Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q37373

- NDUS1_ACACA

UniProt

Q37373 - NDUS1_ACACA

Protein

NADH-ubiquinone oxidoreductase 75 kDa subunit

Gene

NAD11

Organism
Acanthamoeba castellanii (Amoeba)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity. This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized By similarity.By similarity

    Catalytic activityi

    NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
    NADH + acceptor = NAD+ + reduced acceptor.

    Cofactori

    Binds 1 2Fe-2S cluster per subunit.By similarity
    Binds 2 4Fe-4S clusters per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi36 – 361Iron-sulfur 1 (2Fe-2S)By similarity
    Metal bindingi47 – 471Iron-sulfur 1 (2Fe-2S)By similarity
    Metal bindingi50 – 501Iron-sulfur 1 (2Fe-2S)By similarity
    Metal bindingi64 – 641Iron-sulfur 1 (2Fe-2S)By similarity
    Metal bindingi96 – 961Iron-sulfur 2 (4Fe-4S); via pros nitrogenBy similarity
    Metal bindingi100 – 1001Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi103 – 1031Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi109 – 1091Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi148 – 1481Iron-sulfur 3 (4Fe-4S)By similarity
    Metal bindingi151 – 1511Iron-sulfur 3 (4Fe-4S)By similarity
    Metal bindingi154 – 1541Iron-sulfur 3 (4Fe-4S)By similarity
    Metal bindingi198 – 1981Iron-sulfur 3 (4Fe-4S)By similarity

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. electron carrier activity Source: InterPro
    4. metal ion binding Source: UniProtKB-KW
    5. NADH dehydrogenase (ubiquinone) activity Source: UniProtKB-EC

    GO - Biological processi

    1. ATP synthesis coupled electron transport Source: InterPro

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Respiratory chain, Transport

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADH-ubiquinone oxidoreductase 75 kDa subunit (EC:1.6.5.3, EC:1.6.99.3)
    Alternative name(s):
    Complex I-75kD
    Short name:
    CI-75kD
    NADH dehydrogenase subunit 11
    Gene namesi
    Name:NAD11
    Encoded oniMitochondrion
    OrganismiAcanthamoeba castellanii (Amoeba)
    Taxonomic identifieri5755 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaDiscoseaLongamoebiaCentramoebidaAcanthamoebidaeAcanthamoeba

    Subcellular locationi

    Mitochondrion inner membrane By similarity
    Note: Matrix and cytoplasmic side of the mitochondrial inner membrane.By similarity

    GO - Cellular componenti

    1. mitochondrial inner membrane Source: UniProtKB-SubCell
    2. respiratory chain Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 675675NADH-ubiquinone oxidoreductase 75 kDa subunitPRO_0000118536Add
    BLAST

    Interactioni

    Subunit structurei

    Complex I is composed of about 30 different subunits.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ37373.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 80792Fe-2S ferredoxin-typeAdd
    BLAST
    Domaini217 – 273574Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the complex I 75 kDa subunit family.Curated
    Contains 1 4Fe-4S Mo/W bis-MGD-type domain.PROSITE-ProRule annotation

    Family and domain databases

    InterProiIPR001041. 2Fe-2S_ferredoxin-type.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR000283. NADH_UbQ_OxRdtase_75kDa_su_CS.
    IPR010228. NADH_UbQ_OxRdtase_Gsu.
    IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd.
    [Graphical view]
    PfamiPF00384. Molybdopterin. 2 hits.
    PF10588. NADH-G_4Fe-4S_3. 1 hit.
    [Graphical view]
    SMARTiSM00929. NADH-G_4Fe-4S_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF54292. SSF54292. 1 hit.
    TIGRFAMsiTIGR01973. NuoG. 1 hit.
    PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00642. COMPLEX1_75K_2. 1 hit.
    PS00643. COMPLEX1_75K_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q37373-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKNISFKVND FQYTINNKLT LIQACLKNKV DISRFCFHEK LSIAGNCRMC    50
    LVEDLKQVKP LASCAINVSN SMNIYTNTLK VKKARESVLE FLLANHPLDC 100
    PICDQGGECD LQDQSVVFGS DRGRFYEFKR SVEDKDCGPL IKTIMNRCIH 150
    CTRCVRFSNE VAGVNILGVT GRGSKMEIGF YIENLMRSEL SGNVIDLCPV 200
    GALTSKPFAF TSRPWELKSY NSIDVLDSLH SNIRVDIRGT KIMRILPRVN 250
    SELNEDWITD KIRFSYDSFR RQRLYDPMVK ISGSFLKIGW KKAMLFIKKF 300
    FCNFLGFNHS SFIPLRGYIG DYLDLETIYT FKKFLLLNGS NFFLPSSSYN 350
    DLTALYSFNT PLTRLDEGDF CILLDVNLRV ELPIVNSRIK QLVSKKMLPV 400
    FVLGFYSNFN YFVKHISNSS KTLLHVLEGS HWLSAKISKK FSSKPIFLIG 450
    DSSSLLKGSL IVPLFNFTNV ICDNWNGLNI ISNDSSYLST KEFNLSSSHS 500
    QNSHLLNFPI NFVLNYDKAV LVDSSAFQIY QGHHGDTNAI NSNLIFPSTS 550
    FIEKNSFYSN SLAIVQKTKK ILFSPGNSRD DWKILNALID NFGFSYFKVR 600
    NSFDLVSFLS ESTPFILYKR SFSFKCFGFY EQLVYHFFNY FSVNNNYYIY 650
    DSITRNSKIM SLCFNKFKMK GYNFF 675
    Length:675
    Mass (Da):77,554
    Last modified:November 1, 1996 - v1
    Checksum:i3870F3BC9E8B9807
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U12386 Genomic DNA. Translation: AAD11824.1.
    PIRiS53832.
    RefSeqiNP_042531.1. NC_001637.1.

    Genome annotation databases

    GeneIDi1734026.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U12386 Genomic DNA. Translation: AAD11824.1 .
    PIRi S53832.
    RefSeqi NP_042531.1. NC_001637.1.

    3D structure databases

    ProteinModelPortali Q37373.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1734026.

    Family and domain databases

    InterProi IPR001041. 2Fe-2S_ferredoxin-type.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR000283. NADH_UbQ_OxRdtase_75kDa_su_CS.
    IPR010228. NADH_UbQ_OxRdtase_Gsu.
    IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd.
    [Graphical view ]
    Pfami PF00384. Molybdopterin. 2 hits.
    PF10588. NADH-G_4Fe-4S_3. 1 hit.
    [Graphical view ]
    SMARTi SM00929. NADH-G_4Fe-4S_3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54292. SSF54292. 1 hit.
    TIGRFAMsi TIGR01973. NuoG. 1 hit.
    PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00642. COMPLEX1_75K_2. 1 hit.
    PS00643. COMPLEX1_75K_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The mitochondrial DNA of the amoeboid protozoon, Acanthamoeba castellanii: complete sequence, gene content and genome organization."
      Burger G., Plante I., Lonergan K.M., Gray M.W.
      J. Mol. Biol. 245:522-537(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 30010 / Neff.

    Entry informationi

    Entry nameiNDUS1_ACACA
    AccessioniPrimary (citable) accession number: Q37373
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 91 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3