeba castellanii: complete sequence, gene content and genome organization."
Burger G., Plante I., Lonergan K.M., Gray M.W.
J. Mol. Biol. 245:522-537(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 30010 / Neff.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U12386 Genomic DNA. Translation: AAD11824.1.
PIRS53832.
RefSeqNP_042531.1. NC_001637.1.

3D structure databases

ProteinModelPortalQ37373.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1734026.

Family and domain databases

InterProIPR001041. 2Fe-2S_ferredoxin-type.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR000283. NADH_UbQ_OxRdtase_75kDa_su_CS.
IPR010228. NADH_UbQ_OxRdtase_Gsu.
IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd.
[Graphical view]
PfamPF00384. Molybdopterin. 2 hits.
PF10588. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view]
SMARTSM00929. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view]
SUPFAMSSF54292. SSF54292. 1 hit.
TIGRFAMsTIGR01973. NuoG. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00642. COMPLEX1_75K_2. 1 hit.
PS00643. COMPLEX1_75K_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNDUS1_ACACA
AccessionPrimary (citable) accession number: Q37373
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families